通过MALDI质谱法‣可能性和局限性来自生物材料的蛋白质的共价结构的表征

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基质辅助激光解吸/电离质谱（MALDI-MS）已成为用于从生物材料中分离的蛋白质的共价结构的详细表征的主要手段，由于其下面的电势的主要是：高灵敏度和特异性，分析速度，appropriateness for mixture analysis, high tolerance towards contaminants, and compatibility with separation techniques, e.g., gel electrophoresis. These characteristics enable the structural analysis of proteins even if they are only available in limited amounts and/or in mixtures, and even if the protein preparations contain large amounts of salts, buffers, detergents and denaturants. Additionally, structural data can be generated within a relatively short time.Whereas X-ray crystallography and multidimensional NMR techniques can provide “absolute” structural data, i.e., a three-dimensional “picture” of the protein of interest, MALDI-MS-especially in combination with selective protein chemistry – yields information on particular aspects of the entire protein structure, e.g., primary structure, active site(s), binding sites, and posttranslational modifications, all of which are often of crucial interest for the understanding of the protein function. Taking into account that protein crystallography and protein NMR studies require large quantities of highly purified sample, MALDI-MS can be even more regarded as a powerful complement in protein structure analysis.This review aims at describing the state-of-the-art of MALDI-MS for characterisation of proteins from biological material by evaluating its potential and limitations.

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通过MALDI质谱法‣可能性和局限性来自生物材料的蛋白质的共价结构的表征

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