is more in keeping with intercalators and suggests this binding mode. The viscosity measurements showed that the complex-DNA interaction can be hydrophobic and confirm intercalation. Moreover, the complex induced detectable changes in the CD spectrum of CT-DNA. The fluorescence studies revealed that the probable quenching mechanism of fluorescence of the complex by CT-DNA is static quenching. The thermodynamic parameters ( and ) showed that main interaction with hydrogenic forces occurred that is intercalation mode. Also, CV results confirm this mode because, with increasing the CT-DNA concentration, shift to higher potential was observed.">
DNA相互作用的研究一个新的包含不同铂(II)复杂芳香二氮配体</gydF4y2Batitle>
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<strong>研究文章|<gydF4y2Ba!-- -->开放获取</gydF4y2Bastrong>
<div class="articleHeader__meta">
<span>体积<gydF4y2Ba!-- -->2011年<gydF4y2Ba!-- -->|</gydF4y2Baspan>
<span>文章的ID<gydF4y2Ba!-- -->429241年<gydF4y2Ba!-- -->|</gydF4y2Baspan>
<span class="articleHeader__meta_doiLink"><a href="https://doi.org/10.1155/2011/429241" aria-label="Doi-link" target="_blank" rel="noreferrer">https://doi.org/10.1155/2011/429241</gydF4y2Baa></span>
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<h1 class="articleHeader__title">DNA相互作用的研究一个新的包含不同铂(II)复杂芳香二氮配体</gydF4y2Bah1>
<div class="articleHeader__authors">
<span class="articleHeader__authors_author"><strong>Nahid Shahabadi</gydF4y2Bastrong><a href="//www.newsama.com/cdn-cgi/l/email-protection" aria-label="Mail Option"><span role="img" class="anticon">
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</svg></span></a>,<gydF4y2Basup>1</gydF4y2Basup></span>
<span class="articleHeader__authors_author">Somaye穆罕默<gydF4y2Ba!-- -->,<gydF4y2Basup>1</gydF4y2Basup></span>
<span class="articleHeader__authors_author">和<gydF4y2Ba!-- -->Robabeh Alizadeh<gydF4y2Basup>2</gydF4y2Basup></span>
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<strong>学术编辑器:</gydF4y2Bastrong>
<span>Zhe-Sheng陈</gydF4y2Baspan>
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<div class="articleHeader__timeline">
<div class="articleHeader__timeline_item ">
<strong>收到了</gydF4y2Bastrong>
<span>2011年7月20日</gydF4y2Baspan>
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<strong>修改后的</gydF4y2Bastrong>
<span>2011年9月12日</gydF4y2Baspan>
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<div class="articleHeader__timeline_item ">
<strong>接受</gydF4y2Bastrong>
<span>2011年9月13日</gydF4y2Baspan>
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<div class="articleHeader__timeline_item articleHeader__timeline_item_sticky">
<strong>发表</gydF4y2Bastrong>
<span>2011年12月14日</gydF4y2Baspan>
</div>
</div>
</div>
<div class="articleBody">
<div class="xml-content">
<h4 class="header" id="abstract">文摘</gydF4y2Bah4>
<p>一个新的单核Pt (II)复杂,[葡文(DMP) (DIP)] Cl<gydF4y2Basub>2</gydF4y2Basub>。H<gydF4y2Basub>2</gydF4y2Basub>O, DMP是4,4-dimethyl-2 2-bipyridine下降是4,7-diphenyl-1 10-phenantroline,已经合成,特点是物理化学和光谱方法。这个复杂的绑定交互与小牛胸腺DNA (CT-DNA)研究使用荧光法,分光光度法、圆二色性、粘度测量法和循环voltametry(简历)。紫外可见光谱显示4海里的吸收带红移280海里一起重大减色性吸收带的复杂。intrnisic绑定常量<gydF4y2Basvg height="18.012501" id="M1" style="vertical-align:-3.2316pt;width:124.1625px;" version="1.1" viewbox="0 0 124.1625 18.012501" width="124.1625" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,18.0125)">
<g transform="translate(72,-57.59)">
<text transform="matrix(1,0,0,-1,-71.95,60.86)">
<tspan style="font-size: 12.50px; " x="0" y="0">
(</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="4.1634989" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-58.26,57.74)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-50.17,60.86)">
<tspan style="font-size: 12.50px; " x="0" y="0">
=</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="12.027886" y="0">
2</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="21.055052" y="0">
×</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="31.832638" y="0">
1</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="38.084137" y="0">
0</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-5.82,66.03)">
<tspan style="font-size: 8.75px; " x="0" y="0">
4</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,1.13,60.86)">
<tspan style="font-size: 12.50px; " x="0" y="0">
米</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,12.25,65.87)">
<tspan style="font-size: 8.75px; " x="0" y="0">
−</gydF4y2Batspan>
<tspan style="font-size: 8.75px; " x="5.99512" y="0">
1</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,23.12,60.86)">
<tspan style="font-size: 12.50px; " x="0" y="0">
)</gydF4y2Batspan>
</text>
</g>
</g>
</svg>更符合intercalators并建议这个绑定模式。粘度的测量表明,complex-DNA交互可以疏水和确认夹层。此外,复杂的感应探测CT-DNA CD谱的变化。荧光的研究显示,可能的荧光猝灭机理复杂的CT-DNA是静态猝灭。热力学参数(<gydF4y2Basvg height="11.0625" id="M2" style="vertical-align:-0.30096pt;width:52.25px;" version="1.1" viewbox="0 0 52.25 11.0625" width="52.25" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="23.405617" y="0">
></gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="35.446007" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="11.0625" id="M3" style="vertical-align:-0.30096pt;width:48.775002px;" version="1.1" viewbox="0 0 48.775002 11.0625" width="48.775002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="20.617447" y="0">
></gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="32.657837" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>)表明,主要与水成的部队发生互动是夹层模式。同时,简历结果证实这种模式,因为随着CT-DNA浓度增加,转向更高的潜在的观察。</gydF4y2Bap>
<span class="end-abs"></span>
<h4 id="introduction">1。介绍</gydF4y2Bah4>
<p>在最近几年,只有一些铂化合物如顺铂、卡铂,和最近nedaplatin和铂一直被称为抗肿瘤化合物在临床治疗;为这一领域的研究仍在增长(<gydF4y2Baa href="#B1">1</gydF4y2Baa>- - - - - -<gydF4y2Baa href="#B5">5</gydF4y2Baa>]。metallointercalators最初的研究集中在广场平面含邻二氮杂菲铂(II)配合物配体(<gydF4y2Baa href="#B6a">6</gydF4y2Baa>,<gydF4y2Baa href="#B6b">7</gydF4y2Baa>]。包含三个关于环或邻二氮杂菲八面体过渡金属配合物配体已经被证明是槽绑定或可能部分intercalators [<gydF4y2Baa href="#B7">8</gydF4y2Baa>,<gydF4y2Baa href="#B8">9</gydF4y2Baa>]。关于过渡金属配合物与DNA之间的共价相互作用,他们可以通过夹层发生,槽绑定,或外部静电绑定(<gydF4y2Baa href="#B9">10</gydF4y2Baa>]。一般来说,金属配合物在绑定到DNA稳定通过一系列的弱相互作用等<gydF4y2Bai>π</gydF4y2Bai>堆积相互作用之间的芳香杂环组碱基对(夹层),氢键和范德华相互作用的官能团结合沿着DNA螺旋槽(<gydF4y2Baa href="#B10">11</gydF4y2Baa>]。铂化合物的一个重要组成部分的第一行治疗许多类型的癌症,如睾丸和卵巢癌症。顺铂、顺式[竞购<gydF4y2Basub>2</gydF4y2Basub>(NH<gydF4y2Basub>3</gydF4y2Basub>)<gydF4y2Basub>2</gydF4y2Basub>),卡铂,Pt (NH (cbca)<gydF4y2Basub>3</gydF4y2Basub>)<gydF4y2Basub>2</gydF4y2Basub>cbca = 1, 1-cyclobutanedicarboxylate,临床上已经使用超过30年。最近,铂,Pt(达奇)(牛),达奇= R, R 1, 2-diaminocycloexane和牛=草酸,第三代Pt复合物被批准用于临床使用的<gydF4y2Baa href="#B11">12</gydF4y2Baa>]。McFadyen et al。<gydF4y2Baa href="#B12">13</gydF4y2Baa>]合成了一系列含有取代邻二氮杂菲的Pt (II)配合物配体和显示复杂[葡文(en) (3、4、7, 8-Me<gydF4y2Basub>4</gydF4y2Basub>苯酚的)]Cl<gydF4y2Basub>2</gydF4y2Basub>比相应的两到三倍活跃phen-based复杂[葡文(en)(苯酚的)]Cl吗<gydF4y2Basub>2</gydF4y2Basub>对小鼠白血病细胞系。在这种背景下,我们报告的调查结果与小牛胸腺DNA的复杂互动[葡文(DMP) (DIP)] Cl<gydF4y2Basub>2</gydF4y2Basub>·H<gydF4y2Basub>2</gydF4y2Basub>o .的数据进一步与以前的结果(<gydF4y2Baa href="#B13">14</gydF4y2Baa>)来确定金属DNA结合模式的影响。许多技术已经申请调查金属配合物与DNA的相互作用。这些包括紫外分光光度法(i)等分子光谱方法(<gydF4y2Baa href="#B14">15</gydF4y2Baa>),荧光<gydF4y2Baa href="#B15">16</gydF4y2Baa>),圆二色性光谱(<gydF4y2Baa href="#B16">17</gydF4y2Baa>,<gydF4y2Baa href="#B17">18</gydF4y2Baa>),动态粘度测量(<gydF4y2Baa href="#B18">19</gydF4y2Baa>,<gydF4y2Baa href="#B19">20.</gydF4y2Baa>)和高效液相色谱法(<gydF4y2Baa href="#B20">21</gydF4y2Baa>]。之间的这些技术,紫外分光光度法、荧光法、动态粘度测量圆二色性和循环voltametry研究的首选,因为小分子DNA相互作用可能是实验进行光谱峰的强度和位置的变化反应或DNA的动态粘度的变化。</gydF4y2Bap>
