MGP vitronectin交联由DSP,纤连蛋白、纤连蛋白III1-C多肽。(a) sds - page DSP的交联反应的产品标记蛋白质和微量的放射性MGP孵化。交联剂的存在DSP,还原剂2-mercaptoethanol(2),表示+或−在适当的车道上。蛋白质存在于反应是上述车道;−,没有补充蛋白质;VN vitronectin;IIIC纤连蛋白III1-C多肽;FN,纤连蛋白;和OA,卵白蛋白。(b)凝胶过滤柱III1-C DSP交联MGP、纤粘连蛋白的分离。 Lines indicate 7 marker positions of 200, 116, 97, 66, 43, 31, and 14 kDa. DSP reacted sample containing labeled MGP and unlabeled III1-C (●); DSP reacted solution containing labeled MGP and unlabeled III1-C reduced with mercaptoethanol prior to column loading, (◯); labeled MGP alone (▵); Labeled MGP and unlabeled III1-C incubated with DSP vehicle (DMSO) (◊); DSP reacted solution containing labeled MGP alone (□). The exclusion volume被蓝色葡聚糖洗脱,估计和总量估计了酚红洗脱。列运行4 M胍包含缓冲区电离共价相互作用(见方法)。