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no-repeat 6px;background-position:center;margin-top:10px;min-width:55px;display:none;} @media (min-width:768px){.gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption .expand_btn{display:none;}} .gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-text{-webkit-flex:9;-ms-flex:9;flex:9;text-align:left;padding:21px 37px 10px 0px;font-size:16px;font-weight:700;} @media (min-width:768px){.gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-text{padding:19px 18px 10px 18px;}} @media (min-width:992px){.gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-text{padding:19px 21px 10px 21px;}} .gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption 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no-repeat 6px;background-position:center;padding:16px 0 16px 0;min-width:62px;max-width:62px;} @media (min-width:768px){.gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-partial-url{padding:16px 0 17px 0;min-width:52px;max-width:52px;}} @media (min-width:992px){.gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-partial-url{padding:15px 0 17px 0;min-width:60px;max-width:60px;}} .gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-partial-url:hover{-webkit-text-decoration:none;text-decoration:none;} .gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption-text{font-size:14px;padding:0px 18px 17px 22px;} @media (min-width:768px){.gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption-text{padding:0px 15px 15px 18px;}} @media (min-width:992px){.gvEcCq .floats-partial-section .floats-partial-footer .floats-partial-caption-text{padding:0px 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href="//www.newsama.com/journals/ijg/" aria-label="International Journal of Genomics">国际基因组学杂志</一个>/<gydF4y2Ba一个cl一个ss="sc-htpNat bUhGXt link sc-eitiEO jXeALb breadCrumb" href="//www.newsama.com/journals/ijg/contents/year/2011/" aria-label="2011">2011年</一个>/<ggydF4y2BaydF4y2Baspan class="sc-bhlBdH UVThf">文章</gydF4y2Baspan> </div> <div class="threeColumn__ThreeColumnWrapper-sc-1bf4078-0 dDyxFF threeColumnWrapper"> <div class="threeColumn__LeftWrapper-sc-1bf4078-1 fkVFlF leftWrapper"> <button type="button" class="sc-eKZiaR dzZrgS section__button " id="sectionButton"><span class="sc-htoDjs diutvq icon__dots dots__detector icon-colored _3n-7Ho7DlfjMlZbrRr9Q4a icon-themeable" title="" font-size="22"></span>文章部分</gydF4y2Babutton> <div class="sc-bEjcJn jLUVhb static_contents__section_links links__hidden "> <div id="leftSectionMenu" class="sc-cbkKFq prcY sc-bGbJRg dVCLgr left_section_menu"> <p>在这一页上</gydF4y2Bap> <span class="sc-likbZx bBmZJZ grey" property="background-color" color="#D8D8D8"><a class="sc-htpNat bUhGXt link sc-ePZHVD ewHMLz" href="#abstract" aria-label="">文摘</一个></span> <span class="sc-likbZx bBmZJZ grey" property="background-color" color="#D8D8D8"><a class="sc-htpNat bUhGXt link sc-ePZHVD ewHMLz" href="#introduction" aria-label="">介绍</一个></span> <span class="sc-likbZx bBmZJZ grey" property="background-color" color="#D8D8D8"><a class="sc-htpNat bUhGXt link sc-ePZHVD ewHMLz" href="#materials-and-methods" aria-label="">材料和方法</一个></span> <span class="sc-likbZx bBmZJZ grey" property="background-color" color="#D8D8D8"><a class="sc-htpNat bUhGXt link sc-ePZHVD ewHMLz" href="#results-and-discussion" aria-label="">结果与讨论</一个></span> <span class="sc-likbZx bBmZJZ grey" property="background-color" color="#D8D8D8"><a class="sc-htpNat bUhGXt link sc-ePZHVD ewHMLz" href="#discussion" aria-label="">讨论</一个></span> <span class="sc-likbZx bBmZJZ grey" property="background-color" color="#D8D8D8"><a class="sc-htpNat bUhGXt link sc-ePZHVD ewHMLz" href="#acknowledgments" 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体积<gydF4y2Ba!-- --> 2011年<gydF4y2Ba!-- --> <span class="sc-fZwumE cUsFHx">|</gydF4y2Baspan>文章的ID<gydF4y2Ba!-- --> 212146年<gydF4y2Ba!-- --> <span class="sc-fZwumE cUsFHx">|</gydF4y2Baspan> <a class="sc-htpNat bUhGXt link sc-fQejPQ duuwge" href="https://doi.org/10.1155/2011/212146" aria-label="Doi-link">https://doi.org/10.1155/2011/212146</一个></div> <div class="sc-LKuAh lcOiDF article_full_citation"> <div class="sc-hzNEM gsmOCv isHide"> <p>马Pinchao Xuhua夏<gydF4y2Ba!-- -->,<gydF4y2Ba!-- --> ”<gydF4y2Baspan class="adjust-article-svg-size">影响酵母基因拼接强度的因素</gydF4y2Baspan>”,<gydF4y2Ba!-- --><i>国际基因组学杂志</我>,<ggydF4y2BaydF4y2Ba!-- --> 卷。<gydF4y2Ba!-- -->2011年<gydF4y2Ba!-- -->,<gydF4y2Ba!-- --> 文章的ID<gydF4y2Ba!-- -->212146年<gydF4y2Ba!-- -->,<gydF4y2Ba!-- --> 13<gydF4y2Ba!-- -->   页面<gydF4y2Ba!-- -->,<gydF4y2Ba!-- --> 2011年<gydF4y2Ba!-- -->。<gydF4y2Ba!-- --> https://doi.org/10.1155/2011/212146</gydF4y2Bap> </div> <button class="sc-hSdWYo etauus sc-iBEsjs kPshFw" color="">显示引用</gydF4y2Babutton> </div> <h1 class="sc-iRbamj jZOxOO sc-iSDuPN kEVvRd article_title adjust-article-svg-size heading-title">影响酵母基因拼接强度的因素</h1></gydF4y2Badiv> <div class="sc-jrIrqw eHMFfo article_authors"> <hr class="sc-cEvuZC XkrFe hr__author"> <span class="">Pinchao马<gydF4y2Basup>1</gydF4y2Basup>和</gydF4y2Baspan> <span class=""><b>Xuhua夏</gydF4y2Bab><a class="sc-htpNat bUhGXt link" href="mailto:" aria-label="Mail Option"><span class="sc-htoDjs kNoiZu sc-hjRWVT irNWJI icon-colored _2zt-1j0LmaF8UjTQAM413G icon-themeable" title="" font-size="16"></span></a><sup>1<gydF4y2Ba!-- -->,<gydF4y2Ba!-- -->2</gydF4y2Basup></span> <div class="sc-fHxwqH dMuGGA isHide"> <p><sup>1</gydF4y2Basup><span>渥太华大学生物学系,30居里夫人,邮政信箱450,车站,渥太华,加拿大</gydF4y2Baspan></p> <p><sup>2</gydF4y2Basup><span>渥太华系统生物学研究所,渥太华大学,渥太华,加拿大</gydF4y2Baspan></p> </div> <button class="sc-hSdWYo etauus sc-iQtOjA kfDwZY" color="">显示更多</gydF4y2Babutton> </div> <div class="articleContent__EditorWrapper-sc-2yl9jy-3 evMkvU"> <div class="articleContent__TextWrapper-sc-2yl9jy-8 fYrLtk"> <b>学术编辑器:</gydF4y2Bab>约翰·帕金森</gydF4y2Badiv> <div class="articleContent__PublicationTimeLineWrapper-sc-2yl9jy-4 fjviok"> <div class="articleContent__PublcationCol-sc-2yl9jy-5 hgKieC notPublished"> <span class="articleContent__PublicationField-sc-2yl9jy-6 cIribm">收到了</gydF4y2Baspan> <span class="articleContent__PublicationValue-sc-2yl9jy-7 khHUcu">2011年7月30日</gydF4y2Baspan> </div> <div class="articleContent__PublcationCol-sc-2yl9jy-5 hgKieC notPublished"> <span class="articleContent__PublicationField-sc-2yl9jy-6 cIribm">接受</gydF4y2Baspan> <span class="articleContent__PublicationValue-sc-2yl9jy-7 khHUcu">06年9月2011年</gydF4y2Baspan> </div> <div class="articleContent__PublcationCol-sc-2yl9jy-5 hgKieC isPublished"> <span class="articleContent__PublicationField-sc-2yl9jy-6 cIribm">发表</gydF4y2Baspan> <span class="articleContent__PublicationValue-sc-2yl9jy-7 khHUcu">2011年11月20日</gydF4y2Baspan> </div> </div> </div> <article class="sc-jtRlXQ gvEcCq article_body "> <div> <div class="xml-content"> <h4 class="header" id="abstract">文摘</h4><gydF4y2Bap>准确和有效的拼接至关重要高转录intron-containing基因(协调小组)迅速复制单细胞真核生物如出芽酵母<我>酿酒酵母</我>。我们描述5′和3′拼接网站(ss)位置权重矩阵得分(PWMSs),这是共识序列的最高和最低的接头地点不同大多数使用的共识序列和pwm作为拼接的代理力量。<我>H一个C1</gydF4y2Ba我>,这是拼接nonspliceosomal机制,其最消极的pwm 5′ss和3′党卫军。多个基因剪接调控和下需要附加因素的剪接还小或消极的pwm值。接合强度高高度转录协调小组比低转录协调小组和更高的成绩单,绑定强烈剪接体比绑定弱。3′拼接网站功能突出poly-U束前3′AG)。我们的研究结果表明潜在的协调小组,利用pwm作为筛查工具要么是拼接nonspliceosome机制或在剪接调控酵母和其他真菌的物种。</gydF4y2Bap> <span class="end-abs"></span> <h4 id="introduction">1。介绍</h4><gydF4y2Bap>真核基因的内含子剪接体拼接出主要的(<一个href="#B1">1</一个>)通过多种RNA-RNA、rna蛋白质和蛋白质的相互作用涉及5′拼接网站(ss)、3′ss和分叉点序列(<一个href="#B2">2</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B10">10</一个>]。酵母,<我>酵母,</我>似乎只有U2-type内含子(<一个href="#B11">11</一个>,<gydF4y2Ba一个href="#B12">12</一个>的共识序列),5′党卫军被5′和3′ss -∣GUAUGU和掺钕钇铝石榴石∣3′,分别为(<一个href="#B11">11</一个>,<gydF4y2Ba一个href="#B13">13</一个>,<gydF4y2Ba一个href="#B14">14</一个>]。5′党卫军强烈约束通过碱基配对U1和U6核内小rna (<一个href="#B15">15</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B17">17</一个>),导致绝大多数5′党卫军在酵母GUAUGU的共识。在多细胞真核生物和裂殖酵母,3′党卫军被U2AF强烈约束<gydF4y2Basup>35</gydF4y2Basup>蛋白质(<一个href="#B18">18</一个>]。但是,没有<我>U2一个F35</gydF4y2Ba我>同系物在出芽酵母(<一个href="#B19">19</一个>,<gydF4y2Ba一个href="#B20">20.</一个>),尽管3′党卫军是部分受到PRP8p蛋白(<一个href="#B21">21</一个>]。</gydF4y2Bap> <p>一个基因的蛋白质需要大规模生产不仅需要转录率高,而且拥有特性允许它拼接有效和准确的。因此,剪接是一个主要组件的mRNA生产质量控制流程的真核生物(<一个href="#B22">22</一个>]。高表达基因进化应该有效的5′ss和3′党卫军,避免异常剪接不仅浪费而且还可以产生错误的蛋白质,扰乱正常的细胞过程。相比之下,粘接强度高的选择应该是相对较弱的低表达基因的党卫军可能漂移通过突变来拼接效率低。这有两个关于粘接强度的影响。首先,粘接强度高转录基因应该是高于平均低转录的基因。第二,粘接强度的方差应该大的转录基因(其接合强度可能很高,但也可能漂移值低通过突变)比高转录基因(其接合强度应该高)。摘要首先系统分析之间的关系连接强度和基因表达的水平。</gydF4y2Bap> <p>全面评估内含子剪接的力量和基因表达之间的关系需要准确描述的内含子和可靠的大规模测量基因表达。酵母(<我>酿酒酵母</我>)是第一个物种的准确表征在信使rna和蛋白质内含子和基因表达水平。目前已经开发出了两个强大的方法描述酵母基因内区。首先是使用高密度酵母花砖数组与退化的酵母突变体缺陷处理基因内区套索(<一个href="#B23">23</一个>]。套索在RNA的积累池是高密度花砖阵列不仅可以检测到的基因内区验证还发现新的基因内区。第二种方法涉及到设计微阵列探针具体exon-intron连接和exon-exon连接定量描述unspliced和拼接mRNA (<一个href="#B24">24</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B26">26</一个>]。此外,<我>酿酒酵母</我>是为数不多的与大规模物种全基因组特征的mRNA转录(<一个href="#B27">27</一个>,<gydF4y2Ba一个href="#B28">28</一个>)和蛋白质丰度(<一个href="#B29">29日</一个>)或蛋白质合成率(<一个href="#B30">30.</一个>]。而转录和蛋白质特征已经被其他物种,没有物种的基因内区都被定性为准确、彻底的酵母。在这项研究中,我们使用酵母数据来研究基因表达和连接强度之间的关系。</gydF4y2Bap> <p>除了高质量的分子数据的可用性,在使用有额外的优势<我>酿酒酵母</我>对于这样一个研究。首先,酵母细胞需要迅速复制和自然选择应该强烈反对酵母高度表达的基因拼接内含子与不佳。第二,酵母基因组中几乎没有内含子,他们中的大多数已经正确的注释(<一个href="#B23">23</一个>,<gydF4y2Ba一个href="#B24">24</一个>,<gydF4y2Ba一个href="#B31">31日</一个>,<gydF4y2Ba一个href="#B32">32</一个>]。第三,剪接机制酵母高等真核生物(相比是相对简单的<一个href="#B24">24</一个>),与关键蛋白质剪接体更好的比任何其他生物特征<一个href="#B33">33</一个>]。第四,除了少数基因(<一个href="#B31">31日</一个>,<gydF4y2Ba一个href="#B34">34</一个>),可变剪接中观察到多细胞真核生物是罕见的在酵母(<一个href="#B35">35</一个>]。剪接机制<我>酿酒酵母</我>似乎是简单的甚至在真菌的物种,例如,其基因组没有U2AF的同系物<gydF4y2Basup>35</gydF4y2Basup>spliceosomal蛋白存在于其他真菌物种如裂殖酵母(<我>粟酒裂殖酵母</我>)和多细胞真核生物基因组测序<一个href="#B19">19</一个>,<gydF4y2Ba一个href="#B20">20.</一个>]。<我>酿酒酵母</我>也缺乏serine-arginine蛋白质作为必不可少的剪接因子在后生动物<一个href="#B36">36</一个>]。</gydF4y2Bap> <p>很难直接测量连接强度,和以前的出版物使用的位置权重矩阵(PWM, [<一个href="#B37">37</一个>,<gydF4y2Ba一个href="#B38">38</一个>)的详细数值插图)来自纳粹党卫军和由此产生的脉宽调制(PWM)得分作为拼接强度(代理<一个href="#B39">39</一个>,<gydF4y2Ba一个href="#B40">40</一个>]。如果绝大多数内含子剪接体拼接的机制,如果有一个最优的党卫军强烈首选的剪接体(即机制。,我ntrons with ss in their optimal state are most efficiently spliced), and if there is strong selection pressure to maintain such an optimal state for most of the genes (i.e., if mutations leading to deviation from such an optimal state are deleterious), then we should expect that most ss should converge towards the optimal state, that the ss with the optimal state will have the highest PWMS, and that those deviating from the optimal state should have low PWMS. In short, PWMS may be used as a proxy for splicing strength by the spliceosome mechanism if the three conditions are satisfied. Hereafter, splicing strength refers specifically to splicing strength by the spliceosome mechanism.</p> <h4 id="materials-and-methods">2。材料和方法</h4><h5gydF4y2Baid="sec2.1">2.1。5′和3′接头地点</h5><gydF4y2Bap>所有16条染色体的基因序列<我>酿酒酵母</我>被检索<一个href="ftp://ftp.ncbi.nlm.nih.gov/genomes/Fungi/Saccharomyces_cerevisiae_uid128/" target="_blank">ftp://ftp.ncbi.nlm.nih.gov/genomes/Fungi/Saccharomyces_cerevisiae_uid128/</一个>(组装日期:14 - 7 - 2011)。有279注释插入子打破270个基因的编码区,261个基因每个包含一个基因内区和九个基因(SUS1、VMA9、HMRA1 DYN2, YOS1, RPL7A, AML1, TAD3,和RPL7B)每个包含两个内含子。一些来自paralogous内含子的基因是相同的。基因YBL111C、YHR218W YLL067C、YLL066C YML133C paralogous包含相同的基因内区,所以是基因YIL177C YRF1-3 YJL225C和基因,YRF1-6, YRF1-7。这就产生了两个问题。首先是缺乏独立的数据统计分析。第二个涉及量化信使rna和蛋白质的生产。以基因YIL177C和YJL225C为例。很难知道信使rna和蛋白质丰度是由两个基因只有一个或两者兼而有之。然而,paralogues很少在酵母协调小组,并从分析不排除这些基因改变达成的结论。</p> <p>有24个基因的内含子5′utr(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab1/" target="_blank">1</一个>)。我们最初认为他们可能较弱的学生比那些位于编码序列,因为未能拼接等内含子似乎小功能结果只要翻译机器可以找到合适的翻译起始位点。然而,没有检测到两者之间的区别。排除或包括这些24酵母协调小组并没有改变的结论。</gydF4y2Bap> <div class="floats-partial-section partial-other-wrapper" data-section="tab1"> <div class="floats-partial-content fig-content-tab1"> <table class="table-group" id="tab1"> <tbody> <tr> <td> <table class="table"> <tbody> <tr> <td class="thead-hr" colspan="6"> <hr></td> </tr> <tr class="thead"> <td align="left">系统。的名字<gydF4y2Basup>(1)</gydF4y2Basup></td> <td align="center">性病的名字<gydF4y2Basup>(2)</gydF4y2Basup></td> <td align="center">空空的</tgydF4y2Bad> <td align="center">位置<gydF4y2Basup>(3)</gydF4y2Basup></td> <td align="center">基因组的位置</tgydF4y2Bad> <td align="center">链<gydF4y2Basup>(4)</gydF4y2Basup></td> </tr> <tr> <td class="thead-hr" colspan="6"> <hr></td> </tr> <tr> <td align="left">YBL072C</tgydF4y2Bad> <td align="center">RPS8A</tgydF4y2Bad> <td align="center">2</tgydF4y2Bad> <td align="center"><span style="width: 49.599998px;"> <svg 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align="center">W</tgydF4y2Bad> </tr> <tr> <td align="left">YER131W</tgydF4y2Bad> <td align="center">RPS26B</tgydF4y2Bad> <td align="center">5</tgydF4y2Bad> <td align="center"><span style="width: 49.599998px;"> <svg height="11.0375" id="M9" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 49.599998 11.0375" width="49.599998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0375)"> <g transform="translate(72,-63.17)"> <text transform="matrix(1,0,0,-1,-71.95,63.4)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 6</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; 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x="16.666498" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 4</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">223771 . . 223441</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> </tr> <tr> <td align="left">YGL031C</tgydF4y2Bad> <td align="center">RPL24A</tgydF4y2Bad> <td align="center">7</tgydF4y2Bad> <td align="center"><span style="width: 49.599998px;"> <svg height="11.0375" id="M11" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 49.599998 11.0375" width="49.599998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0375)"> <g transform="translate(72,-63.17)"> <text transform="matrix(1,0,0,-1,-71.95,63.4)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 6</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 8</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">438397 . . 437942</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> </tr> <tr> <td align="left">YGL187C</tgydF4y2Bad> <td align="center">COX4</tgydF4y2Bad> <td align="center">7</tgydF4y2Bad> <td align="center"><span style="width: 57.424999px;"> <svg height="11.1" id="M12" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 57.424999 11.1" width="57.424999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.1)"> <g transform="translate(72,-63.12)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="39.584499" y="0"> 3</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">150525 . . 150184</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> </tr> <tr> <td align="left">YGL189C</tgydF4y2Bad> <td align="center">RPS26A</tgydF4y2Bad> <td align="center">7</tgydF4y2Bad> <td align="center"><span style="width: 57.424999px;"> <svg height="10.9125" id="M13" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 57.424999 10.9125" width="57.424999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.9125)"> <g transform="translate(72,-63.27)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 7</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 8</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="39.584499" y="0"> 1</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">148966 . . 148599</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> </tr> <tr> <td align="left">YGR027C</tgydF4y2Bad> <td align="center">RPS25A</tgydF4y2Bad> <td align="center">7</tgydF4y2Bad> <td align="center"><span style="width: 57.424999px;"> <svg height="11.0375" id="M14" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 57.424999 11.0375" width="57.424999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0375)"> <g transform="translate(72,-63.17)"> <text transform="matrix(1,0,0,-1,-71.95,63.4)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 2</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 7</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="39.584499" y="0"> 6</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">534785 . . 