<h4 id="experimental">2。实验</gydF4y2Bah4>
<p>所有的化学物质如K<gydF4y2Basub>2</gydF4y2Basub>竞购<gydF4y2Basub>4</gydF4y2Basub>4 7-dimethyl-1 10-phenanthroline, 4, 4-dimethyl-2, 2-bypyridine,从默克公司购买,Tris-HCl和高度聚合的小牛胸腺DNA (CT-DNA)从σ公司购买. .实验在Tris-HCl缓冲区进行pH值7.0。小牛胸腺DNA的一个解决方案给的紫外吸光度比260和280 nm超过1.8,表明DNA从蛋白质有充分的自由<gydF4y2Baa href="#B21">22</gydF4y2Baa>]。CT-DNA准备的储备溶液溶解DNA在10毫米Tris-HCl缓冲区的pH值7.0。DNA浓度(单体)股票的解决方案(1×10<gydF4y2Basup>−2</gydF4y2Basup>每个核苷酸M)是由紫外分光光度计,在适当稀释样品,使用摩尔吸收系数6600米<gydF4y2Basup>−1</gydF4y2Basup>厘米<gydF4y2Basup>−1</gydF4y2Basup>在258纳米<gydF4y2Baa href="#B22">23</gydF4y2Baa>]。股票的解决方案被储存在4°C和使用不超过4天。</gydF4y2Bap>
<h5 id="sec2.1">2.1。合成的[葡文(DMP) (DIP)] Cl<gydF4y2Basub>2</gydF4y2Basub>·H<gydF4y2Basub>2</gydF4y2Basub>复杂啊</gydF4y2Bah5>
<p>[Pt (DMP) (DIP)] Cl<gydF4y2Basub>2</gydF4y2Basub>·H<gydF4y2Basub>2</gydF4y2Basub>O(图<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig1/" target="_blank">1</gydF4y2Baa>)是合成了两个步骤。在第一步中铂前体,K<gydF4y2Basub>2</gydF4y2Basub>竞购<gydF4y2Basub>4</gydF4y2Basub>,(0.05克,0.12更易)溶解在6毫升的水和一个解决方案4,4-dimethyl-2, 2-bipyridine(0.022、0.12更易)是一滴一滴地补充道。混合搅拌过夜在50°C。成立一个黄色沉淀,过滤,用冰冷的水和丙酮。随后在第一步第二步准备的沉淀(0.03克,0.1更易)溶解在4毫升甲醇和解决方案4,7-diphenyl-1, 10-phenantroline(0.027克,0.1更易)4毫升甲醇添加一滴一滴地。最后的解决方案是在180°C回流4 h。成立一个绿色沉淀,过滤,用冰冷的水和二乙醚。收益率:36%。元素分析对PtC(列车自动控制系统)<gydF4y2Basub>36</gydF4y2Basub>H<gydF4y2Basub>28</gydF4y2Basub>N<gydF4y2Basub>4</gydF4y2Basub>Cl<gydF4y2Basub>2</gydF4y2Basub>H<gydF4y2Basub>2</gydF4y2Basub>O:发现:C, 53.2;H, 4.1;N, 6.9%;钙:C, 54.1;H, 3.6;N, 7.0%;傅立叶变换红外光谱(KBr,厘米<gydF4y2Basup>−1</gydF4y2Basup>):3050 - 3236 (<gydF4y2Bai><i>ν</gydF4y2Bai></i>(碳氢键),1230 (<gydF4y2Bai><i>ν</gydF4y2Bai></i>(氮),1560 (<gydF4y2Bai><i>ν</gydF4y2Bai></i>532 (C = N)),<gydF4y2Bai><i>ν</gydF4y2Bai></i>(Pt-N)], 3400 - 3500 (<gydF4y2Bai>ν</gydF4y2Bai>(H<gydF4y2Basub>2</gydF4y2Basub>O)]。</gydF4y2Bap>
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<span class="caption-text">图1</gydF4y2Baspan>
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<td>[葡文的结构(DMP) (DIP)] Cl<gydF4y2Basub>2</gydF4y2Basub>·H<gydF4y2Basub>2</gydF4y2Basub>O复杂。</gydF4y2Batd>
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<h5 id="sec2.2">2.2。仪表</gydF4y2Bah5>
<p><sup>1</gydF4y2Basup>H NMR光谱被记录使用力量皇冠DPX200 MHz (4.7 T)光谱仪使用d6-DMSO作为溶剂。元素分析是使用贺利氏链执行元素分析仪。吸光度光谱记录使用惠普分光光度计(安捷伦8453)配有恒温浴(Huberpolysat cc1)。吸收滴定实验由DNA的浓度保持恒定(5×10<gydF4y2Basup>−5</gydF4y2Basup>米),而不同的复杂的浓度5×10<gydF4y2Basup>−5</gydF4y2Basup>4×10 M<gydF4y2Basup>−4</gydF4y2Basup>米(ri = (DNA) /(复杂的)= 0 - 4)。</gydF4y2Bap>
<p>吸光度值记录在每个连续的DNA溶液和平衡(ca。10分钟)(图<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig2/" target="_blank">2</gydF4y2Baa>)。数据被安装(<gydF4y2Baa href="#EEq1">1</gydF4y2Baa>)获得内在约束力的常数,<gydF4y2Basvg height="14.3625" id="M4" style="vertical-align:-3.2316pt;width:17.799999px;" version="1.1" viewbox="0 0 17.799999 14.3625" width="17.799999" xmlns="http://www.w3.org/2000/svg">
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</g>
</g>
</svg>被孤立的校准曲线确定金属络合物水溶液;比尔定律后,<gydF4y2Basvg height="11.0375" id="M9" style="vertical-align:-3.22282pt;width:13.8px;" version="1.1" viewbox="0 0 13.8 11.0375" width="13.8" xmlns="http://www.w3.org/2000/svg">
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</gydF4y2Batspan>
</text>
</g>
</g>
</svg>确定测量吸光度之间的比例和Pt (II)复杂的浓度,<gydF4y2Bai>一个</gydF4y2Bai>奥林匹克广播服务公司/[葡文]。一块(DNA) /<gydF4y2Basvg height="16.637501" id="M10" style="vertical-align:-4.77652pt;width:57.4375px;" version="1.1" viewbox="0 0 57.4375 16.637501" width="57.4375" xmlns="http://www.w3.org/2000/svg">
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<text transform="matrix(1,0,0,-1,-30.26,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
)</gydF4y2Batspan>
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</g>
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</svg>与(DNA)给斜率为1 / (<gydF4y2Basvg height="12.975" id="M11" style="vertical-align:-4.77652pt;width:46.674999px;" version="1.1" viewbox="0 0 46.674999 12.975" width="46.674999" xmlns="http://www.w3.org/2000/svg">
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<g transform="translate(72,-61.62)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
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<text transform="matrix(1,0,0,-1,-65.9,63.31)">
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<text transform="matrix(1,0,0,-1,-58.51,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
−</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="11.340221" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-41.11,63.31)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
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</svg>)和一个<gydF4y2Bai>Y</gydF4y2Bai>拦截等于1 /<gydF4y2Basvg height="14.3625" id="M12" style="vertical-align:-3.2316pt;width:17.799999px;" version="1.1" viewbox="0 0 17.799999 14.3625" width="17.799999" xmlns="http://www.w3.org/2000/svg">
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</svg>(<gydF4y2Basvg height="12.975" id="M13" style="vertical-align:-4.77652pt;width:46.674999px;" version="1.1" viewbox="0 0 46.674999 12.975" width="46.674999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,12.975)">
<g transform="translate(72,-61.62)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
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<text transform="matrix(1,0,0,-1,-65.9,63.31)">
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<text transform="matrix(1,0,0,-1,-58.51,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
−</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="11.340221" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-41.11,63.31)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
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</g>
