PDF
开放获取
拿王,克劳迪娅威尔金,阿尔弗雷德·烈性黑啤酒Bernhard Tribukait, ”评价肿瘤异质性的前列腺癌DNA血细胞计数和组织病理学分级流——和图像”,分析细胞病理学, 卷。20., 文章的ID489303年, 14 页面, 2000年。 https://doi.org/10.1155/2000/489303
评价肿瘤异质性的前列腺癌DNA血细胞计数和组织病理学分级流——和图像
文摘
背景。异质性的前列腺癌肿瘤侵犯预处理低估的原因之一。我们研究肿瘤异质性和肿瘤的概率最高的等级和DNA异倍体性与活检的数量关系。材料和方法。核心活检标本模拟从五个随机选择的肿瘤领域的16个烈性黑啤酒的二级和三级23前列腺癌肿瘤分级和DNA倍性分析了流量检测和荧光图像血细胞计数(FCM, FICM)。细胞周期成分被FCM测量。结果。通过倍性和细胞周期成分测定,形态定义肿瘤可以进一步细分。异质性肿瘤的年级和DNA倍性(FCM)是54%和50%。共存的二倍体肿瘤细胞非整倍体标本代表肿瘤异质性的另一种形式。 The proportion of diploid tumor cells decreased significantly with tumor grade and with increase in the fraction of proliferating cell of the aneuploid tumor part. The probability of estimating the highest tumor grade or aneuploidy increased from 40% for one biopsy to 95% for 5 biopsies studied. By combining the tumor grade with DNA ploidy, the probability of detecting a highly aggressive tumor increased from 40% to 70% and 90% for one and two biopsies, respectively. Conclusion. Specimens of the size of core biopsies can be used for evaluation of DNA ploidy and cell cycle composition. Underestimation of aggressiveness of prostate carcinoma due to tumor heterogeneity is minimized by simultaneous study of the tumor grade and DNA ploidy more than by increasing the number of biopsies. The biological significance of coexistent diploid tumor cell in aneuploid lesions remains to be evaluated.
版权
版权©2000 Hindawi出版公司。这是一个开放的分布式下文章知识共享归属许可,它允许无限制的使用、分配和复制在任何媒介,提供最初的工作是正确引用。