534474</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> </tr> <tr> <td align="left">YGR148C</tgydF4y2Bad> <td align="center">RPL24B</tgydF4y2Bad> <td align="center">7</tgydF4y2Bad> <td align="center"><span style="width: 49.599998px;"> <svg height="11.0375" id="M15" style="vertical-align:-0.27588pt" version="1.1" viewbox="0 0 49.599998 11.0375" width="49.599998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0375)"> <g transform="translate(72,-63.17)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 9</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 9</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 8</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">788178 . . 787787</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> </tr> <tr> <td align="left">YIL123W</tgydF4y2Bad> <td align="center">SIM1</tgydF4y2Bad> <td align="center">9</tgydF4y2Bad> <td align="center"><span style="width: 49.599998px;"> <svg height="11.0375" id="M16" style="vertical-align:-0.27588pt" version="1.1" viewbox="0 0 49.599998 11.0375" width="49.599998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0375)"> <g transform="translate(72,-63.17)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 8</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 9</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 3</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">127662 . . 128148</tgydF4y2Bad> <td align="center">W</tgydF4y2Bad> </tr> <tr> <td align="left">YJL130C</tgydF4y2Bad> <td align="center">URA2</tgydF4y2Bad> <td align="center">10</tgydF4y2Bad> <td align="center"><span style="width: 57.424999px;"> <svg height="11.1" id="M17" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 57.424999 11.1" width="57.424999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.1)"> <g transform="translate(72,-63.12)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 8</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 6</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="39.584499" y="0"> 6</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">172752 . . 172433</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> </tr> <tr> <td align="left">YKL150W</tgydF4y2Bad> <td align="center">MCR1</tgydF4y2Bad> <td align="center">11</tgydF4y2Bad> <td align="center"><span style="width: 57.424999px;"> <svg height="11.1" id="M18" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 57.424999 11.1" width="57.424999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.1)"> <g transform="translate(72,-63.12)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="39.584499" y="0"> 7</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">166400 . . 166487</tgydF4y2Bad> <td align="center">W</tgydF4y2Bad> </tr> <tr> <td align="left">YKL186C</tgydF4y2Bad> <td align="center">MTR2</tgydF4y2Bad> <td align="center">11</tgydF4y2Bad> <td align="center"><span style="width: 57.424999px;"> <svg height="11.0375" id="M19" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 57.424999 11.0375" width="57.424999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0375)"> <g transform="translate(72,-63.17)"> <text transform="matrix(1,0,0,-1,-71.95,63.4)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 6</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 7</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="39.584499" y="0"> 4</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">93465 . . 93312</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> </tr> <tr> <td align="left">YLR333C</tgydF4y2Bad> <td align="center">RPS25B</tgydF4y2Bad> <td align="center">12</tgydF4y2Bad> <td align="center"><span style="width: 57.424999px;"> <svg height="11.0375" id="M20" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 57.424999 11.0375" width="57.424999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0375)"> <g transform="translate(72,-63.17)"> <text transform="matrix(1,0,0,-1,-71.95,63.4)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 6</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="39.584499" y="0"> 4</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">796335 . . 795913</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> </tr> <tr> <td align="left">YLR367W</tgydF4y2Bad> <td align="center">RPS22B</tgydF4y2Bad> <td align="center">12</tgydF4y2Bad> <td align="center"><span style="width: 49.599998px;"> <svg height="11.1" id="M21" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 49.599998 11.1" width="49.599998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.1)"> <g transform="translate(72,-63.12)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 6</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 8</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">855878 . . 856434</tgydF4y2Bad> <td align="center">W</tgydF4y2Bad> </tr> <tr> <td align="left">YLR388W</tgydF4y2Bad> <td align="center">RPS29A</tgydF4y2Bad> <td align="center">12</tgydF4y2Bad> <td align="center"><span style="width: 49.599998px;"> <svg height="11.1625" id="M22" style="vertical-align:-0.27588pt" version="1.1" viewbox="0 0 49.599998 11.1625" width="49.599998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.1625)"> <g transform="translate(72,-63.07)"> <text transform="matrix(1,0,0,-1,-71.95,63.4)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 9</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 6</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">898158 . . 898645</tgydF4y2Bad> <td align="center">W</tgydF4y2Bad> </tr> <tr> <td align="left">YNL066W</tgydF4y2Bad> <td align="center">SUN4</tgydF4y2Bad> <td align="center">14</tgydF4y2Bad> <td align="center"><span style="width: 57.424999px;"> <svg height="11.1" id="M23" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 57.424999 11.1" width="57.424999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.1)"> <g transform="translate(72,-63.12)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 8</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.043749" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.169498" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="39.584499" y="0"> 3</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">501157 . . 501502</tgydF4y2Bad> <td align="center">W</tgydF4y2Bad> </tr> <tr> <td align="left">YPL230W</tgydF4y2Bad> <td align="center">USV1</tgydF4y2Bad> <td align="center">16</tgydF4y2Bad> <td align="center"><span style="width: 49.599998px;"> <svg height="11.0375" id="M24" style="vertical-align:-0.27588pt" version="1.1" viewbox="0 0 49.599998 11.0375" width="49.599998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0375)"> <g transform="translate(72,-63.17)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> 9</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.414999" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.666498" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="19.79225" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.917999" y="0"> - - - - - -</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="27.081497" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.332996" y="0"> 9</tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">115219 . . 115293</tgydF4y2Bad> <td align="center">W</tgydF4y2Bad> </tr> <tr class="table-tr"> <td colspan="6"> <hr class="tbody-hr"></td> </tr> </tbody> </table></td> </tr> <tr class="table-fn"> <td><sup>(1)</gydF4y2Basup>系统的名称。<gydF4y2Babr><sup>(2)</gydF4y2Basup>标准名称。<gydF4y2Babr><sup>(3)</gydF4y2Basup>相对于启动密码子站点编号。<gydF4y2Babr><sup>(4)</gydF4y2Basup>C-Crick链(反向互补),W-Watson链。<gydF4y2Babr></td> </tr> </tbody> </table> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">表1</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/tab1/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> 的名字和内含子位置24酵母蛋白编码基因中内含子5′-UTRs。</gydF4y2Badiv> </div> </div> <p>对于每个基因内区,我们最初提取10基地从外显子和12基地从基因内区采用DAMBE [<一个href="#B41">41</一个>,<gydF4y2Ba一个href="#B42">42</一个>]。这<gydF4y2Basvg height="11.325" id="M25" style="vertical-align:-0.51414pt;width:49.037498px;" version="1.1" viewbox="0 0 49.037498 11.325" width="49.037498" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.325)"> <g transform="translate(72,-62.94)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="6.2515001" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="15.278666" y="0"> +</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.618887" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="32.870388" y="0"> 2</tgydF4y2Baspan> </text> </g> </g> </svg>配置排除了一些学生因为一些酵母基因第一外显子是短于10基地(注意,“第一外显子”是指第一个外显子编码的部分摘要)。例如,第一个外显子的two-exon<我>MUD1</gydF4y2Ba我>基因只有八个基地长。因为我们的提取需要10基地外显子,这样的基因5′党卫军将错过。最极端的情况下的<我>RPL20A</gydF4y2Ba我>和<我>RPL20B</gydF4y2Ba我>基因单核苷酸作为第一外显子,也就是说,在配置5′-∣基因内区∣TG-3′。的要求<gydF4y2Basvg height="11.325" id="M26" style="vertical-align:-0.51414pt;width:49.037498px;" version="1.1" viewbox="0 0 49.037498 11.325" width="49.037498" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.325)"> <g transform="translate(72,-62.94)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="6.2515001" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="15.278666" y="0"> +</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.618887" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="32.870388" y="0"> 2</tgydF4y2Baspan> </text> </g> </g> </svg>配置,5′党卫军的总数只有223在酵母基因组中。初步分析表明,只有网站的外显子5′党卫军显示显著的序列保护,我们定义5′ss由外显子5核苷酸的网站和12个核苷酸基因内区一侧(以下称为<gydF4y2Basvg height="11.5125" id="M27" style="vertical-align:-0.51414pt;width:41.224998px;" version="1.1" viewbox="0 0 41.224998 11.5125" width="41.224998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.5125)"> <g transform="translate(72,-62.79)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="9.0271664" y="0"> +</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="20.367388" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.618887" y="0"> 2</tgydF4y2Baspan> </text> </g> </g> </svg>配置)。同样,一个3′党卫军由12个核苷酸的内含子和外显子5核苷酸。结果275年5′ss和301 3′的党卫军<gydF4y2Basvg height="11.5125" id="M28" style="vertical-align:-0.51414pt;width:41.224998px;" version="1.1" viewbox="0 0 41.224998 11.5125" width="41.224998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.5125)"> <g transform="translate(72,-62.79)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="9.0271664" y="0"> +</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="20.367388" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.618887" y="0"> 2</tgydF4y2Baspan> </text> </g> </g> </svg>配置,包括在5′UTR 24个内含子。</gydF4y2Bap> <p>一些研究人员(例如,<一个href="#B39">39</一个>,<gydF4y2Ba一个href="#B43">43</一个>])已经5′学生由过去的3外显子的核苷酸的第一个6或7核苷酸基因内区。5′党卫军中定义这种方式可能会产生虚假的网站模式的酵母。例如,如表所示<一个href="//www.newsama.com/journals/ijg/2011/212146/tab2/" target="_blank">2</一个>列表基因的5′党卫军排除由于上游外显子太短,20吗<我>酿酒酵母</我>基因第一外显子三个核苷酸(即。,只包含起始密码子)。定义5′ss和三个核苷酸的外显子一边将大幅增加的表示,U,三个核苷酸网站(即和G。−−3,2−1网站)5′ss(第一个内含子的核苷酸是标记为1)。</gydF4y2Bap> <div class="floats-partial-section partial-other-wrapper" data-section="tab2"> <div class="floats-partial-content fig-content-tab2"> <table class="table-group" id="tab2"> <tbody> <tr> <td> <table class="table"> <tbody> <tr> <td class="thead-hr" colspan="4"> <hr></td> </tr> <tr class="thead"> <td align="left">基因</tgydF4y2Bad> <td align="center">PWM *</tgydF4y2Bad> <td align="center">第一外显子兰</tgydF4y2Bad> <td align="center">序列</tgydF4y2Bad> </tr> <tr> <td class="thead-hr" colspan="4"> <hr></td> </tr> <tr> <td align="left">BET4</tgydF4y2Bad> <td align="center">4.2977</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">BOS1</tgydF4y2Bad> <td align="center">3.5760</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">DCN1</tgydF4y2Bad> <td align="center">6.5363</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">MND1</tgydF4y2Bad> <td align="center">8.1685</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">MPT5</tgydF4y2Bad> <td align="center">8.5055</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">PSP2</tgydF4y2Bad> <td align="center">8.4546</tgydF4y2Bad> <td align="center">4</tgydF4y2Bad> <td align="center">AUGG</tgydF4y2Bad> </tr> <tr> <td align="left">QCR9</tgydF4y2Bad> <td align="center">5.7592</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPL13A</tgydF4y2Bad> <td align="center">6.9991</tgydF4y2Bad> <td align="center">4</tgydF4y2Bad> <td align="center">AUGG</tgydF4y2Bad> </tr> <tr> <td align="left">RPL13B</tgydF4y2Bad> <td align="center">8.6752</tgydF4y2Bad> <td align="center">4</tgydF4y2Bad> <td align="center">AUGG</tgydF4y2Bad> </tr> <tr> <td align="left">RPL19A</tgydF4y2Bad> <td align="center">6.9298</tgydF4y2Bad> <td align="center">2</tgydF4y2Bad> <td align="center">非盟</tgydF4y2Bad> </tr> <tr> <td align="left">RPL19B</tgydF4y2Bad> <td align="center">11.7762</tgydF4y2Bad> <td align="center">2</tgydF4y2Bad> <td align="center">非盟</tgydF4y2Bad> </tr> <tr> <td align="left">RPL20A</tgydF4y2Bad> <td align="center">9.7145</tgydF4y2Bad> <td align="center">1</tgydF4y2Bad> <td align="center">一个</tgydF4y2Bad> </tr> <tr> <td align="left">RPL20B</tgydF4y2Bad> <td align="center">8.0214</tgydF4y2Bad> <td align="center">1</tgydF4y2Bad> <td align="center">一个</tgydF4y2Bad> </tr> <tr> <td align="left">RPL2A</tgydF4y2Bad> <td align="center">12.0769</tgydF4y2Bad> <td align="center">4</tgydF4y2Bad> <td align="center">AUGG</tgydF4y2Bad> </tr> <tr> <td align="left">RPL2B</tgydF4y2Bad> <td align="center">9.7326</tgydF4y2Bad> <td align="center">4</tgydF4y2Bad> <td align="center">AUGG</tgydF4y2Bad> </tr> <tr> <td align="left">RPL30</tgydF4y2Bad> <td align="center">8.1799</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPL35A</tgydF4y2Bad> <td align="center">7.3834</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPL35B</tgydF4y2Bad> <td align="center">7.8326</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPL42A</tgydF4y2Bad> <td align="center">9.2392</tgydF4y2Bad> <td align="center">4</tgydF4y2Bad> <td align="center">AUGG</tgydF4y2Bad> </tr> <tr> <td align="left">RPL42B</tgydF4y2Bad> <td align="center">7.0558</tgydF4y2Bad> <td align="center">4</tgydF4y2Bad> <td align="center">AUGG</tgydF4y2Bad> </tr> <tr> <td align="left">RPL43A</tgydF4y2Bad> <td align="center">9.9976</tgydF4y2Bad> <td align="center">2</tgydF4y2Bad> <td align="center">非盟</tgydF4y2Bad> </tr> <tr> <td align="left">RPL43B</tgydF4y2Bad> <td align="center">12.0547</tgydF4y2Bad> <td align="center">2</tgydF4y2Bad> <td align="center">非盟</tgydF4y2Bad> </tr> <tr> <td align="left">RPS17A</tgydF4y2Bad> <td align="center">9.1269</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPS17B</tgydF4y2Bad> <td align="center">10.1283</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPS24A</tgydF4y2Bad> <td align="center">9.4227</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPS24B</tgydF4y2Bad> <td align="center">11.3548</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPS27A</tgydF4y2Bad> <td align="center">6.3612</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPS27B</tgydF4y2Bad> <td align="center">10.3823</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPS30A</tgydF4y2Bad> <td align="center">10.8845</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">RPS30B</tgydF4y2Bad> <td align="center">6.2290</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">UBC12</tgydF4y2Bad> <td align="center">8.4505</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">VMA10</tgydF4y2Bad> <td align="center">8.2722</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr> <td align="left">YSF3</tgydF4y2Bad> <td align="center">7.1596</tgydF4y2Bad> <td align="center">3</tgydF4y2Bad> <td align="center">8月</tgydF4y2Bad> </tr> <tr class="table-tr"> <td colspan="4"> <hr class="tbody-hr"></td> </tr> </tbody> </table></td> </tr> <tr class="table-fn"> <td>*位置权重矩阵得分在3′党卫军。<gydF4y2Babr></td> </tr> </tbody> </table> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">表2</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/tab2/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> 酵母基因第一外显子(即。,the coding part of the first exon) is shorter than five nucleotides.</div> </div> </div> <p>一些酵母内含子可能是标注错误。带注释的基因内区<我>YJR112W-A</我>在酵母基因是最短的基因内区(49 bp)和AG)并未结束。基因内区实际上是不再可能与真正的3′ss下游。根据SGD注释(<一个href="#B44">44</一个>),<gydF4y2Ba我>YJR112W-A</我>被描述为“假定的未知蛋白质的功能,确定了基于同源性<我>一个shbya棉。</我>“因此,我们将其3′ss排除在我们的分析。这减少了303 3′ss 302。</gydF4y2Bap> <h5 id="sec2.2">2.2。描述拼接的效率网站(ss)位置权重矩阵(麻省)和序列标识</h5><gydF4y2Bap>共识5′ss基因内区一侧GUAUGU酵母。