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</svg>);<gydF4y2Basvg height="14.3625" id="M14" style="vertical-align:-3.2316pt;width:17.799999px;" version="1.1" viewbox="0 0 17.799999 14.3625" width="17.799999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3625)">
<g transform="translate(72,-60.51)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>斜率的比值是吗<gydF4y2Basvg height="10.325" id="M15" style="vertical-align:-0.0pt;width:11.6125px;" version="1.1" viewbox="0 0 11.6125 10.325" width="11.6125" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>拦截。CD测量记录在JASCO (j - 810)分光偏振计,DNA的浓度保持恒定(5×10<gydF4y2Basup>−5</gydF4y2Basup>米)在不同复杂的浓度(ri =(复杂的)/ (DNA) = ri = 0, 0.4, 0.5)。</gydF4y2Bap>
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<span class="caption-text">图2</gydF4y2Baspan>
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<td>吸收光谱的DNA (5×10<gydF4y2Basup>−5</gydF4y2Basup>米)在没有和铂存在越来越多的复杂(ri = (DNA) /(复杂的))。</gydF4y2Batd>
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</tbody>
</table>
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<p>粘度测量是使用粘度计(散粒AVS 450)维持在25.0±0.5°C使用恒温浴。DNA的浓度是固定在5×10<gydF4y2Basup>−5</gydF4y2Basup>M,流时间和数字秒表测量。三个测量值的平均值被用来评估粘度<gydF4y2Bai>η</gydF4y2Bai>的样本。的值相对比粘度(<gydF4y2Basvg height="9.875" id="M16" style="vertical-align:-2.29482pt;width:7.9375px;" version="1.1" viewbox="0 0 7.9375 9.875" width="7.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.875)">
<g transform="translate(72,-64.1)">
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</gydF4y2Batspan>
</text>
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</svg>/<gydF4y2Basvg height="11.075" id="M17" style="vertical-align:-3.25793pt;width:14.0375px;" version="1.1" viewbox="0 0 14.0375 11.075" width="14.0375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.075)">
<g transform="translate(72,-63.14)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-65.7,63.31)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>)<gydF4y2Basup>1/3</gydF4y2Basup>,在那里<gydF4y2Basvg height="11.075" id="M18" style="vertical-align:-3.25793pt;width:14.0375px;" version="1.1" viewbox="0 0 14.0375 11.075" width="14.0375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.075)">
<g transform="translate(72,-63.14)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
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</gydF4y2Batspan>
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<text transform="matrix(1,0,0,-1,-65.7,63.31)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="9.875" id="M19" style="vertical-align:-2.29482pt;width:7.9375px;" version="1.1" viewbox="0 0 7.9375 9.875" width="7.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.875)">
<g transform="translate(72,-64.1)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>是DNA的特定粘度的贡献没有(<gydF4y2Basvg height="11.075" id="M20" style="vertical-align:-3.25793pt;width:14.0375px;" version="1.1" viewbox="0 0 14.0375 11.075" width="14.0375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.075)">
<g transform="translate(72,-63.14)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-65.7,63.31)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>在复杂的存在)和(<gydF4y2Basvg height="9.875" id="M21" style="vertical-align:-2.29482pt;width:7.9375px;" version="1.1" viewbox="0 0 7.9375 9.875" width="7.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.875)">
<g transform="translate(72,-64.1)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>),策划反对ri (ri =(复杂的)/ (DNA) = 0.0 - -2.5)。</gydF4y2Bap>
<p>所有荧光测量进行了JASCO荧光谱仪(FP6200)通过保持复杂的浓度不变而改变DNA浓度从0到36×10<gydF4y2Basup>−5</gydF4y2Basup>米(ri = (DNA) /(复杂的)= 0.0,0.5,1.0,1.5,3、3.5)在三种不同温度(288、298、308 K)。</gydF4y2Bap>
<p>循环伏安测量进行了使用一个AUTOLAB模型(PG STAT C),与三电极系统:0.10厘米直径玻璃碳(GC)盘作为工作电极,Ag / AgCl电极作为参比电极,和Pt线作为对电极。电化学实验进行了室温在25 ml伏安细胞。所有的潜力被称为Ag / AgCl参考。表面与0.05毫米新鲜抛光氧化铝每个实验之前,使用每个抛光步骤之间重蒸馏的水冲洗。支持电解质为0.01 M的Tris-HCl缓冲溶液制备(pH值7.4)重蒸馏的水。在实验之前,解决方案是通过清除真空用纯氮气1分钟,期间,通过测量氮流解决方案。电位曲线和实验数据都记录在软件gp (<gydF4y2Baa href="#B24">25</gydF4y2Baa>]。</gydF4y2Bap>
<h4 id="results-and-discussion">3所示。结果与讨论</gydF4y2Bah4>
<h5 id="sec3.1">3.1。合成和表征[葡文(DMP) (DIP)] Cl<gydF4y2Basub>2</gydF4y2Basub>·H<gydF4y2Basub>2</gydF4y2Basub>复杂啊</gydF4y2Bah5>
<p>混合配体铂(II)的复杂4-dimethyl-2, 2-bipyridine (DMP)和4,7-diphenyl-1, 10-phenantroline (DIP)在甲醇水溶液合成使用K<gydF4y2Basub>2</gydF4y2Basub>竞购<gydF4y2Basub>4</gydF4y2Basub>。复杂的符合公式[葡文(DMP) (DIP)] Cl<gydF4y2Basub>2</gydF4y2Basub>·H<gydF4y2Basub>2</gydF4y2Basub>O,元素分析的基础上确定。</gydF4y2Bap>
<p>复杂的红外光谱特征是一个乐队的出现在532厘米<gydF4y2Basup>−1</gydF4y2Basup>由于[<gydF4y2Bai>ν</gydF4y2Bai>(Pt-N)]。协调与确诊的氮原子是这个乐队的存在。然而,宽带在3400 - 3500厘米<gydF4y2Basup>−1</gydF4y2Basup>是分配给<gydF4y2Bai>ν</gydF4y2Bai>(H<gydF4y2Basub>2</gydF4y2Basub>O)]。其他的乐队在3050 - 3236 (<gydF4y2Bai><i>ν</gydF4y2Bai></i>(碳氢键),1230 (<gydF4y2Bai><i>ν</gydF4y2Bai></i>(氮),1560 (<gydF4y2Bai><i>ν</gydF4y2Bai></i>分别(C = N)]。</gydF4y2Bap>
<p><span class="statement" id="head1"><span class="statement-title"><i class="statement-title"><sup>1</gydF4y2Basup>H NMR (d6-DMSO)</gydF4y2Bai></span><br>芳族地区的信号<gydF4y2Bai>δ</gydF4y2Bai>= 8.3,<gydF4y2Bai>δ</gydF4y2Bai>= 8.1,<gydF4y2Bai>δ</gydF4y2Bai>= 7.1 ppm被分配到质子DMP配体和信号<gydF4y2Bai>δ</gydF4y2Bai>= 8.9,<gydF4y2Bai>δ</gydF4y2Bai>= 7.6,<gydF4y2Bai>δ</gydF4y2Bai>= 7.5,<gydF4y2Bai>δ</gydF4y2Bai>= 7.3的质子被分配到配体。DMP和浸渍配体的质子转移(<gydF4y2Bai>≈</gydF4y2Bai>−0.2 ppm)与相应的自由配体和络合。</gydF4y2Baspan></p>
<h5 id="sec3.2">3.2。紫外可见光谱</gydF4y2Bah5>
<p>吸收光谱是一种最有用的技术来研究DNA的绑定任何药物。减色性的程度通常表明intercalative粘结强度(<gydF4y2Baa href="#B25">26</gydF4y2Baa>]。夹层的减色性特征通常被归因于电子态之间的相互作用的化合物与DNA碱基(<gydF4y2Baa href="#B26">27</gydF4y2Baa>),而红移与能源的减少有关差距HOMO和LUMO绑定后复杂的DNA分子轨道(<gydF4y2Baa href="#B27">28</gydF4y2Baa>]。Hyperchromism已经观察了许多药物与DNA的相互作用[<gydF4y2Baa href="#B28">29日</gydF4y2Baa>]。增色效应可能归因于外部联系(静电绑定(<gydF4y2Baa href="#B29">30.</gydF4y2Baa>])或开卷部分DNA的螺旋结构,揭露更多基地的DNA (<gydF4y2Baa href="#B30">31日</gydF4y2Baa>]。我们发现4海里的吸收带红移280海里一起重大减色性吸收带的复杂。这个观察提供了很好的证据的夹层Pt(2)复杂的叠加和交互的芳香环配体和DNA碱基对<gydF4y2Baa href="#B31">32</gydF4y2Baa>]。此外,基于光谱滴定法,获得的吸光度值的[葡文(DMP) (DIP)] Cl<gydF4y2Basub>2</gydF4y2Basub>·H<gydF4y2Basub>2</gydF4y2Basub>O complex-DNA绑定常量,<gydF4y2Basvg height="14.3625" id="M22" style="vertical-align:-3.2316pt;width:17.799999px;" version="1.1" viewbox="0 0 17.799999 14.3625" width="17.799999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3625)">
<g transform="translate(72,-60.51)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
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</svg>,计算中所描述的实验过程。获得的价值<gydF4y2Basvg height="14.3625" id="M23" style="vertical-align:-3.2316pt;width:17.799999px;" version="1.1" viewbox="0 0 17.799999 14.3625" width="17.799999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3625)">
<g transform="translate(72,-60.51)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
