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<div class="ArticleMiddleSection_articleMiddleSection__DtVY3"> <div class="articleHeader"> <strong>原始文章|<gydF4y2Ba!-- -->开放获取</年代trong><d我vclass="articleHeader__meta"> <span>体积<gydF4y2Ba!-- -->2011年<gydF4y2Ba!-- -->|</年代pan> <span>文章的ID<gydF4y2Ba!-- -->408273年<gydF4y2Ba!-- -->|</年代pan> <span class="articleHeader__meta_doiLink"><a href="https://doi.org/10.1093/ecam/nep074" aria-label="Doi-link" target="_blank" rel="noreferrer">https://doi.org/10.1093/ecam/nep074</一个></年代pan> </div> <div class="simpleShowMore"> <button class="simpleShowMore__button">显示引用</gydF4y2Babutton> </div> <h1 class="articleHeader__title">人参(<我>人参quinquefolius</我>)、甘草(<我>乌拉尔甘草</我>)根提取物组合增加肝癌细胞(Hep-G2)的可行性</gydF4y2Bah1> <div class="articleHeader__authors"> <span class="articleHeader__authors_author"><strong>大卫·g·波波维奇</年代trong><一个href="//www.newsama.com/cdn-cgi/l/email-protection" aria-label="Mail Option"><span role="img" class="anticon"> <svg version="1.0" xmlns="http://www.w3.org/2000/svg" width="1em" height="1em" viewbox="0 0 1401 1101"> <path fill="#6ba439" d="M132.5 2.1C101.4 6 71.1 20.5 48.3 42.4c-22.7 21.7-37.5 48.3-44.6 80l-2.2 10.1v837l2.2 10.1c6.7 30 19.3 53.9 39.8 75.3 23.3 24.3 56.4 40.9 90 45 12.8 1.6 1122.2 1.6 1135 0 38.9-4.8 75.9-25.6 100-56.4 15-19.3 24.2-39 29.8-63.9l2.2-10.1v-837l-2.2-10.1c-7.1-31.8-21.9-58.3-44.6-80.1-20.2-19.2-43.9-31.7-73.6-38.5l-9.6-2.3L705 1.4c-321.9-.1-568.5.2-572.5.7zm1123.4 99.5c1.7.4 3.1 1 3.1 1.4 0 .5-125.6 87.7-279 194L700.9 490.2 422 297C268.5 190.7 143 103.4 143 103c0-.5 1-1.1 2.3-1.3 3.5-.7 1107.1-.8 1110.6-.1zM401 404c164.4 113.8 299.4 207 300 207 .6 0 135.6-93.2 300.1-207 164.5-113.9 299.3-207 299.5-207 .2 0 .4 170.9.4 379.7 0 417.9.5 386-6.1 398.3-6.2 11.6-19 21.4-31.9 24.4-5.8 1.4-67.4 1.6-562 1.6s-556.2-.2-562-1.6c-12.9-3-25.7-12.8-31.9-24.4-6.6-12.3-6.1 19.6-6.1-398.3 0-208.8.2-379.7.5-379.7S236.6 290.1 401 404z"></path> </svg></span></a>,<年代up>1</年代up></span> <span class="articleHeader__authors_author">施Yun唷<gydF4y2Ba!-- -->,<年代up>1</年代up></span> <span class="articleHeader__authors_author">和<gydF4y2Ba!-- -->(音译)<年代up>1</年代up></span> </div> <div class="simpleShowMore"> <button class="simpleShowMore__button">显示更多</gydF4y2Babutton> </div> <div class="articleHeader__timeline"> <div class="articleHeader__timeline_item "> <strong>收到了</年代trong><年代pan>2008年12月29日</年代pan> </div> <div class="articleHeader__timeline_item "> <strong>接受</年代trong><年代pan>2009年5月26日</年代pan> </div> <div class="articleHeader__timeline_item articleHeader__timeline_item_sticky"> <strong>发表</年代trong><年代pan>2011年5月02</年代pan> </div> </div> </div> <div class="articleBody"> <div class="xml-content"> <h4 class="header" id="abstract">文摘</gydF4y2Bah4> <p>西洋参的结合增强效应(<我>人参quinquefolius</我>)、甘草(<我>乌拉尔甘草</我>)根提取物对肝癌细胞系(Hep-G2)。isobolographic分析是用来表达协同的可能性,添加剂或对立的两者之间的交互提取。人参和甘草的根都是广泛应用于中药制剂来治疗各种疾病。然而,草药的效果在培养Hep-G2细胞结合目前未知。人参(GE)和甘草(LE)提取物均能降低细胞生存能力。LC50值,72 h后,被发现是0.64±0.02毫克/毫升(GE)和0.53±0.02毫克/毫升(LE)。isobologram是策划,其中包括五个理论lc50计算,基于固定的分数组合人参甘草提取物的方法建立的可加性。所有组合的通用电气勒(1/5,1/3、1/2 2/3,4/5)产生影响Hep-G2细胞生存能力,但他们都发现是敌对的。分数的LC50 1/3、1/2, 2/3是23%、21%和18%以上理论毒杀。乳酸脱氢酶释放表示,通用电气的比例增加超过50%,影响膜透性增加。 Cell-cycle analysis showed a slight but significant arrest at the G1 phase of cell cycle for LE. Both GE and LE reduced Hep-G2 viability independently; however, the combinations of both extracts were found to have an antagonistic effect on cell viability and increased cultured Hep-G2 survival.</p> <span class="end-abs"></span> <h4 id="introduction">1。介绍</gydF4y2Bah4> <p>西洋参(<我>人参quinquefolius</我>)和亚洲甘草(<我>乌拉尔甘草</我>)根历史悠久中国传统医学(中医)的使用。这些根通常与其他草药成分结合使用的目的,提高中药制剂的有效性。人参、甘草根可能是最利用中药成分(<一个href="#B1">1</一个>),现在两根也利用营养食品和功能性食品行业的所谓的生物活性和功能性质<一个href="#B2">2</一个>- - - - - -<一个href="#B4">4</一个>]。</gydF4y2Bap> <p>人参中的主要生物活性成分被认为是一群达玛烷三萜皂苷、人参皂苷,也被称为(<一个href="#B5">5</一个>),一个主要的甘草中有效成分甘草酸,它是一个齐墩果烷三萜烯皂素。两根还含有多种其他植物化学的物质如多糖和多酚(<一个href="#B4">4</一个>,<gydF4y2Ba一个href="#B6">6</一个>),或许可以添加额外的好处酊剂或煎煮。</gydF4y2Bap> <p>甘草根提取物和甘草酸已报告有细胞毒性和hepato-protective属性。据报道,从甘草中提取甘草酸防止黄曲霉毒素B<年代ub>1</年代ub>Hep-G2细胞损伤(<一个href="#B7">7</一个>)和自由脂肪酸减少段肝lipotoxicity Hep-G2细胞培养和high-fat-diet-induced lipotoxicity在老鼠<一个href="#B8">8</一个>]。此外,甘草和甘草酸的保护主要肝细胞对咪唑硫嘌呤(1<年代vgheight="9.6750002" id="M1" style="vertical-align:-2.29482pt;width:9.6374998px;" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.675)"> <g transform="translate(72,-64.26)"> <text transform="matrix(1,0,0,-1,-71.95,66.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> </text> </g> </g> </svg>米)损伤,肝脏免疫抑制剂药物的副作用(<一个href="#B9">9</一个>]。甘草酸在日本也一直在临床上用于治疗慢性肝炎(<一个href="#B10">10</一个>]。甘草提取物有抗炎活性和抑制培养的肝癌细胞(Hep-3B)扩散<一个href="#B3">3</一个>]。甘草萃取也减少前列腺特异抗原释放,并降低前列腺癌培养细胞系(LNCaP)扩散<一个href="#B11">11</一个>)和七个成分之一的药草补充剂PC-SPES [<一个href="#B12">12</一个>]。</gydF4y2Bap> <p>人参根皂苷含量与广泛的相关生物活性如抗糖尿病(<一个href="#B13">13</一个>,<gydF4y2Ba一个href="#B14">14</一个>),免疫刺激(<一个href="#B15">15</一个>,<gydF4y2Ba一个href="#B16">16</一个>),能够抑制各种培养的癌细胞的生长(<一个href="#B5">5</一个>,<gydF4y2Ba一个href="#B17">17</一个>,<gydF4y2Ba一个href="#B18">18</一个>]。的精确机制归因于人参,人参皂苷细胞细胞毒性并不完全清楚,但与细胞膜的相互作用导致膜渗透之前已经报道过(<一个href="#B18">18</一个>]。</gydF4y2Bap> <p>本研究的目的是确定西洋参的组合(<我>人参quinquefolius</我>)和甘草提取物(<我>乌拉尔甘草</我>实现协同)或反对地影响肝癌细胞株培养的可行性(Hep-G2)。isobolographic分析是利用有效地描述协同,敌对和添加剂反应Hep-G2细胞。Isobolographic分析提供了一个清晰的图形视图的两个组件之间的交互,用来描述两种药物组合之间的相互作用(<一个href="#B19">19</一个>]。