DE皮下移植。()方法随后插入皮下注射。(b)的代表形象hUCMS保持在正常培养条件和染色Ku80人类nuclear-specific蛋白质。这种蛋白质的典型核形状染色是显而易见的。(c) hUCMS Ku80支架保持一个明确的积极的环境干扰。细胞和支架的出现沾Ku80显示完全欣赏德执行同样的分析。(d)检索在表示时间。德上涂覆皮肤治疗的所有动物和检查时间。牺牲的时候,植皮面积被推翻,这允许升值DE附着到皮肤覆盖。在植入区,不可能发现排斥反应或炎症的迹象。相反,一个重要的血管化在7天,消失的贪污伤口愈合的进展,出现了。 The DE is clearly visible at all times of the analysis. Representative images of the central sections of DE stained with H&E, Masson, and keratins. In any histological image of H&E, numbered rectangles indicate the respective magnification on the side. For some representative areas, immunofluorescence analysis for Ku80 showing the persistence of hUCMS up to 21 days is shown. In explants made at 42 days, positive Ku80 is very doubtful. In the sections of the central area of DE made with Masson staining or keratin staining, the squares indicate the areas that are presented enlarged in Figure3。树老鼠用于每个时间点。