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</svg>为2.0±0.2×10<gydF4y2Basup>4</gydF4y2Basup>米<gydF4y2Basup>−1</gydF4y2Basup>。的值<gydF4y2Basvg height="14.3625" id="M24" style="vertical-align:-3.2316pt;width:17.799999px;" version="1.1" viewbox="0 0 17.799999 14.3625" width="17.799999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3625)">
<g transform="translate(72,-60.51)">
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</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
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</gydF4y2Batspan>
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</svg>中描述的文学经典intercalators (ethidium-DNA<gydF4y2Basvg height="14.225" id="M25" style="vertical-align:-0.3135pt;width:46.612499px;" version="1.1" viewbox="0 0 46.612499 14.225" width="46.612499" xmlns="http://www.w3.org/2000/svg">
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7</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
×</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="19.804752" y="0">
1</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="26.056252" y="0">
0</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-39.64,66.16)">
<tspan style="font-size: 8.75px; " x="0" y="0">
7</gydF4y2Batspan>
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</svg>米<gydF4y2Basup>−1</gydF4y2Basup>(<gydF4y2Baa href="#B32">33</gydF4y2Baa>)和proflavin-DNA 4.1×10<gydF4y2Basup>5</gydF4y2Basup>米<gydF4y2Basup>−1</gydF4y2Basup>(<gydF4y2Baa href="#B33">34</gydF4y2Baa>])至少30或20数量级高于获得Pt(2)复杂。这个结果表明,碱基对之间的夹层不是主要的交互方式Pt (II)复杂的DNA。相比之下,的价值<gydF4y2Basvg height="14.3625" id="M26" style="vertical-align:-3.2316pt;width:17.799999px;" version="1.1" viewbox="0 0 17.799999 14.3625" width="17.799999" xmlns="http://www.w3.org/2000/svg">
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</svg>是十个数量级高于<gydF4y2Basvg height="14.3625" id="M27" style="vertical-align:-3.2316pt;width:17.799999px;" version="1.1" viewbox="0 0 17.799999 14.3625" width="17.799999" xmlns="http://www.w3.org/2000/svg">
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</svg>值被发现的化合物与槽绑定的方式像Cr (II)配合物<gydF4y2Baa href="#B34">35</gydF4y2Baa>10-phen]或三(1)钌(II) DNA (<gydF4y2Baa href="#B35">36</gydF4y2Baa>]。这些结果证实,Pt (II)复杂强烈与DNA的相互作用,以及<gydF4y2Basvg height="14.3625" id="M28" style="vertical-align:-3.2316pt;width:17.799999px;" version="1.1" viewbox="0 0 17.799999 14.3625" width="17.799999" xmlns="http://www.w3.org/2000/svg">
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</svg>价值获得了这个复杂的相同的数量级,确定ZnL类似条件<gydF4y2Basup>+ 2</gydF4y2Basup>(<gydF4y2Basvg height="18.012501" id="M29" style="vertical-align:-3.2316pt;width:148.21249px;" version="1.1" viewbox="0 0 148.21249 18.012501" width="148.21249" xmlns="http://www.w3.org/2000/svg">
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=</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="12.027886" y="0">
7</gydF4y2Batspan>
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。</gydF4y2Batspan>
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3</gydF4y2Batspan>
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6</gydF4y2Batspan>
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±</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="48.036526" y="0">
0</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="54.288025" y="0">
。</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="57.413776" y="0">
0</gydF4y2Batspan>
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1</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="72.692444" y="0">
×</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="83.470032" y="0">
1</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="89.721527" y="0">
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4</gydF4y2Batspan>
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</svg>米<gydF4y2Basup>−1</gydF4y2Basup>)置入视为一个复杂的<gydF4y2Baa href="#B36">37</gydF4y2Baa>]。</gydF4y2Bap>
<h6 id="sec3.2.1">3.2.1之上。圆二色性光谱</gydF4y2Bah6>
<p>建立详细是否绑定的复杂带来任何明显的DNA双螺旋结构的构象变化,圆二色性(CD)光谱CT-DNA记录增加复杂/ CT-DNA比率。自然小牛胸腺DNA的观察到CD谱由一个积极的乐队在275海里由于基地叠加和消极的乐队在245 nm由于螺旋性,这是DNA在右撇子B形式(图的特征<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig3/" target="_blank">3</gydF4y2Baa>)[<gydF4y2Baa href="#B37">38</gydF4y2Baa>]。复杂的构象的影响DNA的二级结构研究了CT-DNA的浓度保持在5×10<gydF4y2Basup>−5</gydF4y2Basup>米而不同复杂的缓冲溶液的浓度10毫米的三(ri = 0、0.4、0.5)。的频谱CT-DNA和添加剂的监控从215到800纳米。如图<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig3/" target="_blank">3</gydF4y2Baa>DNA的CD谱展览积极吸收282海里由于基地叠加和消极的乐队在240 nm由于B-DNA的螺旋性。在铂(II)复杂,正峰的强度增加,负峰的强度降低。CD光谱的变化复杂的存在显示稳定的右手B CT-DNA形式(图<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig3/" target="_blank">3</gydF4y2Baa>)。</gydF4y2Bap>
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<span class="caption-text">图3</gydF4y2Baspan>
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<td>圆二色性光谱CT-DNA (5×10<gydF4y2Basup>−5</gydF4y2Basup>米)的存在越来越多的复杂Pt (II)在下列化学计量比(ri =(复杂的)/ (DNA) = 0.0, 0.4, 0.5)。</gydF4y2Batd>
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<h6 id="sec3.2.2">3.2.2。粘度测量</gydF4y2Bah6>
<p>粘度测量CT-DNA被视为最模糊的绑定模型的和最关键的测试解决方案没有晶体结构的数据。的值相对比粘度(<gydF4y2Basvg height="9.875" id="M30" style="vertical-align:-2.29482pt;width:7.9375px;" version="1.1" viewbox="0 0 7.9375 9.875" width="7.9375" xmlns="http://www.w3.org/2000/svg">
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</svg>)<gydF4y2Basup>1/3</gydF4y2Basup>(<gydF4y2Basvg height="11.075" id="M32" style="vertical-align:-3.25793pt;width:14.0375px;" version="1.1" viewbox="0 0 14.0375 11.075" width="14.0375" xmlns="http://www.w3.org/2000/svg">
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0</gydF4y2Batspan>
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</svg>DNA的特定粘度的贡献是在没有和Pt的存在复杂,职责)策划反对1 /<gydF4y2Bai>R</gydF4y2Bai>(<gydF4y2Bai>R</gydF4y2Bai>= (DNA) /(复杂的)(图)<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig4/" target="_blank">4</gydF4y2Baa>)。众所周知,槽粘合剂像赫斯特33258年不会引起的轴向长度增加DNA (<gydF4y2Baa href="#B38">39</gydF4y2Baa>,<gydF4y2Baa href="#B39">40</gydF4y2Baa>),因此没有改变的相对粘度。相比之下,顺铂是已知DNA通过共价链接绑定,缩短轴向长度的双螺旋结构<gydF4y2Baa href="#B40">41</gydF4y2Baa>),导致溶液的相对粘度下降。也部分intercalators减少轴向长度观察相对粘度降低,而经典的有机intercalators如溴化乙锭的轴向长度增加DNA,和它变得更加刚性<gydF4y2Baa href="#B38">39</gydF4y2Baa>,<gydF4y2Baa href="#B39">40</gydF4y2Baa>)导致的相对粘度的增加。结果证实粘度测量灵敏度的DNA结合的不同模式。在这项研究中,观察到,提高铂复杂铅浓度DNA的粘度增加。因此,我们可以推断出[葡文(DMP) (DIP)] Cl<gydF4y2Basub>2</gydF4y2Basub>·H<gydF4y2Basub>2</gydF4y2Basub>O intercalator复杂当然是DNA。自交互铂(II)与DNA可以让DNA不再复杂,我们可以期待DNA相对粘度的增加,边坡在0到0.94之间(测量值为溴化乙锭(<gydF4y2Baa href="#B41">42</gydF4y2Baa>)如果Pt (II)的夹层复杂要么只有一个交互模式或强于其他交互(s)(图<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig6/" target="_blank">6</gydF4y2Baa>)。但是,在这项研究中,DNA的相对粘度增加斜率为0.38(图<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig4/" target="_blank">4</gydF4y2Baa>)和合理的认为,可能是其他DNA之间的相互作用(s)和工党(II)复杂的发生,并为斜率的减少是合理的。此外,需要注意的是,这个Pt的DNA结合常数测量(2)复杂的溴化乙锭是低于决定。因此,增加粘度越大观察溴化乙锭Pt (II)相比,复杂的可能是由于降低后者的结合常数的DNA。这些结果清楚地表明使用一些技术来确定夹层的重要性。</gydF4y2Bap>