</gydF4y2Bap> <h4 id="methods">2。方法</gydF4y2Bah4> <h5 id="sec2.1">2.1。植物材料</gydF4y2Bah5> <p>干人参(<我>人参quinquefolius</我>)、甘草(<我>乌拉尔甘草</我>)的根在当地采购(新加坡)。人参皂苷高效液相色谱标准(Rg1、再保险、Rb1 Rc, Rd)买来Chromadex Inc .(美国加利福尼亚州圣安娜)和甘草酸标准从σ(Steinheim、德国),购买三氟乙酸(组织)是来自默克公司(美国新泽西州怀特豪斯站)。乙腈和甲醇的高效液相色谱级(Tedia Inc .)、费尔菲尔德,美国),乙醇为分析纯(费舍尔科学、英国)。</gydF4y2Bap> <p>干人参根(<我>人参quinquefolius</我>)是磨成粉,在甲醇回流(500毫升)3 h,过滤两次(绘画纸没有4)和提取重复了三次。解决方案集中在真空下,预先处理,提取应用于高分子吸水安伯来特XAD-4(σ,圣路易斯,密苏里州,美国)列(40孔隙直径、床60厘米的体积<年代up>3</年代up>10毫升/分钟的流量),用蒸馏水洗净(1升)删除极性化合物如前所述[<一个href="#B20">20.</一个>]。皂苷含量从列筛选了使用绝对乙醇(500毫升)和集中在真空下,冻干,这里被称为人参提取物(GE)。</gydF4y2Bap> <p>粉干甘草(<我>乌拉尔甘草</我>)的根长为2.5 h甲醇回流(70%,400毫升),两次过滤,提取三次,集中在真空和在此被称为甘草萃取(LE)。</gydF4y2Bap> <h5 id="sec2.2">2.2。高效液相色谱分析</gydF4y2Bah5> <p>水高效液相色谱(米尔福德,妈,美国)装有四元梯度泵和一个二极管阵列(PDA)探测器是用来评估的皂苷含量或各自提取甘草酸。使用反相高效液相色谱分析进行了C18柱(C18, 4.6×250毫米,5<年代vgheight="9.6750002" id="M2" style="vertical-align:-2.29482pt;width:9.6374998px;" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.675)"> <g transform="translate(72,-64.26)"> <text transform="matrix(1,0,0,-1,-71.95,66.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> </text> </g> </g> </svg>米粒子尺寸、水域)的柱温25°C,注入20卷<年代vgheight="9.6750002" id="M3" style="vertical-align:-2.29482pt;width:9.6374998px;" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.675)"> <g transform="translate(72,-64.26)"> <text transform="matrix(1,0,0,-1,-71.95,66.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> </text> </g> </g> </svg>L及人参皂苷检测波长203 nm和254 nm。通用电气、皂苷含量标准,LE和GA分别溶解在甲醇,通过0.45过滤<年代vgheight="9.6750002" id="M4" style="vertical-align:-2.29482pt;width:9.6374998px;" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.675)"> <g transform="translate(72,-64.26)"> <text transform="matrix(1,0,0,-1,-71.95,66.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> </text> </g> </g> </svg>m注射器过滤器(Minisart、德国),之前的分析。为通用电气由两个单独的梯度高效液相色谱法分析程序(称为梯度1和2),由于人参皂苷类似的保留时间Rg1和再保险。溶剂项目包括水的组合(A)和乙腈(B),梯度程序1(乙腈比例和流速是括在括号)是:上市时间0 (20% B, 1毫升/分钟),20分钟(25% B, 0.7毫升/分钟),40分钟(49% B, 0.7毫升/分钟),50分钟(100% B, 0.7毫升/分钟),和60分钟(80% B, 0.7毫升/分钟)。时间梯度程序2包括:0 (20% B, 1毫升/分钟),45分钟22% B, 0.7毫升/分钟),50分钟,(60% B, 0.7毫升/分钟),60分钟(20% B, 0.7毫升/分钟)。</gydF4y2Bap> <p>高效液相色谱法分离梯度LE和GA利用0.5%组织水(A)和乙腈(B),如下:时间0 B(20%), 5分钟B(40%), 10 - 20分钟(50% B),流量是恒定在0.8毫升/分钟。再保险,人参皂苷Rg1 Rb1、Rc、Rd在通用电气和GA勒根据各自的标准曲线,并评估了一式三份。</gydF4y2Bap> <h5 id="sec2.3">2.3。细胞培养</gydF4y2Bah5> <p>人类肝癌(Hep-G2)细胞从美国购买类型文化集合(美国弗吉尼亚州马纳萨斯)。这些细胞被维护在杜尔贝科修改鹰的媒介(DMEM)补充10%胎牛血清(σ),100 U青霉素,和100年<年代vgheight="9.6750002" id="M5" style="vertical-align:-2.29482pt;width:9.6374998px;" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.675)"> <g transform="translate(72,-64.26)"> <text transform="matrix(1,0,0,-1,-71.95,66.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> </text> </g> </g> </svg>g / mL链霉素(Gibco、表达载体、加拿大)在湿润的气氛中5%的有限公司<年代ub>2</年代ub>在37°C。细胞之间的浓度维持在2×10<年代up>5</年代up>和1×10<年代up>6</年代up>细胞/毫升。细胞每2 - 3天亚文化总介质替代使用0.25% (w / v)胰蛋白酶−0.53毫米EDTA溶液(GIBCO)。可行的细胞被评估0.04%台盼蓝排斥染料(MP生物医学,梭伦,哦,美国)使用血细胞计数器和评估一式四份。</gydF4y2Bap> <h5 id="sec2.4">2.4。细胞MTT分析可行性</gydF4y2Bah5> <p>细胞生存能力是衡量3 - (4 5-dimethylthiazol-2-yl) 2, 5-diphenyl溴化四唑,MTT(σ)试验,为了建立一个LC50值(浓度抑制细胞的50%)。细胞被播种在1×10的浓度<年代up>5</年代up>细胞/在96 - microwell板和被允许坚持24 h。股票的解决方案的通用电气和勒(1.0毫克/毫升)溶解和过滤无菌0.2<年代vgheight="9.6750002" id="M6" style="vertical-align:-2.29482pt;width:9.6374998px;" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.675)"> <g transform="translate(72,-64.26)"> <text transform="matrix(1,0,0,-1,-71.95,66.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> </text> </g> </g> </svg>m注射器过滤器(Millex GP,爱尔兰)前增加了细胞。提取物添加不同浓度(0.2 - -0.9毫克/毫升)和孵化72 h。控件包含Hep-G2细胞培养基,但没有提取物。72年底,h,含有介质被100人<年代vgheight="9.6750002" id="M7" style="vertical-align:-2.29482pt;width:9.6374998px;" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.675)"> <g transform="translate(72,-64.26)"> <text transform="matrix(1,0,0,-1,-71.95,66.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> </text> </g> </g> </svg>肝癌和L(0.5毫克/毫升的添加和孵化4 h如前所述<一个href="#B21">21</一个>100年),晶体是可溶性<年代vgheight="9.6750002" id="M8" style="vertical-align:-2.29482pt;width:9.6374998px;" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.675)"> <g transform="translate(72,-64.26)"> <text transform="matrix(1,0,0,-1,-71.95,66.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> </text> </g> </g> </svg>L 10% SDS(新加坡国立大学的医疗机构)一夜之间在0.01 N盐酸和孵化。吸光度测量在550 nm使用标(Multiskan光谱,热电子公司,沃尔瑟姆,妈,美国),结果被表示为可行的细胞对未经治疗的比例控制细胞。细胞生存能力(%)是计算(平均吸光度样品/控制的平均吸光度)×100。</gydF4y2Bap> <h5 id="sec2.5">2.5。Isobologram分析</gydF4y2Bah5> <p>通用电气和勒之间的交互进行了分析使用一个isobologram和显著性检验的方法Tallarida (2000) (<一个href="#B22">22</一个>]。上面描述的人参和甘草提取物的LC50值被用来绘制isobologram。通用电气的LC50值绘制的<我>x</gydF4y2Ba我>设在,LC50勒的价值<我>y</gydF4y2Ba我>设在。线连接的两个点被称为的可加性,代表了预测LC50(指(<一个href="#EEq1">1</一个>)提取的不同组合。敌对的组合是点线表面的,而协同组合是点线(以下<一个href="#B22">22</一个>]。所使用的固定比率设计。简单地说,五个分数(1/5,1/3、1/2 2/3,4/5)通用电气勒被用来测试的交互两个提取物(指(<一个href="#EEq2">2</一个>))。LC50值首次获得了通用电气和LE独自然后LC50值获得五分数。</gydF4y2Bap> <h5 id="sec2.6">2.6。细胞LDH活性</gydF4y2Bah5> <p>Hep-G2细胞浓度的5×10被播种<年代up>5</年代up>细胞/毫升24-well盘子和允许坚持24 h。中被删除和提取(人参、甘草或五个分数)在各自的LC50浓度添加到井中。使用的浓度为0.64毫克/毫升(GE), 0.53毫克/毫升(LE), 0.64毫克/毫升(f1/5), 0.70毫克/毫升(f1/3), 0.74毫克/毫升(f1/2), 0.74毫克/毫升(f2/3)和0.69毫克/毫升(f4/5)。细胞培养24、48和72 h,和未经处理的细胞作为控制。在每个孵化时间,介质被和检测LDH活性如前所述<一个href="#B5">5</一个>]。</gydF4y2Bap> <h5 id="sec2.7">2.7。