击倒的DNMTs诱发衰老在msc。的差别(a)对这些DNMTs衰老msc。诱导细胞衰老后,细胞收获和DNMTs是由半定量PCR的表达。(b)击倒DNMTs的成分。慢病毒包含DNMT shrna (sh-DNMT1 sh-SNMT3a, sh-DNMT3b)或争夺控制(sh-CT)传导到HMSCs。72 h后,转导效率评估的观察GFP阳性细胞。细胞收获和RNA提取,DNMTs决心通过rt - pcr的表达。(c)诱导细胞衰老DNMT-shRNA击倒。左面板:HMSCs形态学DNMT-shRNA转导后7天。Sh-CT: shRNA争夺控制; sh-DNMTs: HMSCs were transducted by lentiviruses containing DNMT1, DNMT3a, and DNMT3b shRNAs. Middle panel: lentiviral transduction efficiency as shown by copGFP fluorescence of the shRNA vector. Right panel: senescent-associatedβ加染色。DNMT-shRNA击倒后,msc被染色β加活动。注意衰老的发生在过氧化DNMT-knockdown msc在缺乏治疗。考克斯(d)的表达和阐述DNMT-shRNA-treated msc。β肌动蛋白是用作PCR反应的内部控制。Cox1在DNMT-knockdown msc调节与细胞衰老。