克隆、表达大肠杆菌,和净化的密码子优化,登革2信封域三世。(一)蛋白表达和纯化的sds - page显示几个步骤。1、总溶解产物的细胞在诱导;2、总溶解产物IPTG诱导后的细胞;3、蛋白质分子量标准;4、可溶性内含体之前加载到Ni-NTA列;5,列材料;6 - 7,连续两个洗;8 - 9,连续两个洗脱。(b)活性的纯化和复合重组领域三世DENV2州立细胞。 Protein was refolded using several refolding conditions (RB1 to RB9) before dialysis and filtration for testing against the virus, in parallel with virus alone and ribavirin as a control. Data is presented as the mean and SD of two independent experiments. Condition RB1 was not used in this experiment as protein consistently precipitated.