细胞循环分析</gydF4y2Bah5> <p>通用电气、勒和五个分数(1/5,1/3、1/2 2/3,4/5)被添加在各自LC50 Hep-G2细胞浓度。细胞培养24、48和72 h与未经处理的细胞作为控制。细胞循环分析确定如前所述[<一个href="#B21">21</一个>]。短暂,细胞使胰蛋白酶化,用PBS和细胞颗粒固定在冰冷的70%乙醇在一夜之间在4°C。上层清液被离心,1包含50毫升的PBS<年代vgheight="9.6750002" id="M9" style="vertical-align:-2.29482pt;width:9.6374998px;" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,9.675)"> <g transform="translate(72,-64.26)"> <text transform="matrix(1,0,0,-1,-71.95,66.6)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> </text> </g> </g> </svg>g / mL propidium碘(σ)和100 U /毫升核糖核酸酶(美国Applichem Inc .,柴郡,CT)在室温下被添加和孵化前1 h数据采集使用Dako Cytomation青色LX流式细胞仪(美国贝克曼库尔特,富勒顿,CA)和分析Dako峰会v4.3软件包。</gydF4y2Bap> <h5 id="sec2.8">2.8。统计和数据分析</gydF4y2Bah5> <p>通用电气的LC50值,LE和五个分数决定从五个不同的五个复制实验为每个实验。LDH和细胞循环分析和三个复制重复三次。数据表示为平均值±标准偏差(SD)。单向方差分析与图基事后比较意味着被用来测试的意义(<年代vgheight="11.25" id="M10" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>)的LDH和细胞循环使用SPSS统计分析软件(美国v12.0,芝加哥,IL)。</gydF4y2Bap> <p><span class="statement" id="head1"><span class="statement-title"><i class="statement-title">确定理论LC50 (CAL50)</我></年代pan><br><span class="equation" id="EEq1"> <svg height="16.5375" id="M11" preserveaspectratio="xMaxYMin" style="vertical-align:-0pt;width:700.9375px;" version="1.1" viewbox="0 0 700.9375 16.5375" width="700.9375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,16.5375)"> <g transform="translate(72,-58.77)"> <text transform="matrix(1,0,0,-1,135.1,63.2)"> <tspan style="font-size: 12.50px; " x="0" y="0"> C</t年代pan> <tspan style="font-size: 12.50px; " x="8.1519556" y="0"> 一个</t年代pan> <tspan style="font-size: 12.50px; " x="17.179123" y="0"> l</t年代pan> <tspan style="font-size: 12.50px; " x="24.818455" y="0"> 5</t年代pan> <tspan style="font-size: 12.50px; " x="31.069956" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="40.772282" y="0"> =</t年代pan> <tspan style="font-size: 12.50px; " x="52.812672" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="61.252197" y="0"> G</t年代pan> <tspan style="font-size: 12.50px; " x="70.279366" y="0"> E</t年代pan> <tspan style="font-size: 12.50px; " x="80.694359" y="0"> +</t年代pan> <tspan style="font-size: 12.50px; " x="92.047089" y="0"> (</t年代pan> <tspan style="font-size: 12.50px; " x="96.210587" y="0"> 1</t年代pan> <tspan style="font-size: 12.50px; " x="105.23775" y="0"> −</t年代pan> <tspan style="font-size: 12.50px; " x="116.57797" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="125.01749" y="0"> )</t年代pan> <tspan style="font-size: 12.50px; " x="131.269" y="0"> l</t年代pan> <tspan style="font-size: 12.50px; " x="138.90833" y="0"> E</t年代pan> <tspan style="font-size: 12.50px; " x="339.01999" y="0.63"> (</t年代pan> <tspan style="font-size: 12.50px; " x="343.1835" y="0.63"> 1</t年代pan> <tspan style="font-size: 12.50px; " x="349.435" y="0.63"> )</t年代pan> </text> </g> </g> </svg></span><br>通用电气和LE变量代表的LC50实验确定值人参和甘草提取物,和变量<我>f</gydF4y2Ba我>表示修复率分数(1/5,1/3、1/2 2/3,4/5)通用电气的组合,CAL50 LC50理论或计算。</年代pan></p> <p><span class="statement" id="head2"><span class="statement-title"><i class="statement-title">在每个分数测定浓度的个体提取</我></年代pan><br><span class="equation" id="EEq2"> <svg height="64.462502" id="M12" preserveaspectratio="xMaxYMin" style="vertical-align:-0pt;width:700.9375px;" version="1.1" viewbox="0 0 700.9375 64.462502" width="700.9375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,64.4625)"> <g transform="translate(72,-20.43)"> <text transform="matrix(1,0,0,-1,167.67,55.15)"> <tspan style="font-size: 12.50px; " x="0" y="0"> G</t年代pan> <tspan style="font-size: 12.50px; " x="9.0271664" y="0"> E</t年代pan> </text> <text transform="matrix(1,0,0,-1,184.34,52.02)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </t年代pan> </text> <text transform="matrix(1,0,0,-1,194.22,55.15)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</t年代pan> <tspan style="font-size: 12.50px; " x="19.33" y="-8.3500004"> </t年代pan> <tspan style="font-size: 12.50px; " x="27.769526" y="-8.3500004"> G</t年代pan> <tspan style="font-size: 12.50px; " x="36.796692" y="-8.3500004"> E</t年代pan> </text> <g> <path d="m 207.45,58.27 37.31,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path> </g> <text transform="matrix(1,0,0,-1,207.45,46.39)"> <tspan style="font-size: 12.50px; " x="38.5" y="-8.7600002"> ,</t年代pan> <tspan style="font-size: 12.50px; " x="0" y="0"> C</t年代pan> <tspan style="font-size: 12.50px; " x="8.1394529" y="0"> 一个</t年代pan> <tspan style="font-size: 12.50px; " x="17.166618" y="0"> l</t年代pan> <tspan style="font-size: 12.50px; " x="24.805952" y="0"> 5</t年代pan> <tspan style="font-size: 12.50px; " x="31.057451" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="-46.119999" y="16.98"> l</t年代pan> <tspan style="font-size: 12.50px; " x="-38.480667" y="16.98"> E</t年代pan> </text> <text transform="matrix(1,0,0,-1,176.61,26.28)"> <tspan style="font-size: 8.75px; " x="0" y="0"> </t年代pan> </text> <text transform="matrix(1,0,0,-1,186.49,29.41)"> <tspan style="font-size: 12.50px; " x="0" y="0"> =</t年代pan> <tspan style="font-size: 12.50px; " x="13.23" y="-8.3599997"> (</t年代pan> <tspan style="font-size: 12.50px; " x="17.393499" y="-8.3599997"> 1</t年代pan> <tspan style="font-size: 12.50px; " x="26.420666" y="-8.3599997"> −</t年代pan> <tspan style="font-size: 12.50px; " x="37.760887" y="-8.3599997"> </t年代pan> <tspan style="font-size: 12.50px; " x="46.200413" y="-8.3599997"> )</t年代pan> <tspan style="font-size: 