在这项研究中,一种快速、简单、准确和敏感的亲水交互液相色谱(HILIC)方法建立了同时测定的自由氨基酸的浮萍,包括在三个不同的品种Spirodela polyrhiza(l)斯克莱德。Landoltia punctata(g .最大经济产量)。Les & d·j·克劳福德浮萍属aequinoctialisWelwitsch通过超高液相色谱法耦合串联质谱(UHPLC-MS / MS)。方法验证的线性处理,精度、稳定性、可重复性和准确性以及检测和量化的极限。量化的开发方法应用59批次的样品。然后最优化分析用于评估不同浮萍通过主成分分析(PCA)和正交偏最小squares-discriminant分析(OPLS-DA)。结果表明,没有显著差异法斯的配置文件有三种浮萍品种。
1。介绍
氨基酸种类的含氮代谢中扮演至关重要的和多样化的角色的组件,并吸引了大量关注,食品,饲料,滋养补品。氨基酸是蛋白质的基本单位和重要成分和参与进步糖蛋白的生物合成卟啉,神经递质,多胺和一氧化氮1- - - - - -5]。营养学研究表明,氨基酸可以调节基因表达和增强骨骼肌的生长和小肠(6]。
浮萍科俗称浮萍的家庭长期以来一直作为人类食品消费(7]。浮萍有爆发性繁殖的引人注目的能力8]。他们几乎在任何地方都能生长在适当的温度和营养水。但他们最大的潜力是产生大量的富含蛋白质的生物量,对人类是一种很有前途的食物资源9),适合饲养各种动物包括鱼类,家禽和牛10- - - - - -12]。的内容自由氨基酸(FAAs)的评估是非常重要的富含蛋白质的食物。新鲜的浮萍包含大量的水与水分含量的86% - -94%。但干样品,他们的蛋白质浓度范围从25%到40%,比这低得多的纤维材料的土地饲料(13]。的栖息地大大影响蛋白质和纤维的内容。氨基酸和蛋白质含量总是依赖他们的物种模式和成长环境,如阳光,营养媒体,和他们的增长模式:群居或孤独。在高营养条件下,更多的蛋白质和更少的纤维堆积在浮萍,反之亦然。浮萍的氨基酸类型非常接近的动物,因此可能被动物利用效率(7,14]。第一限制氨基酸(赖氨酸)类似于大豆和更优于其他食品如高粱和玉米。
有几种方法在文献报道自由氨基酸的分析。一般来说,与溶剂提取法水,甲酸,盐酸,或乙醇,紧随其后的是过滤或离心之前分析(15- - - - - -17]。描述了几个技术检测自由氨基酸包括离子交换色谱法(18),precolumn衍生紧随其后的是反向高效液相色谱法加上二极管阵列或荧光检测器19,20.),气相色谱,质谱、毛细管电泳(21]。近年来,使用超高液相色谱法测定用连字符连接质谱分析获得了伟大的注意,因为选择性分离的优点的极地underivatized HILIC条件下分析物与acetonitrile-dominated流动相(22- - - - - -25]。
在目前的研究中,一个可行的和可靠的UPLC-QTRAP-MS / MS方法开发和验证的定量分析24 underivatized同时法斯。多元统计分析是用来评估资料的差异法斯在三种浮萍。这个简单和快速分析方法可用于质量控制的浮萍。
2。材料和方法
2.1。试剂和材料
收集59批次的浮萍在中国不同地区如表所示1。聚合酶链反应(样本识别和分类26- - - - - -28),人口的数量是如下:25的数量由物种Spirodela polyrhiza从物种,16个人口Landoltia punctata之前(它的另一个无效的名字Spirodela oligorrhizaKurz Hegelm。), 18岁人群浮萍属aequinoctialis。新鲜的材料被自来水清洗,干在阳光下,和粉,粉是通过60-mesh筛筛选的。参考标准的酪氨酸(酪氨酸),丙氨酸(Ala),谷氨酸(Glu),苯丙氨酸(检),组氨酸(他的),异亮氨酸(Ile)、天冬氨酸(Asp)、赖氨酸(赖氨酸),甘氨酸(g)、缬氨酸(Val),瓜氨酸(Cit),胱氨酸(Cys2),脯氨酸(Pro),色氨酸(Trp),亮氨酸(低浓缩铀),精氨酸(Arg),半胱氨酸(半胱氨酸)hydroxylproline (Hpro),蛋氨酸(遇到),苏氨酸(刺)、天冬酰胺(Asn)和丝氨酸(Ser)从国立购买食品和药物控制(中国,北京)纯度超过98%。参考的标准γ氨基丁酸(GABA)和谷氨酰胺(Gln)纯度超过98%得到从阿拉丁工业公司(上海,中国)。乙腈(高效液相色谱级)购买从默克(达姆施塔特,德国)。甲酸和甲酸铵(高效液相色谱级)是由Mreda技术公司(美国)。去离子水用于分析过程是由一个Milli-Q学术超纯水系统(微孔,贝德福德,妈,美国)。
|
| 样本 |
物种 |
网站 |
|
| SP1-7 |
Spirodela polyrhiza |
江苏省扬州市 |
| SP8-9 |
Spirodela polyrhiza |
江苏省盐城市 |
| SP10-11 |
Spirodela polyrhiza |
江苏省南京市 |
| SP12-13 |
Spirodela polyrhiza |
江苏省淮安城市 |
| SP14-17 |
Spirodela polyrhiza |
江苏省连云港城市 |
| SP18-19 |
Spirodela polyrhiza |
山东省临沂市 |
| SP20-21 |
Spirodela polyrhiza |
安徽省合肥城市 |
| SP22-23 |
Spirodela polyrhiza |
杭州城市江苏省 |
| SP24-25 |
Spirodela polyrhiza |
河北省保定市 |
| LP1-11 |
Landoltia punctata |
江苏省扬州市 |
| LP12-16 |
Landoltia punctata |
江苏省淮安城市 |
| LA1-3 |
浮萍属aequinoctialis |
江苏省扬州市 |
| LA4-8 |
浮萍属aequinoctialis |
江苏省盐城市 |
| LA9-14 |
浮萍属aequinoctialis |
江苏省南京市 |
| LA15-16 |
浮萍属aequinoctialis |
江苏省连云港城市 |
| LA17-18 |
浮萍属aequinoctialis |
山东省临沂市 |
|
|
2.2。仪表和色谱和质量状况
色谱分析进行一个日本岛津公司LC-30超高液相色谱系统(日本岛津公司、日本),由一个通信总线模块(CMB-20A),真空脱气装置,二元梯度泵(LC-30AD),一个autosampler (SIL-30A)和柱温箱(CTO-30A)加上一个AB Sciex QTRAP 5500(美国AB Sciex)。水域XBridge酰胺列(2.1毫米×150毫米,2.5μ米,水域,美国)用于色谱分离。二元流动相是由乙腈(A)和10毫米甲酸铵为0.2% (v / v)甲酸(B)在0.4毫升/分钟的流量。线性梯度洗脱进行如下:0 - 5分钟,90% -85.5%;5-11 min, 85.5% - -54%;11 - 12分钟,54% - -10%;12 - 14分钟,10%;14日至15日,10% -90%;15 - 20分钟,90%的reequilibration时间是4分钟总运行时间20分钟。柱室是保持在20°C,而autosampler审判是维持在15°C,和注射量是2μl。针被乙腈和水的混合物。
质谱分析是一个三重四质谱仪配有电喷雾电离源。阳离子类型下的光谱记录多反应监测(MRM)模式。毛细管电压是5500 V,反溶剂气体温度为550°C。窗帘气体是35 psi喷雾器和干燥气体的55 psi。氮作为源气体的纯度超过95%和碰撞气体纯度超过99.999%。24个氨基酸成分优化与质量优化模式只获得前体离子和产品的质量,最好的declustering潜在(DP),分别和碰撞能量(CE)。细节如表所示2。入口处电位(EP)和碰撞细胞退出的可能性(级)被设置为默认值。典型的LC-QTRAP-MS / MS色谱图所示1。
|
| 数量 |
复合 |
前体离子 |
产品离子 |
DP (V) |
CE (V) |
|
| 1 |
酪氨酸 |
182.0 |
91.1 |
60 |
37 |
| 2 |
阿拉巴马州 |
90.0 |
44.1 |
50 |
14 |
| 3 |
Glu |
148.1 |
84.0 |
60 |
21 |
| 4 |
板式换热器 |
166.1 |
120.1 |
60 |
19 |
| 5 |
他的 |
156.1 |
110.3 |
80年 |
20. |
| 6 |
Ile |
132.1 |
86.1 |
50 |
14 |
| 7 |
Asp |
134.1 |
74.0 |
50 |
19 |
| 8 |
利斯河 |
147.0 |
84.1 |
60 |
23 |
| 9 |
通用电气 |
76.1 |
29.9 |
40 |
16 |
| 10 |
瓦尔 |
118.1 |
71.9 |
50 |
15 |
| 11 |
Cit |
176.2 |
70.0 |
60 |
31日 |
| 12 |
伽马氨基丁酸 |
104.1 |
86.8 |
50 |
14 |
| 13 |
Cys2 |
241.1 |
73.9 |
80年 |
40 |
| 14 |
箴 |
116.0 |
70.1 |
60 |
22 |
| 15 |
Trp |
205.1 |
146.0 |
50 |
24 |
| 16 |
低浓缩铀 |
132.1 |
86.1 |
50 |
14 |
| 17 |
参数 |
175.2 |
70.2 |
90年 |
30. |
| 18 |
半胱氨酸 |
122.1 |
59.0 |
40 |
32 |
| 19 |
Hpro |
132.1 |
86.1 |
60 |
20. |
| 20. |
见过 |
150.1 |
103.9 |
60 |
14 |
| 21 |
用力推 |
120.1 |
74.1 |
40 |
14 |
| 22 |
Asn |
133.1 |
74.0 |
60 |
21 |
| 23 |
Gln |
147.0 |
84.1 |
60 |
23 |
| 24 |
爵士 |
106.0 |
60.1 |
60 |
15 |
|
|
2.3。制备复合解决方案的参考
股票的解决方案的个人标准是由精确称重元件溶解在0.5%甲酸浓度为0.2毫克/毫升约在4°C和存储使用。混合工作解决方案的所有标准稀释0.5%甲酸之前立即解决一系列适当的浓度分析和用于实现校准曲线。
2.4。样品溶液的制备
每个样品粉重达0.2 g准确和转移到25毫升不锈钢管与6不锈钢珠直径0.6毫米。然后,样品提取高通量tissue-grinding器与振动频率的70 kHz和15毫升0.5%甲酸120年代。示例解决方案是在15000转离心10分钟,然后上层清液通过0.22上映μm聚四氟乙烯膜过滤和储存在一个玻璃瓶在4°C后质/ MS分析。
2.5。方法验证
对于每个参考物,证实了校准曲线线性回归的峰面积和浓度。检测的局限性(LOD)和量化的极限(定量限)是由稀释标准溶液到信噪比率是3和10个,分别。精度是由盘中,interday可变性。盘中变异性是由确定相同的标准溶液在六个复制在同一天。interday变异性是由连续三天确定相同的解决方案。相对标准偏差(RSD)值是表示精度计算。样品溶液的稳定性进行了分析分析物的峰面积(0,2、4、8、12、24小时在室温下。评估的重复性开发方法,相同的样本分析六个复制和变异被表示为相对标准偏差。恢复测试进行评估的准确性的方法。测试,一个已知数量的24个标准组件上升到一定程度(0.1 g)的样本。 Then, the spiked sample was extracted and analysed as described. The recovery rate was calculated by using the following formula: recovery (%) = (measured amount − original amount)/spiked amount × 100%. Six replicates were performed for the recovery test.
2.6。数据分析
获得数据的定量分析是处理MultiQuant 3.0.2(美国AB SCIEX)。使用多元统计分析包括PCA和OPLS-DA对样本进行分类。SIMCA-P 14.1 (Umetrics AB、瑞典)被用来进行数据的统计分析59批次的浮萍。
2.7。矿物质和总蛋白质的分析
24矿物质的浮萍居住环境水被icp量化。水体的总氮和磷测定根据碱性过硫酸钾消解方法gb11894 - 89和钼酸铵分光光度法测定gb11893 - 89中国国家技术监督局发布的。总蛋白质的浮萍凯氏法测定。每个样本的浮萍在酸性条件下水解,然后总氨基酸被HPLC-MSMS量化方法上面提到的。
3所示。结果与讨论
3.1。优化的提取过程
免费的氨基酸可以溶于甲醇、乙醇、水、稀酸、等等。为了获得一个有效的提取策略浮萍的氨基酸,变量的提取过程进行调查。溶剂(甲醇、乙醇、水、0.1%甲酸和甲酸0.5%)、方法(加热回流、超声破碎法,tissue-grinding)和时间(20、30和40分钟声波降解法;60、90和120年代tissue-grinding)进行了研究。结果表明,氨基酸的数量没有显著差异的声波降解法时间30分钟在0.5%甲酸和120年代tissue-grinding时间在0.5%甲酸。提取的金额并不多,通过其他方法这两种形式。从工作效率的角度,tissue-grinding方法比声波降解法更省时的方法提取时间的120年代和30分钟。
3.2。优化UPLC条件
色谱条件的优化来达到一个令人满意的分离氨基酸特别是异构分子。列(水域XBridge酰胺,2.5μ120米,2.1×150毫米和安捷伦Poroshell HILIC, 2.7μ米,2.1×150毫米),列温度(20、25、30、35和40°C),流动相(乙腈- 0.1%甲酸,甲酸乙腈- 0.2%,与0.2%的甲酸乙腈- 0.2%甲酸,甲酸和甲酸铵acetonitrile-10毫米0.2%),和不同的梯度洗脱。
结果表明,氨基酸的峰形状成了好当甲酸浓度的上升,但保留时间明显缩短特别是一些甲酸补充到乙腈。甲酸铵等缓冲增加氨基酸的决议度。更好的保留时间和分离观察进行分析水域酰胺列比在120年安捷伦Poroshell HILIC列,并得出最佳的色谱与温度控制在20°C。尤其是对亮氨酸和异亮氨酸的同分异构体,成功实现基线分离时使用流动相组成的acetonitrile-10 mM甲酸铵0.2%甲酸。在其他上述条件下,这两个异构氨基酸分离相互地并不好。
3.3。MS / MS的优化条件
主要实验获得最好的质谱条件单独的氨基酸。为此,每个引用组件的氨基酸的浓度近似100 ng / mL被交付到质谱仪直接通过注射泵的速度7μL / min。调优标准溶解在水中时,大多数氨基酸的反应很低sodium-adducted离子时,[M + Na]+是相对较高的。相反,当溶解标准在0.1%或更高浓度的甲酸溶液,使质子化的反应分子,[M + H]+,显著高于metal-adducted离子几乎没有观察到。因此,前体离子(Q1)、产品离子(Q3) declustering潜在(DP)和碰撞能量(CE)的每个氨基酸和优化。
3.4。方法验证
如表所示324个氨基酸的校准曲线显示,良好的线性回归(所有相关系数> 0.995)在一个广泛的浓度。每个分析物的LOD和定量限的信噪比(S / N) 3和10的范围在0.37 - -1.94 ng / mL和0.74 - -4.49 ng / mL,分别。盘中的RSD值和interday精度表进行了总结4。的结果,目前的方法被发现精确的盘中变化小于3.14%,interday变化小于4.27%。示例解决方案是稳定在24 h和稳定RSD < 3.82%。重复性的相对标准偏差值是0.52% - -3.86%。24个氨基酸的回收率介于95.27% - -107.51%,RSD < 4.03%。
|
| 数量 |
复合 |
校准曲线 |
R2 |
线性范围(ng / mL) |
定量限(ng / mL) |
LOD (ng / mL) |
|
| 1 |
酪氨酸 |
y= 6534.1x+ 6063.4 |
0.9984 |
-5780 - 1.16 |
0.92 |
0.46 |
| 2 |
阿拉巴马州 |
y= 7168.9x+ 4932.8 |
0.9969 |
-5300 - 5.30 |
3.71 |
1.24 |
| 3 |
Glu |
y= 12796x+ 47334 |
0.9960 |
-2564 - 6.41 |
4.49 |
1.50 |
| 4 |
板式换热器 |
y= 59157x+ 42917 |
0.9965 |
0.99 - -992.0 |
0.79 |
0.40 |
| 5 |
他的 |
y= 20263x+ 247917 |
0.9962 |
-5570 - 5.57 |
3.90 |
1.30 |
| 6 |
Ile |
y= 70857x+ 46587 |
0.9957 |
-1074 - 1.07 |
0.86 |
0.43 |
| 7 |
Asp |
y= 7084.7x+ 86152 |
0.9963 |
-5430 - 5.43 |
3.80 |
1.27 |
| 8 |
利斯河 |
y= 4305.1x+ 17648 |
0.9980 |
-5530 - 5.53 |
3.87 |
1.94 |
| 9 |
通用电气 |
y= 1063.0x+ 3407.3 |
0.9990 |
-4680 - 0.94 |
0.75 |
0.37 |
| 10 |
瓦尔 |
y= 31972x+ 35728 |
0.9962 |
-1112 - 1.11 |
0.89 |
0.44 |
| 11 |
Cit |
y= 8520.8x+ 9811.0 |
0.9980 |
-5070 - 1.01 |
0.81 |
0.41 |
| 12 |
伽马氨基丁酸 |
y= 18495x+ 14623 |
0.9981 |
-5490 - 1.10 |
0.88 |
0.44 |
| 13 |
Cys2 |
y= 2389.7x+ 3146.6 |
0.9978 |
-5440 - 1.09 |
0.87 |
0.44 |
| 14 |
箴 |
y= 51204x+ 58539 |
0.9981 |
1.02 - -510.0 |
0.82 |
0.41 |
| 15 |
Trp |
y= 19030x+ 4465.3 |
0.9977 |
-2476 - 1.24 |
0.99 |
0.50 |
| 16 |
低浓缩铀 |
y= 56715x+ 40047 |
0.9974 |
-1012 - 1.01 |
0.81 |
0.40 |
| 17 |
参数 |
y= 11505x+ 84828 |
0.9974 |
-5960 - 5.96 |
4.17 |
1.39 |
| 18 |
半胱氨酸 |
y= 2496.6x+ 1017.5 |
0.9988 |
-6012 - 1.20 |
0.96 |
0.48 |
| 19 |
Hpro |
y= 15783x+ 3968.1 |
0.9977 |
-1892 - 0.95 |
0.76 |
0.38 |
| 20. |
见过 |
y= 8626.4x+ 1545.9 |
0.9984 |
-5250 - 1.05 |
0.84 |
0.42 |
| 21 |
用力推 |
y= 8807.8x+ 19073 |
0.9957 |
-4460 - 4.46 |
3.12 |
1.56 |
| 22 |
Asn |
y= 4939.9x+ 22367 |
0.9967 |
-5980 - 1.20 |
0.96 |
0.48 |
| 23 |
Gln |
y= 7733.9x+ 8338.4 |
0.9961 |
-4650 - 0.93 |
0.74 |
0.37 |
| 24 |
爵士 |
y= 7731.3x+ 54859 |
0.9977 |
-5090 - 5.09 |
3.56 |
1.19 |
|
|
|
| 数量 |
复合 |
精度 |
稳定的相对标准偏差(%) |
可重复性相对标准偏差(%) |
精度 |
| 盘中相对标准偏差(%) |
Interday相对标准偏差(%) |
恢复(%) |
相对标准偏差(%) |
|
| 1 |
酪氨酸 |
1.36 |
1.85 |
0.94 |
3.05 |
98.56 |
1.33 |
| 2 |
阿拉巴马州 |
2.60 |
3.31 |
3.47 |
3.09 |
102.49 |
2.18 |
| 3 |
Glu |
2.07 |
2.56 |
2.10 |
2.11 |
106.94 |
3.76 |
| 4 |
板式换热器 |
0.66 |
1.03 |
2.60 |
1.66 |
97.23 |
4.03 |
| 5 |
他的 |
1.07 |
3.30 |
1.58 |
1.17 |
98.32 |
2.20 |
| 6 |
Ile |
0.97 |
3.21 |
3.82 |
0.71 |
99.09 |
3.57 |
| 7 |
Asp |
1.96 |
