联合牛磺酸和Monoisoamyl Dmsa保护砷诱导氧化损伤的老鼠

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砷是一种天然元素在环境中无所不在地礼物。高浓度的天然饮用水中砷是一个主要的健康问题在世界不同的地方。尽管砷的健康危害和有据可查的致癌物,不安全的,有效的和具体的预防或治疗措施是可用的。最近在各种策略的采用,抗氧化剂已经报告给管理是最有效的。本研究旨在评价治疗效果的monoisoamyl dimercaptosuccinic酸(MiADMSA),管理单独或结合牛磺酸后在大鼠慢性砷暴露。砷暴露的雄性老鼠(25 ppm,亚砷酸钠在饮用水24周)与牛磺酸治疗(100毫克/公斤,i.p。,once daily), monoisoamyl dimercaptosuccinic acid (MiADMSA) (50 mg/kg, oral, once daily) either individually or in combination for 5 consecutive days. Biochemical variables indicative of oxidative stress along-with arsenic concentration in blood, liver and kidney were measured. Arsenic exposure significantly reduced blood δ-aminolevulinic acid dehydratase (ALAD) activity, a key enzyme involved in the heme biosynthesis and enhanced zinc protoporphyrin (ZPP) level. Clinical hematological variables like white blood cells (WBC), mean cell hemoglobin (MCH), and mean cell hemoglobin concentration (MCHC) showed significant decrease with a significant elevation in platelet (PLT) count. These changes were accompanied by significant decrease in superoxide dismutase (SOD) activity and increased catalase activity. Arsenic exposure caused a significant decrease in hepatic and renal glutathione (GSH) level and an increase in oxidized glutathione (GSSG). These biochemical changes were correlated with an increased uptake of arsenic in blood, liver and kidney. Administration of taurine significantly reduced hepatic oxidative stress however co-administration of a higher dose of taurine (100 mg/kg) and MiADMSA provided more pronounced effects in improving the antioxidant status of liver and kidney and reducing body arsenic burden compared to the individual treatment of MiADMSA or taurine. The results suggest that in order to achieve better effects of chelation therapy, co-administration of taurine with MiADMSA might be preferred.

氧化医学和细胞寿命

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