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<span class="caption-text">图4</gydF4y2Baspan>
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<td>越来越多的Pt的影响(2)复杂的粘度CT-DNA (5×10<gydF4y2Basup>−5</gydF4y2Basup>米)在10毫米Tris-HCl缓冲区。</gydF4y2Batd>
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<h6 id="sec3.2.3">3.2.3。荧光研究</gydF4y2Bah6>
<p>进一步研究复杂和CT-DNA之间的互动模式,荧光滴定实验进行。复杂的发射发光Tris-HCl缓冲区的最大波长约为386纳米。图<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig5/" target="_blank">5</gydF4y2Baa>显示了复杂的发射光谱在没有和不同数量的CT-DNA的存在。在复杂的发射光谱,增加CT-DNA浓度排放强度下降是由于self-stacking一些免费的基地在沿着DNA复合表面(<gydF4y2Baa href="#B42">43</gydF4y2Baa>]。淬火可以通过不同的机制来实现,这通常分为动态和静态猝灭。动态猝灭是指一个过程的荧光团和饮料接触在瞬态存在的退出状态,而静态猝灭是指fluorophore-quencher复杂的形成。一般来说,动态和静态淬火可以区分他们的不同温度和激发态寿命的依赖。因为在这两种情况下,荧光强度与浓度的饮料,猝灭荧光团可以作为一个指标的淬火剂(<gydF4y2Baa href="#B43">44</gydF4y2Baa>]。</gydF4y2Bap>
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<span class="caption-text">图5</gydF4y2Baspan>
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<td>荧光光谱的Pt(2)复杂的几个CT-DNA浓度。</gydF4y2Batd>
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<span class="caption-text">图6</gydF4y2Baspan>
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<td>范霍夫情节Pt (II)交互的复杂和CT-DNA pH值7.2。</gydF4y2Batd>
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<p>荧光猝灭被Stern-Volmer方程描述:<gydF4y2Baspan class="equation" id="EEq2">
<svg height="32.849998" id="M34" preserveaspectratio="xMaxYMin" style="vertical-align:-0pt;width:700.9375px;" version="1.1" viewbox="0 0 700.9375 32.849998" width="700.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,32.85)">
<g transform="translate(72,-45.72)">
<text transform="matrix(1,0,0,-1,120.06,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,127.79,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
<g>
<path d="m 120.06,57.65 12.6,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path>
</g>
<text transform="matrix(1,0,0,-1,121.59,45.77)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="15.74" y="-8.75">
=</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="27.767885" y="-8.75">
1</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="36.807556" y="-8.75">
+</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="48.147778" y="-8.75">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,179.27,51.4)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,184.13,54.52)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,190.53,51.4)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,197.49,54.49)">
<tspan style="font-size: 12.50px; " x="0" y="0">
(</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="4.1599998" y="-0.029999999">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="13.93" y="0">
]</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="21.559999" y="-0.029999999">
=</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="33.587887" y="-0.029999999">
1</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="42.627556" y="-0.029999999">
+</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="53.967777" y="-0.029999999">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,260.99,51.4)">
<tspan style="font-size: 8.75px; " x="0" y="0">
年代</gydF4y2Batspan>
<tspan style="font-size: 8.75px; " x="4.6998239" y="0">
V</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,274.58,54.49)">
<tspan style="font-size: 12.50px; " x="0" y="0">
(</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="4.1700001" y="-0.029999999">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="13.93" y="0">
]</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="20.18" y="-0.029999999">
,</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="199.53999" y="-0.029999999">
(</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="203.70351" y="-0.029999999">
2</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="209.955" y="-0.029999999">
)</gydF4y2Batspan>
</text>
</g>
</g>
</svg></span>在哪里<gydF4y2Basvg height="14.3875" id="M35" style="vertical-align:-3.25793pt;width:15.875px;" version="1.1" viewbox="0 0 15.875 14.3875" width="15.875" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3875)">
<g transform="translate(72,-60.49)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-64.22,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="10.325" id="M36" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>代表了荧光强度没有在冷却器的存在,分别。<gydF4y2Basvg height="16.237499" id="M37" style="vertical-align:-4.74141pt;width:18.1px;" version="1.1" viewbox="0 0 18.1 16.237499" width="18.1" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,16.2375)">
<g transform="translate(72,-59.01)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>是荧光团淬火速率常数,<gydF4y2Basvg height="14.3875" id="M38" style="vertical-align:-3.25792pt;width:26.424999px;" version="1.1" viewbox="0 0 26.424999 14.3875" width="26.424999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3875)">
<g transform="translate(72,-60.49)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
年代</gydF4y2Batspan>
<tspan style="font-size: 8.75px; " x="4.691072" y="0">
V</gydF4y2Batspan>
</text>
</g>
</g>
</svg>是猝灭常数,<gydF4y2Basvg height="10.875" id="M39" style="vertical-align:-3.25793pt;width:14.225px;" version="1.1" viewbox="0 0 14.225 10.875" width="14.225" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.875)">
<g transform="translate(72,-63.3)">
<text transform="matrix(1,0,0,-1,-71.95,66.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-65.55,63.47)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>是荧光团的生命周期在缺乏冷却器(<gydF4y2Basvg height="10.875" id="M40" style="vertical-align:-3.25793pt;width:14.225px;" version="1.1" viewbox="0 0 14.225 10.875" width="14.225" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.875)">
<g transform="translate(72,-63.3)">
<text transform="matrix(1,0,0,-1,-71.95,66.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-65.55,63.47)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>= 10<gydF4y2Basup>−8</gydF4y2Basup>)和(<gydF4y2Basvg height="12.925" id="M41" style="vertical-align:-1.90608pt;width:12.325px;" version="1.1" viewbox="0 0 12.325 12.925" width="12.325" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,12.925)">
<g transform="translate(72,-61.66)">
