collagen-based矩阵的组件对培养小鼠结肠瀑样。(a)的比较人工基底膜(上半部分),collagen-based矩阵没有火腿的F12(中间面板),和collagen-based矩阵与火腿的F12(面板)显示不同的表型。所有矩阵都是混合比率的瀑样1:1。(b)不同百分比的胶原蛋白(30%,70%,和80%)区分不同生长在每一个百分比。在通道0随访瀑样每天在显微镜下。(c)测量环境的瀑样显示百分比的影响火腿的F12(0%、5%和10%)。(d)测量环境的瀑样与每个胶原百分率(30%、70%和80%)。(e)碳酸氢盐浓度测试和跟进每日在显微镜下。(f)的测量情况下瀑样在不同浓度的碳酸氢钠是相同的。(g)相对mRNA表达的标记代表瀑样测定人工基底膜的0%,5%和10%碳酸氢钠。 Lgr5 was used as a marker of intestinal stem cells, E-cadherin (Ecad) as a marker of epithelial cells, villin-1 (Vil1) as a marker of the epithelium border, defensin-5 (Defensin5) as a marker of Paneth cells, and chromogranin A (ChrgA) as a marker of enteroendocrine cells. The circumstance of the organoids was measured on the last day in vitro. Day denotes days of in vitro. Bar scale is 50μm。 , , 。