12.50px; " x="52.451912" y="-8.3599997"> l</t年代pan> <tspan style="font-size: 12.50px; " x="60.091244" y="-8.3599997"> E</t年代pan> </text> <g> <path d="m 199.72,32.53 54.5,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path> </g> <text transform="matrix(1,0,0,-1,208.32,20.66)"> <tspan style="font-size: 12.50px; " x="0" y="0"> C</t年代pan> <tspan style="font-size: 12.50px; " x="8.1394529" y="0"> 一个</t年代pan> <tspan style="font-size: 12.50px; " x="17.166618" y="0"> l</t年代pan> <tspan style="font-size: 12.50px; " x="24.805952" y="0"> 5</t年代pan> <tspan style="font-size: 12.50px; " x="31.057451" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="265.79999" y="-22.43"> (</t年代pan> <tspan style="font-size: 12.50px; " x="269.9635" y="-22.43"> 2</t年代pan> <tspan style="font-size: 12.50px; " x="276.215" y="-22.43"> )</t年代pan> </text> </g> </g> </svg></span><br>变量<我>f</gydF4y2Ba我>通用电气,LE和CAL50上面所描述的那样,通用电气<我><年代ub>f</年代ub></i>和勒<我><年代ub>f</年代ub></i>代表每个根提取物添加量每个分数。</年代pan></p> <p><span class="statement" id="head3"><span class="statement-title"><i class="statement-title">添加剂、协同作用和对抗</我></年代pan><br><span class="equation" id="EEq4"> <svg height="59.462502" id="M13" preserveaspectratio="xMaxYMin" style="vertical-align:-0pt;width:700.9375px;" version="1.1" viewbox="0 0 700.9375 59.462502" width="700.9375" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,59.4625)"> <g transform="translate(72,-24.43)"> <text transform="matrix(1,0,0,-1,120.28,63.2)"> <tspan style="font-size: 12.50px; " x="0" y="0"> 一个</t年代pan> <tspan style="font-size: 12.50px; " x="8.8271179" y="0"> d</t年代pan> <tspan style="font-size: 12.50px; " x="15.078618" y="0"> d</t年代pan> <tspan style="font-size: 12.50px; " x="21.330118" y="0"> 我</t年代pan> <tspan style="font-size: 12.50px; " x="24.805952" y="0"> t</t年代pan> <tspan style="font-size: 12.50px; " x="28.281786" y="0"> 我</t年代pan> <tspan style="font-size: 12.50px; " x="31.75762" y="0"> v</t年代pan> <tspan style="font-size: 12.50px; " x="37.709049" y="0"> e</t年代pan> <tspan style="font-size: 12.50px; " x="43.26038" y="0"> :</t年代pan> <tspan style="font-size: 12.50px; " x="50.887211" y="0"> C</t年代pan> <tspan style="font-size: 12.50px; " x="59.039165" y="0"> 一个</t年代pan> <tspan style="font-size: 12.50px; " x="68.06633" y="0"> l</t年代pan> <tspan style="font-size: 12.50px; " x="75.705666" y="0"> 5</t年代pan> <tspan style="font-size: 12.50px; " x="81.957169" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="90.971825" y="0"> −</t年代pan> <tspan style="font-size: 12.50px; " x="102.32455" y="0"> E</t年代pan> <tspan style="font-size: 12.50px; " x="109.96388" y="0"> X</t年代pan> <tspan style="font-size: 12.50px; " x="118.99105" y="0"> P</t年代pan> <tspan style="font-size: 12.50px; " x="125.95522" y="0"> l</t年代pan> <tspan style="font-size: 12.50px; " x="133.59456" y="0"> C</t年代pan> <tspan style="font-size: 12.50px; " x="141.93405" y="0"> 5</t年代pan> <tspan style="font-size: 12.50px; " x="148.18556" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="157.88788" y="0"> =</t年代pan> <tspan style="font-size: 12.50px; " x="169.92827" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="11.13" y="17.49"> 年代</t年代pan> <tspan style="font-size: 12.50px; " x="18.081669" y="17.49"> y</t年代pan> <tspan style="font-size: 12.50px; " x="24.333168" y="17.49"> n</t年代pan> <tspan style="font-size: 12.50px; " x="30.584667" y="17.49"> e</t年代pan> <tspan style="font-size: 12.50px; " x="36.136002" y="17.49"> r</t年代pan> <tspan style="font-size: 12.50px; " x="40.099449" y="17.49"> g</t年代pan> <tspan style="font-size: 12.50px; " x="46.350952" y="17.49"> y</t年代pan> <tspan style="font-size: 12.50px; " x="52.502426" y="17.49"> :</t年代pan> <tspan style="font-size: 12.50px; " x="60.141762" y="17.49"> E</t年代pan> <tspan style="font-size: 12.50px; " x="67.78109" y="17.49"> X</t年代pan> <tspan style="font-size: 12.50px; " x="76.808258" y="17.49"> P</t年代pan> <tspan style="font-size: 12.50px; " x="83.77243" y="17.49"> l</t年代pan> <tspan style="font-size: 12.50px; " x="91.411766" y="17.49"> C</t年代pan> <tspan style="font-size: 12.50px; " x="99.751266" y="17.49"> 5</t年代pan> <tspan style="font-size: 12.50px; " x="106.00276" y="17.49"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="115.70509" y="17.49"> <</t年代pan> <tspan style="font-size: 12.50px; " x="127.74548" y="17.49"> C</t年代pan> <tspan style="font-size: 12.50px; " x="135.89743" y="17.49"> 一个</t年代pan> <tspan style="font-size: 12.50px; " x="144.92461" y="17.49"> l</t年代pan> <tspan style="font-size: 12.50px; " x="152.56393" y="17.49"> 5</t年代pan> <tspan style="font-size: 12.50px; " x="158.81543" y="17.49"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="1.1900001" y="34.970001"> 一个</t年代pan> <tspan style="font-size: 12.50px; " x="10.217166" y="34.970001"> n</t年代pan> <tspan style="font-size: 12.50px; " x="16.468666" y="34.970001"> t</t年代pan> <tspan style="font-size: 12.50px; " x="20.057028" y="34.970001"> 一个</t年代pan> <tspan style="font-size: 12.50px; " x="25.608358" y="34.970001"> g</t年代pan> <tspan style="font-size: 12.50px; " x="31.859859" y="34.970001"> o</t年代pan> <tspan style="font-size: 12.50px; " x="38.111359" y="34.970001"> n</t年代pan> <tspan style="font-size: 12.50px; " x="44.362858" y="34.970001"> 我</t年代pan> <tspan style="font-size: 12.50px; " x="47.838692" y="34.970001"> 年代</t年代pan> <tspan style="font-size: 12.50px; " x="52.702358" y="34.970001"> 