). The sensitivity and specificity of ELISA test used for evaluating the responses of human total IgG to different hydatid cyst fluid (HCF) antigens among the case and control groups were 100 and 95.8%, respectively. Cross-reaction of human IgG class and subclasses responses was found almost for all the antigens with the best reaction against human and cattle (HCF) antigens and antigen B using a ratio of mean OD value to each antigen divided by the cut-off point value for the same antigen. Human sera showed a considerable cross-reactivity against all antigens by using ELISA.">
人与动物源棘球蚴粗抗原对人IgG类和亚类的交叉反应据/title>
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no-repeat 6px;background-position:center;margin-top:10px;min-width:55px;display:none;} @media (min-width:768px){.hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption .expand_btn{display:none;}} .hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-text{-webkit-flex:9;-ms-flex:9;flex:9;text-align:left;padding:21px 37px 10px 0px;font-size:16px;font-weight:700;} @media (min-width:768px){.hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-text{padding:19px 18px 10px 18px;}} @media (min-width:992px){.hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-text{padding:19px 21px 10px 21px;}} .hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption 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no-repeat 6px;background-position:center;padding:16px 0 16px 0;min-width:62px;max-width:62px;} @media (min-width:768px){.hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-partial-url{padding:16px 0 17px 0;min-width:52px;max-width:52px;}} @media (min-width:992px){.hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-partial-url{padding:15px 0 17px 0;min-width:60px;max-width:60px;}} .hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption .caption-partial-url:hover{-webkit-text-decoration:none;text-decoration:none;} .hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption-text{font-size:14px;padding:0px 18px 17px 22px;} @media (min-width:768px){.hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption-text{padding:0px 15px 15px 18px;}} @media (min-width:992px){.hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption-text{padding:0px 18px 19px 21px;}} .hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption-text figcaption{margin:0;padding:0;} .hDZsgu .floats-partial-section .floats-partial-footer .floats-partial-caption-text svg{max-width:100%;} .hDZsgu ol{font-size:16px;padding-left:32px;font-family:STIXGeneral-Regular;line-height:24px;margin:15px 0;} .hDZsgu ol li{margin:10px 0;padding-left:10px;word-break:normal;} .hDZsgu ol li a{word-break:break-all;} .hDZsgu ol li .reflinks{display:block;} .hDZsgu ol li .reflinks a{color:#4D8A17;-webkit-text-decoration:none;text-decoration:none;cursor:pointer;} .hDZsgu ol li .reflinks a:hover{-webkit-text-decoration:underline;text-decoration:underline;} .hDZsgu ol li .reflinks .sep{color:#000000;} .hDZsgu .table-wrapper{width:auto;overflow-x:auto;} .hDZsgu .table-wrapper::-webkit-scrollbar{-webkit-appearance:none;width:5px;height:5px;} .hDZsgu .table-wrapper::-webkit-scrollbar-thumb{border-radius:5px;background-color:rgba(0,0,0,.5);-webkit-box-shadow:0 0 1px rgba(255,255,255,.5);} @media (min-width:768px){.hDZsgu .table-wrapper{overflow-x:auto;width:auto;-webkit-break-word:word-break;break-word:word-break;margin:0 auto;}} @media (min-width:992px){.hDZsgu .table-wrapper{width:auto;}} @media (min-width:1324px){.hDZsgu .table-wrapper{width:auto;}} @media (min-width:320px){.hDZsgu{margin-bottom:33px;padding:0;}} @media (min-width:992px){.hDZsgu{padding:0;margin-bottom:100px;}} @media (min-width:1325px){.hDZsgu{margin-bottom:100px;}} .hDZsgu .owl-stage{display:-webkit-box;display:-webkit-flex;display:-ms-flexbox;display:flex;} .hDZsgu .floats-partial-middle--thumbs{display:-webkit-box;display:-webkit-flex;display:-ms-flexbox;display:flex;} .hDZsgu .floats-partial-middle--thumbs .partial-carousel-dot svg{fill:#4D8A17;} .hDZsgu .floats-partial-middle--thumbs .partial-carousel-dot strong{color:#4D8A17;font-weight:500;} .hDZsgu .floats-partial-middle--thumbs .partial-carousel-dot.active strong{color:#007B8B;} .hDZsgu 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<p>Afra Khosravi，Sobhan Ghafourian，Morteza Shamsi，Nourkhoda Sadeghifard，Abbas Maleki，Ebrahim Babaahmadi据!-- -->那据!-- -->
<!-- -->“据span class="adjust-article-svg-size">人与动物源棘球蚴粗抗原对人IgG类和亚类的交叉反应据/span>“，据!-- --><i>寄生虫学研究杂志据/i>那据!-- -->
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<h1 class="sc-iRbamj jZOxOO sc-iSDuPN cTnPQL article_title adjust-article-svg-size heading-title">人与动物源棘球蚴粗抗原对人IgG类和亚类的交叉反应据/h1>
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<div class="sc-jrIrqw eHMFfo article_authors">
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<span class="">afra khosravi.据!-- -->那据sup>1据!-- -->那据!-- -->2据/sup></span>
<span class="">索班Ghafourian据!-- -->那据sup>2据/sup></span>
<span class="">名沙姆西据!-- -->那据sup>3.据/sup></span>
<span class=""><b>Nourkhoda Sadeghifard据/b><a class="sc-htpNat bUhGXt link" href="mailto:" aria-label="Mail Option"><span class="sc-htoDjs kNoiZu sc-hjRWVT irNWJI icon-colored _2zt-1j0LmaF8UjTQAM413G icon-themeable" title="" font-size="16"></span></a>那据sup>2据/sup></span>
<span class="">阿巴斯Maleki据!-- -->那据sup>2据/sup>
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<span class="">Ebrahim Babaahmadi据sup>3.据/sup></span>
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<p><sup>1据/sup><span>伊拉姆医科大学医学院免疫学系，伊朗伊拉姆6931994585据/span></p>
<p><sup>2据/sup><span>伊朗伊拉姆医科大学临床微生物研究中心，伊朗伊拉姆6931994585据/span></p>
<p><sup>3.据/sup><span>伊拉姆大学兽医学院寄生虫学系，伊朗伊拉姆6931994585据/span></p>
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<span class="articleContent__PublicationValue-sc-2yl9jy-7 khHUcu">2011年11月07据/span>
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<span class="articleContent__PublicationValue-sc-2yl9jy-7 khHUcu">2011年12月31日据/span>
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<span class="articleContent__PublicationValue-sc-2yl9jy-7 khHUcu">2012年1月2日据/span>