3.98 |
2.42 |
1.84 |
97.86 |
2.36 |
| 8 |
利斯河 |
2.70 |
3.51 |
1.44 |
0.99 |
102.30 |
3.02 |
| 9 |
通用电气 |
1.66 |
2.65 |
3.43 |
3.74 |
104.48 |
3.07 |
| 10 |
瓦尔 |
2.42 |
2.89 |
3.30 |
0.83 |
107.51 |
1.52 |
| 11 |
Cit |
1.47 |
1.88 |
3.12 |
3.86 |
99.14 |
2.21 |
| 12 |
伽马氨基丁酸 |
3.14 |
2.32 |
2.27 |
0.82 |
102.54 |
3.41 |
| 13 |
Cys2 |
2.53 |
4.27 |
3.24 |
1.49 |
96.60 |
1.27 |
| 14 |
箴 |
1.21 |
3.39 |
1.09 |
2.09 |
101.94 |
3.20 |
| 15 |
Trp |
0.37 |
3.93 |
1.36 |
1.39 |
99.11 |
3.46 |
| 16 |
低浓缩铀 |
1.50 |
3.90 |
3.40 |
1.50 |
95.27 |
3.61 |
| 17 |
参数 |
2.72 |
4.15 |
3.72 |
1.45 |
102.37 |
1.79 |
| 18 |
半胱氨酸 |
2.82 |
3.63 |
1.16 |
0.53 |
103.65 |
3.82 |
| 19 |
Hpro |
3.05 |
2.23 |
0.87 |
2.38 |
97.55 |
3.91 |
| 20. |
见过 |
1.57 |
3.02 |
1.26 |
0.52 |
104.89 |
2.14 |
| 21 |
用力推 |
2.11 |
2.13 |
1.77 |
3.01 |
95.97 |
3.00 |
| 22 |
Asn |
2.93 |
2.85 |
1.48 |
3.68 |
105.35 |
2.95 |
| 23 |
Gln |
0.97 |
1.87 |
1.22 |
2.72 |
102.33 |
2.16 |
| 24 |
爵士 |
2.71 |
2.86 |
0.98 |
1.48 |
97.44 |
1.88 |
|
|
3.5。定量分析样品
发达UPLC-MS申请/ MS方法同时测定24自由氨基酸在59批次的三种浮萍。所有的内容都显示在表中5。结果表明,氨基酸的总量急剧变化中每个样本来自不同地区和物种。总含量的原子吸收光谱法在SP25河北省保定市高达1899.50μg / g,更比SP17 166.05μ从江苏省连云港城市g / g。对于每一个氨基酸,内容也即使在相同的样本之间存在着显著的差异。巧合的是,自由氨基酸的内容,包括在其分子中硫的比别人少得多,包括半胱氨酸、胱氨酸、蛋氨酸浓度为0.24,4.25和4.20μg / g的最大分别。特别是对于半胱氨酸,它是没有找到的Spirodela polyrhiza只有几个样品中发现的其他两个物种。半胱氨酸的假定的原因是硫醇是不稳定的,容易被氧化。但总的来说,没有显著差异在不同物种之间的所有法斯的总含量。
|
| 样本 |
酪氨酸 |
阿拉巴马州 |
Glu |
板式换热器 |
他的 |
Ile |
Asp |
利斯河 |
通用电气 |
| 免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
|
| SP1 |
3.16 |
6259.48 |
0.0505 |
6.95 |
10680.18 |
0.0651 |
25.27 |
16774.31 |
0.1506 |
0.44 |
9597.56 |
0.0046 |
1.52 |
4255.26 |
0.0357 |
0.38 |
8532.07 |
0.0045 |
9.31 |
11169.49 |
0.0834 |
11.95 |
7164.09 |
0.1668 |
3.33 |
8764.89 |
0.0380 |
| SP2 |
6.73 |
6161.25 |
0.1092 |
65.17 |
10089.59 |
0.6459 |
54.61 |
25366.04 |
0.2153 |
0.95 |
10729.73 |
0.0089 |
4.43 |
3888.26 |
0.1139 |
1.35 |
10094.12 |
0.0134 |
56.68 |
15862.58 |
0.3573 |
21.28 |
10090.45 |
0.2109 |
8.38 |
8695.46 |
0.0964 |
| SP3 |
39.72 |
6115.87 |
0.6495 |
173.11 |
9881.22 |
1.7519 |
96.43 |
15463.41 |
0.6236 |
7.97 |
8667.06 |
0.0920 |
22.26 |
3824.22 |
0.5821 |
11.90 |
7226.86 |
0.1647 |
69.12 |
12387.34 |
0.5580 |
112.90 |
8153.11 |
1.3847 |
128.27 |
6293.65 |
2.0381 |
| SP4 |
29.21 |
6251.45 |
0.4673 |
157.69 |
12424.62 |
1.2692 |
49.52 |
22986.51 |
0.2154 |
5.01 |
10467.29 |
0.0479 |
32.80 |
3569.79 |
0.9188 |
11.14 |
8392.28 |
0.1327 |
170.28 |
17175.49 |
0.9914 |
137.48 |
10225.20 |
1.3445 |
78.17 |
9626.14 |
0.8121 |
| SP5 |
5.58 |
6257.00 |
0.0892 |
29.04 |
13866.45 |
0.2094 |
34.93 |
23518.46 |
0.1485 |
0.86 |
11427.08 |
0.0075 |
6.41 |
4312.95 |
0.1486 |
0.96 |
9753.24 |
0.0098 |
16.39 |
18654.70 |
0.0879 |
21.25 |
11094.76 |
0.1915 |
5.53 |
10174.01 |
0.0544 |
| SP6 |
27.42 |
6245.68 |
0.4390 |
273.35 |
12005.84 |
2.2768 |
49.09 |
18787.83 |
0.2613 |
2.55 |
10284.72 |
0.0248 |
45.46 |
3424.75 |
1.3274 |
13.55 |
8465.53 |
0.1601 |
132.84 |
15752.94 |
0.8433 |
139.27 |
8608.52 |
1.6178 |
77.36 |
7010.22 |
1.1035 |
| SP7 |
39.61 |
5884.11 |
0.6732 |
229.20 |
12123.87 |
1.8905 |
71.86 |
18146.81 |
0.3960 |
4.90 |
8585.01 |
0.0571 |
46.17 |
4088.80 |
1.1292 |
11.22 |
7231.96 |
0.1551 |
94.84 |
14833.85 |
0.6393 |
230.52 |
7636.34 |
3.0187 |
65.35 |
5417.04 |
1.2064 |
| SP8 |
5.71 |
6124.81 |
0.0932 |
38.00 |
9734.57 |
0.3904 |
53.14 |
17640.33 |
0.3012 |
1.17 |
8527.84 |
0.0137 |
4.56 |
3751.52 |
0.1216 |
1.40 |
7266.84 |
0.0193 |
42.82 |
14315.75 |
0.2991 |
22.77 |
7392.06 |
0.3080 |
5.63 |
7832.50 |
0.0719 |
| SP9 |
13.90 |
6054.66 |
0.2296 |
120.58 |
9953.18 |
1.2115 |
70.75 |
16620.79 |
0.4257 |
3.68 |
8384.54 |
0.0439 |
18.01 |
3496.99 |
0.5150 |
5.25 |
6745.54 |
0.0778 |
94.10 |
11606.55 |
0.8107 |
73.99 |
6323.41 |
1.1701 |
21.63 |
7899.86 |
0.2738 |
| SP10 |
16.70 |
6097.17 |
0.2739 |
118.61 |
13272.16 |
0.8937 |
74.72 |
25451.33 |
0.2936 |
3.72 |
11056.99 |
0.0336 |
28.19 |
4110.01 |
0.6859 |
4.41 |
9671.14 |
0.0456 |
36.11 |
17757.11 |
0.2034 |
70.81 |
8546.75 |
0.8285 |
25.55 |
7505.72 |
0.3404 |
| SP11 |
23.10 |
6004.10 |
0.3847 |
205.08 |
11155.38 |
1.8384 |
67.31 |
26088.53 |
0.2580 |
10.26 |
9883.88 |
0.1038 |
61.35 |
3238.60 |
1.8943 |
14.23 |
8891.25 |
0.1600 |
56.14 |
15601.46 |
0.3598 |
133.66 |
8386.70 |
1.5937 |
87.43 |
9088.41 |
0.9620 |
| SP12 |
9.38 |
6127.68 |
0.1531 |
93.06 |
9760.92 |
0.9534 |
51.58 |
26516.14 |
0.1945 |
7.60 |
7997.37 |
0.0950 |
31.87 |
3300.60 |
0.9656 |
4.09 |
9260.26 |
0.0442 |
225.20 |
12413.46 |
1.8142 |
74.86 |
5684.24 |
1.3170 |
24.76 |
6908.86 |
0.3584 |
| SP13 |
93.61 |
6130.06 |
1.5271 |
195.69 |
8956.55 |
2.1849 |
52.85 |
16377.44 |
0.3227 |
23.33 |
7668.17 |
0.3042 |
46.78 |
2889.26 |
1.6191 |
14.85 |
7650.07 |
0.1941 |
4.76 |
10136.24 |
0.0470 |
135.91 |
6635.82 |
2.0481 |
73.19 |
5663.18 |
1.2924 |
| SP14 |
19.98 |
6120.49 |
0.3264 |
161.86 |
13337.15 |
1.2136 |
96.09 |
15114.77 |
0.6357 |
5.94 |
10678.41 |
0.0556 |
26.03 |
4189.64 |
0.6213 |
6.56 |
9933.71 |
0.0660 |
29.20 |
13225.32 |
0.2208 |
102.55 |
11141.92 |
0.9204 |
34.50 |
7778.37 |
0.4435 |
| SP15 |
1.13 |
6057.44 |
0.0187 |
19.99 |
13617.28 |
0.1468 |
17.01 |
21045.25 |
0.0808 |
0.62 |
11056.19 |
0.0056 |
9.77 |
4213.32 |
0.2319 |
0.30 |
8452.34 |
0.0035 |
0.95 |
12630.34 |
0.0075 |
17.70 |
8018.34 |
0.2207 |
6.64 |
6872.17 |
0.0966 |
| SP16 |
61.29 |
5884.22 |
1.0416 |
280.92 |
12551.36 |
2.2382 |
46.82 |
20775.77 |
0.2254 |
19.27 |
10311.88 |
0.1869 |
54.60 |
3881.89 |
1.4065 |
20.18 |
9290.92 |
0.2172 |
112.81 |
13791.37 |
0.8180 |
158.24 |
7293.79 |
2.1695 |
52.41 |
8307.13 |
0.6309 |
| SP17 |
0.89 |
5938.26 |
0.0150 |
23.96 |
10170.30 |
0.2356 |
46.90 |
15656.17 |
0.2996 |
0.42 |
10562.87 |
0.0040 |
2.49 |
5358.17 |
0.0465 |
1.11 |
10201.54 |
0.0109 |
2.95 |
12263.36 |
0.0241 |
13.08 |
8069.24 |
0.1621 |
4.44 |
5669.36 |
0.0783 |
| SP18 |
12.28 |
5985.32 |
0.2052 |
157.75 |
13673.70 |
1.1537 |
81.27 |
15272.53 |
0.5321 |
15.58 |
10449.06 |
0.1491 |
28.93 |
4949.42 |
0.5845 |
10.04 |
9948.89 |
0.1009 |
85.38 |
20641.51 |
0.4136 |
79.39 |
11463.15 |
0.6926 |
29.19 |
6848.95 |
0.4262 |
| SP19 |
45.66 |
5897.55 |
0.7742 |
182.98 |
12217.80 |
1.4977 |
87.43 |
20888.93 |
0.4185 |
28.84 |
10128.19 |
0.2847 |
48.02 |
4231.77 |
1.1347 |
20.85 |
9161.44 |
0.2276 |
10.22 |
14574.99 |
0.0701 |
83.12 |
9060.52 |
0.9174 |
34.17 |
6664.53 |
0.5127 |
| SP20 |
28.03 |
5860.01 |
0.4783 |
241.91 |
9302.34 |
2.6005 |
46.17 |
14837.19 |
0.3112 |
23.18 |
7603.97 |
0.3048 |
64.10 |
3206.91 |
1.9988 |
14.80 |
6774.94 |
0.2185 |
109.03 |
11544.71 |
0.9444 |
164.87 |
6737.28 |
2.4471 |
73.15 |
6715.81 |
1.0892 |
| SP21 |
2.75 |
6264.80 |
0.0439 |
55.55 |
10989.25 |
0.5055 |
91.79 |
22493.65 |
0.4081 |
0.84 |
9615.10 |
0.0087 |
23.80 |
4257.07 |
0.5591 |
1.00 |
8364.75 |
0.0120 |
- - - - - - |
11442.81 |
- - - - - - |
31.30 |
6932.76 |
0.4515 |
9.46 |
6910.06 |
0.1369 |
| SP22 |
7.95 |
5839.15 |
0.1361 |
29.20 |
10343.17 |
0.2823 |
22.86 |
16692.99 |
0.1369 |
1.07 |
8957.29 |
0.0119 |
5.17 |
3585.28 |
0.1442 |
1.06 |
8011.79 |
0.0132 |
0.91 |
13590.39 |
0.0067 |
15.15 |
6361.56 |
0.2381 |
11.21 |
7059.65 |
0.1588 |
| SP23 |
6.86 |
6265.35 |
0.1095 |
68.62 |
11510.74 |
0.5961 |
100.28 |
20064.52 |
0.4998 |
2.53 |
9584.25 |
0.0264 |
32.22 |
3573.51 |
0.9016 |
1.84 |
7753.50 |
0.0237 |
33.31 |
15126.56 |
0.2202 |
42.69 |
7622.22 |
0.5601 |
11.21 |
8763.05 |
0.1279 |
| SP24 |
2.86 |
6318.22 |
0.0453 |
121.06 |
11508.52 |
1.0519 |
133.69 |
16946.16 |
0.7889 |
1.99 |
9477.13 |
0.0210 |
25.81 |
4474.44 |
0.5768 |
2.27 |
7926.91 |
0.0286 |
47.91 |
15776.45 |
0.3037 |
30.02 |
7939.55 |
0.3781 |
10.04 |
8867.42 |
0.1132 |
| SP25 |
43.24 |
5967.19 |
0.7246 |
261.74 |
11024.75 |
2.3741 |
53.23 |
21609.69 |
0.2463 |
16.89 |
9293.80 |
0.1817 |
64.22 |
4222.87 |
1.5208 |
20.92 |
9560.51 |
0.2188 |
162.59 |
18624.39 |
0.8730 |
209.44 |
7299.84 |
2.8691 |
63.50 |
7917.96 |
0.8020 |
| LP1 |
15.93 |
5971.26 |
0.2668 |
66.50 |
12151.90 |
0.5472 |
30.75 |
24958.79 |
0.1232 |
11.80 |
10826.10 |
0.1090 |
35.08 |
4061.19 |
0.8638 |
4.88 |
9319.60 |
0.0524 |
108.30 |
14896.35 |
0.7270 |
47.16 |
8613.79 |
0.5475 |
14.76 |
9583.97 |
0.1540 |
| LP2 |
38.97 |
5921.55 |
0.6581 |
140.81 |
11584.07 |
1.2155 |
63.83 |
21969.13 |
0.2905 |
15.15 |
9814.43 |
0.1544 |
30.41 |
4647.49 |
0.6543 |
2.61 |
8750.97 |
0.0298 |
59.96 |
16728.80 |
0.3584 |
134.72 |
7842.84 |
1.7177 |
30.64 |
6109.14 |
0.5015 |
| LP3 |
10.25 |
6287.19 |
0.1630 |
65.63 |
11013.29 |
0.5959 |
55.16 |
18366.66 |
0.3003 |
2.58 |
10992.07 |
0.0235 |
16.14 |
3302.54 |
0.4887 |
0.65 |
8567.75 |
0.0076 |
28.45 |
14101.74 |
0.2017 |
40.15 |
6593.31 |
0.6090 |
7.10 |
4632.59 |
0.1533 |
| LP4 |
47.76 |
6004.07 |
0.7955 |
180.48 |
11148.05 |
1.6189 |
83.06 |
18093.89 |
0.4590 |
7.65 |
9312.27 |
0.0821 |
48.49 |
3515.42 |
1.3794 |
9.18 |
8187.52 |
0.1121 |
103.28 |
15513.40 |
0.6657 |
94.68 |
8007.43 |
1.1824 |
74.60 |
8640.35 |
0.8634 |
| LP5 |
74.45 |
6011.02 |
1.2386 |
318.14 |
9785.11 |
3.2513 |
50.05 |
16830.48 |
0.2974 |
17.43 |
8490.88 |
0.2053 |
38.14 |
3571.09 |
1.0680 |
11.66 |
7900.11 |
0.1476 |
218.70 |
13106.01 |
1.6687 |