<text transform="matrix(1,0,0,-1,-71.95,63.61)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>)是饮料的浓度(<gydF4y2Baa href="#B44">45</gydF4y2Baa>]。结果在表<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/tab1/" target="_blank">1</gydF4y2Baa>表明这个复杂的可能的猝灭机制CT-DNA涉及静态猝灭,因为<gydF4y2Basvg height="14.3875" id="M42" style="vertical-align:-3.25792pt;width:26.424999px;" version="1.1" viewbox="0 0 26.424999 14.3875" width="26.424999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3875)">
<g transform="translate(72,-60.49)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
年代</gydF4y2Batspan>
<tspan style="font-size: 8.75px; " x="4.691072" y="0">
V</gydF4y2Batspan>
</text>
</g>
</g>
</svg>随着温度增加而减小(<gydF4y2Baa href="#B45">46</gydF4y2Baa>]。</gydF4y2Bap>
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<span class="caption-text">表1</gydF4y2Baspan>
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<div class="floats-partial-caption-text">
猝灭常数CT-DNA Pt(2)复杂的在不同的温度下。</gydF4y2Badiv>
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<h5 id="sec3.3">3.3。结合常数和结合位点的数量</gydF4y2Bah5>
<p>如果假设有相似的和独立的生物分子的结合位点,结合常数(<gydF4y2Basvg height="16.2875" id="M47" style="vertical-align:-4.77652pt;width:20.0375px;" version="1.1" viewbox="0 0 20.0375 16.2875" width="20.0375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,16.2875)">
<g transform="translate(72,-58.97)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>)和结合位点的数量(<gydF4y2Basvg height="7.1374998" id="M48" style="vertical-align:-0.10033pt;width:7.8874998px;" version="1.1" viewbox="0 0 7.8874998 7.1374998" width="7.8874998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.1375)">
<g transform="translate(72,-66.29)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>)可以根据描述的方法取决于胡锦涛et al。(<gydF4y2Baa href="#B46">47</gydF4y2Baa>),使用下面的公式:<gydF4y2Baspan class="equation" id="EEq3">
<svg height="38.575001" id="M49" preserveaspectratio="xMaxYMin" style="vertical-align:-0pt;width:700.9375px;" version="1.1" viewbox="0 0 700.9375 38.575001" width="700.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,38.575)">
<g transform="translate(72,-41.14)">
<text transform="matrix(1,0,0,-1,107.75,53.45)">
<tspan style="font-size: 12.50px; " x="22.23" y="7.3299999">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="32.799999" y="-9.2600002">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="0" y="0">
l</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="7.6393328" y="0">
o</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="13.890833" y="0">
g</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,148.28,59.58)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,155.93,62.71)">
<tspan style="font-size: 12.50px; " x="0" y="0">
−</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="11.340221" y="0">
</gydF4y2Batspan>
</text>
<g>
<path d="m 140.55,56.57 36.26,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path>
</g>
<text transform="matrix(1,0,0,-1,153.91,44.69)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="24.1" y="-1.4299999">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="36.950001" y="-8.7600002">
=</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="48.977886" y="-8.7600002">
l</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="56.617218" y="-8.7600002">
o</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="62.868717" y="-8.7600002">
g</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="71.208221" y="-8.7600002">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,234.65,50.32)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,243.83,53.45)">
<tspan style="font-size: 12.50px; " x="41.869999" y="0.039999999">
(</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="46.029999" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="55.799999" y="0.039999999">
]</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="0" y="0">
+</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="11.340221" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="19.642214" y="0">
l</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="27.281546" y="0">
o</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="33.533047" y="0">
g</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="62.049999" y="0">
。</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="230.28999" y="0">
(</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="234.45351" y="0">
3</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="240.705" y="0">
)</gydF4y2Batspan>
</text>
</g>
</g>
</svg></span>在这里,<gydF4y2Basvg height="14.3875" id="M50" style="vertical-align:-3.25793pt;width:15.875px;" version="1.1" viewbox="0 0 15.875 14.3875" width="15.875" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3875)">
<g transform="translate(72,-60.49)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-64.22,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="10.325" id="M51" style="vertical-align:-0.0pt;width:12.05px;" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>荧光团的荧光强度在不同浓度的缺失和存在CT-DNA,分别。的值<gydF4y2Basvg height="16.2875" id="M52" style="vertical-align:-4.77652pt;width:20.0375px;" version="1.1" viewbox="0 0 20.0375 16.2875" width="20.0375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,16.2875)">
<g transform="translate(72,-58.97)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="7.1374998" id="M53" style="vertical-align:-0.10033pt;width:7.8874998px;" version="1.1" viewbox="0 0 7.8874998 7.1374998" width="7.8874998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.1375)">
<g transform="translate(72,-66.29)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>被发现是1.14×10吗<gydF4y2Basup>5</gydF4y2Basup>米<gydF4y2Basup>−1</gydF4y2Basup>分别和1(表<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/tab2/" target="_blank">2</gydF4y2Baa>)。</gydF4y2Bap>
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<div class="floats-partial-footer">
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<span class="caption-text">表2</gydF4y2Baspan>
<a aria-label="View full page" class="caption-partial-url" href="//www.newsama.com/journals/bca/2011/429241/tab2/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
绑定常量(<gydF4y2Basvg height="16.2875" id="M54" style="vertical-align:-4.77652pt;width:20.0375px;" version="1.1" viewbox="0 0 20.0375 16.2875" width="20.0375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,16.2875)">
<g transform="translate(72,-58.97)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
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<text transform="matrix(1,0,0,-1,-62.42,60.66)">
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</g>
</g>
</svg>)和结合位点的数量(<gydF4y2Basvg height="7.1374998" id="M55" style="vertical-align:-0.10033pt;width:7.8874998px;" version="1.1" viewbox="0 0 7.8874998 7.1374998" width="7.8874998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.1375)">