因此,一个简单的方法来描述5′ss拼接的力量会给5′党卫军接合强度高值如果是类似低价值共识,但如果是完全不同的共识。更正式的方法是描述纳粹党卫军的位置权重矩阵(PWM, (<一个href="#B37">37</一个>,<gydF4y2Ba一个href="#B38">38</一个>]的详细数值说明)并使用脉宽调制(PWM)得分为每个学生作为其接合强度指数(<一个href="#B39">39</一个>,<gydF4y2Ba一个href="#B40">40</一个>]。我们使用DAMBE [<一个href="#B41">41</一个>,<gydF4y2Ba一个href="#B42">42</一个>)来计算pwm。</gydF4y2Bap> <p>整个记录的核苷酸频率(即。,我ncluding both exons and introns) were used as background frequencies for computing PWM, with A<svg height="6.1624999" id="M29" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.3279,C<gydF4y2Basvg height="6.1624999" id="M30" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.1915 G<gydF4y2Basvg height="6.1624999" id="M31" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.2043,你<gydF4y2Basvg height="6.1624999" id="M32" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.2763。因为一些特定站点的频率是0,pseudocount<gydF4y2Basvg height="10.9125" id="M33" style="vertical-align:-0.17555pt;width:71.3125px;" version="1.1" viewbox="0 0 71.3125 10.9125" width="71.3125" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.9125)"> <g transform="translate(72,-63.27)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.515023" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.555412" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="28.806911" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="31.932661" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="38.184162" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="44.435661" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="50.68716" y="0"> 1</tgydF4y2Baspan> </text> </g> </g> </svg>添加到所有频率来避免日志吗<gydF4y2Basub>2</gydF4y2Basub>(0)(<一个href="#B38">38</一个>,83- - - - - -92年页)。另一种是指定所有外显子的核苷酸频率频率为背景的外显子部分内含子的ss和核苷酸频率为背景频率的基因内区党卫军的一部分。然而,结果因此获得类似于那些使用第一种方法。我们也结果通过核苷酸频率的频率提取的ss作为背景。结果相似。</gydF4y2Bap> <p>几项研究[<一个href="#B43">43</一个>,<gydF4y2Ba一个href="#B45">45</一个>)认为平等背景频率特征与PWM党卫军。这不是一种好方法,因为它混淆站点特定的核苷酸偏见在党卫军基因组核苷酸的偏见。例如,酵母基因组是丰富的,随机的序列片段组装AT富集核苷酸池也将在丰富。这样的随机部分,以PWM频率相等的背景时,会出现信息并导致错误发现网站的模式。</gydF4y2Bap> <p>图形化显示特定核苷酸模式的另一个常用的方法是序列标识已被用来描述基因内区ss (<一个href="#B19">19</一个>]。原来的方法(<一个href="#B46">46</一个>)不考虑背景核苷酸的偏见,以及由此产生的序列标识相当于一个PWM假设核苷酸频率相等。例如,有偏背景频率酵母暗示标志序列将显示比C和G和T更加突出甚至当感兴趣的序列不包含特定站点的信息。然而,这个问题已经被最近的改进(<一个href="#B47">47</一个>),允许一个指定背景(之前)就像在PWM频率。摘要语标序列生成的RNA结构标识网站<一个href="http://www.cbs.dtu.dk/~gorodkin/appl/slogo.html" target="_blank">http://www.cbs.dtu.dk/ gorodkin /: / slogo.html</一个>。</gydF4y2Bap> <h5 id="sec2.3">2.3。基因表达</h5><gydF4y2Bap>我们使用了基因表达的三个措施。第一个是密码子适应指数(<一个href="#B48">48</一个>在DAMBE[]的改进实现<一个href="#B49">49</一个>),计算高度的参考集表示酵母基因的密码子使用表Eysc_h编译。减少文件分布式浮雕(<一个href="#B50">50</一个>]。编码序列(CDSs)计算CAI使用DAMBE提取。CAI的目的是衡量翻译伸长但高度的效率和呈正相关基因表达的蛋白质和mRNA水平(<一个href="#B51">51</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B53">53</一个>]。使用CAI的优势是它可以计算为所有编码序列,而实证量化的相对高度表达基因的基因表达可能是有限的,不会给我们一个整体的照片拼接的力量和基因表达之间的关系。</gydF4y2Bap> <p>第二个衡量基因表达的是酵母基因的mRNA相对丰度从两个先前的研究,描述在酵母全基因组RNA丰度(<一个href="#B27">27</一个>,<gydF4y2Ba一个href="#B28">28</一个>]。微阵列数据(<一个href="#B27">27</一个>)下载<一个href="http://web.wi.mit.edu/young/pub/data/orf_transcriptome.txt" target="_blank">http://web.wi.mit.edu/young/pub/data/orf_transcriptome.txt</一个>。数据集包括5460个酵母基因的mRNA水平。绝对量化数据(<一个href="#B28">28</一个>从网上下载补充材料。只有4817个基因的平均表达YPD介质进行了分析。</gydF4y2Bap> <p>第三测量基因表达的蛋白质生产的酵母基因特征在两个先前的研究。蛋白质丰度数据<一个href="#B29">29日</一个>)下载<一个href="http://www.nature.com/nature/journal/v425/n6959/extref/nature02046-s2.xls" target="_blank">http://www.nature.com/nature/journal/v425/n6959/extref/nature02046-s2.xls”> nature02046-s2.xls</一个>。预测蛋白质合成率在两个实验条件(交配信息素的治疗和控制)可靠地测量了3916个基因(补充表二世(<一个href="#B30">30.</一个>]),我们使用两个实验的平均值。</gydF4y2Bap> <p>信使rna和蛋白质的表征,YAR044W同义YAR042W基因库的文件,所以YDR474C YDR475C, YJL018W YJL019W, YJL021C YJL020C, YPR090W YPR089W, YFR024C YFR024C-A。一些基因(YEL068C YER084W、YHR173C YIL054W, YJR146W, YLR358C, YNL140C, YNL143C, YNL184C,和YOR105W)在SGD注释“可疑的开放阅读框可能编码一种蛋白质”,并没有注释的<我>酿酒酵母</我>在NCBI基因组。然而,他们被发现在mRNA表达(<一个href="#B27">27</一个>和蛋白质含量<一个href="#B29">29日</一个>),因此在我们的分析中。YFL006W和YFL007W并入YFL007W, YJL017W YJL016W成YJL016W, YOR087W和YOR088W YOR087W在最近的酵母基因组注释。</gydF4y2Bap> <p>两个数据文件编译连接作为补充材料。一个(PWM-All.xls)包括所有内含子、mRNA丰富从GATC-PCR方法<一个href="#B28">28</一个>),基于核糖体加载和信使rna和蛋白质合成率(<一个href="#B30">30.</一个>]。其他(PWM-No5UTRintrno.xls)不包括5′UTR内含子,包括mRNA丰富从微阵列<一个href="#B27">27</一个>)和蛋白质丰度数据(<一个href="#B29">29日</一个>]。</gydF4y2Bap> <h4 id="results-and-discussion">3所示。结果与讨论</h4><h5gydF4y2Baid="sec3.1">3.1。位置权重矩阵(PWM)及其统计意义</h5><gydF4y2Bap>符合之前的实验研究<我>酿酒酵母</我>位置权重矩阵(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab3/" target="_blank">3</一个>和<一个href="//www.newsama.com/journals/ijg/2011/212146/tab4/" target="_blank">4</一个>标识(图)和序列<一个href="//www.newsama.com/journals/ijg/2011/212146/fig1/" target="_blank">1</一个>)不仅证实,也扩大了酵母的共识序列拼接网站,与UAAAG∣GUAUGUUUAAUU最强的5′ss和UUUUUUUUAYAG∣GCUUC最强的3′党卫军。PWM包含重要的特定站点信息是否可以测试通过<gydF4y2Basvg height="10.325" id="M34" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>统计(<一个href="#B37">37</一个>)定义为<gydF4y2Baspan class="equation" id="EEq1"> <svg height="52.849998" id="M35" preserveaspectratio="xMaxYMin" style="vertical-align:-0pt;width:700.9375px;" version="1.1" viewbox="0 0 700.9375 52.849998" width="700.9375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,52.85)"> <g transform="translate(72,-29.72)"> <text transform="matrix(1,0,0,-1,156.59,47.62)"> <tspan style="font-size: 12.50px; " x="0" y="0"> 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transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>是序列长度(在我们的研究主题宽度等于17),<gydF4y2Basvg height="12.8875" id="M37" style="vertical-align:-1.76814pt;width:71.262497px;" version="1.1" viewbox="0 0 71.262497 12.8875" width="71.262497" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,12.8875)"> <g transform="translate(72,-61.69)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="7.3517642" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="19.392153" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="25.643654" y="0"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="35.020905" y="0"> 2</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="41.272404" y="0"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="50.649654" y="0"> 3</tgydF4y2Baspan> </text> </g> </g> </svg>和4对应A、C、G和U,<gydF4y2Basvg height="11.05" id="M38" style="vertical-align:-3.2316pt;width:11.7875px;" version="1.1" viewbox="0 0 11.7875 11.05" width="11.7875" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.05)"> <g transform="translate(72,-63.16)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-65.84,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>核苷酸背景核苷酸频率吗<gydF4y2Basvg height="9.9250002" id="M39" style="vertical-align:-0.13794pt;width:4.9875002px;" version="1.1" viewbox="0 0 4.9875002 9.9250002" width="4.9875002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.925)"> <g transform="translate(72,-64.06)"> <text transform="matrix(1,0,0,-1,-71.95,64.25)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>,<gydF4y2Basvg height="12.975" id="M40" style="vertical-align:-4.77652pt;width:16.700001px;" version="1.1" viewbox="0 0 16.700001 12.975" width="16.700001" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,12.975)"> <g transform="translate(72,-61.62)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-65.84,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.7218721" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>核苷酸的频率<gydF4y2Basvg height="9.9250002" id="M41" style="vertical-align:-0.13794pt;width:4.9875002px;" version="1.1" viewbox="0 0 4.9875002 9.9250002" width="4.9875002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.925)"> <g transform="translate(72,-64.06)"> <text transform="matrix(1,0,0,-1,-71.95,64.25)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>在位置<gydF4y2Basvg height="13.6125" id="M42" style="vertical-align:-2.34499pt;width:100.6875px;" version="1.1" viewbox="0 0 100.6875 13.6125" width="100.6875" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,13.6125)"> <g transform="translate(72,-61.11)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.863667" y="0"> (</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="9.0271664" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="21.055052" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="27.306553" y="0"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="32.520302" y="0"> 2</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="38.771805" y="0"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="43.985554" y="0"> …</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="58.564053" y="0"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="63.777802" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="70.029305" y="0"> 7</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="76.2808" y="0"> )</tgydF4y2Baspan> </text> </g> </g> </svg>。一个简单的方法来评估PWM的重要性重采样。定义的tetranomial分布<gydF4y2Basvg height="17.7875" id="M43" style="vertical-align:-3.25792pt;width:134.7px;" version="1.1" viewbox="0 0 134.7 17.7875" width="134.7" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,17.7875)"> <g transform="translate(72,-57.77)"> <text transform="matrix(1,0,0,-1,-71.95,61.07)"> <tspan style="font-size: 12.50px; " x="0" y="0"> (</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-61.67,57.94)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 一个</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-52.08,61.07)"> <tspan style="font-size: 12.50px; " x="0" y="0"> +</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="11.340221" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-34.62,57.94)"> <tspan style="font-size: 8.75px; " x="0" y="0"> C</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-25.51,61.07)"> <tspan style="font-size: 12.50px; " x="0" y="0"> +</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="11.340221" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-8.05,57.94)"> <tspan style="font-size: 8.75px; " x="0" y="0"> G</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,1.55,61.07)"> <tspan style="font-size: 12.50px; " x="0" y="0"> +</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="11.327718" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,19,57.94)"> <tspan style="font-size: 8.75px; " x="0" y="0"> T</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,24.85,61.07)"> <tspan style="font-size: 12.50px; " x="0" y="0"> )</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,29.01,66.24)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>,我们可以得到一组新的序列(例如,246的17元序列)和计算<gydF4y2Basvg height="10.325" id="M44" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>。这是重复的,获得5000年的5000倍<gydF4y2Basvg height="10.325" id="M45" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>值。的99或第95百分位<gydF4y2Basvg height="10.325" id="M46" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>值可以作为至关重要的<gydF4y2Basvg height="10.325" id="M47" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>值分别为0.05和0.01的水平,意义。一个观察<gydF4y2Basvg height="10.325" id="M48" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>PWM的显著大于临界<gydF4y2Basvg height="10.325" id="M49" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>。根据这一标准,PWM从275年从301 3′5′ss和学生都是非常重要的(<gydF4y2Basvg height="11.0625" id="M50" style="vertical-align:-0.30096pt;width:71.775002px;" version="1.1" viewbox="0 0 71.775002 11.0625" width="71.775002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0625)"> <g transform="translate(72,-63.15)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.182001" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="38.559254" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="44.810753" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="51.062252" y="0"> 1</tgydF4y2Baspan> </text> </g> </g> </svg>)。它也是非常重要的(<gydF4y2Basvg height="11.0625" id="M51" style="vertical-align:-0.30096pt;width:71.775002px;" version="1.1" viewbox="0 0 71.775002 11.0625" width="71.775002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0625)"> <g transform="translate(72,-63.15)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.182001" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="38.559254" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="44.810753" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="51.062252" y="0"> 1</tgydF4y2Baspan> </text> </g> </g> </svg>)24内含子5′UTR被排除在外。</gydF4y2Bap> <div class="floats-partial-section partial-other-wrapper" data-section="tab3"> <div class="floats-partial-content fig-content-tab3"> <table class="table-group" id="tab3"> <tbody> <tr> <td> <table class="table"> <tbody> <tr> <td class="thead-hr" colspan="11"> <hr></td> </tr> <tr class="thead"> <td align="left">网站</tgydF4y2Bad> <td align="center">一个</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> <td align="center">G</tgydF4y2Bad> <td align="center">U</tgydF4y2Bad> <td align="center"><i>χ</我><sup>2</gydF4y2Basup></td> <td align="center"><span style="width: 11.75px;"> <svg height="10.325" id="M56" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">一个</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> <td align="center">G</tgydF4y2Bad> <td align="center">U</tgydF4y2Bad> </tr> <tr> <td class="thead-hr" colspan="11"> <hr></td> </tr> <tr> <td align="left">−5</tgydF4y2Bad> <td align="center">94年</tgydF4y2Bad> <td align="center">32</tgydF4y2Bad> <td align="center">57</tgydF4y2Bad> <td align="center">92年</tgydF4y2Bad> <td align="center">11.798</tgydF4y2Bad> <td align="center">0.0081088</tgydF4y2Bad> <td align="center">0.0641</tgydF4y2Bad> <td align="center">−0.7117</tgydF4y2Bad> <td align="center">0.0245</tgydF4y2Bad> <td align="center">0.2792</tgydF4y2Bad> </tr> <tr> <td align="left">−4</tgydF4y2Bad> <td align="center">119年</tgydF4y2Bad> <td align="center">47</tgydF4y2Bad> <td align="center">48</tgydF4y2Bad> <td align="center">61年</tgydF4y2Bad> <td align="center">14.117</tgydF4y2Bad> <td align="center">0.0027505</tgydF4y2Bad> <td align="center">0.4032</tgydF4y2Bad> <td align="center">−0.1599</tgydF4y2Bad> <td align="center">−0.2225</tgydF4y2Bad> <td align="center">−0.3115</tgydF4y2Bad> </tr> <tr> <td align="left">−3</tgydF4y2Bad> <td align="center">139年</tgydF4y2Bad> <td align="center">38</tgydF4y2Bad> <td align="center">43</tgydF4y2Bad> <td align="center">55</tgydF4y2Bad> <td align="center">39.672</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center"><b><i>0.6268</我></b></td> <td align="center">−0.4651</tgydF4y2Bad> <td align="center">−0.3805</tgydF4y2Bad> <td align="center">−0.4601</tgydF4y2Bad> </tr> <tr> <td align="left">−2</tgydF4y2Bad> <td align="center">138年</tgydF4y2Bad> <td align="center">40</tgydF4y2Bad> <td align="center">36</tgydF4y2Bad> <td align="center">61年</tgydF4y2Bad> <td align="center">38.899</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center"><b><i>0.6164</我></b></td> <td align="center">−0.3915</tgydF4y2Bad> <td align="center">−0.6355</tgydF4y2Bad> <td align="center">−0.3115</tgydF4y2Bad> </tr> <tr> <td align="left">−1</tgydF4y2Bad> <td align="center">91年</tgydF4y2Bad> <td align="center">45</tgydF4y2Bad> <td align="center">88年</tgydF4y2Bad> <td align="center">51</tgydF4y2Bad> <td align="center">27.270</tgydF4y2Bad> <td align="center">0.0000052</tgydF4y2Bad> <td align="center">0.0174</tgydF4y2Bad> <td align="center">−0.2223</tgydF4y2Bad> <td align="center"><b><i>0.6492</我></b></td> <td align="center">−0.5685</tgydF4y2Bad> </tr> <tr> <td align="left">1</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">1</tgydF4y2Bad> <td align="center">274年</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">1060.426</tgydF4y2Bad> <td align="center">0.0000004</tgydF4y2Bad> <td align="center">−8.1042</tgydF4y2Bad> <td align="center">−5.4675</tgydF4y2Bad> <td align="center"><b><i>2.2855</我></b></td> <td align="center">−8.1044</tgydF4y2Bad> </tr> <tr> <td align="left">2</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">9</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">266年</tgydF4y2Bad> <td align="center">658.096</tgydF4y2Bad> <td align="center">0.0000003</tgydF4y2Bad> <td align="center">−8.1042</tgydF4y2Bad> <td align="center">−2.5200</tgydF4y2Bad> <td align="center">−8.1048</tgydF4y2Bad> <td align="center"><b><i>1.8081</我></b></td> </tr> <tr> <td align="left">3</tgydF4y2Bad> <td