米</t年代pan> <tspan style="font-size: 12.50px; " x="62.429695" y="34.970001"> :</t年代pan> <tspan style="font-size: 12.50px; " x="70.081528" y="34.970001"> E</t年代pan> <tspan style="font-size: 12.50px; " x="77.720863" y="34.970001"> X</t年代pan> <tspan style="font-size: 12.50px; " x="86.748032" y="34.970001"> P</t年代pan> <tspan style="font-size: 12.50px; " x="93.712196" y="34.970001"> l</t年代pan> <tspan style="font-size: 12.50px; " x="101.35153" y="34.970001"> C</t年代pan> <tspan style="font-size: 12.50px; " x="109.69103" y="34.970001"> 5</t年代pan> <tspan style="font-size: 12.50px; " x="115.94254" y="34.970001"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="125.64486" y="34.970001"> ></t年代pan> <tspan style="font-size: 12.50px; " x="137.68526" y="34.970001"> C</t年代pan> <tspan style="font-size: 12.50px; " x="145.8372" y="34.970001"> 一个</t年代pan> <tspan style="font-size: 12.50px; " x="154.86438" y="34.970001"> l</t年代pan> <tspan style="font-size: 12.50px; " x="162.50371" y="34.970001"> 5</t年代pan> <tspan style="font-size: 12.50px; " x="168.7552" y="34.970001"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="353.84" y="18.110001"> (</t年代pan> <tspan style="font-size: 12.50px; " x="358.00351" y="18.110001"> 3</t年代pan> <tspan style="font-size: 12.50px; " x="364.255" y="18.110001"> )</t年代pan> </text> </g> </g> </svg></span><br>变量EXPLC50分数和变量的实验派生LC50 CAL50上面描述。测试的意义和上面的方程是基于文献报道[<一个href="#B22">22</一个>,<gydF4y2Ba一个href="#B23">23</一个>]。</年代pan></p> <h4 id="results">3所示。结果</gydF4y2Bah4> <h5 id="sec3.1">3.1。高效液相色谱分析</gydF4y2Bah5> <p>个人人参皂苷分析表明,通用电气的皂苷含量的百分比确定为(20.2±7.8),Rb1 (8.8±0.2), Rg1(1.5±0.04)和Rd(0.71±0.01),和总皂苷含量的内容确定为30.9±7.8%(干重)。人参皂苷Rc检测但低于检测极限。对勒,甘草酸的总比例是确定为7.1±0.2%(干重)。</gydF4y2Bap> <h5 id="sec3.2">3.2。剂量反应LC50测定人参和甘草提取物</gydF4y2Bah5> <p>图<一个href="//www.newsama.com/journals/ecam/2011/408273/fig1/" target="_blank">1</一个>显示了通用电气和勒的剂量反应关系。LC50计算通过绘制细胞生存能力(%)和日志集中(图中没有显示),产生一个线性方程<我>y</gydF4y2Ba我>=−164。32<gydF4y2Ba我>x</gydF4y2Ba我>+ 511.58 (<gydF4y2Ba我>r</gydF4y2Ba我><年代up>2</年代up>通用电气和= 0.982)<我>y</gydF4y2Ba我>=−218。84<gydF4y2Ba我>x</gydF4y2Ba我>+ 646.88 (<gydF4y2Ba我>r</gydF4y2Ba我><年代up>2</年代up>勒= 0.981)。lc50被确定为0.64±0.02毫克/毫升为通用电气和0.53±0.02毫克/毫升勒。通用电气和LE显示剂量依赖效应Hep-G2生存能力,和LE LC50价值被发现显著(<年代vgheight="11.25" id="M14" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>比通用电气)低毒杀。</gydF4y2Bap> <div class="partial-carousel-wrapper"> <div class="floats-partial-section"> <div class="floats-partial-content"> <div class="partial-carousel--full-image partial-carousel-877f2e01cf9f788624cec1825bb6a24b"> <div class="partial-carousel--single-image"> <img loading="lazy" alt="" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.001.jpg"> <br> <strong></strong> </div> </div> </div> <div class="floats-partial-middle"> <div class="floats-partial-middle--thumbs"> <div class="partial-carousel-dots-877f2e01cf9f788624cec1825bb6a24b partial-carousel-thumbnail"> <button aria-label="Slide to " class="partial-carousel-dot"><img loading="lazy" alt="" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.001_th.jpg"><br><strong></strong></button> </div> </div> <div class="floats-partial-middle--nav"> <div class="partial-carousel-next-prev-877f2e01cf9f788624cec1825bb6a24b partial-carousel-next-prev"> <button aria-label="Previous" class="carousel-prev"></button> <button aria-label="Next" class="carousel-next"></button> </div> </div> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">图1</年代pan> <a aria-label="View full page" class="caption-partial-url" href="//www.newsama.com/journals/ecam/2011/408273/fig1/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> <table> <tbody> <tr> <td>通用电气和LE Hep-G2生存能力的影响。结果表示为平均数±标准差的五个独立实验五个复制。</td></tr></tbody> </table> </div> </div> </div> </div> <h5 id="sec3.3">3.3。剂量反应LC50确定分数</gydF4y2Bah5> <p>五分数对应的LC50值比人参的甘草提取物的1/5,1/3、1/2,2/3和4/5和图所示<一个href="//www.newsama.com/journals/ecam/2011/408273/fig2/" target="_blank">2(一个)</一个>- - - - - -<一个href="//www.newsama.com/journals/ecam/2011/408273/fig2/" target="_blank">2 (e)</一个>)和计算如上所述。通用电气的LC50相比,分数(1/3、1/2 2/3,4/5)都显著(<年代vgheight="11.25" id="M15" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>)大于通用除了f1/5类似于通用电气。此外,所有分数有显著的LC50 (<年代vgheight="11.25" id="M16" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>相比)更大的LC50勒(图<一个href="//www.newsama.com/journals/ecam/2011/408273/fig2/" target="_blank">2 (f)</一个>)。</gydF4y2Bap> <div class="partial-carousel-wrapper"> <div class="floats-partial-section"> <div class="floats-partial-content"> <div class="partial-carousel--full-image partial-carousel-a90d93a53866185cb6ccc7646bb4cd3d"> <div class="partial-carousel--single-image"> <img loading="lazy" alt="(一)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.002a.jpg"> <br> <strong>(一)</年代trong></d我v> <div class="partial-carousel--single-image"> <img loading="lazy" alt="(b)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.002b.jpg"> <br> <strong>(b)</年代trong></d我v> <div class="partial-carousel--single-image"> <img loading="lazy" alt="(c)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.002c.jpg"> <br> <strong>(c)</年代trong></d我v> <div class="partial-carousel--single-image"> <img loading="lazy" alt="(d)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.002d.jpg"> <br> <strong>(d)</年代trong></d我v> <div class="partial-carousel--single-image"> <img