129.60 |
8308.91 |
1.5598 |
44.93 |
5574.15 |
0.8060 |
| LP6 |
22.44 |
6404.35 |
0.3504 |
187.93 |
9320.51 |
2.0163 |
51.11 |
19093.97 |
0.2677 |
10.76 |
10746.47 |
0.1001 |
17.66 |
4145.39 |
0.4260 |
4.29 |
8189.38 |
0.0524 |
137.49 |
13879.01 |
0.9906 |
57.61 |
6486.39 |
0.8882 |
13.94 |
6180.53 |
0.2255 |
| LP7 |
17.40 |
6413.54 |
0.2713 |
167.19 |
10950.61 |
1.5268 |
73.42 |
20616.00 |
0.3561 |
10.75 |
9606.73 |
0.1119 |
20.94 |
3464.43 |
0.6044 |
2.23 |
8717.93 |
0.0256 |
207.21 |
15567.27 |
1.3311 |
51.72 |
8761.56 |
0.5903 |
14.60 |
6213.59 |
0.2350 |
| LP8 |
35.02 |
6285.44 |
0.5572 |
139.27 |
11569.10 |
1.2038 |
72.38 |
18381.09 |
0.3938 |
18.51 |
10138.80 |
0.1826 |
28.90 |
4434.85 |
0.6517 |
7.46 |
9558.72 |
0.0780 |
73.30 |
15357.08 |
0.4773 |
78.65 |
7886.32 |
0.9973 |
24.80 |
8792.99 |
0.2820 |
| LP9 |
41.17 |
5951.60 |
0.6917 |
149.55 |
11075.37 |
1.3503 |
48.55 |
22021.01 |
0.2205 |
9.10 |
11619.61 |
0.0783 |
52.62 |
3058.17 |
1.7206 |
12.36 |
8587.29 |
0.1439 |
182.44 |
15444.00 |
1.1813 |
202.10 |
10583.78 |
1.9095 |
176.76 |
6207.16 |
2.8477 |
| LP10 |
23.74 |
6012.91 |
0.3948 |
187.15 |
10077.16 |
1.8572 |
57.20 |
22847.31 |
0.2504 |
3.20 |
10674.83 |
0.0300 |
11.62 |
3881.87 |
0.2993 |
6.47 |
10514.94 |
0.0615 |
48.04 |
19845.70 |
0.2421 |
111.09 |
8283.89 |
1.3410 |
107.73 |
7557.95 |
1.4254 |
| LP11 |
14.86 |
6110.90 |
0.2432 |
94.14 |
9256.17 |
1.0171 |
16.70 |
26967.52 |
0.0619 |
5.60 |
7780.40 |
0.0720 |
7.52 |
3541.02 |
0.2124 |
3.81 |
7300.09 |
0.0522 |
6.45 |
13678.56 |
0.0472 |
75.66 |
7596.11 |
0.9960 |
46.74 |
6276.56 |
0.7447 |
| LP12 |
8.06 |
6259.52 |
0.1288 |
25.80 |
10729.35 |
0.2405 |
11.61 |
28848.73 |
0.0402 |
1.45 |
9609.21 |
0.0151 |
4.31 |
4095.51 |
0.1052 |
0.93 |
8635.84 |
0.0108 |
2.44 |
11847.35 |
0.0206 |
29.23 |
8736.23 |
0.3346 |
7.00 |
7434.59 |
0.0942 |
| LP13 |
33.36 |
6270.49 |
0.5320 |
274.21 |
10059.65 |
2.7258 |
85.36 |
26195.21 |
0.3259 |
5.22 |
11114.42 |
0.0470 |
44.10 |
3500.71 |
1.2597 |
8.74 |
9771.06 |
0.0894 |
78.86 |
17064.92 |
0.4621 |
148.06 |
8026.46 |
1.8446 |
92.39 |
7807.71 |
1.1833 |
| LP14 |
29.35 |
6070.40 |
0.4835 |
160.58 |
13818.32 |
1.1621 |
57.00 |
27399.97 |
0.2080 |
5.18 |
12682.81 |
0.0408 |
38.41 |
5535.27 |
0.6939 |
9.59 |
11826.29 |
0.0811 |
139.52 |
14718.19 |
0.9479 |
158.90 |
7779.67 |
2.0425 |
85.79 |
7101.48 |
1.2081 |
| LP15 |
14.08 |
6310.67 |
0.2231 |
108.54 |
12226.01 |
0.8878 |
11.43 |
20415.24 |
0.0560 |
4.29 |
10235.21 |
0.0419 |
6.83 |
4786.65 |
0.1427 |
3.49 |
10163.51 |
0.0343 |
4.42 |
16813.25 |
0.0263 |
70.82 |
8454.76 |
0.8376 |
57.87 |
8826.59 |
0.6556 |
| LP16 |
38.10 |
6308.99 |
0.6039 |
224.42 |
10920.13 |
2.0551 |
58.19 |
18915.27 |
0.3076 |
12.98 |
9252.57 |
0.1403 |
25.58 |
3501.54 |
0.7305 |
6.06 |
8361.97 |
0.0725 |
187.80 |
14243.86 |
1.3185 |
79.64 |
8381.28 |
0.9502 |
24.49 |
7349.44 |
0.3332 |
| LA1 |
39.53 |
5844.15 |
0.6764 |
153.40 |
11080.16 |
1.3845 |
53.94 |
17900.19 |
0.3013 |
5.26 |
8940.22 |
0.0588 |
38.98 |
3436.65 |
1.1342 |
10.44 |
7739.17 |
0.1349 |
92.27 |
11396.06 |
0.8097 |
116.20 |
8514.88 |
1.3647 |
16.63 |
8366.72 |
0.1988 |
| 水为 |
7.06 |
6163.58 |
0.1145 |
110.58 |
11359.90 |
0.9734 |
38.25 |
21707.93 |
0.1762 |
8.65 |
9105.55 |
0.0950 |
45.78 |
3419.43 |
1.3388 |
10.95 |
9140.90 |
0.1198 |
84.67 |
16077.78 |
0.5266 |
87.09 |
8669.05 |
1.0046 |
14.22 |
7375.81 |
0.1928 |
| LA3 |
49.51 |
6127.32 |
0.8080 |
189.37 |
10480.08 |
1.8070 |
47.31 |
23969.23 |
0.1974 |
23.46 |
8251.42 |
0.2843 |
73.17 |
3213.54 |
2.2769 |
30.13 |
8053.11 |
0.3741 |
190.71 |
18067.61 |
1.0555 |
206.67 |
10157.90 |
2.0346 |
118.69 |
11012.41 |
1.0778 |
| LA4 |
9.45 |
6231.43 |
0.1517 |
101.15 |
10764.28 |
0.9397 |
43.93 |
18445.78 |
0.2382 |
6.83 |
9106.47 |
0.0750 |
5.48 |
3968.13 |
0.1381 |
2.07 |
7183.48 |
0.0288 |
14.98 |
11095.78 |
0.1350 |
44.31 |
8400.33 |
0.5275 |
18.02 |
5093.33 |
0.3538 |
| LA5 |
8.21 |
6468.09 |
0.1269 |
101.70 |
10782.93 |
0.9432 |
49.41 |
17857.46 |
0.2767 |
4.67 |
9378.50 |
0.0498 |
27.88 |
4320.50 |
0.6453 |
7.67 |
8732.91 |
0.0878 |
245.22 |
11348.94 |
2.1607 |
101.42 |
9642.82 |
1.0518 |
59.10 |
7589.10 |
0.7787 |
| LA6 |
95.03 |
6441.94 |
1.4752 |
53.56 |
10969.89 |
0.4882 |
45.63 |
19113.36 |
0.2387 |
34.05 |
8826.04 |
0.3858 |
22.67 |
3432.16 |
0.6605 |
15.73 |
7873.91 |
0.1998 |
194.49 |
13395.53 |
1.4519 |
203.37 |
9453.82 |
2.1512 |
165.17 |
5220.68 |
3.1638 |
| LA7 |
43.57 |
6390.61 |
0.6818 |
136.48 |
9697.57 |
1.4074 |
44.59 |
17227.16 |
0.2588 |
26.85 |
10617.54 |
0.2529 |
53.25 |
3342.84 |
1.5930 |
8.20 |
8078.65 |
0.1015 |
99.41 |
13308.10 |
0.7470 |
156.91 |
8444.81 |
1.8581 |
124.79 |
6172.25 |
2.0218 |
| LA8 |
25.51 |
5969.99 |
0.4273 |
159.29 |
9551.72 |
1.6677 |
21.69 |
22493.72 |
0.0964 |
7.29 |
12500.69 |
0.0583 |
28.21 |
5068.31 |
0.5566 |
12.52 |
10500.36 |
0.1192 |
2.23 |
19936.15 |
0.0112 |
66.00 |
11282.00 |
0.5850 |
75.19 |
11732.39 |
0.6409 |
| LA9 |
3.26 |
6188.23 |
0.0527 |
19.10 |
14974.86 |
0.1275 |
71.65 |
23929.16 |
0.2994 |
0.84 |
12303.21 |
0.0068 |
3.65 |
4847.99 |
0.0753 |
0.36 |
10895.91 |
0.0033 |
2.47 |
21371.14 |
0.0116 |
18.43 |
10548.00 |
0.1747 |
7.33 |
7698.82 |
0.0952 |
| LA10 |
29.15 |
5884.38 |
0.4954 |
95.43 |
11229.43 |
0.8498 |
89.66 |
20489.06 |
0.4376 |
16.96 |
9051.65 |
0.1874 |
21.61 |
3412.09 |
0.6333 |
16.06 |
8721.17 |
0.1841 |
2.82 |
17303.92 |
0.0163 |
74.14 |
8057.43 |
0.9201 |
27.50 |
5058.68 |
0.5436 |
| LA11 |
10.88 |
6259.53 |
0.1738 |
40.56 |
13131.47 |
0.3089 |
121.99 |
22020.10 |
0.5540 |
2.48 |
10555.63 |
0.0235 |
5.92 |
4847.60 |
0.1221 |
0.48 |
9784.56 |
0.0049 |
7.09 |
16593.97 |
0.0427 |
26.86 |
7655.07 |
0.3509 |
7.73 |
7284.80 |
0.1061 |
| LA12 |
48.02 |
6403.75 |
0.7499 |
149.63 |
9735.88 |
1.5369 |
62.74 |
14671.39 |
0.4276 |
20.20 |
8380.51 |
0.2410 |
56.05 |
3384.94 |
1.6559 |
5.41 |
6896.77 |
0.0784 |
91.38 |
11321.11 |
0.8072 |
109.18 |
5802.35 |
1.8817 |
46.13 |
7209.85 |
0.6398 |
| LA13 |
22.08 |
5983.25 |
0.3690 |
77.34 |
12359.55 |
0.6258 |
49.89 |
24426.48 |
0.2042 |
13.64 |
10650.97 |
0.1281 |
31.43 |
4286.46 |
0.7332 |
3.68 |
8877.22 |
0.0415 |
184.36 |
14885.17 |
1.2385 |
77.19 |
10677.05 |
0.7230 |
23.24 |
10012.96 |
0.2321 |
| LA14 |
29.06 |
6197.60 |
0.4689 |
219.41 |
12097.18 |
1.8137 |
68.78 |
22967.64 |
0.2995 |
7.41 |
10651.10 |
0.0696 |
33.00 |
4079.19 |
0.8090 |
10.50 |
8376.22 |
0.1254 |
21.77 |
16404.09 |
0.1327 |
122.70 |
8502.69 |
1.4431 |
92.02 |
9152.03 |
1.0055 |
| LA15 |
28.90 |
6409.17 |
0.4509 |
11.61 |
12035.67 |
0.0965 |
43.15 |
25371.86 |
0.1701 |
5.48 |
11118.01 |
0.0493 |
35.81 |
3597.27 |
0.9955 |
6.72 |
6464.97 |
0.1039 |
159.51 |
12763.92 |
1.2497 |
120.12 |
7008.78 |
1.7139 |
55.67 |
5676.13 |
0.9808 |
| LA16 |
20.91 |
6243.38 |
0.3349 |
95.37 |
11719.51 |
0.8138 |
41.47 |
21759.15 |
0.1906 |
2.48 |
11669.90 |
0.0213 |
27.93 |
5249.47 |
0.5321 |
5.48 |
10752.39 |
0.0510 |
113.55 |
14828.06 |
0.7658 |
123.52 |
9586.25 |
1.2885 |
34.37 |
10915.62 |
0.3149 |
| LA17 |
33.89 |
6029.51 |
0.5621 |
12.57 |
12627.27 |
0.0995 |
65.10 |
23746.45 |
0.2741 |
13.69 |
10538.29 |
0.1299 |
34.97 |
4468.75 |
0.7825 |
7.61 |
9975.36 |
0.0763 |
117.69 |
13313.53 |
0.8840 |
122.84 |
9248.08 |
1.3283 |
36.44 |
8620.93 |
0.4227 |
| LA18 |
29.56 |
6046.27 |
0.4889 |
137.19 |
10245.05 |
1.3391 |
54.86 |
18325.38 |
0.2994 |
10.74 |
8928.25 |
0.1203 |
48.27 |
3846.06 |
1.2551 |
6.44 |
7467.00 |
0.0862 |
91.91 |
12882.36 |
0.7135 |
125.43 |
7366.73 |
1.7027 |
36.87 |
4820.33 |
0.7649 |
|
|
| 样本 |
瓦尔 |
Cit |
伽马氨基丁酸 |
Cys2 |
箴 |
Trp |
低浓缩铀 |
参数 |
半胱氨酸 |
| 免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
|
| SP1 |
4.42 |
6012.65 |
0.0735 |
2.49 |
4673.88 |
0.0533 |
3.46 |
3353.11 |
0.1032 |
- - - - - - |
11525.08 |
- - - - - - |
4.15 |
6607.74 |
0.0628 |
0.24 |
3637.74 |
0.0066 |
1.37 |
12211.14 |
0.0112 |
34.88 |
9489.73 |
0.3676 |
- - - - - - |
5538.05 |
- - - - - - |
| SP2 |
15.20 |
10648.71 |
0.1427 |
6.07 |
3955.21 |
0.1535 |
21.27 |
4028.12 |
0.5280 |
- - - - - - |
13123.45 |
- - - - - - |
12.90 |
6341.28 |
0.2034 |
0.51 |
6868.79 |
0.0074 |
4.08 |
11230.44 |
0.0363 |
87.38 |
8979.88 |
0.9731 |
- - - - - - |
8675.28 |
- - - - - - |
| SP3 |
15.36 |
6327.29 |
0.2428 |
24.59 |
4218.39 |
0.5829 |
83.54 |
2906.04 |
2.8747 |
0.67 |
9520.68 |
0.0070 |
27.55 |
7911.24 |
0.3482 |
2.67 |
6256.52 |
0.0427 |
33.86 |
9531.17 |
0.3553 |
68.39 |
8667.37 |
0.7891 |
- - - - - - |
3752.42 |
- - - - - - |
| SP4 |
16.09 |
10136.97 |
0.1587 |
9.45 |
4525.17 |
0.2088 |
112.07 |
1860.38 |
6.0240 |
0.71 |
8713.26 |
0.0081 |
22.64 |
9816.87 |
0.2306 |
3.14 |
6886.81 |
0.0456 |
25.37 |
11900.95 |
0.2132 |
41.42 |
7830.13 |
0.5290 |
- - - - - - |
5020.00 |
- - - - - - |
| SP5 |
12.81 |
12270.45 |
0.1044 |
3.68 |
5960.38 |
0.0617 |
9.31 |
2163.00 |
0.4304 |
- - - - - - |
11194.54 |
- - - - - - |
5.92 |
8973.82 |
0.0660 |
0.39 |
6741.47 |
0.0058 |
3.03 |
17710.97 |
0.0171 |
75.96 |
11624.78 |
0.6534 |
- - - - - - |
8152.58 |
- - - - - - |
| SP6 |
18.63 |
6921.90 |
0.2691 |
54.08 |
6475.87 |
0.8351 |
110.08 |
2010.87 |
5.4742 |
0.26 |
9803.15 |
0.0027 |
28.19 |
7126.12 |
0.3956 |
4.73 |
3570.11 |