align="center">268年</tgydF4y2Bad> <td align="center">1</tgydF4y2Bad> <td align="center">2</tgydF4y2Bad> <td align="center">4</tgydF4y2Bad> <td align="center">522.754</tgydF4y2Bad> <td align="center">0.0000003</tgydF4y2Bad> <td align="center"><b><i>1.5723</我></b></td> <td align="center">−5.4675</tgydF4y2Bad> <td align="center">−4.6732</tgydF4y2Bad> <td align="center">−4.1523</tgydF4y2Bad> </tr> <tr> <td align="left">4</tgydF4y2Bad> <td align="center">17</tgydF4y2Bad> <td align="center">29日</tgydF4y2Bad> <td align="center">1</tgydF4y2Bad> <td align="center">228年</tgydF4y2Bad> <td align="center">428.607</tgydF4y2Bad> <td align="center">0.0000002</tgydF4y2Bad> <td align="center">−2.3805</tgydF4y2Bad> <td align="center">−0.8528</tgydF4y2Bad> <td align="center">−5.5454</tgydF4y2Bad> <td align="center"><b><i>1.5859</我></b></td> </tr> <tr> <td align="left">5</tgydF4y2Bad> <td align="center">2</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">272年</tgydF4y2Bad> <td align="center">1</tgydF4y2Bad> <td align="center">1041.047</tgydF4y2Bad> <td align="center">0.0000004</tgydF4y2Bad> <td align="center">−5.2765</tgydF4y2Bad> <td align="center">−8.1049</tgydF4y2Bad> <td align="center"><b><i>2.2750</我></b></td> <td align="center">−5.8967</tgydF4y2Bad> </tr> <tr> <td align="left">6</tgydF4y2Bad> <td align="center">10</tgydF4y2Bad> <td align="center">8</tgydF4y2Bad> <td align="center">2</tgydF4y2Bad> <td align="center">255年</tgydF4y2Bad> <td align="center">583.545</tgydF4y2Bad> <td align="center">0.0000003</tgydF4y2Bad> <td align="center">−3.1271</tgydF4y2Bad> <td align="center">−2.6862</tgydF4y2Bad> <td align="center">−4.6732</tgydF4y2Bad> <td align="center"><b><i>1.7472</我></b></td> </tr> <tr> <td align="left">7</tgydF4y2Bad> <td align="center">97年</tgydF4y2Bad> <td align="center">18</tgydF4y2Bad> <td align="center">39</tgydF4y2Bad> <td align="center">121年</tgydF4y2Bad> <td align="center">55.570</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center">0.1092</tgydF4y2Bad> <td align="center">−1.5351</tgydF4y2Bad> <td align="center">−0.5206</tgydF4y2Bad> <td align="center"><b><i>0.6734</我></b></td> </tr> <tr> <td align="left">8</tgydF4y2Bad> <td align="center">95年</tgydF4y2Bad> <td align="center">54</tgydF4y2Bad> <td align="center">35</tgydF4y2Bad> <td align="center">91年</tgydF4y2Bad> <td align="center">11.363</tgydF4y2Bad> <td align="center">0.0099180</tgydF4y2Bad> <td align="center">0.0793</tgydF4y2Bad> <td align="center">0.0397</tgydF4y2Bad> <td align="center">−0.6759</tgydF4y2Bad> <td align="center">0.2635</tgydF4y2Bad> </tr> <tr> <td align="left">9</tgydF4y2Bad> <td align="center">123年</tgydF4y2Bad> <td align="center">45</tgydF4y2Bad> <td align="center">34</tgydF4y2Bad> <td align="center">73年</tgydF4y2Bad> <td align="center">22.172</tgydF4y2Bad> <td align="center">0.0000601</tgydF4y2Bad> <td align="center">0.4508</tgydF4y2Bad> <td align="center">−0.2223</tgydF4y2Bad> <td align="center">−0.7175</tgydF4y2Bad> <td align="center">−0.0534</tgydF4y2Bad> </tr> <tr> <td align="left">10</tgydF4y2Bad> <td align="center">118年</tgydF4y2Bad> <td align="center">41</tgydF4y2Bad> <td align="center">38</tgydF4y2Bad> <td align="center">78年</tgydF4y2Bad> <td align="center">17.334</tgydF4y2Bad> <td align="center">0.0006034</tgydF4y2Bad> <td align="center">0.3911</tgydF4y2Bad> <td align="center">−0.3560</tgydF4y2Bad> <td align="center">−0.5579</tgydF4y2Bad> <td align="center">0.0418</tgydF4y2Bad> </tr> <tr> <td align="left">11</tgydF4y2Bad> <td align="center">105年</tgydF4y2Bad> <td align="center">33</tgydF4y2Bad> <td align="center">43</tgydF4y2Bad> <td align="center">94年</tgydF4y2Bad> <td align="center">17.367</tgydF4y2Bad> <td align="center">0.0005940</tgydF4y2Bad> <td align="center">0.2232</tgydF4y2Bad> <td align="center">−0.6676</tgydF4y2Bad> <td align="center">−0.3805</tgydF4y2Bad> <td align="center">0.3101</tgydF4y2Bad> </tr> <tr> <td align="left">12</tgydF4y2Bad> <td align="center">90年</tgydF4y2Bad> <td align="center">44</tgydF4y2Bad> <td align="center">42</tgydF4y2Bad> <td align="center">99年</tgydF4y2Bad> <td align="center">12.109</tgydF4y2Bad> <td align="center">0.0070180</tgydF4y2Bad> <td align="center">0.0015</tgydF4y2Bad> <td align="center">−0.2546</tgydF4y2Bad> <td align="center">−0.4142</tgydF4y2Bad> <td align="center">0.3847</tgydF4y2Bad> </tr> <tr class="table-tr"> <td colspan="11"> <hr class="tbody-hr"></td> </tr> </tbody> </table></td> </tr> </tbody> </table> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">表3</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/tab3/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> 特定频率和位置权重矩阵(PWM)为275 5′党卫军。共识序列(UA<gydF4y2Bab><i>亚美大陆煤层气有限公司</我></b>∣<gydF4y2Bab><i>GUAUGUU</我></b>UAAUU)可以从那些大型网站获得PWM条目,最重要的网站<gydF4y2Bab><i>大胆斜体</我></b>。的<我>χ</gydF4y2Ba我><sup>2</gydF4y2Basup>测试执行对背景频率(每个站点<gydF4y2Basvg height="6.1624999" id="M52" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.3279,C<gydF4y2Basvg height="6.1624999" id="M53" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.1915 G<gydF4y2Basvg height="6.1624999" id="M54" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.2043,你<gydF4y2Basvg height="6.1624999" id="M55" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.2763)。五个外显子的核苷酸网站标记核苷酸作为−−5 1和12个基因内区核苷酸1到12岁。内含子的PWM时几乎相同的5′UTR被排除在外。</gydF4y2Badiv> </div> </div> <div class="floats-partial-section partial-other-wrapper" data-section="tab4"> <div class="floats-partial-content fig-content-tab4"> <table class="table-group" id="tab4"> <tbody> <tr> <td> <table class="table"> <tbody> <tr> <td class="thead-hr" colspan="11"> <hr></td> </tr> <tr class="thead"> <td align="left">网站</tgydF4y2Bad> <td align="center">一个</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> <td align="center">G</tgydF4y2Bad> <td align="center">U</tgydF4y2Bad> <td align="center"><i>χ</我><sup>2</gydF4y2Basup></td> <td align="center"><span style="width: 11.75px;"> <svg height="10.325" id="M57" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">一个</tgydF4y2Bad> <td align="center">C</tgydF4y2Bad> <td align="center">G</tgydF4y2Bad> <td align="center">U</tgydF4y2Bad> </tr> <tr> <td class="thead-hr" colspan="11"> <hr></td> </tr> <tr> <td align="left">−12</tgydF4y2Bad> <td align="center">70年</tgydF4y2Bad> <td align="center">58</tgydF4y2Bad> <td align="center">37</tgydF4y2Bad> <td align="center">136年</tgydF4y2Bad> <td align="center">51.729</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center">−0.4898</tgydF4y2Bad> <td align="center">0.0122</tgydF4y2Bad> <td align="center">−0.7264</tgydF4y2Bad> <td align="center"><b><i>0.7114</我></b></td> </tr> <tr> <td align="left">−11</tgydF4y2Bad> <td align="center">79年</tgydF4y2Bad> <td align="center">51</tgydF4y2Bad> <td align="center">23</tgydF4y2Bad> <td align="center">148年</tgydF4y2Bad> <td align="center">79.511</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center">−0.3161</tgydF4y2Bad> <td align="center">−0.1727</tgydF4y2Bad> <td align="center">−1.4074</tgydF4y2Bad> <td align="center"><b><i>0.8332</我></b></td> </tr> <tr> <td align="left">−10</tgydF4y2Bad> <td align="center">86年</tgydF4y2Bad> <td align="center">45</tgydF4y2Bad> <td align="center">14</tgydF4y2Bad> <td align="center">156年</tgydF4y2Bad> <td align="center">105.131</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center">−0.1941</tgydF4y2Bad> <td align="center">−0.3525</tgydF4y2Bad> <td align="center">−2.1155</tgydF4y2Bad> <td align="center"><b><i>0.9090</我></b></td> </tr> <tr> <td align="left">−9</tgydF4y2Bad> <td align="center">43</tgydF4y2Bad> <td align="center">33</tgydF4y2Bad> <td align="center">23</tgydF4y2Bad> <td align="center">202年</tgydF4y2Bad> <td align="center">236.063</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center">−1.1886</tgydF4y2Bad> <td align="center">−0.7978</tgydF4y2Bad> <td align="center">−1.4074</tgydF4y2Bad> <td align="center"><b><i>1.2812</我></b></td> </tr> <tr> <td align="left">−8</tgydF4y2Bad> <td align="center">56</tgydF4y2Bad> <td align="center">43</tgydF4y2Bad> <td align="center">31日</tgydF4y2Bad> <td align="center">171年</tgydF4y2Bad> <td align="center">130.216</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center">−0.8100</tgydF4y2Bad> <td align="center">−0.4178</tgydF4y2Bad> <td align="center">−0.9801</tgydF4y2Bad> <td align="center"><b><i>1.0412</我></b></td> </tr> <tr> <td align="left">−7</tgydF4y2Bad> <td align="center">102年</tgydF4y2Bad> <td align="center">35</tgydF4y2Bad> <td align="center">31日</tgydF4y2Bad> <td align="center">133年</tgydF4y2Bad> <td align="center">54.256</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center">0.0512</tgydF4y2Bad> <td align="center">−0.7134</tgydF4y2Bad> <td align="center">−0.9801</tgydF4y2Bad> <td align="center"><b><i>0.6793</我></b></td> </tr> <tr> <td align="left">−6</tgydF4y2Bad> <td align="center">103年</tgydF4y2Bad> <td align="center">46</tgydF4y2Bad> <td align="center">38</tgydF4y2Bad> <td align="center">114年</tgydF4y2Bad> <td align="center">23.130</tgydF4y2Bad> <td align="center">0.0000380</tgydF4y2Bad> <td align="center">0.0653</tgydF4y2Bad> <td align="center">−0.3210</tgydF4y2Bad> <td align="center">−0.6881</tgydF4y2Bad> <td align="center"><b><i>0.4574</我></b></td> </tr> <tr> <td align="left">−5</tgydF4y2Bad> <td align="center">One hundred.</tgydF4y2Bad> <td align="center">36</tgydF4y2Bad> <td align="center">25</tgydF4y2Bad> <td align="center">140年</tgydF4y2Bad> <td align="center">68.925</tgydF4y2Bad> <td align="center">0.0000000</tgydF4y2Bad> <td align="center">0.0228</tgydF4y2Bad> <td align="center">−0.6729</tgydF4y2Bad> <td align="center">−1.2882</tgydF4y2Bad> <td align="center"><b><i>0.7532</我></b></td> </tr> <tr> <td align="left">−4</tgydF4y2Bad> <td align="center">145年</tgydF4y2Bad> <td align="center">27</tgydF4y2Bad> <td align="center">41</tgydF4y2Bad> <td align="center">88年</tgydF4y2Bad> <td align="center">45.473</tgydF4y2Bad> <td align="center">0.0000001</tgydF4y2Bad> <td align="center"><b><i>0.5574</我></b></td> <td align="center">−1.0854</tgydF4y2Bad> <td align="center">−0.5790</tgydF4y2Bad> <td align="center">0.0850</tgydF4y2Bad> </tr> <tr> <td align="left">−3</tgydF4y2Bad> <td align="center">15</tgydF4y2Bad> <td align="center">127年</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">159年</tgydF4y2Bad> <td align="center">284.824</tgydF4y2Bad> <td align="center">0.0000002</tgydF4y2Bad> <td align="center">−2.6877</tgydF4y2Bad> <td align="center"><b><i>1.1404</我></b></td> <td align="center">−8.2350</tgydF4y2Bad> <td align="center"><b><i>0.9364</我></b></td> </tr> <tr> <td align="left">−2</tgydF4y2Bad> <td align="center">299年</tgydF4y2Bad> <td align="center">1</tgydF4y2Bad> <td align="center">1</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">605.789</tgydF4y2Bad> <td align="center">0.0000003</tgydF4y2Bad> <td align="center"><b><i>1.5998</我></b></td> <td align="center">−5.5977</tgydF4y2Bad> <td align="center">−5.6756</tgydF4y2Bad> <td align="center">−8.2346</tgydF4y2Bad> </tr> <tr> <td align="left">−1</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">301年</tgydF4y2Bad> <td align="center">0</tgydF4y2Bad> <td align="center">1171.443</tgydF4y2Bad> <td align="center">0.0000004</tgydF4y2Bad> <td align="center">−8.2345</tgydF4y2Bad> <td align="center">−8.2351</tgydF4y2Bad> <td align="center"><b><i>2.2908</我></b></td> <td align="center">−8.2346</tgydF4y2Bad> </tr> <tr> <td align="left">1</tgydF4y2Bad> <td align="center">109年</tgydF4y2Bad> <td align="center">39</tgydF4y2Bad> <td align="center">74年</tgydF4y2Bad> <td align="center">79年</tgydF4y2Bad> <td align="center">9.936</tgydF4y2Bad> <td align="center">0.0191208</tgydF4y2Bad> <td align="center">0.1467</tgydF4y2Bad> <td align="center">−0.5580</tgydF4y2Bad> <td align="center">0.2697</tgydF4y2Bad> <td align="center">−0.0701</tgydF4y2Bad> </tr> <tr> <td align="left">2</tgydF4y2Bad> <td align="center">84年</tgydF4y2Bad> <td align="center">66年</tgydF4y2Bad> <td align="center">55</tgydF4y2Bad> <td align="center">96年</tgydF4y2Bad> <td align="center">6.036</tgydF4y2Bad> <td align="center">0.1098600</tgydF4y2Bad> <td align="center">−0.2279</tgydF4y2Bad> <td align="center">0.1981</tgydF4y2Bad> <td align="center">−0.1571</tgydF4y2Bad> <td align="center">0.2102</tgydF4y2Bad> </tr> <tr> <td align="left">3</tgydF4y2Bad> <td align="center">103年</tgydF4y2Bad> <td align="center">58</tgydF4y2Bad> <td align="center">50</tgydF4y2Bad> <td align="center">90年</tgydF4y2Bad> <td align="center">2.969</tgydF4y2Bad> <td align="center">0.3964877</tgydF4y2Bad> <td align="center">0.0653</tgydF4y2Bad> <td align="center">0.0122</tgydF4y2Bad> <td align="center">−0.2940</tgydF4y2Bad> <td align="center">0.1173</tgydF4y2Bad> </tr> <tr> <td align="left">4</tgydF4y2Bad> <td align="center">96年</tgydF4y2Bad> <td align="center">45</tgydF4y2Bad> <td align="center">56</tgydF4y2Bad> <td align="center">104年</tgydF4y2Bad> <td align="center">8.655</tgydF4y2Bad> <td align="center">0.0342400</tgydF4y2Bad> <td align="center">−0.0359</tgydF4y2Bad> <td align="center">−0.3525</tgydF4y2Bad> <td align="center">−0.1312</tgydF4y2Bad> <td align="center">0.3253</tgydF4y2Bad> </tr> <tr> <td align="left">5</tgydF4y2Bad> <td align="center">One hundred.</tgydF4y2Bad> <td align="center">69年</tgydF4y2Bad> <td align="center">39</tgydF4y2Bad> <td align="center">93年</tgydF4y2Bad> <td align="center">11.698</tgydF4y2Bad> <td align="center">0.0084938</tgydF4y2Bad> <td align="center">0.0228</tgydF4y2Bad> <td align="center">0.2620</tgydF4y2Bad> <td align="center">−0.6508</tgydF4y2Bad> <td align="center">0.1645</tgydF4y2Bad> </tr> <tr class="table-tr"> <td colspan="11"> <hr class="tbody-hr"></td> </tr> </tbody> </table></td> </tr> </tbody> </table> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">表4</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/tab4/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> 特定频率和位置权重矩阵(PWM) 301 3′党卫军。共识序列(<gydF4y2Bab><i>UUUUUUUUAYAG</我></b>∣GCUUC)可以从那些大型网站获得PWM条目,最重要的网站<gydF4y2Bab><i>大胆斜体</我></b>。的<我>χ</gydF4y2Ba我><sup>2</gydF4y2Basup>测试执行每个站点在预期的背景下的频率。网站用first-exon网站标记为1。内含子的PWM时几乎相同的5′UTR被排除在外。</gydF4y2Badiv> </div> </div> <div class="floats-partial-section partial-carousel-wrapper" data-section="fig1"> <div class="floats-partial-content fig-content-fig1"> <div class="partial-carousel--single-image"> <img alt="(一)”的名字=”2121年46。fig.001a" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.001a.jpg"> <br> <strong>(一)</gydF4y2Bastrong> </div> <div class="partial-carousel--single-image"> <img alt="(b)”的名字=”2121年46。fig.001b" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.001b.jpg"> <br> <strong>(b)</gydF4y2Bastrong> </div> </div> <div class="floats-partial-middle"> <div class="floats-partial-middle--thumbs fig-thumbs-fig1"> <button class="partial-carousel-dot" aria-label="(a)"><img alt="(一)”的名字=”2121年46。fig.001a" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.001a.jpg"><br><strong>(一)</gydF4y2Bastrong></button> <button class="partial-carousel-dot" aria-label="(b)"><img alt="(b)”的名字=”2121年46。fig.001b" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.001b.jpg"><br><strong>(b)</gydF4y2Bastrong></button> </div> <div class="partial-carousel-next-prev fig-nav-fig1"> <button class="carousel-prev" aria-label="Previous"></button> <button class="carousel-next" aria-label="Next"></button> </div> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">图1</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/fig1/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> <svg height="6.1624999" id="M58" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg> <svg height="6.1624999" id="M59" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg> <svg height="6.1624999" id="M60" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg> <svg height="6.1624999" id="M61" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg> <svg height="16.1" id="M62" style="vertical-align:-3.2729pt" version="1.1" viewbox="0 0 109.0375 16.1" width="109.0375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,16.1)"> <g transform="translate(72,-59.12)"> <text transform="matrix(1,0,0,-1,-71.95,62.44)"> <tspan style="font-size: 12.50px; " x="12.73" y="0.029999999"> ∑</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.24" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="0" y="0"> −</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="8.5645552" y="0"> (</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-38.28,59.