loading="lazy" alt="(e)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.002e.jpg"> <br> <strong>(e)</年代trong></d我v> <div class="partial-carousel--single-image"> <img loading="lazy" alt="(f)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.002f.jpg"> <br> <strong>(f)</年代trong></d我v> </div> </div> <div class="floats-partial-middle"> <div class="floats-partial-middle--thumbs"> <div class="partial-carousel-dots-a90d93a53866185cb6ccc7646bb4cd3d partial-carousel-thumbnail"> <button aria-label="Slide to (a) " class="partial-carousel-dot"><img loading="lazy" alt="(一)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.002a_th.jpg"><br><strong>(一)</年代trong></button> <button aria-label="Slide to (b) " class="partial-carousel-dot"><img loading="lazy" alt="(b)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.002b_th.jpg"><br><strong>(b)</年代trong></button> <button aria-label="Slide to (c) " class="partial-carousel-dot"><img loading="lazy" alt="(c)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.002c_th.jpg"><br><strong>(c)</年代trong></button> <button aria-label="Slide to (d) " class="partial-carousel-dot"><img loading="lazy" alt="(d)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.002d_th.jpg"><br><strong>(d)</年代trong></button> <button aria-label="Slide to (e) " class="partial-carousel-dot"><img loading="lazy" alt="(e)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.002e_th.jpg"><br><strong>(e)</年代trong></button> <button aria-label="Slide to (f) " class="partial-carousel-dot"><img loading="lazy" alt="(f)gydF4y2Ba" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.002f_th.jpg"><br><strong>(f)</年代trong></button> </div> </div> <div class="floats-partial-middle--nav"> <div class="partial-carousel-next-prev-a90d93a53866185cb6ccc7646bb4cd3d partial-carousel-next-prev"> <button aria-label="Previous" class="carousel-prev"></button> <button aria-label="Next" class="carousel-next"></button> </div> </div> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">图2</年代pan> <a aria-label="View full page" class="caption-partial-url" href="//www.newsama.com/journals/ecam/2011/408273/fig2/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> <table> <tbody> <tr> <td>五个不同分数的影响(1/5,1/3、1/2 2/3,4/5)在Hep-G2可行性(一)- (e)。(f)对应于通用电气的lc50值,LE和五个分数。酒吧有不同的字母明显不同(<年代vgheight="11.375" id="M17" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 50.549999 11.375" width="50.549999" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"> <g transform="matrix(.017,-0,0,-.017,.062,11.112)"> <path d="M619 482q0 -69 -40.5 -119t-96 -72.5t-118.5 -26.5h-44l-70 20l-31 -151q-14 -67 0.5 -83t89.5 -22l-5 -28h-287l8 28q65 7 81.5 22t29.5 83l79 398q12 56 0.5 70.5t-78.5 20.5l7 28h255q108 0 164 -43t56 -125zM524 478q0 141 -146 141q-25 0 -47 -8q-16 -6 -20.5 -13.5 t-10.5 -39.5l-43 -241q37 -13 83 -13q67 0 125.5 45t58.5 129z" id="x1D443"></path> </g> <g transform="matrix(.017,-0,0,-.017,15.582,11.112)"> <path d="M512 -3l-437 233v51l437 233v-58l-378 -200v-2l378 -199v-58z" id="x3C"></path> </g> <g transform="matrix(.017,-0,0,-.017,30.286,11.112)"> <path d="M113 -12q-24 0 -39.5 16t-15.5 42q0 24 16 40.5t40 16.5t40 -16.5t16 -40.5q0 -26 -16 -42t-41 -16z" id="x2E"></path> </g> <g transform="matrix(.017,-0,0,-.017,34.162,11.112)"> <path d="M241 635q53 0 94 -28.5t63.5 -76t33.5 -102.5t11 -116q0 -58 -11 -112.5t-34 -103.5t-63.5 -78.5t-94.5 -29.5t-95 28t-64.5 75t-34.5 102.5t-11 118.5q0 58 11.5 112.5t34.5 103t64.5 78t95.5 29.5zM238 602q-32 0 -55.5 -25t-35.5 -68t-17.5 -91t-5.5 -105 q0 -76 10 -138.5t37 -107.5t69 -45q32 0 55.5 25t35.5 68.5t17.5 91.5t5.5 105t-5.5 105.5t-18 92t-36 68t-56.5 24.5z" id="x30"></path> </g> <g transform="matrix(.017,-0,0,-.017,42.321,11.112)"> <path d="M153 550l-26 -186q79 31 111 31q90 0 141.5 -51t51.5 -119q0 -93 -89 -166q-85 -69 -173 -71q-32 0 -61.5 11.5t-41.5 23.5q-18 17 -17 34q2 16 22 33q14 9 26 -1q61 -50 124 -50q60 0 93 43.5t33 104.5q0 69 -41.5 110t-121.5 41q-53 0 -102 -20l38 305h286l6 -8 l-26 -65h-233z" id="x35"></path> </g> </svg>)。结果表示为平均数±标准差的五个独立实验五个复制。</td></tr></tbody> </table> </div> </div> </div> </div> <h5 id="sec3.4">3.4。Isobolographic分析</gydF4y2Bah5> <p>图<一个href="//www.newsama.com/journals/ecam/2011/408273/fig3/" target="_blank">3</一个>代表的图形视图的影响通用电气和LE Hep-G2细胞生存能力的组合。创建一个isobologram使用通用电气的LC50值,这相交<我>x</gydF4y2Ba我>勒的毒杀设在价值,相交<我>y</gydF4y2Ba我>设在。这两个点是连接在一起形成的可加性。这行相加性指的是通用电气和LE提取需要的浓度产生同样的效果,如果他们单独行动。点躺在上面的线称为添加剂,这条线是敌对的,线下是协同(<一个href="#B22">22</一个>,<gydF4y2Ba一个href="#B23">23</一个>]。所有组合(1/5,1/3、1/2,2/3和4/5)的通用电气和LE显示对抗。他们都大于可加性。分数1/2和2/3显示最大的拮抗效应。表<一个href="//www.newsama.com/journals/ecam/2011/408273/tab1/" target="_blank">1</一个>显示了LC50 LC50理论和实验观察。分数1/3和1/2的差距最大理论LC50 23岁和21%,分别。</gydF4y2Bap> <div class="floats-partial-section partial-other-wrapper" data-section="tab1"> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">表1</年代pan> <a aria-label="View full page" class="caption-partial-url" href="//www.newsama.com/journals/ecam/2011/408273/tab1/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> 理论和观察人参和甘草提取物的LC50值。