0.1325 |
26.75 |
15586.82 |
0.1716 |
43.86 |
5689.69 |
0.7709 |
- - - - - - |
3789.97 |
- - - - - - |
| SP7 |
17.07 |
9259.39 |
0.1844 |
29.75 |
4140.35 |
0.7185 |
103.95 |
2825.91 |
3.6785 |
2.41 |
9778.44 |
0.0246 |
24.62 |
8226.72 |
0.2993 |
3.95 |
4856.66 |
0.0813 |
27.66 |
10861.08 |
0.2547 |
34.40 |
4988.56 |
0.6896 |
- - - - - - |
3471.13 |
- - - - - - |
| SP8 |
15.86 |
7296.00 |
0.2174 |
3.13 |
4702.29 |
0.0666 |
20.48 |
1682.03 |
1.2176 |
- - - - - - |
6139.57 |
- - - - - - |
10.52 |
6680.26 |
0.1575 |
0.56 |
4883.67 |
0.0115 |
4.38 |
9640.58 |
0.0454 |
64.28 |
4886.46 |
1.3155 |
- - - - - - |
3120.50 |
- - - - - - |
| SP9 |
37.20 |
6514.46 |
0.5710 |
10.18 |
5254.55 |
0.1937 |
57.02 |
2501.97 |
2.2790 |
0.14 |
10056.41 |
0.0014 |
15.77 |
5340.61 |
0.2953 |
1.20 |
4253.18 |
0.0282 |
14.42 |
11713.99 |
0.1231 |
124.64 |
5090.33 |
2.4486 |
- - - - - - |
3897.56 |
- - - - - - |
| SP10 |
38.29 |
9908.44 |
0.3864 |
13.50 |
7105.23 |
0.1900 |
57.85 |
1953.05 |
2.9620 |
0.26 |
10687.68 |
0.0024 |
19.03 |
7381.05 |
0.2578 |
1.17 |
7036.56 |
0.0166 |
13.77 |
14235.69 |
0.0967 |
108.51 |
4268.65 |
2.5420 |
- - - - - - |
8653.16 |
- - - - - - |
| SP11 |
21.90 |
10089.65 |
0.2171 |
8.30 |
5747.51 |
0.1444 |
108.20 |
3155.71 |
3.4287 |
0.79 |
9550.72 |
0.0083 |
24.66 |
5804.81 |
0.4248 |
1.94 |
7077.68 |
0.0274 |
26.89 |
14118.06 |
0.1905 |
67.72 |
5222.67 |
1.2967 |
- - - - - - |
6165.20 |
- - - - - - |
| SP12 |
36.91 |
5104.20 |
0.7231 |
31.74 |
3267.50 |
0.9714 |
56.19 |
3092.80 |
1.8168 |
0.58 |
8821.94 |
0.0066 |
11.01 |
6511.44 |
0.1691 |
2.35 |
4185.92 |
0.0561 |
10.46 |
8633.77 |
0.1212 |
99.42 |
6433.17 |
1.5454 |
- - - - - - |
2940.84 |
- - - - - - |
| SP13 |
27.76 |
5423.78 |
0.5118 |
27.14 |
3816.49 |
0.7111 |
82.12 |
2484.15 |
3.3058 |
0.99 |
6828.14 |
0.0145 |
22.06 |
7524.55 |
0.2932 |
11.42 |
3149.92 |
0.3625 |
31.25 |
8945.92 |
0.3493 |
73.82 |
5010.51 |
1.4733 |
- - - - - - |
6503.52 |
- - - - - - |
| SP14 |
44.12 |
10896.32 |
0.4049 |
11.57 |
6328.11 |
0.1828 |
70.23 |
4220.23 |
1.6641 |
0.28 |
9165.62 |
0.0031 |
21.63 |
8005.88 |
0.2702 |
2.44 |
4732.57 |
0.0516 |
18.29 |
13035.53 |
0.1403 |
65.62 |
6826.41 |
0.9613 |
- - - - - - |
8677.59 |
- - - - - - |
| SP15 |
6.17 |
7444.35 |
0.0829 |
5.45 |
3756.96 |
0.1451 |
4.23 |
3324.82 |
0.1272 |
- - - - - - |
10198.26 |
- - - - - - |
14.42 |
5416.47 |
0.2662 |
- - - - - - |
5143.26 |
- - - - - - |
1.80 |
14814.40 |
0.0122 |
30.19 |
5059.02 |
0.5968 |
- - - - - - |
6694.08 |
- - - - - - |
| SP16 |
26.04 |
6396.60 |
0.4071 |
36.82 |
3762.26 |
0.9787 |
134.46 |
3870.97 |
3.4735 |
0.72 |
8704.16 |
0.0083 |
27.10 |
7353.81 |
0.3685 |
15.39 |
7545.48 |
0.2040 |
30.79 |
12588.03 |
0.2446 |
43.32 |
6483.46 |
0.6682 |
- - - - - - |
4369.57 |
- - - - - - |
| SP17 |
9.24 |
8969.61 |
0.1030 |
4.12 |
3703.39 |
0.1112 |
2.34 |
3148.92 |
0.0743 |
- - - - - - |
8001.88 |
- - - - - - |
14.78 |
8403.61 |
0.1759 |
- - - - - - |
4014.56 |
- - - - - - |
5.35 |
10274.36 |
0.0521 |
9.96 |
4876.50 |
0.2042 |
- - - - - - |
6360.07 |
- - - - - - |
| SP18 |
26.19 |
12386.71 |
0.2114 |
26.35 |
5209.87 |
0.5058 |
98.88 |
3534.87 |
2.7973 |
0.12 |
12402.18 |
0.0010 |
17.21 |
9211.57 |
0.1868 |
2.08 |
7798.78 |
0.0267 |
22.77 |
17831.20 |
0.1277 |
108.35 |
4662.91 |
2.3237 |
- - - - - - |
7665.04 |
- - - - - - |
| SP19 |
25.16 |
8312.52 |
0.3027 |
8.51 |
4849.89 |
0.1755 |
73.42 |
1868.93 |
3.9285 |
0.28 |
8968.53 |
0.0031 |
17.64 |
8754.89 |
0.2015 |
3.68 |
5290.70 |
0.0696 |
16.70 |
11607.35 |
0.1439 |
80.47 |
7406.94 |
1.0864 |
- - - - - - |
7063.71 |
- - - - - - |
| SP20 |
25.85 |
8395.03 |
0.3079 |
17.22 |
4586.93 |
0.3754 |
131.28 |
2873.62 |
4.5685 |
0.40 |
8894.24 |
0.0045 |
18.50 |
4472.47 |
0.4136 |
9.86 |
3888.62 |
0.2536 |
24.40 |
8829.37 |
0.2764 |
42.54 |
4662.51 |
0.9124 |
- - - - - - |
4657.46 |
- - - - - - |
| SP21 |
11.27 |
5982.04 |
0.1884 |
24.90 |
4366.50 |
0.5703 |
10.82 |
3551.90 |
0.3046 |
0.24 |
8933.72 |
0.0027 |
28.86 |
6002.68 |
0.4808 |
0.15 |
6460.55 |
0.0023 |
3.89 |
12300.36 |
0.0316 |
55.98 |
5963.67 |
0.9387 |
- - - - - - |
5955.22 |
- - - - - - |
| SP22 |
10.06 |
9469.47 |
0.1062 |
5.09 |
5545.62 |
0.0918 |
1.90 |
1902.37 |
0.0999 |
- - - - - - |
10023.81 |
- - - - - - |
18.22 |
5611.46 |
0.3247 |
0.14 |
3261.78 |
0.0043 |
9.76 |
12591.58 |
0.0775 |
18.52 |
8217.10 |
0.2254 |
- - - - - - |
6884.34 |
- - - - - - |
| SP23 |
19.80 |
6941.68 |
0.2852 |
35.46 |
4560.09 |
0.7776 |
10.96 |
2519.87 |
0.4349 |
0.31 |
11078.52 |
0.0028 |
25.31 |
7474.12 |
0.3386 |
0.55 |
4776.94 |
0.0115 |
5.34 |
9323.61 |
0.0573 |
75.70 |
8379.33 |
0.9034 |
- - - - - - |
4296.33 |
- - - - - - |
| SP24 |
24.68 |
7991.34 |
0.3088 |
29.19 |
6319.28 |
0.4619 |
4.94 |
3668.10 |
0.1347 |
0.09 |
10178.72 |
0.0009 |
23.29 |
5293.61 |
0.4400 |
0.60 |
4551.39 |
0.0132 |
3.83 |
14205.93 |
0.0270 |
58.04 |
6170.55 |
0.9406 |
- - - - - - |
5644.77 |
- - - - - - |
| SP25 |
28.45 |
7459.65 |
0.3814 |
46.15 |
5088.78 |
0.9069 |
163.48 |
3897.18 |
4.1948 |
1.16 |
11215.85 |
0.0103 |
24.67 |
7584.57 |
0.3253 |
7.63 |
7080.31 |
0.1078 |
35.40 |
14162.74 |
0.2500 |
74.34 |
9656.72 |
0.7698 |
- - - - - - |
6166.09 |
- - - - - - |
| LP1 |
21.36 |
7244.74 |
0.2948 |
5.16 |
4405.39 |
0.1171 |
58.37 |
2407.83 |
2.4242 |
0.13 |
13065.90 |
0.0010 |
11.76 |
8651.28 |
0.1359 |
3.62 |
5123.83 |
0.0707 |
13.93 |
10764.87 |
0.1294 |
30.24 |
8884.37 |
0.3404 |
- - - - - - |
4324.94 |
- - - - - - |
| LP2 |
14.46 |
7373.76 |
0.1961 |
16.36 |
4874.65 |
0.3356 |
40.10 |
2138.03 |
1.8756 |
0.77 |
7988.91 |
0.0096 |
14.86 |
8851.40 |
0.1679 |
14.60 |
3817.53 |
0.3824 |
15.17 |
13357.73 |
0.1136 |
26.71 |
9701.11 |
0.2753 |
- - - - - - |
7977.29 |
- - - - - - |
| LP3 |
9.83 |
8734.76 |
0.1125 |
60.38 |
3744.80 |
1.6124 |
14.14 |
2099.63 |
0.6735 |
- - - - - - |
9725.45 |
- - - - - - |
18.43 |
4540.45 |
0.4059 |
0.45 |
4728.65 |
0.0095 |
3.52 |
9822.04 |
0.0358 |
74.16 |
5810.06 |
1.2764 |
- - - - - - |
3530.69 |
- - - - - - |
| LP4 |
35.80 |
9919.45 |
0.3609 |
3.79 |
3526.80 |
0.1075 |
122.04 |
2438.49 |
5.0047 |
0.37 |
9387.49 |
0.0039 |
18.34 |
8296.58 |
0.2211 |
1.14 |
5349.26 |
0.0213 |
22.21 |
9157.07 |
0.2425 |
48.84 |
8460.67 |
0.5773 |
- - - - - - |
6861.56 |
- - - - - - |
| LP5 |
35.99 |
8871.65 |
0.4057 |
11.90 |
4587.62 |
0.2594 |
133.05 |
3159.85 |
4.2106 |
4.21 |
7298.47 |
0.0577 |
25.23 |
5061.04 |
0.4985 |
4.34 |
5510.02 |
0.0788 |
31.89 |
11224.14 |
0.2841 |
45.88 |
6939.12 |
0.6612 |
- - - - - - |
3854.06 |
- - - - - - |
| LP6 |
24.78 |
7215.09 |
0.3434 |
9.73 |
4416.05 |
0.2203 |
91.48 |
2092.89 |
4.3710 |
2.32 |
7641.78 |
0.0304 |
14.71 |
6655.83 |
0.2210 |
5.76 |
3078.31 |
0.1871 |
13.10 |
10225.91 |
0.1281 |
77.03 |
6842.51 |
1.1258 |
- - - - - - |
3272.62 |
- - - - - - |
| LP7 |
18.41 |
6396.21 |
0.2878 |
10.84 |
4275.51 |
0.2535 |
64.21 |
2197.40 |
2.9221 |
4.25 |
11232.05 |
0.0378 |
9.15 |
8097.10 |
0.1130 |
4.71 |
3289.73 |
0.1432 |
11.93 |
9991.64 |
0.1194 |
30.66 |
7921.66 |
0.3870 |
0.14 |
6607.63 |
0.0021 |
| LP8 |
23.88 |
7401.70 |
0.3226 |
16.38 |
4758.26 |
0.3442 |
69.10 |
3565.33 |
1.9381 |
0.55 |
8739.92 |
0.0063 |
14.73 |
8982.82 |
0.1640 |
2.82 |
4541.13 |
0.0621 |
24.29 |
16149.82 |
0.1504 |
31.42 |
6643.33 |
0.4730 |
- - - - - - |
5673.14 |
- - - - - - |
| LP9 |
22.74 |
8090.74 |
0.2811 |
32.17 |
6784.16 |
0.4742 |
95.67 |
3874.88 |
2.4690 |
0.34 |
11756.40 |
0.0029 |
25.48 |
9895.35 |
0.2575 |
3.59 |
4755.18 |
0.0755 |
30.55 |
11213.15 |
0.2724 |
118.53 |
7876.17 |
1.5049 |
0.22 |
4743.85 |
0.0046 |
| LP10 |
44.70 |
10435.87 |
0.4283 |
15.47 |
3862.27 |
0.4005 |
82.77 |
2085.51 |
3.9688 |
0.95 |
13573.29 |
0.0070 |
21.56 |
8888.77 |
0.2426 |
0.77 |
7378.31 |
0.0104 |
16.31 |
14411.77 |
0.1132 |
63.57 |
9539.07 |
0.6664 |
0.14 |
8866.38 |
0.0016 |
| LP11 |
30.18 |
7118.23 |
0.4240 |
10.12 |
3840.01 |
0.2635 |
38.62 |
2188.18 |
1.7649 |
0.39 |
9579.85 |
0.0041 |
14.31 |
6477.73 |
0.2209 |
0.56 |
4389.22 |
0.0128 |
9.34 |
10905.75 |
0.0856 |
74.49 |
5696.30 |
1.3077 |
- - - - - - |
5334.87 |
- - - - - - |
| LP12 |
10.67 |
9073.21 |
0.1176 |
9.45 |
4795.94 |
0.1970 |
6.74 |
2798.07 |
0.2409 |
- - - - - - |
9754.10 |
- - - - - - |
10.14 |
5687.18 |
0.1783 |
0.22 |
6220.07 |
0.0035 |
2.99 |
11120.12 |
0.0269 |
89.48 |
9822.46 |
0.9110 |
- - - - - - |
7787.85 |
- - - - - - |
| LP13 |
24.43 |
7986.97 |
0.3059 |
10.20 |
5208.39 |
0.1958 |
105.99 |
3587.82 |
2.9542 |
1.19 |
10899.43 |
0.0109 |
25.32 |
9578.40 |
0.2643 |
2.01 |
6255.14 |
0.0321 |
24.85 |
10572.72 |
0.2350 |
76.46 |
7230.87 |
1.0574 |
- - - - - - |
8350.79 |
- - - - - - |
| LP14 |
21.85 |
12895.61 |
0.1694 |
10.61 |
6247.35 |
0.1698 |
114.88 |
3385.86 |
3.3929 |
0.65 |
12941.50 |
0.0050 |
24.21 |
10165.28 |
0.2382 |
0.42 |
5801.66 |
0.0072 |
24.35 |
12767.46 |
0.1907 |
38.76 |
9267.96 |
0.4182 |
0.10 |
4749.52 |
0.0021 |
| LP15 |
30.04 |
10600.31 |
0.2834 |
8.02 |
6112.10 |
0.1312 |
39.61 |
2063.22 |
1.9198 |
0.26 |
10874.29 |
0.0024 |
14.42 |
6109.32 |
0.2360 |
0.63 |
7137.55 |
0.0088 |
7.68 |
12394.41 |
0.0620 |
71.29 |
6514.00 |
1.0944 |
- - - - - - |
4351.01 |
- - - - - - |
| LP16 |
26.39 |
5728.98 |
0.4606 |