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-35.06,62.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> l</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="3.4758339" y="0"> o</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="9.727334" y="0"> g</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-12.83,59.27)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 2</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-7.96,62.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> (</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,3.63,59.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,6.85,62.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> )</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="4.1634989" y="0"> ]</tgydF4y2Baspan> </text> </g> </g> </svg> <svg height="14.3625" id="M63" style="vertical-align:-3.2316pt" version="1.1" viewbox="0 0 13.4375 14.3625" width="13.4375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,14.3625)"> <g transform="translate(72,-60.51)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-64.52,60.66)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg> <svg height="13.45" id="M64" style="vertical-align:-2.21957pt" version="1.1" viewbox="0 0 124.2 13.45" width="124.2" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,13.45)"> <g transform="translate(72,-61.24)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="5.9639311" y="0"> (</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.12743" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.167818" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="31.194984" y="0"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="40.572235" y="0"> C</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="48.911736" y="0"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="58.288986" y="0"> G</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="71.479652" y="0"> o</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="77.731148" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="86.070648" y="0"> U</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="95.097816" y="0"> )</tgydF4y2Baspan> </text> </g> </g> </svg> <table> 序列标识的5′ss (a)和3′ss (b),与背景产生频率指定为一个0。3279,C0gydF4y2Ba。1915G0gydF4y2Ba。2043,你0。2763年。的核苷酸频率低于预期策划颠倒。竖线是指数计算的信息,gydF4y2Ba在那里gydF4y2Ba核苷酸的频率gydF4y2Ba在每个站点。</t一个ble> </div> </div> </div> <p>鉴于重大PWM 5′ss和3′党卫军,我们想知道哪个单个核苷酸网站(17)的意义。所有17个核苷酸的5′ss和16个核苷酸的3′党卫军在0.05显著水平当experimentwise错误率不是控制了(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab3/" target="_blank">3</一个>和<一个href="//www.newsama.com/journals/ijg/2011/212146/tab4/" target="_blank">4</一个>)。一个流行的统计方法控制experimentwise错误率是错误发现率的方法(罗斯福)<一个href="#B54">54</一个>,<gydF4y2Ba一个href="#B55">55</一个>]。经典的罗斯福方法(<一个href="#B54">54</一个>),通常被称为Benjamini-Hochberg过程或者只是BH过程,<gydF4y2Basvg height="9.875" id="M65" style="vertical-align:-2.29482pt;width:7.7624998px;" version="1.1" viewbox="0 0 7.7624998 9.875" width="7.7624998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.875)"> <g transform="translate(72,-64.1)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>值按升序和计算<gydF4y2Basvg height="11.05" id="M66" style="vertical-align:-3.2316pt;width:63.049999px;" version="1.1" viewbox="0 0 63.049999 11.05" width="63.049999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.05)"> <g transform="translate(72,-63.16)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-65.84,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.9665918" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3996477" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.832704" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.26576" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.151648" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.037537" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-41.37,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-39.18,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.837584" y="0"> H</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-27.02,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>(下标BH代表BH过程)的<gydF4y2Basvg height="10.975" id="M67" style="vertical-align:-0.13794pt;width:17.275px;" version="1.1" viewbox="0 0 17.275 10.975" width="17.275" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.975)"> <g transform="translate(72,-63.22)"> <text transform="matrix(1,0,0,-1,-71.95,63.41)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="3.888433" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="7.4517879" y="0"> h</tgydF4y2Baspan> </text> </g> </g> </svg> <svg height="9.875" id="M68" style="vertical-align:-2.29482pt;width:7.7624998px;" version="1.1" viewbox="0 0 7.7624998 9.875" width="7.7624998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.875)"> <g transform="translate(72,-64.1)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>值作为<gydF4y2Baspan class="equation" id="EEq2"> <svg height="31.075001" id="M69" preserveaspectratio="xMaxYMin" style="vertical-align:-0pt;width:700.9375px;" version="1.1" viewbox="0 0 700.9375 31.075001" width="700.9375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,31.075)"> <g transform="translate(72,-47.14)"> <text transform="matrix(1,0,0,-1,163.32,55.95)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,169.43,52.82)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.9665918" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3996477" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.832704" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.26576" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.151648" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.037537" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,193.9,52.82)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,196.09,52.82)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.837584" y="0"> H</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,208.25,52.82)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,217.13,55.95)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="13.23" y="-8.3000002"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="21.73204" y="-8.3000002"> ⋅</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="28.083565" y="-8.3000002"> </tgydF4y2Baspan> </text> <g> <path d="m 230.36,59.07 18.75,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path> </g> <text transform="matrix(1,0,0,-1,233.85,47.19)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="16.459999" y="-8.7600002"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="240.27" y="-8.7600002"> (</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="244.4335" y="-8.7600002"> 2</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="250.685" y="-8.7600002"> )</tgydF4y2Baspan> </text> </g> </g> </svg></span>在哪里<gydF4y2Basvg height="9.875" id="M70" style="vertical-align:-2.29482pt;width:7.9124999px;" version="1.1" viewbox="0 0 7.9124999 9.875" width="7.9124999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.875)"> <g transform="translate(72,-64.1)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>罗斯福(如0.05),<gydF4y2Basvg height="9.9250002" id="M71" style="vertical-align:-0.13794pt;width:4.9875002px;" version="1.1" viewbox="0 0 4.9875002 9.9250002" width="4.9875002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.925)"> <g transform="translate(72,-64.06)"> <text transform="matrix(1,0,0,-1,-71.95,64.25)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>的排名<gydF4y2Basvg height="9.875" id="M72" style="vertical-align:-2.29482pt;width:7.7624998px;" version="1.1" viewbox="0 0 7.7624998 9.875" width="7.7624998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.875)"> <g transform="translate(72,-64.1)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>排序数组的值<gydF4y2Basvg height="9.875" id="M73" style="vertical-align:-2.29482pt;width:7.7624998px;" version="1.1" viewbox="0 0 7.7624998 9.875" width="7.7624998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.875)"> <g transform="translate(72,-64.1)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>值,<gydF4y2Basvg height="10.325" id="M74" style="vertical-align:-0.0pt;width:14.8375px;" version="1.1" viewbox="0 0 14.8375 10.325" width="14.8375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>是测试的数量(即。的数量<gydF4y2Basvg height="9.875" id="M75" style="vertical-align:-2.29482pt;width:7.7624998px;" version="1.1" viewbox="0 0 7.7624998 9.875" width="7.7624998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.875)"> <g transform="translate(72,-64.1)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>值,在本例中17日)。如果<gydF4y2Basvg height="10.7375" id="M76" style="vertical-align:-0.13794pt;width:8.6000004px;" version="1.1" viewbox="0 0 8.6000004 10.7375" width="8.6000004" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.7375)"> <g transform="translate(72,-63.41)"> <text transform="matrix(1,0,0,-1,-71.95,63.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>是最大的<gydF4y2Basvg height="9.9250002" id="M77" style="vertical-align:-0.13794pt;width:4.9875002px;" version="1.1" viewbox="0 0 4.9875002 9.9250002" width="4.9875002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.925)"> <g transform="translate(72,-64.06)"> <text transform="matrix(1,0,0,-1,-71.95,64.25)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>满足的条件<gydF4y2Basvg height="13.675" id="M78" style="vertical-align:-3.2316pt;width:94.099998px;" version="1.1" viewbox="0 0 94.099998 13.675" width="94.099998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,13.675)"> <g transform="translate(72,-61.06)"> <text transform="matrix(1,0,0,-1,-71.95,64.34)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-65.84,61.21)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-59.14,64.34)"> <tspan style="font-size: 12.50px; " x="0" y="0"> ≤</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="12.027886" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-40.99,61.21)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.95784" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3908958" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.823952" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.257008" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.142897" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.028784" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-16.52,61.21)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-14.33,61.21)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.837584" y="0"> H</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-2.18,61.21)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>,然后我们拒绝假说<gydF4y2Basvg height="14.2375" id="M79" style="vertical-align:-3.13504pt;width:19.862499px;" version="1.1" viewbox="0 0 19.862499 14.2375" width="19.862499" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,14.2375)"> <g transform="translate(72,-60.61)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-61.03,60.66)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 1</tgydF4y2Baspan> </text> </g> </g> </svg>来<gydF4y2Basvg height="14.3625" id="M80" style="vertical-align:-3.2316pt;width:20.325001px;" version="1.1" viewbox="0 0 20.325001 14.3625" width="20.325001" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,14.3625)"> <g transform="translate(72,-60.51)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-61.03,60.66)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>。在我们的例子中,所有的17个核苷酸网站统计上显著的基础上<gydF4y2Basvg height="11.05" id="M81" style="vertical-align:-3.2316pt;width:63.049999px;" version="1.1" viewbox="0 0 63.049999 11.05" width="63.049999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.05)"> <g transform="translate(72,-63.16)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-65.84,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.9665918" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3996477" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.832704" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.26576" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.151648" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.037537" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-41.37,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-39.18,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.837584" y="0"> H</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-27.02,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab5/" target="_blank">5</一个>)。</gydF4y2Bap> <div class="floats-partial-section partial-other-wrapper" data-section="tab5"> <div class="floats-partial-content fig-content-tab5"> <table class="table-group" id="tab5"> <tbody> <tr> <td> <table class="table"> <tbody> <tr> <td class="thead-hr" colspan="4"> <hr></td> </tr> <tr class="thead"> <td align="left">网站</tgydF4y2Bad> <td align="center"><span style="width: 11.75px;"> <svg height="10.325" id="M82" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">pBH<gydF4y2Basup>(1)</gydF4y2Basup></td> <td align="center">pBY<gydF4y2Basup>(2)</gydF4y2Basup></td> </tr> <tr> <td class="thead-hr" colspan="4"> <hr></td> </tr> <tr> <td align="left">1</tgydF4y2Bad> <td align="center">* 0.0000000000<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.002941</tgydF4y2Bad> <td align="center">0.000855</tgydF4y2Bad> </tr> <tr> <td align="left">5</tgydF4y2Bad> <td align="center">* 0.0000000000<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.005882</tgydF4y2Bad> <td align="center">0.001710</tgydF4y2Bad> </tr> <tr> <td align="left">2</tgydF4y2Bad> <td align="center">* 0.0000000000<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.008824</tgydF4y2Bad> <td align="center">0.002565</tgydF4y2Bad> </tr> <tr> <td align="left">6</tgydF4y2Bad> <td align="center">* 0.0000000000<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.011765</tgydF4y2Bad> <td align="center">0.003420</tgydF4y2Bad> </tr> <tr> <td align="left">3</tgydF4y2Bad> <td align="center">* 0.0000000000<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.014706</tgydF4y2Bad> <td align="center">0.004276</tgydF4y2Bad> </tr> <tr> <td align="left">4</tgydF4y2Bad> <td align="center">* 0.0000000000<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.017647</tgydF4y2Bad> <td align="center">0.005131</tgydF4y2Bad> </tr> <tr> <td align="left">7</tgydF4y2Bad> <td align="center">* 0.0000000000<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.020588</tgydF4y2Bad> <td align="center">0.005986</tgydF4y2Bad> </tr> <tr> <td align="left">−2</tgydF4y2Bad> <td align="center">* 0.0000004842<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.023529</tgydF4y2Bad> <td align="center">0.006841</tgydF4y2Bad> </tr> <tr> <td align="left">−3</tgydF4y2Bad> <td align="center">* 0.0000013734<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.026471</tgydF4y2Bad> <td align="center">0.007696</tgydF4y2Bad> </tr> <tr> <td align="left">−1</tgydF4y2Bad> <td align="center">* 0.0000030965<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.029412</tgydF4y2Bad> <td align="center">0.008551</tgydF4y2Bad> </tr> <tr> <td align="left">9</tgydF4y2Bad> <td align="center">* 0.0002619304<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.032353</tgydF4y2Bad> <td align="center">0.009406</tgydF4y2Bad> </tr> <tr> <td align="left">10</tgydF4y2Bad> <td align="center">* 0.0006307900<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.035294</tgydF4y2Bad> <td align="center">0.010261</tgydF4y2Bad> </tr> <tr> <td align="left">12</tgydF4y2Bad> <td align="center">* 0.0025004071<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.038235</tgydF4y2Bad> <td align="center">0.011116</tgydF4y2Bad> </tr> <tr> <td align="left">11</tgydF4y2Bad> <td align="center">* 0.0033589734<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.041176</tgydF4y2Bad> <td align="center">0.011971</tgydF4y2Bad> </tr> <tr> <td align="left">8</tgydF4y2Bad> <td align="center">* 0.0084455695<gydF4y2Basup>__</gydF4y2Basup></td> <td align="center">0.044118</tgydF4y2Bad> <td align="center">0.012827</tgydF4y2Bad> </tr> <tr> <td align="left">−5</tgydF4y2Bad> <td align="center">* 0.0177349476</tgydF4y2Bad> <td align="center">0.047059</tgydF4y2Bad> <td align="center">0.013682</tgydF4y2Bad> </tr> <tr> <td align="left">−4</tgydF4y2Bad> <td align="center">* 0.0182291629</tgydF4y2Bad> <td align="center">0.050000</tgydF4y2Bad> <td align="center">0.014537</tgydF4y2Bad> </tr> <tr class="table-tr"> <td colspan="4"> <hr class="tbody-hr"></td> </tr> </tbody> </table></td> </tr> <tr class="table-fn"> <td><sup>(1)</gydF4y2Basup>至关重要的<gydF4y2Basvg height="10.325" id="M83" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>基于[<一个href="#B54">54</一个>]。