评价人类肺泡巨噬细胞炎性细胞因子分泌的

文摘

肺泡巨噬细胞(AM)分泌白介素- 1β(IL-1β),肿瘤坏死factor-α(TNF-α)、白细胞介素- 6 (il - 6)和interleukin-8(引发),所有这些炎性细胞因子参与了许多肺部疾病的发病机理。当前工作的目的是评估的基础和刺激分泌细胞因子由人类AMs。收集的人类AMs支气管肺泡灌洗(BAL)从四个健康对照组和13弥漫性间质性肺疾病患者(5例结节病,三个过敏性肺炎和五个特发性肺纤维化)。AMs的存在与否是培养不同浓度的脂多糖(LPS), phorbolmyristate gammainterferon。IL-1βTNF-α,il - 6和引发水平测量BAL流体和文化上层清液使用特定的酶联免疫吸附试验。发现的物质刺激炎性细胞因子的分泌在最大的程度上有限合伙人在10μg /毫升的浓度。关于IL-1β分泌的,感兴趣的四个观测:基底分泌很低;有限合伙人产生了强有力的刺激效果;相当大的类内变化被观察到;还有比较组间无显著差异。 With respect to TNF-α secretion, the results were similar. The only striking finding was the higher basal secretion of this cytokine with respect to that of IL-1β. Regarding the secretion of IL-6, the same pattern followed by TNF-α was found. However, it should be stressed that the increase induced by LPS was smaller than in the two previous cytokines. Regarding the secretion of IL-8, three findings were patent: the strong basal secretion of this cytokine; the moderate increase induced by LPS; and the existence of significant differences among the different groups with respect to the stimulated secretion of this cytokine, which reached maximum values in patients with idiopathic pulmonary fibrosis. Finally, it should be noted that the pattern of cytokines observed in the BAL fluid was similar to that found in cultured AM supernatants. The pattern of inflammatory cytokine secretion by AMs differs from that of other cells of the mononuclear phagocyte system (MPS). In this sense. AMs secrete low amounts of IL-1, moderate amounts of TNF-α and IL-6, and high quantities of IL-8. Adherence is an important stimulus in the secretion of these molecules and LPS elicits an increased secretion inverse to the basal secretion. There is considerable individual variability in the secretion of inflammatory cytokines by the AMs of patients with interstitial lung disease and the AMs of these patients are primed在活的有机体内这些细胞因子的分泌。我们的研究的结果,执行在体外,可以外推到在活的有机体内设置。

文摘

版权

更多相关文章