10.83 |
4203.58 |
0.2576 |
96.25 |
3176.63 |
3.0299 |
3.59 |
6782.15 |
0.0529 |
16.36 |
8733.17 |
0.1873 |
4.94 |
6147.21 |
0.0804 |
18.97 |
9376.15 |
0.2023 |
51.19 |
8498.90 |
0.6023 |
0.14 |
5213.83 |
0.0027 |
| LA1 |
45.00 |
5702.67 |
0.7891 |
13.87 |
3437.80 |
0.4035 |
97.36 |
3259.71 |
2.9868 |
0.34 |
9934.14 |
0.0034 |
16.88 |
5375.18 |
0.3140 |
5.39 |
5649.12 |
0.0954 |
25.80 |
11813.05 |
0.2184 |
46.47 |
7148.37 |
0.6501 |
- - - - - - |
6609.48 |
- - - - - - |
| 水为 |
19.62 |
10334.80 |
0.1898 |
28.58 |
3747.09 |
0.7627 |
81.64 |
2390.45 |
3.4153 |
0.86 |
7845.80 |
0.0110 |
12.38 |
8095.98 |
0.1529 |
2.57 |
5265.52 |
0.0488 |
23.74 |
15177.45 |
0.1564 |
33.03 |
7150.11 |
0.4620 |
- - - - - - |
5064.86 |
- - - - - - |
| LA3 |
39.08 |
11531.59 |
0.3389 |
11.17 |
7658.73 |
0.1458 |
96.78 |
3420.33 |
2.8296 |
0.34 |
9648.03 |
0.0035 |
25.22 |
6740.71 |
0.3741 |
14.39 |
6990.99 |
0.2058 |
28.29 |
16947.54 |
0.1669 |
35.87 |
11157.91 |
0.3215 |
0.12 |
5317.49 |
0.0023 |
| LA4 |
30.49 |
9094.55 |
0.3353 |
5.75 |
3337.54 |
0.1723 |
59.10 |
1960.88 |
3.0140 |
- - - - - - |
7030.26 |
- - - - - - |
21.76 |
7834.00 |
0.2778 |
1.38 |
6225.95 |
0.0222 |
12.30 |
8279.16 |
0.1486 |
67.99 |
6933.19 |
0.9806 |
- - - - - - |
5458.32 |
- - - - - - |
| LA5 |
41.41 |
10636.04 |
0.3893 |
53.62 |
5018.49 |
1.0684 |
47.11 |
3686.56 |
1.2779 |
1.53 |
7468.47 |
0.0205 |
28.74 |
6068.81 |
0.4736 |
1.29 |
4893.15 |
0.0264 |
22.91 |
11517.82 |
0.1989 |
104.70 |
5679.74 |
1.8434 |
- - - - - - |
6326.99 |
- - - - - - |
| LA6 |
26.86 |
10152.01 |
0.2646 |
10.02 |
5879.06 |
0.1704 |
109.90 |
3477.43 |
3.1604 |
0.66 |
10000.06 |
0.0066 |
23.06 |
5135.21 |
0.4491 |
7.12 |
3736.03 |
0.1906 |
33.26 |
15229.07 |
0.2184 |
38.54 |
6481.81 |
0.5946 |
0.24 |
5289.57 |
0.0045 |
| LA7 |
37.39 |
5299.21 |
0.7056 |
7.21 |
4582.47 |
0.1573 |
137.64 |
3164.85 |
4.3490 |
- - - - - - |
6820.33 |
- - - - - - |
25.78 |
4508.55 |
0.5718 |
2.81 |
5062.03 |
0.0555 |
30.16 |
14035.50 |
0.2149 |
37.11 |
8557.89 |
0.4336 |
- - - - - - |
6719.24 |
- - - - - - |
| LA8 |
33.07 |
10470.63 |
0.3158 |
14.37 |
6157.76 |
0.2334 |
26.46 |
2514.35 |
1.0524 |
- - - - - - |
12359.55 |
- - - - - - |
27.27 |
8147.42 |
0.3347 |
3.27 |
7846.61 |
0.0417 |
18.76 |
12842.68 |
0.1461 |
88.93 |
8060.89 |
1.1032 |
- - - - - - |
4516.77 |
- - - - - - |
| LA9 |
3.40 |
12345.60 |
0.0275 |
9.98 |
5675.92 |
0.1758 |
2.04 |
4742.21 |
0.0430 |
- - - - - - |
15127.20 |
- - - - - - |
6.76 |
10585.00 |
0.0639 |
0.29 |
8961.94 |
0.0032 |
1.34 |
12900.12 |
0.0104 |
13.42 |
10716.38 |
0.1252 |
- - - - - - |
5820.67 |
- - - - - - |
| LA10 |
32.37 |
8506.23 |
0.3805 |
15.12 |
4869.46 |
0.3105 |
40.05 |
3515.47 |
1.1393 |
0.11 |
11225.08 |
0.0010 |
15.86 |
4962.73 |
0.3196 |
1.40 |
6261.69 |
0.0224 |
8.88 |
9143.75 |
0.0971 |
86.86 |
5400.65 |
1.6083 |
- - - - - - |
7815.34 |
- - - - - - |
| LA11 |
5.06 |
11187.35 |
0.0452 |
16.69 |
6130.94 |
0.2722 |
2.35 |
2533.92 |
0.0927 |
0.36 |
13012.18 |
0.0028 |
5.43 |
10083.36 |
0.0539 |
0.97 |
5607.42 |
0.0173 |
1.87 |
15543.78 |
0.0120 |
7.40 |
7751.63 |
0.0955 |
- - - - - - |
4413.68 |
- - - - - - |
| LA12 |
38.36 |
6567.71 |
0.5841 |
37.15 |
4331.21 |
0.8577 |
73.93 |
1638.83 |
4.5111 |
0.13 |
10001.78 |
0.0013 |
19.64 |
5219.17 |
0.3763 |
1.98 |
3334.61 |
0.0594 |
16.66 |
13924.60 |
0.1196 |
43.90 |
4477.46 |
0.9805 |
- - - - - - |
3708.31 |
- - - - - - |
| LA13 |
33.49 |
9334.49 |
0.3588 |
21.25 |
5427.96 |
0.3915 |
57.89 |
3900.56 |
1.4841 |
0.15 |
11927.09 |
0.0013 |
18.94 |
7570.98 |
0.2502 |
1.52 |
3832.52 |
0.0397 |
14.66 |
14801.24 |
0.0990 |
130.27 |
9104.06 |
1.4309 |
- - - - - - |
4437.40 |
- - - - - - |
| LA14 |
48.22 |
9862.78 |
0.4889 |
10.25 |
5078.48 |
0.2018 |
68.84 |
1820.89 |
3.7806 |
0.18 |
12167.89 |
0.0015 |
20.49 |
9331.57 |
0.2196 |
3.12 |
6487.16 |
0.0481 |
27.88 |
15343.15 |
0.1817 |
73.66 |
5754.34 |
1.2801 |
- - - - - - |
4845.05 |
- - - - - - |
| LA15 |
33.62 |
4870.39 |
0.6903 |
38.52 |
4318.57 |
0.8920 |
46.08 |
2538.52 |
1.8152 |
- - - - - - |
8893.47 |
- - - - - - |
18.06 |
5895.62 |
0.3063 |
0.58 |
3366.22 |
0.0172 |
18.67 |
7744.33 |
0.2411 |
97.99 |
6807.78 |
1.4394 |
0.19 |
6361.27 |
0.0030 |
| LA16 |
28.73 |
10676.60 |
0.2691 |
17.16 |
5956.75 |
0.2881 |
30.34 |
4234.41 |
0.7165 |
0.10 |
10936.85 |
0.0009 |
20.90 |
7579.89 |
0.2757 |
1.06 |
6621.76 |
0.0160 |
17.03 |
15351.32 |
0.1109 |
39.65 |
7199.84 |
0.5507 |
0.16 |
4666.63 |
0.0034 |
| LA17 |
25.33 |
11534.16 |
0.2196 |
8.43 |
5093.69 |
0.1655 |
57.74 |
3692.35 |
1.5638 |
0.41 |
10297.60 |
0.0040 |
12.50 |
7491.18 |
0.1669 |
4.43 |
3719.53 |
0.1191 |
16.02 |
17696.48 |
0.0905 |
32.56 |
9782.67 |
0.3328 |
- - - - - - |
8946.44 |
- - - - - - |
| LA18 |
24.44 |
9269.41 |
0.2637 |
7.67 |
3801.25 |
0.2018 |
56.82 |
3179.11 |
1.7873 |
0.60 |
9490.75 |
0.0063 |
15.74 |
6342.64 |
0.2482 |
2.92 |
3811.54 |
0.0766 |
15.43 |
11215.99 |
0.1376 |
37.22 |
8358.76 |
0.4453 |
- - - - - - |
3946.18 |
- - - - - - |
|
|
| 样本 |
Hpro |
见过 |
用力推 |
Asn |
Gln |
爵士 |
总游离氨基酸 |
总蛋白(%) |
|
|
|
|
|
|
|
| 免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
免费的 |
总 |
比 |
|
| SP1 |
0.34 |
12275.50 |
0.0028 |
- - - - - - |
2102.26 |
- - - - - - |
12.78 |
7963.72 |
0.1605 |
39.82 |
3834.80 |
1.0384 |
1.04 |
1971.45 |
0.0528 |
4.76 |
6466.09 |
0.0736 |
172.07 |
16.19 |
|
|
|
|
|
|
|
| SP2 |
0.84 |
11585.53 |
0.0073 |
- - - - - - |
1927.32 |
- - - - - - |
38.01 |
7239.98 |
0.5250 |
215.37 |
4986.79 |
4.3188 |
3.20 |
2096.01 |
0.1527 |
22.43 |
5929.40 |
0.3783 |
646.85 |
18.88 |
|
|
|
|
|
|
|
| SP3 |
10.37 |
9975.53 |
0.1040 |
4.20 |
1384.33 |
0.3034 |
157.92 |
4945.98 |
3.1929 |
133.70 |
3711.29 |
3.6025 |
68.98 |
1951.23 |
3.5352 |
144.02 |
5954.58 |
2.4186 |
1437.51 |
15.19 |
|
|
|
|
|
|
|
| SP4 |
3.50 |
12741.33 |
0.0275 |
- - - - - - |
1958.62 |
- - - - - - |
168.24 |
8709.13 |
1.9318 |
139.13 |
4253.91 |
3.2706 |
143.70 |
2036.62 |
7.0558 |
163.42 |
5609.49 |
2.9133 |
1520.16 |
17.82 |
|
|
|
|
|
|
|
| SP5 |
0.48 |
15829.31 |
0.0030 |
- - - - - - |
2151.19 |
- - - - - - |
34.91 |
8355.57 |
0.4178 |
258.84 |
4912.45 |
5.2691 |
3.06 |
2256.83 |
0.1356 |
16.69 |
10234.90 |
0.1631 |
546.01 |
20.46 |
|
|
|
|
|
|
|
| SP6 |
6.53 |
12371.35 |
0.0528 |
- - - - - - |
1608.64 |
- - - - - - |
185.93 |
5726.31 |
3.2469 |
131.14 |
4602.59 |
2.8493 |
53.20 |
2164.53 |
2.4578 |
144.62 |
8041.81 |
1.7984 |
1568.88 |
17.51 |
|
|
|
|
|
|
|
| SP7 |
3.37 |
12423.61 |
0.0271 |
0.10 |
1470.47 |
0.0068 |
168.48 |
4848.42 |
3.4749 |
137.82 |
3978.83 |
3.4638 |
115.64 |
2145.57 |
5.3897 |
153.92 |
8601.82 |
1.7894 |
1616.80 |
15.59 |
|
|
|
|
|
|
|
| SP8 |
0.83 |
9760.19 |
0.0085 |
- - - - - - |
1320.98 |
- - - - - - |
36.00 |
5251.98 |
0.6855 |
243.18 |
3721.89 |
6.5338 |
4.33 |
2074.31 |
0.2087 |
23.02 |
7285.14 |
0.3160 |
601.76 |
14.41 |
|
|
|
|
|
|
|
| SP9 |
2.33 |
10641.34 |
0.0219 |
0.10 |
1927.64 |
0.0052 |
101.08 |
6224.18 |
1.6240 |
287.73 |
3643.59 |
7.8969 |
22.11 |
2123.14 |
1.0414 |
80.72 |
7300.64 |
1.1057 |
1176.53 |
14.43 |
|
|
|
|
|
|
|
| SP10 |
5.44 |
15070.02 |
0.0361 |
0.26 |
2233.47 |
0.0116 |
111.26 |
9030.91 |
1.2320 |
295.67 |
4158.01 |
7.1109 |
19.66 |
2820.23 |
0.6971 |
98.55 |
9877.31 |
0.9977 |
1162.05 |
19.14 |
|
|
|
|
|
|
|
| SP11 |
11.23 |
13641.99 |
0.0823 |
0.28 |
1744.92 |
0.0160 |
176.59 |
7985.90 |
2.2113 |
140.18 |
4655.28 |
3.0112 |
144.55 |
2112.77 |
6.8417 |
179.12 |
6088.37 |
2.9420 |
1570.89 |
17.01 |
|
|
|
|
|
|
|
| SP12 |
12.01 |
11162.90 |
0.1076 |
0.24 |
1881.32 |
0.0128 |
125.79 |
4403.32 |
2.8567 |
137.25 |
3369.96 |
4.0727 |
41.76 |
1600.68 |
2.6089 |
57.70 |
6605.01 |
0.8736 |
1145.82 |
14.13 |
|
|
|
|
|
|
|
| SP13 |
2.39 |
10615.66 |
0.0225 |
3.51 |
1382.26 |
0.2539 |
228.15 |
6391.62 |
3.5695 |
165.25 |
4095.51 |
4.0349 |
269.51 |
1696.44 |
15.8868 |
168.80 |
6708.25 |
2.5163 |
1755.14 |
13.75 |
|
|
|
|
|
|
|
| SP14 |
1.44 |
13205.06 |
0.0109 |
- - - - - - |
2393.23 |
- - - - - - |
132.66 |
8976.95 |
1.4778 |
149.56 |
4798.05 |
3.1171 |
125.79 |
2465.90 |
5.1012 |
129.14 |
7298.41 |
1.7694 |
1255.50 |
19.06 |
|
|
|
|
|
|
|
| SP15 |
6.10 |
10366.51 |
0.0588 |
- - - - - - |
1814.22 |
- - - - - - |
15.94 |
7685.12 |
0.2074 |
22.02 |
4676.65 |
0.4708 |
6.45 |
2382.45 |
0.2707 |
7.22 |
5548.49 |
0.1301 |
194.11 |
15.85 |
|
|
|
|
|
|
|
| SP16 |
7.67 |
12903.86 |
0.0594 |
0.23 |
2509.21 |
0.0092 |
85.06 |
5550.87 |
1.5324 |
142.06 |
4329.68 |
3.2811 |
176.62 |
3056.34 |
5.7788 |
145.74 |
5866.53 |
2.4843 |
1678.57 |
18.29 |
|
|
|
|
|
|
|
| SP17 |
2.03 |
10626.57 |
0.0191 |
- - - - - - |
1794.68 |
- - - - - - |
10.28 |
5329.55 |
0.1929 |
3.24 |
3731.24 |
0.0868 |
2.10 |
2828.19 |
0.0743 |
6.37 |
4657.92 |
0.1368 |
166.05 |
15.26 |
|
|
|
|
|
|
|
| SP18 |
3.88 |
12874.45 |
0.0301 |
0.25 |
2623.84 |
0.0095 |
124.83 |
7649.77 |
1.6318 |
148.44 |
4745.86 |
3.1278 |
123.11 |
3038.81 |
4.0513 |
102.24 |
8464.54 |
1.2079 |
1304.51 |
19.91 |
|
|
|
|
|
|
|
| SP19 |
6.64 |
13923.42 |
0.0477 |
0.39 |
1611.21 |
0.0242 |
116.76 |
6748.38 |
1.7302 |
49.92 |
4539.22 |
1.0997 |
45.19 |
3232.29 |
1.3981 |
77.95 |
5525.06 |
1.4108 |
1063.99 |
17.50 |
|
|
|
|
|
|
|
| SP20 |
1.26 |
8623.89 |