<gydF4y2Babr><sup>(2)</gydF4y2Basup>至关重要的<gydF4y2Basvg height="10.325" id="M84" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>基于[<一个href="#B55">55</一个>]。<gydF4y2Babr>*重要的标准(<一个href="#B54">54</一个>]。<gydF4y2Babr><sup>__</gydF4y2Basup>重要的标准(<一个href="#B55">55</一个>]。<gydF4y2Babr></td> </tr> </tbody> </table> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">表5</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/tab5/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> 评估统计学意义的单个核苷酸网站(网站,与外显子5核苷酸边贴上−−5 1和12基因内区一侧标记1 - 12)5′党卫军的两种类型的错误发现率。</gydF4y2Badiv> </div> </div> <p>上面的罗斯福过程中假设测试统计数据是独立的或积极的依赖和更为保守的罗斯福过程已经被开发出来,它放松假设[<一个href="#B55">55</一个>]。这种方法,通常称为Benjamini-Yekutieli或简单的程序,计算<gydF4y2Basvg height="11.05" id="M85" style="vertical-align:-3.2316pt;width:62.387501px;" version="1.1" viewbox="0 0 62.387501 11.05" width="62.387501" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.05)"> <g transform="translate(72,-63.16)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-65.84,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.9665918" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3996477" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.832704" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.26576" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.151648" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.037537" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-41.37,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-39.18,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.3212161" y="0"> Y</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-27.55,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>为<gydF4y2Basvg height="10.975" id="M86" style="vertical-align:-0.13794pt;width:17.275px;" version="1.1" viewbox="0 0 17.275 10.975" width="17.275" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.975)"> <g transform="translate(72,-63.22)"> <text transform="matrix(1,0,0,-1,-71.95,63.41)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="3.888433" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="7.4517879" y="0"> h</tgydF4y2Baspan> </text> </g> </g> </svg>假设为<gydF4y2Baspan class="equation" id="EEq3"> <svg height="39.512501" id="M87" preserveaspectratio="xMaxYMin" style="vertical-align:-0pt;width:700.9375px;" version="1.1" viewbox="0 0 700.9375 39.512501" width="700.9375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,39.5125)"> <g transform="translate(72,-40.39)"> <text transform="matrix(1,0,0,-1,112.11,55.95)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,118.23,52.82)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.95784" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3908958" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.823952" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.257008" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.142897" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.028784" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,142.7,52.82)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,144.89,52.82)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.3124642" y="0"> Y</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,156.52,52.82)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,165.4,55.95)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="30.58" y="-8.3000002"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="39.082039" y="-8.3000002"> ⋅</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="45.433563" y="-8.3000002"> </tgydF4y2Baspan> </text> <g> <path d="m 178.63,59.07 53.45,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path> </g> <text transform="matrix(1,0,0,-1,178.63,44.24)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="12.73" y="0.029999999"> ∑</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,202.79,50.47)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="0" y="9.9399996"> </tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.7218721" y="9.9399996"> =</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="8.7169924" y="9.9399996"> 1</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,218.46,44.24)"> <tspan style="font-size: 12.50px; " x="18.280001" y="-11.71"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="31.52" y="-20.940001"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="0" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="6.2515001" y="0"> /</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="9.727334" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,256.09,62.06)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.9665918" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3996477" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.832704" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.26576" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.151648" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.037537" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,280.56,62.06)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,282.75,62.06)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.837584" y="0"> H</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,294.91,62.06)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> <g> <path d="m 249.98,59.07 50.34,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path> </g> <text transform="matrix(1,0,0,-1,254.79,44.21)"> <tspan style="font-size: 12.50px; " x="0" y="0"> ∑</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,266.22,50.47)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="0" y="9.9399996"> </tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.7218721" y="9.9399996"> =</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="8.7169924" y="9.9399996"> 1</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,281.89,44.24)"> <tspan style="font-size: 12.50px; " x="0" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="6.2515001" y="0"> /</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="9.727334" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="19.620001" y="-11.71"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="192.23" y="-11.71"> (</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="196.39349" y="-11.71"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="202.645" y="-11.71"> )</tgydF4y2Baspan> </text> </g> </g> </svg></span>与<gydF4y2Basvg height="10.8125" id="M88" style="vertical-align:-0.10033pt;width:48.4375px;" version="1.1" viewbox="0 0 48.4375 10.8125" width="48.4375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.8125)"> <g transform="translate(72,-63.35)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="14.103384" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="26.143772" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="32.395271" y="0"> 7</tgydF4y2Baspan> </text> </g> </g> </svg>在我们的例子中,<gydF4y2Basvg height="16.1" id="M89" style="vertical-align:-3.2729pt;width:139.08749px;" version="1.1" viewbox="0 0 139.08749 16.1" width="139.08749" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,16.1)"> <g transform="translate(72,-59.12)"> <text transform="matrix(1,0,0,-1,-71.95,62.41)"> <tspan style="font-size: 12.50px; " x="0" y="0"> ∑</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="13.51" y="-0.029999999"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="19.761499" y="-0.029999999"> /</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="23.237333" y="-0.029999999"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="33.477291" y="-0.029999999"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="45.517681" y="-0.029999999"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="51.76918" y="-0.029999999"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="54.894932" y="-0.029999999"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="61.146431" y="-0.029999999"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="67.397926" y="-0.029999999"> 9</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="73.649429" y="-0.029999999"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="79.900932" y="-0.029999999"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="86.152428" y="-0.029999999"> 2</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="92.403931" y="-0.029999999"> 5</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="98.655434" y="-0.029999999"> 2</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="104.90693" y="-0.029999999"> 3</tgydF4y2Baspan> </text> </g> </g> </svg>。基于<gydF4y2Basvg height="11.05" id="M90" style="vertical-align:-3.2316pt;width:62.387501px;" version="1.1" viewbox="0 0 62.387501 11.05" width="62.387501" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.05)"> <g transform="translate(72,-63.16)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-65.84,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.9665918" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3996477" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.832704" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.26576" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.151648" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.037537" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-41.37,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-39.18,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.3212161" y="0"> Y</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-27.55,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>,在5′核苷酸网站5和−−4 ss并不显著(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab5/" target="_blank">5</一个>)。</gydF4y2Bap> <p>所有17个核苷酸的3′党卫军也在0.05显著水平标准的基础上<gydF4y2Basvg height="11.05" id="M91" style="vertical-align:-3.2316pt;width:63.049999px;" version="1.1" viewbox="0 0 63.049999 11.05" width="63.049999" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.05)"> <g transform="translate(72,-63.16)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-65.84,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.9665918" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3996477" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.832704" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.26576" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.151648" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.037537" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-41.37,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-39.18,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.837584" y="0"> H</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-27.02,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>。然而,更为保守的标准的<gydF4y2Basvg height="11.05" id="M92" style="vertical-align:-3.2316pt;width:62.387501px;" version="1.1" viewbox="0 0 62.387501 11.05" width="62.387501" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.05)"> <g transform="translate(72,-63.16)"> <text transform="matrix(1,0,0,-1,-71.95,66.44)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-65.84,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="3.8858881" y="0"> r</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="6.9665918" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="9.3996477" y="0"> t</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="11.832704" y="0"> 我</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="14.26576" y="0"> c</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="18.151648" y="0"> 一个</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="22.037537" y="0"> l</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-41.37,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-39.18,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> B</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="5.3212161" y="0"> Y</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-27.55,63.31)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 8.75px; " x="2.188" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>外显子一边,五个核苷酸网站并不重要。</gydF4y2Bap> <p>没有显著差异在5′和3′之间的党卫军pwm 5′UTR 24内含子和编码区域(<gydF4y2Basvg height="11.0375" id="M93" style="vertical-align:-0.17555pt;width:71.775002px;" version="1.1" viewbox="0 0 71.775002 11.0375" width="71.775002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.0375)"> <g transform="translate(72,-63.17)"> <text transform="matrix(1,0,0,-1,-71.95,63.4)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.182001" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="38.559254" y="0"> 6</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="44.810753" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="51.062252" y="0"> 6</tgydF4y2Baspan> </text> </g> </g> </svg>5′ss pwm和<gydF4y2Basvg height="10.9125" id="M94" style="vertical-align:-0.17555pt;width:71.775002px;" version="1.1" viewbox="0 0 71.775002 10.9125" width="71.775002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.9125)"> <g transform="translate(72,-63.27)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="29.182001" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="38.559254" y="0"> 1</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="44.810753" y="0"> 8</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="51.062252" y="0"> 2</tgydF4y2Baspan> </text> </g> </g> </svg>3′党卫军pwm)。两组汇集在其余的分析。</gydF4y2Bap> <h5 id="sec3.2">3.2。基因表达和粘接强度</h5><gydF4y2Bap>我们以前一直认为,低表达基因,平均而言,有较低的内含子剪接强度(以pwm),但在pwm方差大于高表达基因。接合强度以pwm表现出预期与基因表达的关系,后者是衡量CAI(图<一个href="//www.newsama.com/journals/ijg/2011/212146/fig2/" target="_blank">2</一个>),信使rna丰富(图<一个href="//www.newsama.com/journals/ijg/2011/212146/fig3/" target="_blank">3</一个>生产(图),或者蛋白质<一个href="//www.newsama.com/journals/ijg/2011/212146/fig4/" target="_blank">4</一个>)。此外,低表达基因变异在pwm值大于高表达基因。统计测试均值和方差的差异,我们有排名基因通过基因表达,也就是说,排名分别由CAI mRNA丰富或蛋白的生产。对于每个排名,我们指定的1/3(即最高的基因表达值。,h我ghest CAI, mRNA, or protein production, resp.) as the high-expression group and another 1/3 of the genes with the lowest expression values as the low-expression group and tested the differences in mean PWMS and the variance of PWMS between the two groups. As shown in Table<一个href="//www.newsama.com/journals/ijg/2011/212146/tab7/" target="_blank">7</一个>,这两个预测持续支持,即(1)高表达基因意味着pwm值明显大于低表达基因和(2)高表达基因在pwm方差明显小于低表达基因(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab7/" target="_blank">7</一个>)。的<gydF4y2Basvg height="9.125" id="M95" style="vertical-align:-0.11285pt;width:5.0124998px;" version="1.1" viewbox="0 0 5.0124998 9.125" width="5.0124998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.125)"> <g transform="translate(72,-64.7)"> <text transform="matrix(1,0,0,-1,-71.95,64.86)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>测试使用的假设不平等的两组之间的差异。测试两组之间的差异的方差是常规方差比率<gydF4y2Basvg height="10.325" id="M96" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>以及(<一个href="#B56">56</一个>,136- - - - - -139年页)。</gydF4y2Bap> <div class="floats-partial-section partial-carousel-wrapper" data-section="fig2"> <div class="floats-partial-content fig-content-fig2"> <div class="partial-carousel--single-image"> <img alt="(一)”的名字=”2121年46。fig.002a" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.002a.jpg"> <br> <strong>(一)</gydF4y2Bastrong> </div> <div class="partial-carousel--single-image"> <img alt="(b)”的名字=”2121年46。fig.002b" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.002b.jpg"> <br> <strong>(b)</gydF4y2Bastrong> </div> </div> <div class="floats-partial-middle"> <div class="floats-partial-middle--thumbs fig-thumbs-fig2"> <button class="partial-carousel-dot" aria-label="(a)"><img alt="(一)”的名字=”2121年46。