</d我v><一个class="table-thumb-overlay" href="//www.newsama.com/journals/ecam/2011/408273/tab1" target="_blank" aria-label="View full page"></a> </div> </div> <div class="partial-carousel-wrapper"> <div class="floats-partial-section"> <div class="floats-partial-content"> <div class="partial-carousel--full-image partial-carousel-dd5b6d3d7064ce0b5fe891c2c5d14267"> <div class="partial-carousel--single-image"> <img loading="lazy" alt="" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.003.jpg"> <br> <strong></strong> </div> </div> </div> <div class="floats-partial-middle"> <div class="floats-partial-middle--thumbs"> <div class="partial-carousel-dots-dd5b6d3d7064ce0b5fe891c2c5d14267 partial-carousel-thumbnail"> <button aria-label="Slide to " class="partial-carousel-dot"><img loading="lazy" alt="" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.003_th.jpg"><br><strong></strong></button> </div> </div> <div class="floats-partial-middle--nav"> <div class="partial-carousel-next-prev-dd5b6d3d7064ce0b5fe891c2c5d14267 partial-carousel-next-prev"> <button aria-label="Previous" class="carousel-prev"></button> <button aria-label="Next" class="carousel-next"></button> </div> </div> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">图3</年代pan> <a aria-label="View full page" class="caption-partial-url" href="//www.newsama.com/journals/ecam/2011/408273/fig3/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> <table> <tbody> <tr> <td>Isobolographic表示之间的交互实验测量通用电气,LE分数(图形点)和Hep-G2理论线加法(实线)的可行性。虚线代表的理论标准偏差+ / - 2.5%。实验分和误差表示为平均数±标准差用五个复制五个独立的实验。</td></tr></tbody> </table> </div> </div> </div> </div> <h5 id="sec3.5">3.5。细胞循环分析</gydF4y2Bah5> <p>代表DNA直方图的各种治疗方法如图所示<一个href="//www.newsama.com/journals/ecam/2011/408273/fig4/" target="_blank">4</一个>和相应的分析是列在表中<一个href="//www.newsama.com/journals/ecam/2011/408273/tab2/" target="_blank">2</一个>。通常不被观察凋亡细胞G1(子)在细胞循环分析。大部分的分数测试没有任何明显的凋亡细胞的增加在24小时的治疗比未经处理的控制。通用电气显著(<年代vgheight="11.25" id="M19" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>)增加子G1细胞但增加只有边际48和72 h。总的来说,Hep-G2细胞通常表现出显著(<年代vgheight="11.25" id="M20" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>)增加在G1期细胞的比例在24和48 h时期除了通用电气在24 h。减少观察G2 / M细胞的比例提取和组合相比,控制。48和72小时的治疗,显著减少(<年代vgheight="11.25" id="M21" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>在G2 / M细胞中观察到的比例分数1/3,1/2,2/3和4/5。在S期细胞观察但不显著减少除了分数1/3,1/2,治疗24小时后2/3和4/5。</gydF4y2Bap> <div class="floats-partial-section partial-other-wrapper" data-section="tab2"> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">表2</年代pan> <a aria-label="View full page" class="caption-partial-url" href="//www.newsama.com/journals/ecam/2011/408273/tab2/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> 细胞循环分析。</d我v><一个class="table-thumb-overlay" href="//www.newsama.com/journals/ecam/2011/408273/tab2" target="_blank" aria-label="View full page"></a> </div> </div> <div class="partial-carousel-wrapper"> <div class="floats-partial-section"> <div class="floats-partial-content"> <div class="partial-carousel--full-image partial-carousel-40d55c65f422866e4d8fe6f2bb8aa8f2"> <div class="partial-carousel--single-image"> <img loading="lazy" alt="" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.004.jpg"> <br> <strong></strong> </div> </div> </div> <div class="floats-partial-middle"> <div class="floats-partial-middle--thumbs"> <div class="partial-carousel-dots-40d55c65f422866e4d8fe6f2bb8aa8f2 partial-carousel-thumbnail"> <button aria-label="Slide to " class="partial-carousel-dot"><img loading="lazy" alt="" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.004_th.jpg"><br><strong></strong></button> </div> </div> <div class="floats-partial-middle--nav"> <div class="partial-carousel-next-prev-40d55c65f422866e4d8fe6f2bb8aa8f2 partial-carousel-next-prev"> <button aria-label="Previous" class="carousel-prev"></button> <button aria-label="Next" class="carousel-next"></button> </div> </div> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">图4</年代pan> <a aria-label="View full page" class="caption-partial-url" href="//www.newsama.com/journals/ecam/2011/408273/fig4/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> <table> <tbody> <tr> <td>Hep-G2细胞的DNA细胞循环直方图。细胞治疗与通用电气,LE和五个分数(1/5,1/3、1/2 2/3,4/5)24、48和72 h,分别在各自的毒杀。未经处理的细胞作为对照组。细胞被固定在70%乙醇和沾染了π节中描述的方法。DNA直方图显示的代表分析在三个独立的实验中也得到了同样的结果。</td></tr></tbody> </table> </div> </div> </div> </div> <h5 id="sec3.6">3.6。LDH分析</gydF4y2Bah5> <p>不同治疗方法的效果和曝光时间LDH释放,膜的完整性和损伤的一个标志,如图<一个href="//www.newsama.com/journals/ecam/2011/408273/fig5/" target="_blank">5</一个>。勒治疗从24到72小时没有明显影响细胞LDH的释放与相应的控制。通用电气治疗LDH释放最大的显著增加,而分数,至少包含一个比例的一半通用电气(如f1/2, f2/3, f4/5)显著(<年代vgheight="11.25" id="M23" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>)增加感染的细胞LDH释放未经处理的控制相比72 h后孵化。分数1/2,2/3,4/5有一个百分比增长57% (f1/2), 192% (f2/3)和263% (f4/5)。通用显示最重要的LDH增加在所有时间里跟着f4/5 48 h和分数f2/3 f4/5。</gydF4y2Bap> <div class="partial-carousel-wrapper"> <div class="floats-partial-section"> <div class="floats-partial-content"> <div class="partial-carousel--full-image partial-carousel-2f8fceb644a7eaeda3525c50329b438b"> <div class="partial-carousel--single-image"> <img loading="lazy" alt="" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/figures/408273.fig.005.jpg"> <br> <strong></strong> </div> </div> </div> <div class="floats-partial-middle"> <div class="floats-partial-middle--thumbs"> <div class="partial-carousel-dots-2f8fceb644a7eaeda3525c50329b438b partial-carousel-thumbnail"> <button aria-label="Slide to " class="partial-carousel-dot"><img loading="lazy" alt="" src="https://static.hindawi.com/articles/ecam/volume-2011/408273/thumbnails/408273.fig.005_th.jpg"><br><strong></strong></button> </div> </div> <div class="floats-partial-middle--nav"> <div class="partial-carousel-next-prev-2f8fceb644a7eaeda3525c50329b438b partial-carousel-next-prev"> <button aria-label="Previous" class="carousel-prev"></button> <button aria-label="Next" class="carousel-next"></button> </div> </div> </div> <div class="floats-partial-footer"> <div class="floats-partial-caption"> <span class="caption-text">图5</年代pan> <a aria-label="View full page" class="caption-partial-url" href="//www.newsama.com/journals/ecam/2011/408273/fig5/" title="" target="_blank"></a> </div> <div class="floats-partial-caption-text"> <table> <tbody> <tr> <td>通用电气的影响,LE和五个分数(1/5,1/3、1/2 2/3,4/5)在LDH活性。