研究文章
端粒的G-Quadruplexes:从人类四膜虫重复
表2
peg - 200对DNA的熔化温度低聚物的钾和钠离子。
|
| 低聚物 |
(°C)在50 mM氯化钾+挂钩- 200 |
(°C)在50 mM氯化钠+挂钩- 200 |
| 0% |
15% |
30% |
50% |
0% |
15% |
30% |
50% |
| 295/265 |
295/265 |
295/265 |
295/265 |
295/265 |
295/265 |
295/265 |
295/265 |
|
| HTR |
63.2/62.9 |
63.2 / ND |
78.8 / ND |
ND / 89.8 |
50.4 / ND |
51.2 / ND |
52.8 / ND |
64.0 / ND |
| HTR1 |
71.8/73.1 |
71.4/76.3 |
64.3/83.1 |
ND / > 95 |
55.7 / ND |
58.9 / ND |
61.5 / ND |
64.4 / ND |
| HTR2 |
65.2/65.9 |
64.2/72.1 |
ND / 79.6 |
ND / > 95 |
51.2 / ND |
54.3 / ND |
58.1 / ND |
62.7 / ND |
| HTR3 |
67.8/68.6 |
69.3/75.3 |
ND / 81.7 |
ND / > 95 |
50.6 / ND |
54.3 / ND |
58.7 / ND |
64.8/71.6 |
| HTR1、2 |
69.5/70.5 |
ND / 76.9 |
ND / 83.2 |
ND / > 95 |
55.9 / ND |
59.1 / ND |
62.8 / ND |
65.3/72.1 |
| HTR1、3 |
72.2/71.5 |
70.5/79.1 |
ND / 86.4 |
ND / > 95 |
53.7 / ND |
56.2/59.8 |
60.1/63.2 |
ND / 73.5 |
| HTR2、3 |
81.3/76.9 |
ND / 79.7 |
ND / 85.2 |
ND / > 95 |
53.3 / ND |
57.3 / ND |
59.9/63.0 |
64.4/71.0 |
| HTR0、1、3 |
73.0/80.0 |
ND / 79.0 |
ND / 87.9 |
ND / > 95 |
52.5 / ND |
56.3/60.7 |
60.1/63.3 |
ND / 72.7 |
| HTR0、2、3 |
70.7/72.7 |
ND / 77.6 |
ND / > 95 |
ND / > 95 |
51.8/54.5 |
56.0/51.1 |
59.4/60.2 |
ND / 73.8 |
| HTR1、2、3 |
73.9/77.8 |
ND / 83.9 |
ND / 88.6 |
ND / > 100 |
56.4 / ND |
59.0 / ND |
61.2/64.0 |
ND / 75.7 |
| 用力推 |
83.1 / ND |
87.7 / ND |
> 95 |
ND |
57.4 / ND |
59.7 / ND |
ND / 66.8 |
ND |
|
|
