αβ heterodimer, specific for the male (H-Y) antigen in association with H-2D^b, was determined. The transgenic α chain was expressed on about 10% of the fetal thymocytes on day 14 of gestation. About 50% of day-15 fetal thymocytes expressed both α and β transchains and virtually all fetal thymocytes expressed the transgenicαβ heterodimer by day 17. The early expression of the transgenic TCR on CD4^-8^- thymocytes prevented the development of γδ cells, and led to accelerated growth of thymocytes and an earlier expression of CD4 and CD8 molecules. Up to day 17, no significant differences in T-cell development could be detected between female and male thymuses. By day 18 of gestation, the male transgenic thymus contained more CD4^-8^- thymocytes than the female transgenic thymus. The preponderance of CD4^-8^- thymocytes in the male transgenic thymus increased until birth and was a consequence of the deletion of the CD4⁺8⁺ thymocytes and their CD4^-8⁺ precursors. By the time of birth, the male transgenic thymus contained half the number of cells as the female transgenic thymus. The deletion of autospecific precursor cells in the male transgenic mouse began only at day 18 of gestation, despite the fact that the ligand could already be detected by day 16.The preferential accumulation of CD4^-8⁺ T cells, which expressed a high density of the transgenic TCR, occurred only after birth and was .obvious in 6-week-old female thymus. These data support the hypothesis that the positive selection of T cells expressing this transgenic heterodimer may involve two steps, i.e., the commitment of CD4⁺8⁺ thymocytes to the CD4^-8⁺ lineage following the interaction of the transgenic TCR with restricting major histocompatibility molecules, followed by a slow conversion of CD4⁺8⁺ thymocytes into CD4^-8⁺ T cells.In normal mice, the precursors of CD⁺4⁺8 and single positive thymocytes have the CD4^-8^- CD3^-J11d⁺ (or M1/69 ⁺) phenotype. Because of the early expression of the transgenic αβ heterodimer, this population was not detected in adult transgenic mice. All CD4^-8^- M1/ 69⁺ cells expressed the transgenic receptor associated with CD3 and could be readily grown in media containing T-cell lectins and interleukin 2."> 表达雄性特异性受体的t细胞转基因小鼠的早期缺失和晚期阳性选择 - raybet雷竞app,雷竞技官网下载,雷电竞下载苹果

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体积 1 |文章的ID 018208 | https://doi.org/10.1155/1990/18208

Hung Sia Teh, Hiroyuki Kishi, Bernadette Scott, Peter Borgulya, Harald Von Boehmer, Pawel Kisielow，＂表达雄性特异性受体的t细胞在t细胞受体转基因小鼠中的早期缺失和晚期阳性选择"，免疫学研究杂志，卷。1，文章的ID018208， 10 页面， 1990． https://doi.org/10.1155/1990/18208

表达雄性特异性受体的t细胞在t细胞受体转基因小鼠中的早期缺失和晚期阳性选择

挂新航格兰，¹ 欲之岸，² 伯纳黛特•斯科特，² 彼得Borgulya，² 哈拉尔德·冯·》，¹ 和 Pawel Kisielow^3.

¹加拿大英属哥伦比亚大学微生物学系

²瑞士巴塞尔免疫学研究所

^3.波兰科学院免疫学和实验治疗研究所，波兰

收到了 1989年10月15日

接受 1989年10月23日

摘要

表达转基因T细胞受体(TCR)的转基因小鼠中T细胞的个体发生 α β 异源二聚体，特异性为与H-2D相关的男性(H-Y)抗原^b决定。转基因 α 在妊娠第14天，约10%的胎儿胸腺细胞表达链。约50%的15天胎儿胸腺细胞表达这两种基因 α 和 β 转链和几乎所有的胎儿胸腺细胞都表达转基因 α β 第17天为异源二聚体。转基因TCR在CD4上的早期表达^-8^-胸腺细胞阻止了 γ δ 并导致胸腺细胞加速生长和CD4和CD8分子的早期表达。直到第17天，女性和男性胸腺的t细胞发育没有显著差异。在妊娠第18天，雄性转基因胸腺中含有更多的CD4^-8^-胸腺细胞比雌性转基因胸腺细胞多。CD4占优势^-8^-雄性转基因胸腺中的胸腺细胞直到出生都在增加，这是CD4缺失的结果⁺8⁺胸腺细胞和它们的CD4^-8⁺体细胞。到出生的时候，雄性转基因胸腺的细胞数量是雌性转基因胸腺的一半。雄性转基因小鼠的自体特异性前体细胞的缺失仅在妊娠第18天开始，尽管配体在妊娠第16天就可以检测到。CD4的优先积累^-8⁺表达高密度转基因TCR的T细胞仅在出生后出现，在6周龄雌性胸腺中表现明显。这些数据支持了一种假说，即表达这种转基因异质二聚体的T细胞的阳性选择可能涉及两个步骤，即CD4的承诺⁺8⁺胸腺细胞转化为CD4^-8⁺转基因TCR与限制性主要组织相容性分子相互作用后，CD4的缓慢转化⁺8⁺胸腺细胞中CD4^-8⁺T细胞。在正常小鼠中，乳糜泻的前体⁺4⁺8和单个阳性胸腺细胞有CD4^-8^-CD3^-J11d⁺(或M1/69⁺)表型。因为转基因的早期表达 α β 在成年转基因小鼠中未检测到异二聚体。所有CD4^-8^-M1 / 69⁺细胞表达CD3相关的转基因受体，易于在含有t细胞凝集素和白细胞介素2的培养基中生长。

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