在衰老msc mtDNA甲基化改变。(一)原理图的线粒体基因和CpG岛的位置。为了检测mtDNA甲基化在衰老细胞,我们设计了11条methylation-specific引物位于不同的基因在线粒体。(b)的比较mtDNA控制和衰老HMSCs之间的甲基化。亚硫酸氢mtDNA甲基化是衡量联合限制分析(眼镜蛇)。从mtDNA PCR产品的控制和衰老HMSCs被TaqI消化或HpyCH4IV (HPY)分离unmethylated和甲基化dna。Taq我HpyCH4IV识别和消化甲基化ACGT TCGA网站,分别。与硫酸氢钠治疗后,unmethylated胞核嘧啶转化为尿嘧啶,和TTGA ATGT网站不是由这两个酶消化。消化后,unmethylated和甲基化DNA 3%琼脂糖凝胶上分离。只有数据CpG岛4 2和1了。 (c) Differential mtDNA methylation between the control and senescent SMSCs. (d) Altered mtDNA methylation in human neonatal and adult fibroblasts. (e) Quantitation of mtDNA CpG methylation. The methylated and unmethylated bands were scanned. The status of CpG methylation was calculated as the relative percentage of DNA methylation using the untreated MSCs as 100.与未经处理的MSC控制细胞。注意减少mtDNA甲基化在衰老msc。