在稳定性和嗜热P450 CYP119的功能N末端和C末端多组氨酸标签的影响

Amplex Red试氧化由H期间，在紫外 - 可见光谱的变化₂Ø₂在WT CYP119（A）和N-HIS-CYP119（B）的存在;the UV-visible spectra taken at 0, 2, 5, 10, 20, 30, 45, 60, and 90 minutes after addition of H₂Ø₂。反应组分分别为10 μM Amplex Red, 1.5 mM H₂Ø₂和1.2 μM enzyme in 50 mM sodium phosphate buffer at pH 7.4. Time course of changes in absorbance at 570 nm (c) and 415 nm (d) in the same reaction (△, N-His-CYP119; ◯, WT CYP119).