0.0146 |
0.17 |
1655.87 |
0.0103 |
184.54 |
4751.82 |
3.8836 |
152.52 |
3938.78 |
3.8723 |
120.92 |
2150.51 |
5.6229 |
176.26 |
7450.83 |
2.3656 |
1670.95 |
13.61 |
|
|
|
|
|
|
|
| SP21 |
10.27 |
10862.57 |
0.0945 |
- - - - - - |
1789.85 |
- - - - - - |
19.55 |
7093.47 |
0.2756 |
38.03 |
4126.30 |
0.9216 |
7.72 |
2908.79 |
0.2654 |
11.94 |
6041.00 |
0.1976 |
460.11 |
16.55 |
|
|
|
|
|
|
|
| SP22 |
3.58 |
11476.96 |
0.0312 |
0.36 |
1441.11 |
0.0250 |
11.15 |
5896.68 |
0.1891 |
3.63 |
3801.35 |
0.0955 |
8.88 |
2075.29 |
0.4279 |
6.66 |
7009.63 |
0.0950 |
192.55 |
15.30 |
|
|
|
|
|
|
|
| SP23 |
6.80 |
11931.55 |
0.0570 |
- - - - - - |
1557.75 |
- - - - - - |
48.22 |
5634.77 |
0.8558 |
90.90 |
3869.18 |
2.3493 |
14.04 |
1927.16 |
0.7285 |
18.66 |
8290.65 |
0.2251 |
651.61 |
16.73 |
|
|
|
|
|
|
|
| SP24 |
6.27 |
10593.16 |
0.0592 |
0.12 |
2127.69 |
0.0056 |
64.57 |
5101.21 |
1.2658 |
104.24 |
3837.96 |
2.7160 |
14.64 |
2823.06 |
0.5186 |
24.13 |
9012.82 |
0.2677 |
734.27 |
16.87 |
|
|
|
|
|
|
|
| SP25 |
1.86 |
13824.44 |
0.0135 |
0.44 |
2716.54 |
0.0162 |
186.66 |
4842.06 |
3.8550 |
146.65 |
4187.28 |
3.5023 |
97.55 |
2771.87 |
3.5193 |
189.30 |
8595.86 |
2.2022 |
1899.50 |
19.76 |
|
|
|
|
|
|
|
| LP1 |
0.47 |
14631.38 |
0.0032 |
1.18 |
1948.75 |
0.0606 |
65.83 |
6231.55 |
1.0564 |
95.95 |
4013.49 |
2.3907 |
86.11 |
3138.12 |
2.7440 |
85.73 |
5753.40 |
1.4901 |
814.99 |
18.47 |
|
|
|
|
|
|
|
| LP2 |
0.64 |
12206.18 |
0.0052 |
1.85 |
2297.38 |
0.0805 |
78.50 |
5838.37 |
1.3446 |
145.43 |
4899.76 |
2.9681 |
52.18 |
2104.26 |
2.4797 |
73.92 |
6212.65 |
1.1898 |
1012.65 |
17.73 |
|
|
|
|
|
|
|
| LP3 |
4.52 |
8885.71 |
0.0509 |
0.13 |
1858.23 |
0.0070 |
41.09 |
5789.60 |
0.7097 |
124.04 |
2882.55 |
4.3031 |
10.24 |
2456.95 |
0.4168 |
10.18 |
6286.97 |
0.1619 |
597.23 |
13.95 |
|
|
|
|
|
|
|
| LP4 |
5.45 |
10112.04 |
0.0539 |
- - - - - - |
1630.58 |
- - - - - - |
163.52 |
7525.00 |
2.1730 |
143.69 |
3758.46 |
3.8231 |
51.80 |
1875.09 |
2.7625 |
160.80 |
7731.94 |
2.0797 |
1426.96 |
16.05 |
|
|
|
|
|
|
|
| LP5 |
1.71 |
9028.04 |
0.0189 |
- - - - - - |
1621.37 |
- - - - - - |
152.81 |
4615.56 |
3.3108 |
134.30 |
3260.74 |
4.1187 |
112.30 |
1704.64 |
6.5879 |
122.16 |
4651.12 |
2.6265 |
1718.87 |
15.03 |
|
|
|
|
|
|
|
| LP6 |
0.71 |
11053.96 |
0.0064 |
- - - - - - |
1379.86 |
- - - - - - |
85.21 |
6409.94 |
1.3293 |
270.66 |
3875.46 |
6.9839 |
136.75 |
1528.04 |
8.9494 |
72.78 |
6453.61 |
1.1277 |
1308.25 |
13.94 |
|
|
|
|
|
|
|
| LP7 |
0.41 |
10437.44 |
0.0039 |
0.99 |
1616.03 |
0.0613 |
48.37 |
6713.60 |
0.7205 |
199.42 |
3616.48 |
5.5142 |
71.18 |
2426.57 |
2.9334 |
73.61 |
5273.31 |
1.3959 |
1113.74 |
16.11 |
|
|
|
|
|
|
|
| LP8 |
0.60 |
12028.82 |
0.0050 |
2.77 |
2130.37 |
0.1300 |
64.52 |
8264.66 |
0.7807 |
93.71 |
4102.87 |
2.2840 |
128.62 |
3030.50 |
4.2442 |
77.21 |
8764.09 |
0.8810 |
1028.91 |
17.52 |
|
|
|
|
|
|
|
| LP9 |
2.85 |
15395.17 |
0.0185 |
1.09 |
2611.93 |
0.0417 |
95.31 |
6079.19 |
1.5678 |
135.68 |
5800.10 |
2.3393 |
21.02 |
2847.50 |
0.7382 |
160.76 |
6714.80 |
2.3941 |
1620.64 |
20.09 |
|
|
|
|
|
|
|
| LP10 |
3.15 |
12510.19 |
0.0252 |
0.19 |
2294.16 |
0.0083 |
139.99 |
5986.95 |
2.3383 |
316.13 |
4149.54 |
7.6184 |
20.34 |
2755.14 |
0.7383 |
126.41 |
9249.73 |
1.3666 |
1408.67 |
18.88 |
|
|
|
|
|
|
|
| LP11 |
2.02 |
11304.83 |
0.0179 |
0.44 |
1616.02 |
0.0272 |
92.06 |
7087.95 |
1.2988 |
100.62 |
3134.85 |
3.2097 |
9.67 |
2255.64 |
0.4287 |
81.26 |
7479.28 |
1.0865 |
735.57 |
14.11 |
|
|
|
|
|
|
|
| LP12 |
1.26 |
11500.95 |
0.0110 |
0.34 |
1721.63 |
0.0197 |
24.77 |
6419.44 |
0.3859 |
25.52 |
4463.29 |
0.5718 |
3.93 |
2645.29 |
0.1486 |
15.06 |
7598.99 |
0.1982 |
291.40 |
16.46 |
|
|
|
|
|
|
|
| LP13 |
6.41 |
13163.18 |
0.0487 |
0.30 |
2716.29 |
0.0110 |
172.44 |
9432.07 |
1.8282 |
143.41 |
4225.29 |
3.3941 |
134.72 |
2932.22 |
4.5945 |
169.75 |
8497.59 |
1.9976 |
1667.79 |
19.79 |
|
|
|
|
|
|
|
| LP14 |
3.64 |
16225.57 |
0.0224 |
0.17 |
2793.92 |
0.0061 |
175.47 |
6457.65 |
2.7172 |
140.65 |
5052.79 |
2.7836 |
118.28 |
3874.56 |
3.0527 |
171.21 |
9352.55 |
1.8306 |
1529.56 |
21.15 |
|
|
|
|
|
|
|
| LP15 |
1.70 |
11614.17 |
0.0146 |
0.09 |
2501.01 |
0.0036 |
89.92 |
6615.18 |
1.3593 |
269.56 |
3725.94 |
7.2347 |
3.34 |
2877.91 |
0.1161 |
82.11 |
8616.92 |
0.9529 |
900.44 |
18.46 |
|
|
|
|
|
|
|
| LP16 |
0.94 |
11631.20 |
0.0081 |
0.99 |
1651.61 |
0.0599 |
95.46 |
5189.08 |
1.8396 |
201.46 |
3964.71 |
5.0813 |
106.74 |
2083.97 |
5.1220 |
89.52 |
5649.83 |
1.5845 |
1381.04 |
15.81 |
|
|
|
|
|
|
|
| LA1 |
0.75 |
10525.37 |
0.0071 |
0.33 |
1722.41 |
0.0192 |
119.80 |
5747.08 |
2.0845 |
146.08 |
4354.39 |
3.3548 |
117.79 |
2828.86 |
4.1639 |
99.93 |
8354.17 |
1.1962 |
1262.44 |
15.27 |
|
|
|
|
|
|
|
| 水为 |
0.71 |
11050.46 |
0.0064 |
2.14 |
2075.76 |
0.1031 |
88.95 |
5755.14 |
1.5456 |
114.22 |
4714.20 |
2.4229 |
113.30 |
2590.48 |
4.3737 |
128.49 |
8446.54 |
1.5212 |
1057.50 |
16.42 |
|
|
|
|
|
|
|
| LA3 |
1.58 |
14292.88 |
0.0111 |
- - - - - - |
2387.79 |
- - - - - - |
85.96 |
9803.09 |
0.8769 |
129.12 |
5651.48 |
2.2847 |
172.44 |
2107.17 |
8.1835 |
147.16 |
11408.14 |
1.2900 |
1716.54 |
20.72 |
|
|
|
|
|
|
|
| LA4 |
0.78 |
11457.33 |
0.0068 |
- - - - - - |
2109.40 |
- - - - - - |
75.39 |
7598.12 |
0.9922 |
177.20 |
4225.71 |
4.1934 |
34.18 |
2341.13 |
1.4600 |
45.77 |
7073.90 |
0.6470 |
778.32 |
15.58 |
|
|
|
|
|
|
|
| LA5 |
6.18 |
11098.92 |
0.0557 |
0.60 |
1527.50 |
0.0393 |
127.09 |
6426.35 |
1.9776 |
196.17 |
4798.09 |
4.0885 |
29.66 |
2500.29 |
1.1863 |
66.00 |
7750.15 |
0.8516 |
1332.28 |
16.58 |
|
|
|
|
|
|
|
| LA6 |
1.69 |
11256.40 |
0.0150 |
0.14 |
1485.02 |
0.0094 |
92.57 |
6651.83 |
1.3916 |
129.99 |
4328.83 |
3.0029 |
86.79 |
1722.57 |
5.0384 |
157.13 |
8406.98 |
1.8690 |
1547.66 |
16.04 |
|
|
|
|
|
|
|
| LA7 |
2.07 |
11307.16 |
0.0183 |
- - - - - - |
1577.93 |
- - - - - - |
92.43 |
4656.66 |
1.9849 |
145.34 |
4362.67 |
3.3314 |
135.71 |
1877.25 |
7.2292 |
170.21 |
8033.47 |
2.1188 |
1517.92 |
14.71 |
|
|
|
|
|
|
|
| LA8 |
10.79 |
15918.39 |
0.0678 |
0.19 |
2259.56 |
0.0084 |
117.78 |
7883.83 |
1.4939 |
154.15 |
5792.38 |
2.6613 |
216.67 |
2621.75 |
8.2643 |
61.37 |
7941.33 |
0.7728 |
1171.00 |
21.30 |
|
|
|
|
|
|
|
| LA9 |
0.76 |
14178.74 |
0.0054 |
0.14 |
2618.77 |
0.0053 |
6.51 |
6538.81 |
0.0996 |
7.87 |
4624.51 |
0.1702 |
2.68 |
2321.32 |
0.1155 |
6.76 |
10561.97 |
0.0640 |
189.02 |
21.46 |
|
|
|
|
|
|
|
| LA10 |
1.44 |
11088.93 |
0.0130 |
0.26 |
1806.72 |
0.0144 |
62.25 |
6942.43 |
0.8967 |
53.97 |
4802.60 |
1.1238 |
47.46 |
2041.76 |
2.3245 |
36.17 |
5403.37 |
0.6694 |
775.54 |
16.41 |
|
|
|
|
|
|
|
| LA11 |
0.84 |
15220.71 |
0.0055 |
1.65 |
2528.91 |
0.0652 |
8.60 |
7038.75 |
0.1222 |
1.31 |
5236.37 |
0.0250 |
4.91 |
2429.40 |
0.2021 |
7.80 |
6092.24 |
0.1280 |
289.21 |
19.05 |
|
|
|
|
|
|
|
| LA12 |
2.55 |
9164.65 |
0.0278 |
0.18 |
1894.44 |
0.0095 |
174.89 |
4404.00 |
3.9712 |
138.27 |
3675.47 |
3.7620 |
72.48 |
1770.28 |
4.0943 |
85.28 |
5128.63 |
1.6628 |
1294.14 |
14.54 |
|
|
|
|
|
|
|
| LA13 |
2.16 |
13906.13 |
0.0155 |
- - - - - - |
2514.70 |
- - - - - - |
116.52 |
7070.17 |
1.6481 |
294.15 |
3964.62 |
7.4194 |
42.77 |
2727.93 |
1.5679 |
65.75 |
7050.95 |
0.9325 |
1282.38 |
18.31 |
|
|
|
|
|
|
|
| LA14 |
9.08 |
13760.74 |
0.0660 |
0.60 |
2297.56 |
0.0261 |
164.90 |
9014.10 |
1.8294 |
131.85 |
5045.39 |
2.6133 |
63.90 |
2704.59 |
2.3627 |
144.27 |
5601.90 |
2.5754 |
1371.90 |
17.81 |
|
|
|
|
|
|
|
| LA15 |
3.69 |
8467.71 |
0.0436 |
0.30 |
1826.09 |
0.0164 |
135.18 |
5450.03 |
2.4804 |
62.94 |
3497.92 |
1.7994 |
46.02 |
1747.54 |
2.6334 |
51.56 |
4656.58 |
1.1073 |
1020.37 |
13.89 |
|
|
|
|
|
|
|
| LA16 |
2.78 |
16217.53 |
0.0171 |
0.29 |
2603.75 |
0.0111 |
97.73 |
8455.20 |
1.1559 |
22.61 |
5055.17 |
0.4473 |
18.70 |
2206.25 |
0.8476 |
48.71 |
9026.29 |
0.5396 |
811.03 |
20.42 |
|
|
|
|
|
|
|
| LA17 |
0.68 |
12202.21 |
0.0056 |
2.23 |
2011.22 |
0.1109 |
81.41 |
5966.49 |
1.3645 |
207.86 |
3770.32 |
5.5131 |
55.84 |
3327.23 |
1.6783 |
76.89 |
7660.33 |
1.0037 |
1027.14 |
18.44 |
|
|
|
|
|
|
|
| LA18 |
0.55 |
9875.86 |
0.0056 |
2.42 |
1754.15 |
0.1380 |
76.82 |
5468.75 |
1.4047 |
143.75 |
3704.63 |
3.8803 |
52.67 |
1760.52 |
2.9917 |
90.61 |
6192.83 |
1.4631 |
1068.96 |
14.93 |
|
|
|
|
|
|
|
|
|
|
,分析物没有检测到。
|
26矿物质,总蛋白质,水解后的氨基酸含量如表所示5和6。从表可以发现,在59样品中总蛋白含量从13.61%到21.46%不等。氨基酸,如赖氨酸的相关内容,阿拉巴马州,爵士,用力推,GABA, Gln, Asn都略高于其他组件基于每个物种的平均价值在三种不同品种的浮萍。半胱氨酸的内容、Cys2和水解后遇到了更高。显然,它可以看到的内容,自由氨基酸浮萍远低于蛋白结合的氨基酸。此外,数据显示的内容自由氨基酸在浮萍绑定氨基酸呈负相关。
|
| 样本 |
“透明国际” |
V |
Cr |
锰 |
有限公司 |
倪 |
铜 |
锌 |
作为 |
Se |
老 |
莫 |
Cd |
Sn |
某人 |
英航 |
Tl |
Pb |
|
| SP1 |
22.831 |
2.900 |
2.178 |
47.015 |
0.181 |
1.860 |
1.061 |
90.405 |
1.530 |
1.578 |
261.614 |
2.840 |
0.007 |
0.087 |
0.440 |
96.840 |
0.003 |
0.058 |
| SP2 |
27.277 |
1.017 |
2.922 |
36.990 |
0.177 |
1.648 |
0.806 |
80.395 |
2.091 |
1.288 |
199.006 |
3.275 |
0.006 |
0.007 |
0.516 |
60.613 |
0.003 |
0.001 |
| SP3 |
35.621 |
1.915 |
1.838 |
41.295 |
0.162 |
2.899 |
1.500 |
56.437 |
1.127 |
1.983 |
172.960 |
3.017 |
0.016 |
0.168 |
0.386 |
70.196 |
0.002 |
0.024 |
| SP4 |
22.183 |
3.099 |
1.687 |
55.683 |
0.214 |
1.767 |
1.690 |
63.717 |
3.164 |
