研究文章
验证的反相高效液相色谱法同时分析半胱氨酸和减少谷胱甘肽在小鼠器官
表2
总结的精度、准确性和复苏的测定谷胱甘肽和半胱氨酸鼠标脾脏、淋巴结,胰腺和大脑(
)。
|
| 样本 |
浓度(μ米) |
盘中(RSD %) |
Interday (RSD %) |
准确性(RE %) |
恢复(%,平均±标准差) |
|
| 半胱氨酸 |
|
|
|
|
|
| 脾 |
2.5 |
4.6 |
11.7 |
3.8 |
98.4±1.3 |
| 20. |
5.3 |
8.0 |
5.8 |
96.8±2.4 |
| 40 |
4.6 |
3.9 |
2.6 |
97.7±2.1 |
| 淋巴结 |
2.5 |
6.9 |
8.9 |
5.1 |
97.0±2.3 |
| 20. |
9.0 |
12.9 |
4.8 |
96.8±2.6 |
| 40 |
5.0 |
11.7 |
6.2 |
93.9±5.7 |
| 胰腺 |
2.5 |
9.1 |
10.7 |
4.3 |
94.2±5.6 |
| 20. |
7.5 |
6.8 |
8.5 |
94.5±4.2 |
| 40 |
6.2 |
9.9 |
5.7 |
95.6±2.7 |
| 大脑 |
2.5 |
9.7 |
6.7 |
4.6 |
95.9±2.6 |
| 20. |
10.3 |
10.6 |
3.4 |
94.5±4.0 |
| 40 |
8.7 |
11.8 |
6.7 |
96.4±2.8 |
| 谷胱甘肽 |
|
|
|
|
|
| 脾 |
5 |
3.3 |
7.5 |
3.5 |
92.7±2.2 |
| 20. |
10.8 |
9.7 |
3.3 |
93.5±4.6 |
| 60 |
9.0 |
12.4 |
1.9 |
94.7±5.2 |
| 淋巴结 |
5 |
10.8 |
12.4 |
8.9 |
90.4±2.0 |
| 20. |
1.9 |
6.2 |
3.7 |
96.4±1.5 |
| 60 |
1.5 |
9.7 |
4.5 |
93.7±4.8 |
| 胰腺 |
5 |
9.9 |
12.8 |
3.3 |
91.4±2.8 |
| 20. |
7.5 |
9.4 |
5.0 |
97.4±3.2 |
| 60 |
9.3 |
13.7 |
6.4 |
91.1±6.3 |
| 大脑 |
5 |
2.6 |
12.6 |
13.7 |
86.5±5.0 |
| 20. |
4.4 |
10.7 |
1.2 |
98.2±3.7 |
| 60 |
9.0 |
8.1 |
5.3 |
94.3±7.2 |
|
|