fig.002a" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.002a.jpg"><br><strong>(一)</gydF4y2Bastrong></button> <button class="partial-carousel-dot" aria-label="(b)"><img alt="(b)”的名字=”2121年46。fig.002b" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.002b.jpg"><br><strong>(b)</gydF4y2Bastrong></button> </div> <div class="partial-carousel-next-prev fig-nav-fig2"> <button class="carousel-prev" aria-label="Previous"></button> <button class="carousel-next" aria-label="Next"></button> </div> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">图2</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/fig2/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> <table> 拼接关系强度衡量位置权重矩阵得分(pwm) 5′(a)和3′(b)拼接网站(5′ss和3′ss)和基因表达来衡量密码子适应索引。</t一个ble> </div> </div> </div> <div class="floats-partial-section partial-carousel-wrapper" data-section="fig3"> <div class="floats-partial-content fig-content-fig3"> <div class="partial-carousel--single-image"> <img alt="(一)”的名字=”2121年46。fig.003a" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.003a.jpg"> <br> <strong>(一)</gydF4y2Bastrong> </div> <div class="partial-carousel--single-image"> <img alt="(b)”的名字=”2121年46。fig.003b" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.003b.jpg"> <br> <strong>(b)</gydF4y2Bastrong> </div> </div> <div class="floats-partial-middle"> <div class="floats-partial-middle--thumbs fig-thumbs-fig3"> <button class="partial-carousel-dot" aria-label="(a)"><img alt="(一)”的名字=”2121年46。fig.003a" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.003a.jpg"><br><strong>(一)</gydF4y2Bastrong></button> <button class="partial-carousel-dot" aria-label="(b)"><img alt="(b)”的名字=”2121年46。fig.003b" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.003b.jpg"><br><strong>(b)</gydF4y2Bastrong></button> </div> <div class="partial-carousel-next-prev fig-nav-fig3"> <button class="carousel-prev" aria-label="Previous"></button> <button class="carousel-next" aria-label="Next"></button> </div> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">图3</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/fig3/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> <a href="#B28">28</一个><一个href="#B27">27</一个><t一个ble> 拼接关系强度衡量位置权重矩阵得分(pwm) 5′(a)和3′(b)拼接网站(5′ss和3′ss)和基因表达来衡量mRNA丰富(]gydF4y2Ba。信使rna富足是日志转换。类似的模式时观察到mRNA丰富从豪斯泰之等。gydF4y2Ba使用)。</t一个ble> </div> </div> </div> <div class="floats-partial-section partial-carousel-wrapper" data-section="fig4"> <div class="floats-partial-content fig-content-fig4"> <div class="partial-carousel--single-image"> <img alt="(一)”的名字=”2121年46。fig.004a" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.004a.jpg"> <br> <strong>(一)</gydF4y2Bastrong> </div> <div class="partial-carousel--single-image"> <img alt="(b)”的名字=”2121年46。fig.004b" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.004b.jpg"> <br> <strong>(b)</gydF4y2Bastrong> </div> </div> <div class="floats-partial-middle"> <div class="floats-partial-middle--thumbs fig-thumbs-fig4"> <button class="partial-carousel-dot" aria-label="(a)"><img alt="(一)”的名字=”2121年46。fig.004a" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.004a.jpg"><br><strong>(一)</gydF4y2Bastrong></button> <button class="partial-carousel-dot" aria-label="(b)"><img alt="(b)”的名字=”2121年46。fig.004b" src="https://static-01.hindawi.com/articles/ijg/volume-2011/212146/figures/212146.fig.004b.jpg"><br><strong>(b)</gydF4y2Bastrong></button> </div> <div class="partial-carousel-next-prev fig-nav-fig4"> <button class="carousel-prev" aria-label="Previous"></button> <button class="carousel-next" aria-label="Next"></button> </div> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">图4</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/fig4/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> <a href="#B30">30.</一个><一个href="#B29">29日</一个><t一个ble> 拼接关系强度衡量位置权重矩阵得分(pwm) 5′(a)和3′(b)拼接网站(5′ss和3′ss)和基因表达的蛋白质合成率(gydF4y2Ba日志转换)。类似的模式时观察到蛋白质合成率被蛋白质丰度Ghaemmaghami et al。]gydF4y2Ba。</t一个ble> </div> </div> </div> <p>点在图的集群<一个href="//www.newsama.com/journals/ijg/2011/212146/fig2/" target="_blank">2</一个>与蔡大0.8和图<一个href="//www.newsama.com/journals/ijg/2011/212146/fig3/" target="_blank">3</一个>与ln (mRNA)大于3是几乎所有的核糖体蛋白编码基因高度转录(<一个href="#B57">57</一个>),有很强的拼接(高pwm)的网站。这些基因,突变削弱的接合强度拼接网站预计将是有害的。我们的结果表明自然选择可能参与维持接合强度高的高表达基因的接头地点。</gydF4y2Bap> <h5 id="sec3.3">3.3。内含子的贫穷PWMSs党卫军Nonspliceosome拼接的机制或需要额外的连接因素</h5><gydF4y2Bap>在三个基因5′ss (<我>H一个C1HFM1,</gydF4y2Ba我>和<我>HOP2</我>)最负PWMSs(−10.3544−9.2192−8.4717,职责)。这样PWMSs暗示这些基因5′党卫军已经进化到避免spliceosomal拼接的机制,因为随机核苷酸池中的17 mer组装与酵母蛋白编码基因的核苷酸频率(a<gydF4y2Basvg height="6.1624999" id="M97" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.3279,C<gydF4y2Basvg height="6.1624999" id="M98" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.1915 G<gydF4y2Basvg height="6.1624999" id="M99" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.2043,你<gydF4y2Basvg height="6.1624999" id="M100" style="vertical-align:-0.0pt;width:10.825px;" version="1.1" viewbox="0 0 10.825 6.1624999" width="10.825" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,6.1625)"> <g transform="translate(72,-67.07)"> <text transform="matrix(1,0,0,-1,-71.95,67.12)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> </text> </g> </g> </svg>0.2763)会有预期的pwm零。</gydF4y2Bap> <p>现在知道,这些基因的剪接pre-mRNA nonspliceosomal机制或需要额外的蛋白质因子基因内区。<我>H一个C1</gydF4y2Ba我>,起着关键作用展开的蛋白质反应(UPR)绑定到UPR元素(<一个href="#B58">58</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B62">62年</一个>),是为数不多的酵母基因第一外显子比第二个更长的时间(661基点和56个基点,resp),及其处理记录(即一个非传统的机制。nonspliceosomal拼接),内含子的裂解蛋白激酶Ire1p,具有核酸内切酶活性和tRNA连接酶(<一个href="#B61">61年</一个>,<gydF4y2Ba一个href="#B63">63年</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B65">65年</一个>]。</gydF4y2Bap> <p>的<我>HFM1 / MER3</我>和<我>HOP2</我>都是meiosis-specific基因,<我>HFM1</我>编码meiosis-specific DNA解旋酶(<一个href="#B66">66年</一个>,<gydF4y2Ba一个href="#B67">67年</一个>参与交叉控制和解除霍利迪连接(<一个href="#B66">66年</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B70">70年</一个>),<gydF4y2Ba我>HOP2</我>编码一个蛋白质的同源染色体之间形成减数分裂染色体联会(<一个href="#B71">71年</一个>,<gydF4y2Ba一个href="#B72">72年</一个>]。拼接的成绩单不是本构,而是严格监管。的拼接<我>HFM1 / MER3</我>成绩单是由Mer1p和Bud13p蛋白(<一个href="#B73">73年</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B75">75年</一个>]。Unspliced<我>HOP2</我>成绩单积累在细胞减数分裂(<一个href="#B33">33</一个>]。的拼接<我>HOP2</我>成绩单在很大程度上依赖于核外来体组件Rrp6蛋白质、损失<我>RRP6</gydF4y2Ba我>大幅减少的拼接<我>HOP2</我>成绩单(<一个href="#B33">33</一个>]。</gydF4y2Bap> <p>除了上面的三个基因,该基因与最小的5′ss pwm<我>BUD25</gydF4y2Ba我>pwm等于0.4267。不绑定到酵母剪接体<我>BUD25</gydF4y2Ba我>成绩单在转录(<一个href="#B33">33</一个>]。的<我>BUD25</gydF4y2Ba我>基因也与染色体隔离和减数分裂<一个href="#B76">76年</一个>]。大多数酵母基因内区可以删除没有影响,但删除<我>BUD25</gydF4y2Ba我>基因内区导致有缺陷的生长(<一个href="#B76">76年</一个>),这表明拼接是重要的函数,它的党卫军可能受到额外的限制以外的接合强度。换句话说,党卫军<我>BUD25</gydF4y2Ba我>可能不是自由发展对接合强度高。</gydF4y2Bap> <p>3′党卫军的几个基因也有负面PWMSs。的基因内区<我>H一个C1</gydF4y2Ba我>基因,由nonspliceosome机制[拼接<一个href="#B61">61年</一个>,<gydF4y2Ba一个href="#B63">63年</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B65">65年</一个>),与最小的3′ss pwm (−5.1038)。3′党卫军的基因内区第二个最小的pwm(−3.1252)属于<我>REC102</gydF4y2Ba我>也是meiosis-specific基因染色体联会(所需<一个href="#B77">77年</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B79">79年</一个>]。内含子的剪接也利用nonspliceosome机制(<一个href="#B31">31日</一个>]。基于<我>在体外</我>实验中,拼接的<我>REC102</gydF4y2Ba我>消息由酵母剪接体既效率低下(<一个href="#B80">80年</一个>和不准确的<一个href="#B80">80年</一个>,<gydF4y2Ba一个href="#B81">81年</一个>),导致许多unspliced和错误地拼接mrna。然而,在大规模鉴定酵母总记录,发现只有一个正确拼接mRNA S03052-07_G10(文件。seq在线补充材料(<一个href="#B82">82年</一个>])。正确的氨基酸序列拼接信使rna在不同中高度保守的<我>酿酒</我>物种(<一个href="#B80">80年</一个>,<gydF4y2Ba一个href="#B83">83年</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B85">85年</一个>]。总的来说,这些结果表明,<我>在活的有机体内</我>正确的连接<我>REC102</gydF4y2Ba我>在减数分裂阶段pre-mRNA需要额外因素。这些酵母协调小组的内含子剪接nonspliceosome机制或需要额外的剪接因子很穷在招聘U1 snRPNs [<一个href="#B33">33</一个>]。结果,这种基因是强烈的监管和较低或负pwm表明使用生物信息学方法来识别这些强烈的潜在调控基因。</gydF4y2Bap> <h5 id="sec3.4">3.4。著名Poly-U上游AG)二核苷酸的3′党卫军</h5><gydF4y2Bap>有效的拼接内含子在酵母的特点是poly-U束上游的3′AG)(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab4/" target="_blank">4</一个>和图<一个href="//www.newsama.com/journals/ijg/2011/212146/fig1/" target="_blank">1</一个>)。这一趋势更强,当我们排除酵母协调小组的成绩单不绑定到剪接体(结果未显示)。这样一个poly-U束可以增加效率的3′党卫军曾被证实<我>酿酒酵母</我>(<gydF4y2Ba一个href="#B86">86年</一个>]<gydF4y2Ba我>,</gydF4y2Ba我>特别是在内含子与分支点之间的长途网站和3′ss (<一个href="#B87">87年</一个>]。最近的一项研究哺乳动物基因的内含子剪接YACs(酵母人工染色体)符合提出的重要性poly-U束上游3′党卫军<我>酿酒酵母</我>(<gydF4y2Ba一个href="#B88">88年</一个>]。</gydF4y2Bap> <p>之前编译的酵母基因内区(<一个href="#B11">11</一个>,<gydF4y2Ba一个href="#B13">13</一个>)错过了poly-U束上游3′党卫军。因此,poly-U道上游3′党卫军尚未包括的功能<我>酿酒酵母</我>基因内区分子生物学教科书(例如,<一个href="#B14">14</一个>,428页)。</gydF4y2Bap> <p>poly-U道上游的酵母3′党卫军是不同段位(U和C所占比例),往往是目前上游3′党卫军在多细胞真核生物<我>粟酒裂殖酵母。</我>在<我>酵母,</我>只有你过多和C是序列中的弱势向后(如图所示标志3′ss在图<一个href="//www.newsama.com/journals/ijg/2011/212146/fig1/" target="_blank">1 (b)</一个>)。在多细胞真核生物和<我>美国非洲酒,</我>3′的段位上游党卫军对接合强度很重要(<一个href="#B89">89年</一个>),被U2AF至关重要<gydF4y2Basup>65年</gydF4y2Basup>剪接因子(<一个href="#B90">90年</一个>]。然而,尽管U2AF<gydF4y2Basup>65年</gydF4y2Basup>是高度保守的<我>美国非洲酒</我>U2一个F多细胞真核生物<gydF4y2Basup>65年</gydF4y2Basup>MUD2p同系物的出芽酵母,是高度分化而不是生存必不可少的<一个href="#B20">20.</一个>]。这可能导致的进化起源poly-U束出芽酵母。</gydF4y2Bap> <p>poly-U的存在意味着承认3′党卫军可能涉及超过简单的扫描后的第一个AG分歧点。事实上,以往的经验显示,近端PyAG没有poly-U经常跳过如果发生更多的远端PyAG poly-U [<一个href="#B86">86年</一个>]。</gydF4y2Bap> <h5 id="sec3.5">3.5。酵母协调小组弱绑定到U1 snRNPs pwm小于那些强烈</h5><gydF4y2Bap>最近的一项研究[<一个href="#B33">33</一个>)记录50酵母协调小组的mRNA未能招聘网站U1 snRNPs检测量的转录。我们测试了这些基因的可能性可能弱ss 50之间通过比较pwm基因和其他酵母协调小组。三个50基因(YOR074C、YOR221C YLR312W-A)实际上没有内含子,不应作为酵母协调小组。此外,YOR318C可疑的基因没有<我>在活的有机体内</我>证据,这是公认的基因内区是拼接。剩下的46个基因有48个内含子,与YCL005W-A YCR097W各有两个内含子。pwm是8.8138的5′ss 48个内含子和11.1978内含子的余生。不同的是非常重要的两个示例<gydF4y2Basvg height="9.125" id="M101" style="vertical-align:-0.11285pt;width:5.0124998px;" version="1.1" viewbox="0 0 5.0124998 9.125" width="5.0124998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.125)"> <g transform="translate(72,-64.7)"> <text transform="matrix(1,0,0,-1,-71.95,64.86)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>以及(<gydF4y2Basvg height="13.0125" id="M102" style="vertical-align:-1.76814pt;width:235.9875px;" version="1.1" viewbox="0 0 235.9875 13.0125" width="235.9875" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,13.0125)"> <g transform="translate(72,-61.59)"> <text transform="matrix(1,0,0,-1,-71.95,63.4)"> <tspan style="font-size: 12.50px; " x="0" y="0"> D</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="9.0271664" y="0"> F</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="19.442165" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="31.482553" y="0"> 2</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="37.734055" y="0"> 7</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="43.985554" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="50.237053" y="0"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="59.614304" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="67.003578" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="79.043968" y="0"> −</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="87.608521" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="93.860023" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="96.985771" y="0"> 6</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="103.23727" y="0"> 3</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="109.48877" y="0"> 4</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="115.74027" y="0"> 6</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="121.99177" y="0"> ,</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="131.36902" y="0"> </tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="142.25914" y="0"> <</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="154.29953" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="160.55103" y="0"> 。</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="163.67677" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="169.92827" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="176.17978" y="0"> 0</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="182.43127" y="0"> 1</tgydF4y2Baspan> </text> </g> </g> </svg>、双尾检验表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab6/" target="_blank">6</一个>)。同样的模式是3′ss(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab6/" target="_blank">6</一个>)。因此,酵母协调小组弱束缚在转录U1 snRNPs弱5′和3′党卫军比U1 snRNPs强烈束缚。</gydF4y2Bap> <div class="floats-partial-section partial-other-wrapper" data-section="tab6"> <div class="floats-partial-content fig-content-tab6"> <table class="table-group" id="tab6"> <tbody> <tr> <td> <table class="table"> <tbody> <tr> <td class="thead-hr" colspan="5"> <hr></td> </tr> <tr> <td align="left"></td> <td align="center" colspan="2">5′党卫军</tgydF4y2Bad> <td align="center" colspan="2">3′党卫军</tgydF4y2Bad> </tr> <tr> <td align="left"></td> <td align="center">NRG</tgydF4y2Bad> <td align="center">RG</tgydF4y2Bad> <td align="center">NRG</tgydF4y2Bad> <td align="center">RG</tgydF4y2Bad> </tr> <tr> <td align="center" colspan="5"> <hr></td> </tr> <tr> <td align="left">pwm的意思</tgydF4y2Bad> <td align="center">8.8138</tgydF4y2Bad> <td align="center">11.1978</tgydF4y2Bad> <td align="center">5.3129</tgydF4y2Bad> <td align="center">7.1762</tgydF4y2Bad> </tr> <tr> <td align="left">pwm Var。</tgydF4y2Bad> <td align="center">31.5069</tgydF4y2Bad> <td align="center">4.8646</tgydF4y2Bad> <td align="center">13.3017</tgydF4y2Bad> <td align="center">8.2077</tgydF4y2Bad> </tr> <tr> <td align="left"><span style="width: 14.8375px;"> <svg height="10.325" id="M104" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 14.8375 10.325" width="14.8375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">44</tgydF4y2Bad> <td align="center">231年</tgydF4y2Bad> <td align="center">49</tgydF4y2Bad> <td align="center">252年</tgydF4y2Bad> </tr> <tr> <td align="left"><span style="width: 5.0124998px;"> <svg height="9.125" id="M105" style="vertical-align:-0.11285pt" version="1.1" viewbox="0 0 5.0124998 9.125" width="5.0124998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.125)"> <g transform="translate(72,-64.7)"> <text transform="matrix(1,0,0,-1,-71.95,64.86)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">−4.6346</tgydF4y2Bad> <td align="center"></td> <td align="center">−3.9257</tgydF4y2Bad> <td align="center"></td> </tr> <tr> <td align="left"><span style="width: 11.75px;"> <svg height="10.325" id="M106" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">0.0000</tgydF4y2Bad> <td align="center"></td> <td align="center">0.0001</tgydF4y2Bad> <td align="center"></td> </tr> <tr class="table-tr"> <td colspan="5"> <hr class="tbody-hr"></td> </tr> </tbody> </table></td> </tr> </tbody> </table> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">表6</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/tab6/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> 位置权重矩阵得分(PWMSs代理连接强度)是小得多的拼接网站从intron-containing基因(协调小组)的成绩单没有招募U1 snRNPs (NRG nonrecruiting集团)比那些从协调小组的成绩单绑定U1 snRNPs招聘组(RG)。模式是一致的5′ss和3′党卫军,基于两个示例<gydF4y2Basvg height="9.125" id="M103" style="vertical-align:-0.11285pt;width:5.0124998px;" version="1.1" viewbox="0 0 5.0124998 9.125" width="5.0124998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.125)"> <g transform="translate(72,-64.7)"> <text transform="matrix(1,0,0,-1,-71.95,64.86)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>测试假设方差相等。Mann-Whitney测试产生同样的结论。</gydF4y2Badiv> </div> </div> <div class="floats-partial-section partial-other-wrapper" data-section="tab7"> <div class="floats-partial-content fig-content-tab7"> <table class="table-group" id="tab7"> <tbody> <tr> <td> <table class="table"> <tbody> <tr> <td class="thead-hr" colspan="7"> <hr></td> </tr> <tr> <td align="left"></td> <td align="center" colspan="3">5′党卫军</tgydF4y2Bad> <td align="center" colspan="3">3′党卫军</tgydF4y2Bad> </tr> <tr> <td align="left"></td> <td align="center">蔡</tgydF4y2Bad> <td align="center">lnMRNA<gydF4y2Basup>(1)</gydF4y2Basup></td> <td align="center">lnPROT<gydF4y2Basup>(2)</gydF4y2Basup></td> <td align="center">蔡</tgydF4y2Bad> <td align="center">lnMRNA</tgydF4y2Bad> <td align="center">lnPROT</tgydF4y2Bad> </tr> <tr> <td align="center" colspan="7"> <hr></td> </tr> <tr> <td align="left">N<gydF4y2Basup>(3)</gydF4y2Basup></td> <td align="center">91年</tgydF4y2Bad> <td align="center">48</tgydF4y2Bad> <td align="center">55</tgydF4y2Bad> <td align="center">One hundred.