<g transform="translate(72,-66.29)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>)complex-DNA系统。</gydF4y2Badiv>
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</div>
</div>
<h6 id="sec3.3.1">3.3.1。复杂和DNA之间的绑定模式</gydF4y2Bah6>
<p>根据热力学数据,解释如下,药物与生物分子相互作用的模型可以(<gydF4y2Baa href="#B47">48</gydF4y2Baa>)(1)<gydF4y2Basvg height="11.0625" id="M59" style="vertical-align:-0.30096pt;width:52.25px;" version="1.1" viewbox="0 0 52.25 11.0625" width="52.25" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
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Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="23.405617" y="0">
<</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="35.446007" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="11.0625" id="M60" style="vertical-align:-0.30096pt;width:48.775002px;" version="1.1" viewbox="0 0 48.775002 11.0625" width="48.775002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
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Δ</gydF4y2Batspan>
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</gydF4y2Batspan>
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<</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="32.657837" y="0">
0</gydF4y2Batspan>
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</svg>疏水的力量;(2)<gydF4y2Basvg height="11.0625" id="M61" style="vertical-align:-0.30096pt;width:52.25px;" version="1.1" viewbox="0 0 52.25 11.0625" width="52.25" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
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Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="23.405617" y="0">
></gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="35.446007" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="11.0625" id="M62" style="vertical-align:-0.30096pt;width:48.775002px;" version="1.1" viewbox="0 0 48.775002 11.0625" width="48.775002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="20.617447" y="0">
></gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="32.657837" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>、范德华相互作用和氢键;(3)<gydF4y2Basvg height="11.0625" id="M63" style="vertical-align:-0.30096pt;width:52.25px;" version="1.1" viewbox="0 0 52.25 11.0625" width="52.25" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
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<tspan style="font-size: 12.50px; " x="23.405617" y="0">
></gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="35.446007" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="11.0625" id="M64" style="vertical-align:-0.30096pt;width:48.775002px;" version="1.1" viewbox="0 0 48.775002 11.0625" width="48.775002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="20.617447" y="0">
<</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="32.657837" y="0">
0</gydF4y2Batspan>
</text>
</g>
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</svg>静电相互作用[<gydF4y2Baa href="#B48">49</gydF4y2Baa>]。为了阐明我们复杂的与DNA的相互作用的热力学参数进行了计算。的情节<gydF4y2Basvg height="10.8" id="M65" style="vertical-align:-0.0pt;width:30.65px;" version="1.1" viewbox="0 0 30.65 10.8" width="30.65" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.8)">
<g transform="translate(72,-63.36)">
<text transform="matrix(1,0,0,-1,-71.95,63.41)">
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l</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="3.4758339" y="0">
n</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="13.890833" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>与<gydF4y2Basvg height="10.9125" id="M66" style="vertical-align:-0.17555pt;width:23.375px;" version="1.1" viewbox="0 0 23.375 10.9125" width="23.375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.9125)">
<g transform="translate(72,-63.27)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
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<tspan style="font-size: 12.50px; " x="9.727334" y="0">
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</text>
</g>
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</svg>(图<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig4/" target="_blank">4</gydF4y2Baa>;(<gydF4y2Baa href="#EEq4">4</gydF4y2Baa>)允许的决心<gydF4y2Basvg height="10.6625" id="M67" style="vertical-align:-0.0pt;width:26.4625px;" version="1.1" viewbox="0 0 26.4625 10.6625" width="26.4625" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.6625)">
<g transform="translate(72,-63.47)">
<text transform="matrix(1,0,0,-1,-71.95,63.52)">
<tspan style="font-size: 12.50px; " x="0" y="0">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="10.8125" id="M68" style="vertical-align:-0.1254pt;width:22.6625px;" version="1.1" viewbox="0 0 22.6625 10.8125" width="22.6625" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.8125)">
<g transform="translate(72,-63.35)">
<text transform="matrix(1,0,0,-1,-71.95,63.52)">
<tspan style="font-size: 12.50px; " x="0" y="0">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>。如果温度不发生显著的变化,焓的变化可以被看作是一个常数。根据绑定常数在不同的温度下,自由能变化可以估计(表<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/tab3/" target="_blank">3</gydF4y2Baa>;(<gydF4y2Baa href="#EEq4">5</gydF4y2Baa>)由以下方程<gydF4y2Baspan class="equation" id="EEq4">
<svg height="58.287498" id="M69" preserveaspectratio="xMaxYMin" style="vertical-align:-0pt;width:700.9375px;" version="1.1" viewbox="0 0 700.9375 58.287498" width="700.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,58.2875)">
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l</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="7.6393328" y="0">
n</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="15.966331" y="0">
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<tspan style="font-size: 12.50px; " x="28.969452" y="0">
=</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="40.997337" y="0">
−</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="50.77" y="-7.25">
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<g>
<path d="m 204.79,59.4 21.07,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path>
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<tspan style="font-size: 12.50px; " x="36.16" y="-16">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="45.187164" y="-16">
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</text>
<g>
<path d="m 242.37,59.4 18.13,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path>
</g>
<text transform="matrix(1,0,0,-1,242.37,47.52)">
<tspan style="font-size: 12.50px; " x="19.32" y="-8.75">
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)</gydF4y2Batspan>
