文摘
慢性肾功能衰竭(CRF)代表一个世界卫生问题。臭氧增加内源性抗氧化防御系统,保护细胞的氧化还原状态。本研究的目的是评估臭氧/氧气混合的影响肾功能,形态、生化参数,CRF的实验模型(小计切除)。臭氧/氧混合物应用日报,由直肠吹气(
mg/kg) for 15 sessions after the nephrectomy. Renal function was evaluated, as well as different biochemical parameters, at the beginning and at the end of the study (10 weeks). Renal plasmatic flow (RPF), glomerular filtration rate (GFR), the urine excretion index, and the sodium and potassium excretions (as a measurement of tubular function) in the ozone group were similar to those in Sham group. Nevertheless, nephrectomized rats without ozone (positive control group) showed the lowest RPF, GFR, and urine excretion figures, as well as tubular function. Animals treated with ozone showed systolic arterial pressure (SAP) figures lower than those in the positive control group, but higher values compared to Sham group. Serum creatinine values and protein excretion in 24 hours in the ozone group were decreased compared with nephrectomized rats, but were still higher than normal values. Histological study demonstrated that animals treated with ozone showed less number of lesions in comparison with nephrectomized rats. Thiobarbituric acid reactive substances were significantly increased in nephrectomized and ozone-treated nephrectomized rats in comparison with Sham group. In the positive control group, superoxide dismutase (SOD) and catalase (CAT) showed the lowest figures in comparison with the other groups. However, ozone/oxygen mixture induced a significant stimulation in the enzymatic activity of CAT, SOD, and glutathione peroxidase, as well as reduced glutathione in relation with Sham and positive control groups. In this animal model of CRF, ozone rectal administrations produced a delay in the advance of the disease, protecting the kidneys against vascular, hemorheological, and oxidative mechanisms. This behavior suggests ozone therapy has a protective effect on renal tissue by downregulation of the oxidative stress shown in CRF.">
慢性肾功能衰竭(CRF)代表一个世界卫生问题。臭氧增加内源性抗氧化防御系统,保护细胞的氧化还原状态。本研究的目的是评估臭氧/氧气混合的影响肾功能,形态、生化参数,CRF的实验模型(小计切除)。臭氧/氧混合物应用日报,由直肠吹气(