MMC-exposed鼠肺显示区域的微血管内皮细胞增殖。Immunofluorescent染色的冷冻大鼠肺部分和共焦成像。红色:CD34(内皮细胞)。怀特:α光滑的肌肉肌动蛋白(平滑肌细胞)。格林:点击它EdU染色(细胞核增殖细胞)。复染色= 4′,6-diamidino-2-phenylindole (DAPI)。(一)控制老鼠的肺。((b) - (f)) MMC-exposed鼠肺。(一)控制鼠肺实质显示很少的微血管内皮细胞增殖。注意单个内皮细胞在增殖(箭头所指)。((b) - (c))肺毛细管hemangiomatosis像焦点从MMC-exposed老鼠显示强烈的微血管内皮细胞增殖。(d)外膜成纤维细胞增殖在小改制的肺动脉。((e) - (f))外膜成纤维细胞增殖在小装修好的肺静脉。 (g) Quantification of the percentage of lung proliferating cells. ((h)-(i)) Exuberant adventitial fibroblast cell proliferation in MMC-exposed rats (i) compared to control rats (h) (Vimentin+EdU+ cells). Adventitial fibroblast cells were stained with antibody against Vimentin (fibroblast marker in red) and proliferative cells with click-it EdU (white nuclei = EdU positive nuclei = proliferating cells). Vessel media and adventitia (Adv) were delimited by the yellow dotted line area.。红酒吧= 100μ= 50 m。白色规模酒吧μm。