1.935 |
178.985 |
0.407 |
0.014 |
0.168 |
0.487 |
77.659 |
0.001 |
0.041 |
| SP5 |
45.839 |
3.162 |
1.413 |
24.431 |
0.189 |
2.829 |
0.615 |
71.741 |
0.920 |
1.861 |
275.418 |
2.211 |
0.011 |
0.098 |
0.542 |
63.126 |
0.004 |
0.074 |
| SP6 |
41.446 |
1.783 |
2.371 |
2.110 |
0.240 |
2.157 |
2.805 |
87.665 |
2.146 |
1.464 |
193.120 |
0.577 |
0.002 |
0.121 |
0.536 |
39.704 |
0.004 |
0.046 |
| SP7 |
37.099 |
2.423 |
2.068 |
13.993 |
0.219 |
2.583 |
1.424 |
88.936 |
2.601 |
1.747 |
165.967 |
1.562 |
0.010 |
0.095 |
0.316 |
69.916 |
0.003 |
0.050 |
| SP8 |
28.377 |
2.251 |
2.276 |
54.028 |
0.197 |
1.709 |
2.941 |
46.581 |
2.262 |
2.139 |
249.180 |
2.345 |
0.015 |
0.125 |
0.359 |
110.809 |
0.004 |
0.047 |
| SP9 |
17.084 |
1.069 |
2.452 |
40.559 |
0.192 |
3.140 |
1.247 |
29.974 |
2.770 |
1.664 |
163.694 |
2.659 |
0.015 |
0.139 |
0.637 |
60.895 |
0.004 |
0.018 |
| SP10 |
37.166 |
3.316 |
1.705 |
35.254 |
0.184 |
1.793 |
2.040 |
78.451 |
1.247 |
1.407 |
174.872 |
0.632 |
0.002 |
0.137 |
0.720 |
86.360 |
- - - - - - |
0.003 |
| SP11 |
24.163 |
1.033 |
1.368 |
60.738 |
0.190 |
3.261 |
2.894 |
66.981 |
1.135 |
2.291 |
207.084 |
1.840 |
0.003 |
0.160 |
0.488 |
117.603 |
0.004 |
0.046 |
| SP12 |
38.630 |
2.597 |
1.146 |
53.768 |
0.252 |
2.550 |
1.505 |
76.263 |
1.772 |
2.126 |
180.679 |
0.626 |
0.003 |
0.124 |
0.628 |
142.280 |
0.001 |
0.062 |
| SP13 |
39.728 |
2.347 |
1.577 |
26.733 |
0.173 |
3.173 |
2.882 |
75.098 |
1.628 |
1.112 |
225.261 |
0.323 |
0.012 |
0.106 |
0.550 |
95.230 |
0.001 |
0.001 |
| SP14 |
39.483 |
2.488 |
2.539 |
3.742 |
0.249 |
2.137 |
1.427 |
55.271 |
1.670 |
1.581 |
154.375 |
3.214 |
0.019 |
0.135 |
0.338 |
119.138 |
0.003 |
0.043 |
| SP15 |
19.629 |
2.734 |
2.326 |
22.405 |
0.198 |
3.153 |
1.427 |
55.631 |
1.175 |
2.065 |
247.855 |
2.928 |
0.011 |
0.011 |
0.633 |
136.536 |
0.004 |
0.030 |
| SP16 |
34.619 |
1.068 |
2.064 |
52.250 |
0.163 |
2.169 |
3.017 |
54.488 |
2.321 |
1.985 |
253.553 |
2.155 |
0.002 |
0.120 |
0.377 |
68.487 |
0.001 |
0.022 |
| SP17 |
28.672 |
3.244 |
2.192 |
31.414 |
0.159 |
1.880 |
2.871 |
91.946 |
1.444 |
1.287 |
175.165 |
1.721 |
0.018 |
0.138 |
0.669 |
91.883 |
0.004 |
0.030 |
| SP18 |
25.018 |
1.789 |
2.378 |
45.984 |
0.183 |
2.570 |
2.365 |
24.786 |
1.877 |
2.146 |
194.164 |
3.450 |
0.007 |
0.049 |
0.318 |
95.469 |
0.002 |
0.057 |
| SP19 |
22.802 |
2.136 |
2.199 |
53.284 |
0.212 |
2.704 |
1.054 |
85.613 |
0.913 |
2.073 |
269.684 |
3.174 |
0.014 |
0.043 |
0.423 |
111.149 |
0.003 |
0.004 |
| SP20 |
40.638 |
1.682 |
1.746 |
14.621 |
0.180 |
1.611 |
2.705 |
73.740 |
3.221 |
2.055 |
225.646 |
3.023 |
0.013 |
0.159 |
0.450 |
137.841 |
0.004 |
0.019 |
| SP21 |
23.513 |
1.413 |
2.811 |
12.908 |
0.236 |
1.959 |
0.607 |
22.616 |
2.711 |
1.170 |
227.681 |
3.187 |
0.005 |
0.037 |
0.205 |
62.615 |
0.003 |
0.016 |
| SP22 |
32.010 |
1.755 |
2.461 |
56.750 |
0.202 |
2.515 |
1.114 |
80.116 |
1.210 |
2.322 |
223.754 |
1.075 |
0.015 |
0.155 |
0.573 |
96.469 |
0.002 |
0.012 |
| SP23 |
17.817 |
2.259 |
2.391 |
43.452 |
0.219 |
1.903 |
0.700 |
88.007 |
3.277 |
1.855 |
155.450 |
2.793 |
0.014 |
0.164 |
0.311 |
82.020 |
0.002 |
0.010 |
| SP24 |
24.306 |
2.384 |
1.841 |
32.935 |
0.253 |
1.896 |
3.027 |
63.243 |
1.975 |
1.109 |
214.325 |
2.979 |
0.008 |
0.044 |
0.642 |
47.431 |
0.003 |
0.061 |
| SP25 |
30.842 |
2.878 |
1.693 |
29.377 |
0.254 |
2.786 |
1.811 |
69.276 |
1.082 |
1.349 |
218.438 |
0.896 |
0.011 |
0.125 |
0.474 |
126.036 |
0.001 |
0.032 |
| LP1 |
37.974 |
1.441 |
2.756 |
42.898 |
0.251 |
1.828 |
2.325 |
46.010 |
2.655 |
2.264 |
162.610 |
0.298 |
0.004 |
0.049 |
0.368 |
106.287 |
0.002 |
0.055 |
| LP2 |
25.601 |
3.144 |
1.914 |
10.035 |
0.173 |
2.425 |
2.422 |
53.173 |
1.031 |
1.740 |
211.860 |
0.256 |
0.017 |
0.153 |
0.596 |
73.995 |
0.002 |
0.001 |
| LP3 |
40.576 |
2.424 |
1.579 |
36.158 |
0.203 |
2.635 |
1.373 |
32.781 |
1.839 |
1.363 |
234.793 |
1.450 |
0.001 |
0.145 |
0.697 |
107.956 |
0.001 |
0.032 |
| LP4 |
29.919 |
1.451 |
2.468 |
31.060 |
0.239 |
3.221 |
2.253 |
43.918 |
2.873 |
1.754 |
193.086 |
2.154 |
0.013 |
0.027 |
0.249 |
36.637 |
0.001 |
0.015 |
| LP5 |
43.240 |
2.466 |
2.331 |
46.991 |
0.209 |
3.218 |
1.577 |
26.153 |
1.994 |
2.211 |
234.265 |
1.895 |
0.010 |
0.017 |
0.224 |
84.449 |
0.004 |
0.006 |
| LP6 |
29.925 |
2.796 |
2.933 |
11.764 |
0.248 |
2.974 |
2.174 |
43.896 |
2.394 |
1.784 |
247.178 |
3.487 |
0.012 |
0.017 |
0.336 |
125.785 |
0.003 |
0.016 |
| LP7 |
28.783 |
1.090 |
2.067 |
41.129 |
0.244 |
2.388 |
1.514 |
76.640 |
1.923 |
1.886 |
157.760 |
2.486 |
0.003 |
0.031 |
0.241 |
87.240 |
0.004 |
0.053 |
| LP8 |
45.076 |
3.030 |
1.857 |
7.724 |
0.247 |
2.328 |
2.152 |
87.415 |
2.455 |
2.044 |
225.691 |
2.976 |
0.011 |
0.009 |
0.607 |
111.718 |
0.003 |
0.023 |
| LP9 |
20.651 |
2.339 |
2.731 |
42.403 |
0.257 |
1.681 |
1.012 |
89.960 |
1.066 |
1.855 |
210.521 |
2.237 |
0.007 |
0.052 |
0.424 |
32.520 |
- - - - - - |
0.033 |
| LP10 |
23.769 |
1.646 |
1.288 |
52.927 |
0.243 |
2.319 |
2.950 |
93.752 |
2.929 |
1.882 |
217.655 |
2.496 |
0.017 |
0.165 |
0.196 |
135.624 |
0.003 |
0.051 |
| LP11 |
32.364 |
2.560 |
3.084 |
33.335 |
0.208 |
3.219 |
2.180 |
82.812 |
1.451 |
1.918 |
269.769 |
3.250 |
0.019 |
0.008 |
0.406 |
66.278 |
0.003 |
0.051 |
| LP12 |
24.918 |
1.470 |
2.999 |
37.041 |
0.243 |
1.893 |
1.928 |
95.305 |
2.415 |
1.698 |
224.103 |
1.906 |
0.008 |
0.041 |
0.570 |
64.033 |
0.004 |
0.008 |
| LP13 |
33.468 |
1.704 |
2.451 |
45.575 |
0.176 |
2.507 |
1.882 |
38.837 |
2.524 |
1.580 |
187.087 |
1.245 |
0.003 |
0.133 |
0.285 |
44.075 |
0.003 |
0.030 |
| LP14 |
28.614 |
2.426 |
1.879 |
39.614 |
0.209 |
3.272 |
1.963 |
74.622 |
2.875 |
1.962 |
261.572 |
2.514 |
0.013 |
0.136 |
0.287 |
100.138 |
0.002 |
0.048 |
| LP15 |
35.617 |
2.088 |
2.116 |
42.214 |
0.162 |
2.284 |
1.641 |
39.037 |
1.412 |
1.185 |
244.588 |
0.489 |
0.007 |
0.158 |
0.512 |
110.953 |
0.003 |
0.032 |
| LP16 |
32.522 |
2.845 |
1.545 |
41.087 |
0.186 |
2.100 |
1.860 |
94.105 |
3.060 |
1.921 |
165.053 |
1.398 |
0.015 |
0.008 |
0.451 |
69.796 |
0.002 |
0.066 |
| LA1 |
36.451 |
2.711 |
1.271 |
38.156 |
0.233 |
2.893 |
0.909 |
65.183 |
3.041 |
1.926 |
194.455 |
3.342 |
0.005 |
0.055 |
0.262 |
51.502 |
0.002 |
0.023 |
| 水为 |
43.394 |
2.188 |
2.067 |
17.427 |
0.165 |
2.206 |
1.564 |
58.425 |
3.159 |
1.247 |
254.469 |
1.789 |
0.006 |
0.146 |
0.280 |
33.628 |
0.003 |
0.052 |
| LA3 |
24.311 |
1.120 |
2.917 |
35.712 |
0.247 |
2.359 |
2.087 |
59.228 |
3.345 |
2.092 |
247.432 |
1.778 |
0.010 |
0.108 |
0.362 |
105.945 |
- - - - - - |
0.069 |
| LA4 |
26.794 |
1.110 |
1.710 |
13.192 |
0.232 |
1.909 |
1.963 |
25.002 |
3.351 |
1.618 |
198.614 |
1.801 |
- - - - - - |
0.011 |
0.452 |
33.107 |
0.003 |
0.024 |
| LA5 |
39.248 |
2.722 |
1.585 |
24.608 |
0.203 |
2.755 |
2.186 |
68.836 |
1.896 |
1.634 |
279.990 |
3.413 |
0.016 |
0.037 |
0.213 |
37.247 |
0.004 |
0.077 |
| LA6 |
20.981 |
2.024 |
1.392 |
25.368 |
0.248 |
2.878 |
2.542 |
45.526 |
1.863 |
1.095 |
215.126 |
3.019 |
0.009 |
0.148 |
0.651 |
117.017 |
0.003 |
0.010 |
| LA7 |
30.279 |
1.148 |
2.330 |
62.335 |
0.231 |
2.772 |
2.791 |
44.135 |
2.816 |
1.556 |
187.777 |
3.290 |
0.012 |
0.151 |
0.436 |
36.940 |
0.003 |
0.041 |
| LA8 |
39.053 |
0.971 |
3.084 |
15.630 |
0.243 |
3.097 |
1.738 |
42.571 |
2.567 |
1.411 |
231.825 |
2.281 |
0.010 |
0.013 |
0.499 |
33.670 |
0.003 |
0.053 |
| LA9 |
41.198 |
2.925 |
2.254 |
52.257 |
0.230 |
2.490 |
2.132 |
95.196 |
2.872 |
2.220 |
170.239 |
1.302 |
0.002 |
0.132 |
0.395 |
80.576 |
0.003 |
0.015 |
| LA10 |
16.833 |
1.384 |
2.832 |
34.567 |
0.188 |
2.044 |
1.951 |
62.570 |
0.922 |
1.571 |
169.715 |
0.952 |
0.001 |
0.110 |
0.496 |
81.418 |
0.002 |
0.044 |
| LA11 |
38.564 |
2.900 |
3.171 |
30.344 |
0.257 |
2.860 |
2.083 |
37.280 |
1.155 |
1.073 |
172.207 |
1.959 |
0.008 |
0.117 |
0.433 |
108.524 |
0.002 |
0.014 |
| LA12 |
43.364 |
1.975 |
2.442 |
7.660 |
0.253 |
1.986 |
0.775 |
85.359 |
2.247 |
1.143 |
237.191 |
0.673 |
0.009 |
0.155 |
0.201 |
41.025 |
- - - - - - |
0.002 |
| LA13 |
30.181 |
3.229 |
1.146 |
53.644 |
0.230 |
2.341 |
2.091 |
77.183 |
0.842 |
2.028 |
177.614 |
2.395 |
0.009 |
0.105 |
0.689 |
97.023 |
0.001 |
0.022 |
| LA14 |
31.940 |
3.492 |
1.508 |
64.537 |
0.181 |
2.947 |
2.883 |
24.336 |
3.049 |
2.137 |
243.782 |
1.989 |
0.017 |
0.117 |
0.272 |
125.192 |
0.003 |
0.021 |
| LA15 |
22.170 |
2.327 |
3.077 |
51.812 |
0.175 |
3.050 |
0.998 |
60.176 |
2.860 |
1.716 |
206.597 |
0.281 |
0.006 |
0.058 |
0.397 |
108.215 |
- - - - - - |
0.025 |
| LA16 |
42.638 |
1.570 |
1.978 |
49.228 |
0.258 |
1.959 |
0.924 |
88.563 |
2.498 |
1.683 |
201.422 |
3.242 |
0.014 |
0.029 |
0.295 |
80.373 |
0.003 |
0.075 |
| LA17 |
39.670 |
1.249 |
2.650 |
27.755 |
0.259 |
2.112 |
2.250 |
55.219 |
2.830 |
1.217 |
154.053 |
1.978 |
0.006 |
0.099 |
0.561 |
116.452 |
- - - - - - |
0.047 |
| LA18 |
23.423 |
2.528 |
2.932 |
10.179 |
0.199 |
2.743 |
2.953 |
66.269 |
3.199 |
1.527 |
209.326 |
3.075 |
0.016 |
0.116 |
0.306 |
79.441 |
0.001 |
0.040 |
|
|
| 样本 |
Na |
毫克 |
艾尔 |
K |
Ca |
菲 |
N |
P |
总 |
|
|
|
|
|
|
|
|
|
|
| SP1 |
410579.062 |
209771.718 |
18661.431 |
178472.536 |
591631.101 |
334974.476 |
4.303 |
0.914 |
1744628.970 |
|
|
|
|
|
|
|
|
|
| SP2 |
546214.809 |
