γ have been reported elevated in psoriatic patients.Aim: To evaluate serum cytokine profiles in psoriasis patients by improved enzyme-linked immunosorbent assay (ELISA) technique and to correlate these levels with disease severity.Methods: We analyzed single serum samples from 10 patients with active untreated psoriasis, two patients with active treated psoriasis, and five healthy volunteers for major T helper type 1 and T helper type 2 cytokines using the LINCOplex ELISA multi-analyte detection system that permits simultaneous detection of multiple cytokines from a single sample. The disease severity, including erythema, induration, scale, and surface area, was assessed.Results: IFN-γ was markedly elevated in all sera from psoriasis patients, 33.8±1.3 pg/ml (mean±standard error) versus 8±1.5 pg/ml for normal controls (P<0.01), and positively correlated with all indices of disease severity (Spearman r>0.6). IL-8 was also increased in psoriasis patients (24.4±1.8 pg/ml) versus normal controls (3.6±1.2 pg/ml) (p<0.05) and positively correlated with the degree of erythema (Spearman r>0.6). Mean IL-12 levels were decreased in sera from psoriasis patients (8.5±1.2 pg/ml) compared with normal controls (42.2±5.3 pg/ml) (P<0.01). Also, serum IL-10 levels were below detection levels in psoriatics compared with controls (6.4±1.3 pg/ml).Conclusions: This new ELISA system allowed rapid and reliable detection of numerous cytokines in single serum samples from patients with psoriasis. We observed that IFN-γ and IL-8 cytokines were elevated in psoriatics and correlated with parameters of disease severity while IL-10 and IL-12 were decreased."> 同时测量多个Th1、Th2血清细胞因子在银屑病和与疾病严重程度相关 - raybet雷竞app,雷竞技官网下载,雷电竞下载苹果
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体积 12 |文章的ID 735813年 | https://doi.org/10.1080/09629350310001619753

莎朗·e·雅各布Mehdi Nassiri弗朗西斯科a . Kerdel弗拉基米尔Vincek, ”同时测量多个Th1、Th2血清细胞因子在银屑病和与疾病严重程度相关”,炎症介质, 卷。12, 文章的ID735813年, 5 页面, 2003年。 https://doi.org/10.1080/09629350310001619753

同时测量多个Th1、Th2血清细胞因子在银屑病和与疾病严重程度相关

莎朗·e·雅各布,1 Mehdi Nassiri,2 旧金山a Kerdel,1 和 弗拉基米尔•Vincek2

1皮肤和皮肤的手术,迈阿密大学/杰克逊纪念医疗中心,霍尔兹中心,美国西北12大街1611号2042室

2迈阿密大学病理学系/杰克逊纪念医疗中心,霍尔兹中心,美国西北12大街1611号2042室

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背景:银屑病斑块已被证明含有促炎细胞因子水平的增加。血清白介素(IL) 6日IL-7,引发和干扰素(IFN) γ 已报告在银屑病患者升高。目的:评估银屑病患者血清细胞因子资料,提高酶联免疫吸附试验(ELISA)技术和关联这些水平与疾病严重程度。方法:我们分析了单一治疗牛皮癣患者血清样本从10活跃,两个积极的治疗牛皮癣患者,和五个健康志愿者为主要辅助T 1型和2型细胞因子T辅助使用LINCO丛ELISA multi-analyte探测系统,允许同时检测多种细胞因子从单个样本。疾病严重程度,包括红斑、硬结、规模,和表面积,评估。结果:干扰素- γ 在从银屑病患者血清明显升高,33.8±1.3 pg / ml(平均值±标准错误)与正常对照组8±1.5 pg / ml吗 ( P < 0.01 ) ,积极与疾病严重程度的指标(枪兵 r > 0.6 )。引发牛皮癣患者也增加(24.4±1.8 pg / ml)和正常对照组(3.6±1.2 pg / ml) ( p < 0.05 ) 和正相关程度的红斑(枪兵 r > 0.6 )。意味着在银屑病患者血清il - 12水平下降(8.5±1.2 pg / ml)与正常对照组(42.2±5.3 pg / ml) ( P < 0.01 ) 。此外,血清il - 10水平低于检测水平与控制相比,银屑病(6.4±1.3 pg / ml)。结论:这个新的ELISA系统允许快速和可靠的众多细胞因子检测单从银屑病患者血清样本。我们发现干扰素- γ 和引发细胞因子升高在银屑病和疾病严重程度的相关参数,降低il - 10、il - 12。

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