|
| 基因名字 |
引物序列 |
退火T (°C) |
基因库加入数量 |
|
| Oct4 |
F: 5
- - - - - -CATTGAGAACCGTGTGAG-3R: 5
- - - - - -TGAGTGATCTGCTGTAGG-3 |
60°C |
NM_013633.3 |
| Sox2 |
F: 5
- - - - - -AGGGCTGGGAGAAAGAAGAG-3R: 5
- - - - - -CCGCGATTGTTGTGATTAGT-3 |
60°C |
NM_011443.4 |
| Sall4 |
F: 5
- - - - - -CTAAGGAGGAAGAGGAGAG-3R: 5′-CAAGGCTATGGTCACAAG-3 |
60°C |
NM_175303.4 |
| Nanog |
F: 5
- - - - - -CTCAAGTCCTGAGGCTGACA-3R: 5
- - - - - -TGAAACCTGTCCTTGAGTGC-3 |
60°C |
NM_028016.3 |
| Rex1 |
F: 5
- - - - - -CAGCCAGACCACCATCTGTC-3R: 5
- - - - - -GTCTCCGATTTGCATATCTCCTG-3 |
60°C |
NM_009556.3 |
| Dppa5a |
F: 5
- - - - - -CCGTGCGTGGTGGATAAG-3R: 5
- - - - - -GCGACTGGACCTGGAATAC-3 |
60°C |
NM_025274.3 |
|
