研究文章

中性粒细胞胞外陷阱在致命COVID-19-Associated肺损伤

图3

疣状低密度的中性粒细胞(LDN)标记CD66b(深蓝色(g))和CD15(布朗(h n)), DNA /核与福尔根氏复染色法(紫色(g))和苏木精(蓝色(h n))。(一)概述图像显示CD66b +细胞的分布;注意城市群在肺泡隔。(b)肺泡间隔由CD66b +细胞浸润(他们中的大多数可能LDNs);扩大椭圆形细胞网状CD66 +馅料也为DNA染色在肺泡空间左上角(箭头所指)。(c)组成的Microthrombus CD66b + LDNs和渔网。(d) NET-forming CD66b +细胞在凝结的毛细管分岔。(e) CD66b +肺泡细胞空间的聚类。(f)广泛的积累和microthrombus CD66b +细胞形成的爸爸;点网的血管壁(箭头)。 (g) Large CD66b+ cell (presumably macrophage) in an alveolar space. (h) Overview image showing the accumulation of CD15+ cells in alveolar septa and the occurrence of such cells in vascular clots (arrows). (i) CD15+ NET-like extracellular material interstratifying an eroded alveolar septum. (j) NET-forming CD15+ cell (arrow) in an arteriolar microthrombus. (k) Top left: microthrombus exclusively formed by CD15+ cells (presumably LDNs); bottom right: “conventional type” microthrombus mainly consisting of anucleated material but interspersed with NETs surrounding a strong CD15+ cell. (l) Massive mural microthrombotic accretion of CD15+ cells in a medium-sized arteriolar vessel with tangentially sectioned wall. (m) CD15+ cells and NETs in proliferative DAD. (n) Alveolar spaces with enlarged ovally shaped CD15+ NET-forming cells (arrows) similar to those shown for MPO and citH3; nearby NETs costain for DNA and CD15 (arrowheads); NETs also abundantly intersperse wall tissues (asterisk). Scale bars: 50 μm。