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Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="-104.20284" y="17.719999">
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<tspan style="font-size: 12.50px; " x="-91.562302" y="17.719999">
=</gydF4y2Batspan>
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<tspan style="font-size: 12.50px; " x="-70.494743" y="17.719999">
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l</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="28.416487" y="17.719999">
n</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="34.667988" y="17.719999">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="44.195274" y="17.719999">
,</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="231.75" y="18.34">
(</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="235.9135" y="18.34">
5</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="242.16499" y="18.34">
)</gydF4y2Batspan>
</text>
</g>
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</svg></span>在哪里<gydF4y2Basvg height="10.325" id="M71" style="vertical-align:-0.0pt;width:13.2875px;" version="1.1" viewbox="0 0 13.2875 10.325" width="13.2875" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>是在相应的温度和Stern-Volmer猝灭常数吗<gydF4y2Basvg height="10.325" id="M72" style="vertical-align:-0.0pt;width:11.6875px;" version="1.1" viewbox="0 0 11.6875 10.325" width="11.6875" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
</g>
</g>
</svg>气体常数。当我们运用这一分析与CT-DNA复杂的绑定,我们发现<gydF4y2Basvg height="11.0625" id="M73" style="vertical-align:-0.30096pt;width:52.25px;" version="1.1" viewbox="0 0 52.25 11.0625" width="52.25" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="23.405617" y="0">
></gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="35.446007" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>和<gydF4y2Basvg height="11.0625" id="M74" style="vertical-align:-0.30096pt;width:48.775002px;" version="1.1" viewbox="0 0 48.775002 11.0625" width="48.775002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="20.617447" y="0">
></gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="32.657837" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>。因此,范德瓦耳斯相互作用可能是主力的绑定CT-DNA调查复杂。</gydF4y2Bap>
<div class="floats-partial-section partial-other-wrapper" data-section="tab3">
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表3</gydF4y2Baspan>
<a aria-label="View full page" class="caption-partial-url" href="//www.newsama.com/journals/bca/2011/429241/tab3/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
热力学参数的绑定CT-DNA Pt (II)复杂。</gydF4y2Badiv>
<a class="table-thumb-overlay" href="//www.newsama.com/journals/bca/2011/429241/tab3" target="_blank" aria-label="View full page"></a>
</div>
</div>
<h5 id="sec3.4">3.4。电化学行为的复杂CT-DNA的缺失和存在</gydF4y2Bah5>
<p>最近,电化学技术广泛被用作一个简单的和快速的方法来研究DNA相互作用与不同的化合物。铂是众所周知的电化学行为和强烈影响了电极材料。一个定义良好的观察和敏感峰从复杂的解决方案一个GC电极而不是工党。因此,一个GC电极用于这个调查。当CT-DNA添加到解决方案的复杂,阳极和阴极峰电流高度复杂的以同样的方式减少日益增加的DNA,(图<gydF4y2Baa href="//www.newsama.com/journals/bca/2011/429241/fig7/" target="_blank">7</gydF4y2Baa>)。还在DNA,阳极峰电位(Epa),阴极峰电位(Epc)和<gydF4y2Basvg height="14.3875" id="M79" style="vertical-align:-3.25793pt;width:25.887501px;" version="1.1" viewbox="0 0 25.887501 14.3875" width="25.887501" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3875)">
<g transform="translate(72,-60.49)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
</gydF4y2Batspan>
</text>
<text transform="matrix(1,0,0,-1,-63.02,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">
1</gydF4y2Batspan>
<tspan style="font-size: 8.75px; " x="4.3759999" y="0">
/</gydF4y2Batspan>
<tspan style="font-size: 8.75px; " x="6.8090558" y="0">
2</gydF4y2Batspan>
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</svg>(计算的平均Epc和Epa)所有显示积极的转变。这些积极的转变视为依据夹层复杂的DNA,因为这种相互作用是由于疏水作用。从其他的角度来看,如果一个分子结合静电带负电荷的deoxyribose-phosphate骨干的DNA,负峰电位的变化应该被检测到。因此,复杂的简历峰电位是积极的转变表明intercalative绑定模式的复杂与DNA (<gydF4y2Baa href="#B24">25</gydF4y2Baa>]。</gydF4y2Bap>
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<span class="caption-text">图7</gydF4y2Baspan>
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<td>Pt(2)复杂循环voltametry (10<gydF4y2Basup>−3</gydF4y2Basup>mol / L)在不同浓度的DNA的存在。</gydF4y2Batd>
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<h4 id="conclusion">4所示。结论</gydF4y2Bah4>
<p>在本文中,我们研究合成和表征的新铂(II)复杂,并结合CT-DNA进行了研究。结果表明,通过夹层复杂的DNA结合模式。分光光度法显示4海里的吸收带红移280海里一起重大减色性吸收带的复杂。intristic绑定常数(<gydF4y2Basvg height="18.012501" id="M80" style="vertical-align:-3.2316pt;width:83.675003px;" version="1.1" viewbox="0 0 83.675003 18.012501" width="83.675003" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,18.0125)">
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=</gydF4y2Batspan>
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×</gydF4y2Batspan>
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1</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="38.096642" y="0">
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</svg>米<gydF4y2Basup>−1</gydF4y2Basup>)是更符合intercalators并建议这个绑定模式。粘度测定表明,complex-DNA交互可以疏水和确认夹层。此外,复杂的感应探测CT-DNA CD谱的变化。荧光的研究显示,可能的荧光猝灭机理复杂的CT-DNA是静态猝灭。热力学参数(<gydF4y2Basvg height="11.0625" id="M81" style="vertical-align:-0.30096pt;width:52.25px;" version="1.1" viewbox="0 0 52.25 11.0625" width="52.25" xmlns="http://www.w3.org/2000/svg">
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<g transform="translate(72,-63.15)">
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<tspan style="font-size: 12.50px; " x="0" y="0">
Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
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<tspan style="font-size: 12.50px; " x="23.405617" y="0">
></gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="35.446007" y="0">
0</gydF4y2Batspan>
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</svg>和<gydF4y2Basvg height="11.0625" id="M82" style="vertical-align:-0.30096pt;width:48.775002px;" version="1.1" viewbox="0 0 48.775002 11.0625" width="48.775002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
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Δ</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">
</gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="20.617447" y="0">
></gydF4y2Batspan>
<tspan style="font-size: 12.50px; " x="32.657837" y="0">
0</gydF4y2Batspan>
</text>
</g>
</g>
</svg>)表明,范德瓦耳斯相互作用发生夹层模式。同时,简历结果证实这种模式,因为随着CT-DNA浓度增加,转向更高的潜在的观察。</gydF4y2Bap>
<h4 id="acknowledgment">承认</gydF4y2Bah4>
<p>金融Razi大学研究中心的支持。</gydF4y2Bap>
</div>
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2204年</gydF4y2Badiv>
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