补充血清对人包皮成纤维细胞的生长动力学。人包皮成纤维细胞保持酶的分离和镀到培养皿中。高频电炉在媒体培养包含人类脊髓血液血清(hUCS;HFF-hUCS)、胎牛血清(的边后卫;HFF-FBS),分离血清替代(KSR)或无血清的补充。分离细胞观察依恋行为和生长动力学。之间的差异在细胞附件HFF-hUCS和HFF-FBS被观察到。在电镀后5小时,几乎所有的分离HFF-FBS被附加到培养皿表面和展出fibroblastic-like形态,而大多数HFF-hUCS仍然悬浮在培养基。在镀后24小时内,所有HFF-FBS和HFF-hUCS附着在培养皿中并显示典型的成纤维细胞细胞形态学(a)。HFF-hUCS和HFF-FBS之间观察到类似的扩散模式。HFF-hUCS HFF-FBS逐步扩散,达到confluency播种后3到4天。 The proliferation behavior at early passage (p4 + 1) was similar to that at intermediate passage (p4 + 10) (b). In contrast to HFF-hUCS and HFF-FBS, HFFs cultured in the medium containing 10% KSR and without serum supplementation could not reach the confluency even being cultured for 7 days (c). HFF = human foreskin fibroblasts, FBS = fetal bovine serum, hUCS = human cord blood-derived serum, KSR = knockout serum replacement, and p = passage number. Scale bars = 200 μm。