232797.639 |
9209.284 |
416708.514 |
386703.829 |
349989.801 |
2.389 |
0.675 |
1942044.979 |
|
|
|
|
|
|
|
|
|
| SP3 |
384732.341 |
302374.639 |
16864.219 |
351597.651 |
645593.344 |
330485.336 |
18.804 |
5.589 |
2032063.470 |
|
|
|
|
|
|
|
|
|
| SP4 |
763978.060 |
217006.139 |
24174.116 |
515345.319 |
525036.011 |
165789.077 |
19.687 |
4.182 |
2211765.493 |
|
|
|
|
|
|
|
|
|
| SP5 |
498927.575 |
304758.187 |
29743.190 |
617594.946 |
663524.773 |
200323.584 |
25.750 |
23.839 |
2315416.327 |
|
|
|
|
|
|
|
|
|
| SP6 |
568688.625 |
219822.814 |
31062.346 |
305039.982 |
486491.766 |
202085.631 |
25.562 |
22.681 |
1813617.704 |
|
|
|
|
|
|
|
|
|
| SP7 |
815621.400 |
203217.299 |
32660.784 |
559562.408 |
566642.730 |
171755.702 |
2.565 |
1.507 |
2349855.405 |
|
|
|
|
|
|
|
|
|
| SP8 |
704283.730 |
188285.004 |
23602.584 |
315772.349 |
624642.806 |
439955.497 |
23.372 |
6.689 |
2297077.677 |
|
|
|
|
|
|
|
|
|
| SP9 |
876213.262 |
151574.474 |
27629.879 |
516294.381 |
409003.501 |
35506.241 |
18.458 |
4.258 |
2016572.669 |
|
|
|
|
|
|
|
|
|
| SP10 |
873643.979 |
294027.085 |
21164.307 |
737471.818 |
383002.078 |
37324.576 |
33.550 |
19.033 |
2347111.719 |
|
|
|
|
|
|
|
|
|
| SP11 |
491391.823 |
256934.016 |
10147.423 |
610308.309 |
636084.667 |
367347.644 |
31.694 |
25.205 |
2372762.062 |
|
|
|
|
|
|
|
|
|
| SP12 |
796497.255 |
269227.175 |
17999.811 |
414658.818 |
512138.780 |
171064.616 |
16.646 |
2.703 |
2182110.814 |
|
|
|
|
|
|
|
|
|
| SP13 |
366808.463 |
156147.673 |
11576.232 |
471660.374 |
756208.109 |
347515.518 |
11.647 |
4.202 |
2110408.155 |
|
|
|
|
|
|
|
|
|
| SP14 |
512150.165 |
243500.636 |
31493.169 |
641312.090 |
499270.353 |
208734.109 |
11.077 |
1.602 |
2136861.051 |
|
|
|
|
|
|
|
|
|
| SP15 |
533557.790 |
280721.134 |
16614.646 |
267728.355 |
378226.451 |
306162.902 |
13.593 |
4.564 |
1783528.184 |
|
|
|
|
|
|
|
|
|
| SP16 |
644403.624 |
276057.049 |
21110.856 |
521532.157 |
583524.045 |
59176.189 |
11.942 |
25.264 |
2106319.987 |
|
|
|
|
|
|
|
|
|
| SP17 |
751229.025 |
173163.933 |
10495.020 |
625004.125 |
472688.229 |
345116.023 |
8.458 |
5.204 |
2378144.753 |
|
|
|
|
|
|
|
|
|
| SP18 |
522140.520 |
176639.957 |
29371.612 |
557932.109 |
690911.336 |
157124.877 |
29.906 |
10.221 |
2134563.150 |
|
|
|
|
|
|
|
|
|
| SP19 |
579415.759 |
238453.094 |
30677.565 |
439717.245 |
686006.551 |
382672.972 |
10.823 |
3.662 |
2357515.153 |
|
|
|
|
|
|
|
|
|
| SP20 |
476281.317 |
182915.790 |
9070.049 |
325811.742 |
373586.252 |
432553.750 |
34.616 |
21.891 |
1800784.763 |
|
|
|
|
|
|
|
|
|
| SP21 |
760676.992 |
188574.381 |
12126.295 |
629919.272 |
484913.883 |
230880.412 |
26.927 |
9.833 |
2307491.687 |
|
|
|
|
|
|
|
|
|
| SP22 |
709408.940 |
239417.173 |
10783.138 |
619462.993 |
550226.213 |
88795.927 |
20.170 |
9.877 |
2218626.941 |
|
|
|
|
|
|
|
|
|
| SP23 |
513303.754 |
213899.504 |
26441.948 |
351694.267 |
732144.084 |
326876.462 |
34.159 |
26.699 |
2164823.520 |
|
|
|
|
|
|
|
|
|
| SP24 |
824465.276 |
238490.190 |
31283.397 |
717257.458 |
679154.325 |
297001.594 |
14.031 |
10.796 |
2788075.528 |
|
|
|
|
|
|
|
|
|
| SP25 |
815267.536 |
215764.623 |
30718.721 |
536395.881 |
508251.297 |
371174.761 |
24.650 |
2.494 |
2478087.325 |
|
|
|
|
|
|
|
|
|
| LP1 |
897950.747 |
158686.979 |
27871.364 |
590366.194 |
696960.175 |
327232.239 |
17.263 |
7.080 |
2699502.116 |
|
|
|
|
|
|
|
|
|
| LP2 |
531566.916 |
294929.433 |
14640.021 |
570417.870 |
648189.841 |
273059.464 |
15.536 |
1.239 |
2333208.859 |
|
|
|
|
|
|
|
|
|
| LP3 |
858626.936 |
266231.149 |
32272.481 |
727712.714 |
480257.660 |
348345.090 |
12.319 |
1.894 |
2713926.251 |
|
|
|
|
|
|
|
|
|
| LP4 |
431098.253 |
221743.162 |
34467.794 |
311381.975 |
785570.359 |
339960.811 |
26.584 |
17.225 |
2124617.501 |
|
|
|
|
|
|
|
|
|
| LP5 |
818404.736 |
237360.411 |
25820.309 |
683695.992 |
566015.946 |
406722.563 |
20.219 |
7.521 |
2738498.958 |
|
|
|
|
|
|
|
|
|
| LP6 |
614726.783 |
203772.151 |
23626.598 |
170198.351 |
715325.262 |
132978.487 |
8.402 |
1.447 |
1861115.202 |
|
|
|
|
|
|
|
|
|
| LP7 |
498622.492 |
200069.361 |
34249.218 |
182114.487 |
450498.128 |
213156.897 |
26.337 |
12.664 |
1579155.065 |
|
|
|
|
|
|
|
|
|
| LP8 |
407734.217 |
308322.609 |
12414.630 |
462642.984 |
726996.054 |
273473.025 |
23.952 |
9.428 |
2192112.264 |
|
|
|
|
|
|
|
|
|
| LP9 |
591923.425 |
196758.141 |
20795.603 |
169080.807 |
379447.395 |
244832.850 |
24.638 |
15.719 |
1603288.325 |
|
|
|
|
|
|
|
|
|
| LP10 |
506188.280 |
278745.249 |
26183.977 |
548614.526 |
619327.368 |
71487.282 |
27.250 |
10.475 |
2051124.319 |
|
|
|
|
|
|
|
|
|
| LP11 |
484360.416 |
252740.828 |
27267.497 |
394747.762 |
420528.173 |
411590.345 |
15.427 |
3.462 |
1991756.825 |
|
|
|
|
|
|
|
|
|
| LP12 |
755545.368 |
290158.455 |
18713.693 |
269669.218 |
653034.467 |
303936.794 |
26.120 |
0.889 |
2291545.587 |
|
|
|
|
|
|
|
|
|
| LP13 |
644431.270 |
171083.444 |
14922.153 |
701260.007 |
799614.153 |
241821.499 |
27.383 |
10.915 |
2573534.387 |
|
|
|
|
|
|
|
|
|
| LP14 |
855511.785 |
226105.300 |
11812.507 |
382201.190 |
657443.790 |
421778.147 |
4.108 |
0.257 |
2555378.850 |
|
|
|
|
|
|
|
|
|
| LP15 |
840333.425 |
261183.216 |
19461.126 |
468066.062 |
568653.040 |
96502.984 |
17.469 |
12.422 |
2254714.244 |
|
|
|
|
|
|
|
|
|
| LP16 |
662138.232 |
247763.574 |
23217.103 |
653430.420 |
481555.835 |
52632.672 |
16.299 |
0.242 |
2121172.397 |
|
|
|
|
|
|
|
|
|
| LA1 |
542867.038 |
291631.774 |
16101.327 |
208393.119 |
534546.718 |
287585.245 |
20.487 |
11.217 |
1881559.346 |
|
|
|
|
|
|
|
|
|
| 水为 |
768502.457 |
174767.313 |
34293.763 |
538598.282 |
767009.154 |
130620.652 |
24.093 |
8.098 |
2414246.025 |
|
|
|
|
|
|
|
|
|
| LA3 |
836100.485 |
232547.859 |
8383.317 |
197659.044 |
475008.913 |
70420.274 |
26.668 |
18.771 |
1820654.452 |
|
|
|
|
|
|
|
|
|
| LA4 |
554969.006 |
231606.445 |
11451.728 |
365538.304 |
505163.079 |
269818.459 |
11.932 |
3.908 |
1938873.753 |
|
|
|
|
|
|
|
|
|
| LA5 |
794677.014 |
293809.893 |
30832.050 |
293530.490 |
510493.154 |
262663.280 |
12.498 |
6.307 |
2186491.358 |
|
|
|
|
|
|
|
|
|
| LA6 |
704360.729 |
287183.610 |
31019.483 |
189142.179 |
384371.287 |
327789.933 |
12.239 |
4.852 |
1924324.212 |
|
|
|
|
|
|
|
|
|
| LA7 |
894339.623 |
204701.453 |
27985.214 |
281212.437 |
595254.900 |
371594.312 |
24.934 |
18.350 |
2375510.268 |
|
|
|
|
|
|
|
|
|
| LA8 |
427283.433 |
184599.086 |
23700.009 |
211637.098 |
596714.072 |
325251.967 |
22.097 |
1.616 |
1769588.097 |
|
|
|
|
|
|
|
|
|
| LA9 |
879491.821 |
180076.383 |
12682.543 |
611024.725 |
416252.729 |
426535.290 |
22.887 |
10.851 |
2526553.669 |
|
|
|
|
|
|
|
|
|
| LA10 |
712373.467 |
297199.560 |
9966.649 |
218385.532 |
678770.388 |
129164.596 |
32.940 |
4.216 |
2046274.948 |
|
|
|
|
|
|
|
|
|
| LA11 |
625486.302 |
275066.946 |
25810.603 |
496586.040 |
470812.328 |
438093.869 |
14.927 |
1.233 |
2332275.197 |
|
|
|
|
|
|
|
|
|
| LA12 |
796469.226 |
199565.531 |
26532.869 |
616737.846 |
538184.180 |
286481.065 |
25.413 |
16.087 |
2464438.679 |
|
|
|
|
|
|
|
|
|
| LA13 |
587462.535 |
234893.095 |
16533.152 |
573600.610 |
441381.833 |
215218.753 |
5.375 |
3.154 |
2069549.281 |
|
|
|
|
|
|
|
|
|
| LA14 |
598797.362 |
200101.308 |
32285.037 |
179408.885 |
737688.278 |
321911.868 |
10.937 |
7.808 |
2070719.888 |
|
|
|
|
|
|
|
|
|
| LA15 |
775176.535 |
182419.544 |
9875.652 |
375172.731 |
686178.073 |
146549.529 |
20.767 |
18.862 |
2175875.636 |
|
|
|
|
|
|
|
|
|
| LA16 |
486462.514 |
269482.486 |
19514.461 |
382028.835 |
385846.710 |
419515.606 |
25.566 |
4.690 |
1963357.619 |
|
|
|
|
|
|
|
|
|
| LA17 |
397975.248 |
220647.104 |
20225.722 |
404790.872 |
406870.509 |
69049.196 |
25.782 |
16.448 |
1520009.288 |
|
|
|
|
|
|
|
|
|
| LA18 |
852308.317 |
145491.528 |
11612.476 |
499143.838 |
570244.949 |
84828.689 |
23.929 |
16.569 |
2164078.568 |
|
|
|
|
|
|
|
|
|
|
|
|
,分析物没有检测到。
|
样本中的元素含量较高的钠、钙、钾、铁、镁和铝。他们的浓度范围是366808.463 - -897950.747,373586.252 - -799614.153,169080.807 - -737471.818,35506.241 - -439955.497,145491.528 - -308322.609,8383.317 - -34467.794μ分别g / L。浓度范围的锶、钡、锌、锰和钛是154.053 - -279.990,32.520 - -142.280,22.616 - -95.305,2.110 - -64.537,16.833 - -45.839μg / L。的浓度钒、铬、钴、镍、铜、砷、硒、钼、镉、锡、锑、铊、铅很低。它从0.0到3.492不等μg / L。显然,在样本高宏单元的内容,它们含有微量元素要低得多。
3.6。统计分析
作为一个最重要的多变量分析技术,采用主成分分析评价三种浮萍之间的变化。主成分分析是一种无监督的模式识别方法事先信息多维数据的数据集和留存最大方差,同时减少其维度分为两或三维29日- - - - - -31日]。此外,所有的数据信息氨基酸受到监督方法,OPLS-DA [32),以获得更多的信息关于不同浮萍。所有的原始数据都由归一化处理和帕累托缩放(Par)建模。三个维度建立了PCA和OPLS-DA模型基于自由氨基酸的含量和比例的自由与R2X总氨基酸(和)值为0.628,0.596和0.758,0.676,分别。如数据所示2- - - - - -5PCA和OPLS-DA方法证明Spirodela polyrhiza,Landoltia punctata,浮萍属aequinoctialis聚集在一起互相的阴谋。在不同的浮萍无显著差异。
4所示。结论
在这项研究中,一个简单、快速、方便的方法与UPLC-QTRAP-MS /女士建立了浮萍的识别和量化控制HILIC分离不衍生化。方法验证的结果表明,开发的方法是敏感、准确、精确测定24自由氨基酸。59批次的样品在三个物种被量化,然后利用PCA和OPLS-DA数据统计分析。多变量分析表明自由氨基酸的含量之间没有明显不同Spirodela polyrhiza,Landoltia punctata,浮萍属aequinoctialis在模式识别中。但作为一个食物,更多的氨基酸意味着更多的营养和更有价值。换句话说,所有的三种浮萍可以作为食品同样如果AAs的绝对数量或多或少是相同的。总之,一个可靠和可行的方法开发了浮萍的营养评价。
数据可用性
使用的数据来支持本研究的结果包括在本文中。
的利益冲突
作者宣称没有利益冲突有关的出版。
确认
这项研究得到了中国国家自然科学基金(81073002),该计划的协同创新中心的中国江苏省药用物质资源产业化(2016),和全国“二十5年”技术支持项目(2011 bai04b06)。
PDF
,1,2,3
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