细胞治疗不同的提取24、48和72 h各自LC50从72 - h MTT测定浓度决定。结果表示为均值±SD和三个复制重复在三个独立的实验。未经处理的细胞作为对照组。酒吧有不同的字母明显不同(<年代vgheight="11.375" id="M24" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 50.549999 11.375" width="50.549999" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"> <g transform="matrix(.017,-0,0,-.017,.062,11.112)"> <path d="M619 482q0 -69 -40.5 -119t-96 -72.5t-118.5 -26.5h-44l-70 20l-31 -151q-14 -67 0.5 -83t89.5 -22l-5 -28h-287l8 28q65 7 81.5 22t29.5 83l79 398q12 56 0.5 70.5t-78.5 20.5l7 28h255q108 0 164 -43t56 -125zM524 478q0 141 -146 141q-25 0 -47 -8q-16 -6 -20.5 -13.5 t-10.5 -39.5l-43 -241q37 -13 83 -13q67 0 125.5 45t58.5 129z" id="x1D443"></path> </g> <g transform="matrix(.017,-0,0,-.017,15.582,11.112)"> <path d="M512 -3l-437 233v51l437 233v-58l-378 -200v-2l378 -199v-58z" id="x3C"></path> </g> <g transform="matrix(.017,-0,0,-.017,30.286,11.112)"> <path d="M113 -12q-24 0 -39.5 16t-15.5 42q0 24 16 40.5t40 16.5t40 -16.5t16 -40.5q0 -26 -16 -42t-41 -16z" id="x2E"></path> </g> <g transform="matrix(.017,-0,0,-.017,34.162,11.112)"> <path d="M241 635q53 0 94 -28.5t63.5 -76t33.5 -102.5t11 -116q0 -58 -11 -112.5t-34 -103.5t-63.5 -78.5t-94.5 -29.5t-95 28t-64.5 75t-34.5 102.5t-11 118.5q0 58 11.5 112.5t34.5 103t64.5 78t95.5 29.5zM238 602q-32 0 -55.5 -25t-35.5 -68t-17.5 -91t-5.5 -105 q0 -76 10 -138.5t37 -107.5t69 -45q32 0 55.5 25t35.5 68.5t17.5 91.5t5.5 105t-5.5 105.5t-18 92t-36 68t-56.5 24.5z" id="x30"></path> </g> <g transform="matrix(.017,-0,0,-.017,42.321,11.112)"> <path d="M153 550l-26 -186q79 31 111 31q90 0 141.5 -51t51.5 -119q0 -93 -89 -166q-85 -69 -173 -71q-32 0 -61.5 11.5t-41.5 23.5q-18 17 -17 34q2 16 22 33q14 9 26 -1q61 -50 124 -50q60 0 93 43.5t33 104.5q0 69 -41.5 110t-121.5 41q-53 0 -102 -20l38 305h286l6 -8 l-26 -65h-233z" id="x35"></path> </g> </svg>)。</td></tr></tbody> </table> </div> </div> </div> </div> <h4 id="discussion">4所示。讨论</gydF4y2Bah4> <p>有效利用isobolographic分析,我们表明,人参的组合(<我>人参quinquefolius</我>)、甘草(<我>乌拉尔甘草</我>)提取不产生协同效应在减少Hep-G2细胞生存能力。相反,效果是敌对的。分数1/2和2/3被发现LC50值最高(0.75±0.02,0.74±0.02毫克/毫升,职责),相比其他分数和分数1/3和1/2比例最大的差异相比,理论或计算LC50 (CAL50)。亚当斯et al。<一个href="#B19">19</一个>)也报道对抗与组合<我>g。解</我>用<我>测得</我>或<我>年代cuteellaria baicalensis</我>在等量(1:1分数)在前列腺癌细胞培养。<我>乌拉尔甘草,r .得</我>和<我>美国baicalensis</我>也发现在中医处方<一个href="#B24">24</一个>和PC-SPES配方<一个href="#B12">12</一个>]。<我>测得</我>和<我>美国baicalensis</我>已报告,减少培养癌细胞生存能力的细胞系(Hep-G2, MCF-7 MCF-10A) [<一个href="#B25">25</一个>,<gydF4y2Ba一个href="#B26">26</一个>]。</gydF4y2Bap> <p>在这项研究中,人参提取增加了LC50 LDH的释放浓度随时间显著(<年代vgheight="11.25" id="M25" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>)增加曝光的48和72小时,而甘草萃取没有。人参提取物本身已被证明是有效地渗透肠道细胞的细胞膜(int - 407, caco-2细胞)<一个href="#B18">18</一个>)从而从细胞质中LDH释放可能通过与膜胆固醇的交互<一个href="#B27">27</一个>]。值得注意的是,当人参人参甘草含有至少50%的比例(f1/2, f2/3 f4/5)显著(<年代vgheight="11.25" id="M26" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>)增加LDH释放在72 h观察人参和甘草的组合。人参和甘草提取物没有产生任何实质性积累LC50子G1细胞的浓度测试。除了通用电气在24 h, G1期细胞周期的分析表明,有一个重要的(<年代vgheight="11.25" id="M27" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>)细胞循环逮捕所有治疗细胞比控制在24 - 72 h。此外,一个重要的(<年代vgheight="11.25" id="M28" style="vertical-align:-0.30096pt;width:48.337502px;" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.25)"> <g transform="translate(72,-63)"> <text transform="matrix(1,0,0,-1,-71.95,63.35)"> <tspan style="font-size: 12.50px; " x="0" y="0"> </t年代pan> <tspan style="font-size: 12.50px; " x="10.890113" y="0"> <</t年代pan> <tspan style="font-size: 12.50px; " x="22.930502" y="0"> 。</t年代pan> <tspan style="font-size: 12.50px; " x="26.056252" y="0"> 0</t年代pan> <tspan style="font-size: 12.50px; " x="32.307751" y="0"> 5</t年代pan> </text> </g> </g> </svg>)减少在G2 / M期细胞比例被认为在72 h。G1细胞循环逮捕数据与文献报道一致,甘草提取物已报告,抑制细胞增殖,并诱导G1期逮捕的雌激素敏感的乳腺癌细胞系(MCF-7) [<一个href="#B28">28</一个>),影响生存能力培养的前列腺癌细胞(LNCaP) [<一个href="#B11">11</一个>]。</gydF4y2Bap> <p>一个可能的解释这些敌对对细胞活力的影响是人参和甘草提取物的活性化合物可能争夺相同的细胞受体。人参提取物,人参皂苷再保险是最丰富的皂素干重20.2%,紧随其后的是人参皂苷Rb1 (8.8%)、Rg1(1.5%)和Rd (0.7%)。皂苷含量,如Rg1 protopanaxatriol达玛烷型皂苷,包括结构相关的人参皂苷再保险,据报道是糖皮质激素受体的功能配体(<一个href="#B29">29日</一个>)和oleanane-type皂苷类似甘草酸提取已确定为7.1%地塞米松相比,合成糖皮质激素在细胞培养实验中(<一个href="#B30">30.</一个>]。丰富的活性化合物会浸透受体网站会导致低于预期比预测的细胞反应。</gydF4y2Bap> <p>此外,甘草在特定条件下可以表现出hepato-protection。例如,甘草甜素、结构与甘草酸报道减轻腹腔诱导CCl由四氯化碳肝损伤<年代ub>4</年代ub>)在小鼠炎性标记物的监管(<一个href="#B31">31日</一个>]。预处理的培养肝细胞保护甘草和甘草酸对咪唑硫嘌呤损伤通过增加细胞内谷胱甘肽的水平。中村等人报道,甘草甜素导致剂量依赖性抑制CCl LDH泄漏造成的<年代ub>4</年代ub>在初级鼠肝细胞(<一个href="#B32">32</一个>]。这种保护作用是明显的在这个研究中,分数比人参含有更多的甘草减少细胞毒性效应和膜渗透的人参。甘草可能防止人参细胞毒性增加,从而导致观察到的对抗和肝细胞的生存。</gydF4y2Bap> <p>人参的组合使用修复分数isobolographic甘草萃取分析被认为是对抗而不是协同以及个人提取相比,减少了细胞毒性。此外,甘草萃取人参提取物发现降低膜渗透性能。这还需要进一步的研究来确定精确的细胞触发,如果这些观察到的效应细胞特定的。</gydF4y2Bap> <h4 id="funding">资金</gydF4y2Bah4> 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