</tgydF4y2Bad> <td align="center">53</tgydF4y2Bad> <td align="center">67年</tgydF4y2Bad> </tr> <tr> <td align="left">MeanH<gydF4y2Basup>(4)</gydF4y2Basup></td> <td align="center">11.4927</tgydF4y2Bad> <td align="center">11.3128</tgydF4y2Bad> <td align="center">11.4879</tgydF4y2Bad> <td align="center">8.7188</tgydF4y2Bad> <td align="center">8.5447</tgydF4y2Bad> <td align="center">8.6004</tgydF4y2Bad> </tr> <tr> <td align="left">MeanL<gydF4y2Basup>(5)</gydF4y2Basup></td> <td align="center">9.4135</tgydF4y2Bad> <td align="center">9.7143</tgydF4y2Bad> <td align="center">9.7581</tgydF4y2Bad> <td align="center">5.1109</tgydF4y2Bad> <td align="center">4.9729</tgydF4y2Bad> <td align="center">5.3359</tgydF4y2Bad> </tr> <tr> <td align="left">DF<gydF4y2Basup>(6)</gydF4y2Basup></td> <td align="center">113年</tgydF4y2Bad> <td align="center">59</tgydF4y2Bad> <td align="center">55</tgydF4y2Bad> <td align="center">155年</tgydF4y2Bad> <td align="center">83年</tgydF4y2Bad> <td align="center">82年</tgydF4y2Bad> </tr> <tr> <td align="left"><span style="width: 11.225px;"> <svg height="10.325" id="M107" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.225 10.325" width="11.225" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">4.1635</tgydF4y2Bad> <td align="center">2.2501</tgydF4y2Bad> <td align="center">2.2411</tgydF4y2Bad> <td align="center">9.7833</tgydF4y2Bad> <td align="center">6.9719</tgydF4y2Bad> <td align="center">5.7687</tgydF4y2Bad> </tr> <tr> <td align="left"><span style="width: 11.75px;"> <svg height="10.325" id="M108" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">0.0001</tgydF4y2Bad> <td align="center">0.0282</tgydF4y2Bad> <td align="center">0.0291</tgydF4y2Bad> <td align="center">< 0.0001</tgydF4y2Bad> <td align="center">< 0.0001</tgydF4y2Bad> <td align="center">< 0.0001</tgydF4y2Bad> </tr> <tr> <td align="center" colspan="7"> <hr></td> </tr> <tr> <td align="left">VarH<gydF4y2Basup>(7)</gydF4y2Basup></td> <td align="center">3.2053</tgydF4y2Bad> <td align="center">2.8657</tgydF4y2Bad> <td align="center">2.7345</tgydF4y2Bad> <td align="center">2.6326</tgydF4y2Bad> <td align="center">3.4395</tgydF4y2Bad> <td align="center">3.4220</tgydF4y2Bad> </tr> <tr> <td align="left">VarL<gydF4y2Basup>(8)</gydF4y2Basup></td> <td align="center">10.3934</tgydF4y2Bad> <td align="center">21.3602</tgydF4y2Bad> <td align="center">24.6692</tgydF4y2Bad> <td align="center">20.0609</tgydF4y2Bad> <td align="center">10.4712</tgydF4y2Bad> <td align="center">9.9223</tgydF4y2Bad> </tr> <tr> <td align="left"><span style="width: 12.05px;"> <svg height="10.325" id="M109" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">3.2429</tgydF4y2Bad> <td align="center">7.4537</tgydF4y2Bad> <td align="center">9.0214</tgydF4y2Bad> <td align="center">7.6211</tgydF4y2Bad> <td align="center">3.0444</tgydF4y2Bad> <td align="center">2.9000</tgydF4y2Bad> </tr> <tr> <td align="left"><span style="width: 11.75px;"> <svg height="10.325" id="M110" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,10.325)"> <g transform="translate(72,-63.74)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg></span></td> <td align="center">< 0.0001</tgydF4y2Bad> <td align="center">< 0.0001</tgydF4y2Bad> <td align="center">< 0.0001</tgydF4y2Bad> <td align="center">< 0.0001</tgydF4y2Bad> <td align="center">< 0.0001</tgydF4y2Bad> <td align="center">0.0001</tgydF4y2Bad> </tr> <tr class="table-tr"> <td colspan="7"> <hr class="tbody-hr"></td> </tr> </tbody> </table></td> </tr> <tr class="table-fn"> <td><sup>(1)</gydF4y2Basup>自然对数的信使rna(丰度<一个href="#B28">28</一个>]。<gydF4y2Babr><sup>(2)</gydF4y2Basup>自然对数的蛋白质合成率(<一个href="#B30">30.</一个>]。<gydF4y2Babr><sup>(3)</gydF4y2Basup>高度的ss表达和低表达组(注意<gydF4y2Basvg height="14.2375" id="M111" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 92.400002 14.2375" width="92.400002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,14.2375)"> <g transform="translate(72,-60.61)"> <text transform="matrix(1,0,0,-1,-71.95,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-61.31,60.66)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 1</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-52.96,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="12.027886" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-30.28,60.66)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 2</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-21.94,63.79)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="12.027886" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>)。<gydF4y2Babr><sup>(4)</gydF4y2Basup>意思是pwm高表达组。<gydF4y2Babr><sup>(5)</gydF4y2Basup>意味着pwm低表达组。<gydF4y2Babr><sup>(6)</gydF4y2Basup>的<gydF4y2Basvg height="9.125" id="M112" style="vertical-align:-0.11285pt" version="1.1" viewbox="0 0 5.0124998 9.125" width="5.0124998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.125)"> <g transform="translate(72,-64.7)"> <text transform="matrix(1,0,0,-1,-71.95,64.86)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> </g> </g> </svg>以及假设方差不平等。SoDF不等于(<gydF4y2Basvg height="14.6" id="M113" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 82.025002 14.6" width="82.025002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,14.6)"> <g transform="translate(72,-60.32)"> <text transform="matrix(1,0,0,-1,-71.95,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-61.31,60.37)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 1</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-53.66,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> +</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="11.340221" y="0"> </tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-31.67,60.37)"> <tspan style="font-size: 8.75px; " x="0" y="0"> 2</tgydF4y2Baspan> </text> <text transform="matrix(1,0,0,-1,-24.02,63.5)"> <tspan style="font-size: 12.50px; " x="0" y="0"> −</tgydF4y2Baspan> <tspan style="font-size: 12.50px; " x="11.340221" y="0"> 2</tgydF4y2Baspan> </text> </g> </g> </svg>)。<gydF4y2Babr><sup>(7)</gydF4y2Basup>高表达组的方差。<gydF4y2Babr><sup>(8)</gydF4y2Basup>低表达组的方差。<gydF4y2Babr></td> </tr> </tbody> </table> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">表7</gydF4y2Baspan> <a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/ijg/2011/212146/tab7/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> 测试预测,内含子在高度表达基因有更高的脉宽调制的pwm和较小的方差比低表达的基因,基因表达以CAI,信使rna和蛋白质丰度。内含子拼接通过nonspliceosomal机制被排除在外。Mann-Whitney测试产生相似的结果。所有的测试是两个跟踪。结果是几乎相同的,当信使rna丰富从微阵列<一个href="#B27">27</一个>从GATC-PCR]是用来代替,<一个href="#B28">28</一个>)或者当蛋白质丰度(<一个href="#B29">29日</一个>)是用来代替蛋白质合成率(<一个href="#B30">30.</一个>]。</gydF4y2Badiv> </div> </div> <p>目前尚不清楚为什么酵母协调小组弱束缚在转录U1 snRNPs应该弱3′ss因为3′党卫军预计不会参与招聘U1 snRNPs在转录。一个可能的解释是,一个软弱的5′党卫军不招募U1有效往往被关联到一个弱3′党卫军。</gydF4y2Bap> <h4 id="discussion">4所示。讨论</h4><gydF4y2Bap>没有大规模的实验表征的拼接效率,因此很难与pwm作为代理的接合强度拼接效率。然而,这三种酵母协调小组的拼接取决于Mer1p实验测量拼接效率表示为野生型(成绩单拼接的百分比<一个href="#B73">73年</一个>]。当<我>Mer1</gydF4y2Ba我>不是表示,这些百分比分别为32%和31%<我>一个MA1</我>、14%和13%<我>REC107 / Mer2</我>、3%和4%<我>HFM1 / Mer3</我>。相应的值时<我>Mer1</gydF4y2Ba我>71年%和72%是表示<我>一个MA1</我>、53%和59%<我>REC107 / Mer2</我>、42%和42%<我>HFM1 / Mer3</我>。符合这种排名的拼接效率<我>一个MA1</我>><gydF4y2Ba我>REC107 / Mer2</我>><gydF4y2Ba我>HFM1 / Mer3,</我>5′ss和3′党卫军pwm值是8.7838和11.4573<我>一个MA1,</我>4。6995年和5.6668<我>REC107 / Mer2</我>,−7。3825和1.3488<我>HFM1 / Mer3。</我>因此,在这个有限的情况下,实验测量了拼接效率与pwm显示了良好的一致性。</gydF4y2Bap> <p>三个额外的但间接证据表明,脉宽调制是一种合适的代理拼接的力量。首先,pwm 5′ss和3′ss与基因表达呈正相关。第二,内含子拼接nonspliceosomal机制或需要额外的蛋白质剪接因素一般有低pwm。第三,酵母协调小组,招募拼接pwm因素不佳往往低于那些绑定连接因素。这些结果说明利用pwm作为筛查工具的潜力协调小组不拼接的剪接体机制或需要额外的监管因素剪接其他真菌物种。</gydF4y2Bap> <p>特征5′ss (UA<gydF4y2Bab><i>亚美大陆煤层气有限公司</我></b>∣<gydF4y2Bab><i>GUAUGUU</我></b>UAAUU,重要网站粗斜体)和3′ss (<gydF4y2Bab><i>UUUUUUUUAYAG</我></b>∣GCUUC)酵母扩大传统的酵母共识接头地点。虽然3′党卫军的+ 1网站网站不是experimentwise错误率显著调整后,最近的实验研究表明,它确实影响拼接效率。过度使用核苷酸在这个站点是G,紧随其后的是一个,但C强烈避免(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab4/" target="_blank">4</一个>)。改变+ 1 + 1 c<我>LSM7</gydF4y2Ba我>信使rna导致拼接在下游AG∣网站(<一个href="#B81">81年</一个>]。在<我>REC102</gydF4y2Ba我>基因,在正常的3′剪接ss站点UGAAG∣网站时减少+ 1 C的改变,尤其是当核苷酸在上游AG∣C站点更改为(<一个href="#B81">81年</一个>]。我们的结果(表<一个href="//www.newsama.com/journals/ijg/2011/212146/tab4/" target="_blank">4</一个>)偏好的+ 1 r和避免−1 c证实了这些实验研究和建议的偏好+ 1 r和避免−1 c可能是酵母剪接体的拼接的一般特性。</gydF4y2Bap> <p>选择增加粘接强度预计将比在低强高转录基因转录的基因。符合期望,对高转录协调小组接合强度高于低转录协调小组和较高的协调小组mrna有效翻译比那些mrna不是有效的翻译。它早就知道,高度表示酵母基因表现出高度的codon-anticodon适应(<一个href="#B91">91年</一个>]。我们的结果表明,自然选择也在拼接操作机械。</gydF4y2Bap> <p>存在poly-U立即前3 3′′AG) ss,而不是一个段位在其他真核生物,可能出现的进化过程。polypyrimidine认可,和绑定到,U2AF<gydF4y2Basup>65年</gydF4y2Basup>在其他真核生物包括<我>美国筛</我>。的U2AF<gydF4y2Basup>65年</gydF4y2Basup>MUD2p同系物的出芽酵母,是高度分化而不是生存必不可少的<一个href="#B20">20.</一个>]。这可能导致削弱段位的进化选择限制在出芽酵母。因为出芽酵母基因组在富有,核苷酸C可能逐步取代了核苷酸T,导致过渡的段位poly-U道。然而,这mutationist假说不能解释为什么保利(U)可以提高连接效率。很可能是两种突变和选择参与保利(U)的进化前的酵母基因内区3′AG)。</gydF4y2Bap> <p>我们应该提到酵母内含子的剪接效率不仅取决于5′ss和3′党卫军,而且在分歧点序列(个基点,<一个href="#B92">92年</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B94">94年</一个>])以及间距基点和3′ss (<一个href="#B95">95年</一个>- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -- - - - - -<gydF4y2Ba一个href="#B97">97年</一个>]。例如,5′和3′党卫军<我>Yr一个1</gydF4y2Ba我>强大的pwm高,但内含子剪接调控,可能通过其非常规的分歧点网站GACUAAC(与共识UACUAAC)。</gydF4y2Bap> <h4 id="acknowledgments">确认</h4><gydF4y2Bap>本研究支持NSERC的发现和战略资助。作者感谢s Aris-Brosou g . Palidwor m . Ragonnet e .罗查a . van Weringh h .熊问:杨,x姚明讨论和评论。</gydF4y2Bap> </div> <div class="xml-content"> <h4 class="references" id="references">引用</h4><ol><l我我d="B1">p . a .锋利,“分裂基因的发现,RNA拼接。”<我>生化科学趋势</我>,30卷,不。6,279 - 281年,2005页。<gydF4y2Baspan class="reflinks">视图:<一个href="https://doi.org/10.1016/j.tibs.2005.04.002">出版商的网站</一个><span class="sep">|</gydF4y2Baspan><a href="https://scholar.google.com/scholar_lookup?title=The%20discovery%20of%20split%20genes%20and%20RNA%20splicing&author=P. A. Sharp&publication_year=2005" target="_blank">谷歌学术搜索</一个></span></li> <li id="B2">r·a·帕吉特p•j•格拉博夫斯基,m . m . Konarska”信使RNA前体的拼接,”<我>年度回顾生物化学</我>,55卷,第1150 - 1119页,1986年。<gydF4y2Baspan class="reflinks">视图:<一个href="https://scholar.google.com/scholar_lookup?title=Splicing%20of%20messenger%20RNA%20precursors&author=R. A. Padgett&author=P. J. Grabowski&author=&author=M. M. Konarska&publication_year=1986" target="_blank">谷歌学术搜索</一个></span></li> <li id="B3">h·杜和m . 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Wise&publication_year=1997" target="_blank">谷歌学术搜索</一个></span></li> <li id="B90">诉曾经和r·辛格”条件的角色U2AF内含子的剪接与非常规polypyrimidine大片,“<我>分子和细胞生物学</我>,27卷,不。20日,第7344 - 7334页,2007年。<gydF4y2Baspan class="reflinks">视图:<一个href="https://doi.org/10.1128/MCB.00627-07">出版商的网站</一个><span class="sep">|</gydF4y2Baspan><a href="https://scholar.google.com/scholar_lookup?title=A%20conditional%20role%20of%20U2AF%20in%20splicing%20of%20introns%20with%20unconventional%20polypyrimidine%20tracts&author=V. Sridharan &author=R. Singh&publication_year=2007" target="_blank">谷歌学术搜索</一个></span></li> <li id="B91">x夏”,如何转化机械的优化<我>大肠杆菌</我>,<gydF4y2Ba我>鼠伤寒沙门氏菌</我>和<我>酿酒酵母</我>?”<gydF4y2Ba我>遗传学</我>,卷149,不。1,37-44,1998页。<gydF4y2Baspan class="reflinks">视图:<一个href="https://scholar.google.com/scholar_lookup?title=How%20optimized%20is%20the%20translational%20machinery%20in%20Escherichia%20coli,%20Salmonella%20typhimurium%20and%20Saccharomyces%20cerevisiae?&author=X. Xia&publication_year=1998" target="_blank">谷歌学术搜索</一个></span></li> <li id="B92">c·g·辛普森·g·克拉克·d·戴维森,p·史密斯和j·w·s·布朗,“假定的分歧点共识序列的突变在植物基因内区减少拼接效率,”<我>植物杂志</我>,9卷,不。3、369 - 380年,1996页。<gydF4y2Baspan class="reflinks">视图:<一个href="https://scholar.google.com/scholar_lookup?title=Mutation%20of%20putative%20branchpoint%20consensus%20sequences%20in%20plant%20introns%20reduces%20splicing%20efficiency&author=C. G. Simpson&author=G. Clark&author=D. Davidson&author=P. Smith&author=&author=J. W. S. Brown&publication_year=1996" target="_blank">谷歌学术搜索</一个></span></li> <li id="B93">m·d·奇亚拉o . r . Gozani m·班尼特·Champion-Arnaud l . Palandjian和r·里德“蛋白质与外显子序列的识别,拼接网站,和分歧点剪接体装配的顺序在每个阶段,“<我>分子和细胞生物学</我>,16卷,不。7,3317 - 3326年,1996页。<gydF4y2Baspan class="reflinks">视图:<一个href="https://scholar.google.com/scholar_lookup?title=Identification%20of%20proteins%20that%20interact%20with%20exon%20sequences,%20splice%20sites,%20and%20the%20branchpoint%20sequence%20during%20each%20stage%20of%20spliceosome%20assembly&author=M. D. Chiara&author=O. R. Gozani&author=M. Bennett&author=P. Champion-Arnaud&author=L. Palandjian&author=&author=R. Reed&publication_year=1996" target="_blank">谷歌学术搜索</一个></span></li> <li id="B94">r·里德和t .则称“哺乳动物在pre-mRNA分歧点序列拼接的角色,”<我>基因和发展</我>,卷2,不。10日,1268 - 1276年,1988页。<gydF4y2Baspan class="reflinks">视图:<一个href="https://scholar.google.com/scholar_lookup?title=The%20role%20of%20the%20mammalian%20branchpoint%20sequence%20in%20pre-mRNA%20splicing&author=R. Reed &author=T. Maniatis&publication_year=1988" target="_blank">谷歌学术搜索</一个></span></li> <li id="B95">a . Cellini e .镶嵌地块和j·j·罗西,“酵母pre-messenger RNA拼接效率取决于临界分叉点和3之间的间距要求的接头地点,”<我>EMBO杂志</我>,5卷,不。5,1023 - 1030年,1986页。<gydF4y2Baspan class="reflinks">视图:<一个href="https://scholar.google.com/scholar_lookup?title=Yeast%20pre-messenger%20RNA%20splicing%20efficiency%20depends%20on%20critical%20spacing%20requirements%20between%20the%20branch%20point%20and%203'%20splice%20site&author=A. Cellini&author=E. Felder&author=&author=J. J. Rossi&publication_year=1986" target="_blank">谷歌学术搜索</一个></span></li> <li id="B96">a . Brys和b .施沃”,要求在酵母SLU7 pre-mRNA拼接是由分歧点之间的距离和3 '拼接网站”,<我>核糖核酸</我>,卷2,不。7,707 - 717年,1996页。<gydF4y2Baspan class="reflinks">视图:<一个href="https://scholar.google.com/scholar_lookup?title=Requirement%20for%20SLU7%20in%20yeast%20pre-mRNA%20splicing%20is%20dictated%20by%20the%20distance%20between%20the%20branchpoint%20and%20the%203'%20splice%20site&author=A. Brys &author=B. Schwer&publication_year=1996" target="_blank">谷歌学术搜索</一个></span></li> <li id="B97">b .通用Luukkonen和b . Seraphin branchpoint-3的作用的基因内区之间的间距和交互终端剪切位点核苷酸在3 '拼接选址<我>酿酒酵母</我>”,<我>EMBO杂志</我>,16卷,不。4、779 - 792年,1997页。<gydF4y2Baspan class="reflinks">视图:<一个href="https://doi.org/10.1093/emboj/16.4.779">出版商的网站</一个><span class="sep">|</gydF4y2Baspan><a href="https://scholar.google.com/scholar_lookup?title=The%20role%20of%20branchpoint-3'%20splice%20site%20spacing%20and%20interaction%20between%20intron%20terminal%20nucleotides%20in%203'%20splice%20site%20selection%20in%20Saccharomyces%20cerevisiae&author=B. G. M. Luukkonen &author=B. Séraphin&publication_year=1997" target="_blank">谷歌学术搜索</一个></span></li> </ol> </div> <div class="xml-content"> <h4 class="copyright" id="copyright">版权</h4><gydF4y2Bap>版权©2011 Pinchao Ma和Xuhua夏。这是一个开放的分布式下文章<一个rel="license" href="http://creativecommons.org/licenses/by/3.0/">知识共享归属许可</一个>,它允许无限制的使用、分配和复制在任何媒介,提供最初的工作是正确引用。</gydF4y2Bap> </div> </div> </article> </div> </div> </div> <div class="biblio__Wrapper-sc-1nou1lz-0 eyHfWh journal_article__BiblioIntegratorWidget-sc-12fk6zy-5 dbAANp article_widget biblioWrapper hide"> <hr> <h4 class="biblio__H4-sc-1nou1lz-2 becpDt hrTitleRef">更多相关文章</h4><gydF4y2Badiv id="biblio_widget" class="biblio__BiblioWrapper-sc-1nou1lz-1 hFGNjP"></div> </div> </div> </div> <div class="threeColumn__RightWrapper-sc-1bf4078-2 jzTMLK rightWrapper" id="rightSectionMenu"> <div> <div class="journal_article__ArticleStickyTopToolbar-sc-12fk6zy-0 gbrQxi article_sticky_top_toolbar"> <div class="sc-cBdUnI hRnXob article_top_toolbar"> <a class="sc-htpNat bUhGXt link 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height="24"><span class="sc-dRFtgE jrsDZP">下载其他格式</gydF4y2Baspan><span class="sc-gkFcWv gWTCXQ">更多的</gydF4y2Baspan></span> <span class="sc-htoDjs bMdQcY sc-gbOuXE jGPvPL icon-colored _1CwL9R82TPl2HxDleeYGDR icon-themeable" title="" font-size="11"></span> </div> <div class="sc-fnwBNb etzuBr"> <a class="sc-htpNat bUhGXt link sc-iNhVCk gWgCNb open_panel" href="//www.newsama.com/downloads/journals/ijg/2011/212146.epub" aria-label="ePub" property="background-color" color="#D8D8D8"> <div class="sc-eAKXzc WHkfb"> ePub</gydF4y2Badiv><img alt="" class="sc-EHOje jOLhQl sc-bfYoXt cGuwDn" title="" role="presentation" 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src="data:image/svg+xml;base64,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" 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