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<span class="articleContent__PublicationField-sc-2yl9jy-6 cIribm">发表据/span>
<span class="articleContent__PublicationValue-sc-2yl9jy-7 khHUcu">2012年3月13日据/span>
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<article class="sc-jtRlXQ hDZsgu article_body ">
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<h4 class="header" id="abstract">摘要据/h4>
<p>本工作旨在评价人免疫血清对绵羊、人、小鼠、牛包虫液粗抗原以及囊液抗原B片段的交叉反应性。用含原头节的羊包虫囊液抗原感染30只balb/c小鼠，观察原头节的生存能力。采用单因素方差分析(ANOVA)检验病例组与对照组光密度(OD)值均值的差异。人IgG类抗体抗B抗原最高(0.93)，抗牛HCF抗原最低(0.32)。对这些抗原反应的差异具有统计学意义(据svg height="11.0625" id="M1" style="vertical-align:-0.30096pt;width:63.962502px;" version="1.1" viewbox="0 0 63.962502 11.0625" width="63.962502" xmlns="http://www.w3.org/2000/svg">
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</svg>）.用于评估人总IgG对不同纳米胱囊液（HCF）抗原的反应的ELISA试验的敏感性和特异性分别为100和95.8％。几乎针对人和牛（HCF）抗原和抗原B的所有抗原发现所有抗原的人IgG类和亚类反应的交叉反应使用平均OD值与每个抗原的比例除以截止点值对于相同的抗原。人血清通过使用ELISA对所有抗原显示相当大的交叉反应性。据/p>
<span class="end-abs"></span>
<h4 id="introduction">1.介绍据/h4>
<p>包虫病是人类和家畜或野生动物的一种慢性、囊肿形成的寄生虫病。它是由属于该属的狗/狐狸绦虫(绦虫)的幼虫阶段感染引起的据i>棘球绦虫据/i>(绦虫科)，亦称包虫病[据a href="#B1">1据/a>］.对医学和公共卫生的重要性主要有两种据i>棘球绦虫granulosus据/i>和据i>棘球绦虫multilocularis据/i>分别引起囊性棘球蚴病(CE)和肺泡型棘球蚴病(AE)。人类棘球蚴是最常见的表现，可能占全球估计300万病例的95%以上。人类AE每年造成大约30万至50万例病例(均在北半球)[据a href="#B1">1据/a>］.由于这些寄生虫是复杂的多细胞病原体，能够调节抗寄生虫免疫反应，并在它们的哺乳动物宿主中持续活跃，了解免疫系统如何处理这些寄生虫是一个主要的挑战[据a href="#B1">1据/a>］.虽然现代分子和免疫学方法的应用阐明了一些关于包虫病感染过程中产生的免疫反应的本质的见解，许多方面的据i>棘球绦虫据/i>主机相互作用仍未探索[据a href="#B1">1据/a>］.据/p>
<p>如果在大脑、心脏等重要器官形成包虫病，患病风险会更大。该病在世界上大多数国家普遍存在，特别是在绵羊饲养地区。这种疾病每年造成许多经济和健康问题[据a href="#B2">2据/a>那据a href="#B3">3.据/a>］.来自热带和亚热带地区，如地中海，欧洲，中亚，中东，远东，俄罗斯，澳大利亚，新西兰，美国和非洲的热带和亚热带地区报道了瓦塞纳米患病率较高。伊朗的所有省份报道了这种疾病，在Khorasan（每10万个种群4.45人口4.45）中，霍莫佐根（0.1人群中的4.45个）普遍存在，而全国疾病的总体普遍性是关于1.2人口100,000人。伊朗是一种感染的超流行区域据i>棘球绦虫granulosus据/i>[据a href="#B4">4.据/a>］.据/p>
<p>与狗和犬等家畜和野生动物有密切接触的农村人口，特别是生活在不发达国家的人，感染的风险更大。大约60%的包虫病病例是无症状的，因此这种疾病可能在人体内停留20年。由于寄生虫可寄生于肺、心、脑、肝、脾和脊髓等不同器官，诊断该病非常困难，通常基于血清学等临床旁方法[据a href="#B5">5.据/a>］.由于手术被认为是唯一可用于此类疾病的治疗，因此对患者的明确和立即诊断患者至关重要[据a href="#B6">6.据/a>］.用于诊断囊性包发状囊泡（CH）的粗ueAdahatid囊肿液（HCF）抗原是一种方法，其目前的血清学研究可以在疾病的快速治疗方面有助于且有效[据a href="#B7">7.据/a>］.似乎人类来源的抗原对诊断包虫病更有用，因为据报道，人类CE患者的HCF显示相对较强的阳性反应[据a href="#B8">8.据/a>］.人类HCF抗原的制备过于困难，不可能在任何地方、任何时间都适用。如果血清学诊断是基于使用某些高度有效和可靠的动物抗原而不是人类抗原，那么用这种抗原设计诊断试剂盒可能是一种简单、廉价和可用的方法。大多数用于诊断包虫病的血清学试验都有其自身的问题，如可用性有限，不同的敏感性，特异性或制备困难。其中一些测试需要几种特定的技术、设备和经验丰富的工作人员。据/p>
<p>血清学检测抗据i>棘球绦虫据/i>实验室常用血清抗体、酶联免疫吸附试验、间接hem凝集抗体试验(IHAT)和乳胶凝集试验(LAT) [据a href="#B8">8.据/a>]，而免疫荧光抗体试验（IFAT），免疫电泳（IEP），以及一些其他测试的频率较少。在许多国家，易于使用IgG-ELISA的材料，试剂和设备，并且这种技术可能是人CE免疫诊断的最佳选择。然而，在人CE的情况下，仍然没有标准，高度敏感和特异性的血清学检测[据a href="#B8">8.据/a>］.因此，在临床实践中，应注意血清学检测的结果取决于多种因素，如抗原质量、检测系统、器官部位、包虫病囊肿数量、免疫反应的个体变异性等。IgG-ELISA是目前最敏感的检测方法之一[据a href="#B9">9.据/a>］.IFAT的敏感性与ELISA-IgG相似。由于不同测试的灵敏度各不相同，许多化验所至少采用两种不同的初级测试来进行CE的常规诊断，这通常会增加敏感性[据a href="#B10">10据/a>那据a href="#B11">11据/a>］.不同的研究报道了ELISA的敏感性范围为60-90%，特异性范围为75-90% [据a href="#B12">12据/a>］.通常情况下，检测范围因抗原类型、方法、进行检测的地理区域以及疾病流行地区而异。有时在血清学试验中发生交叉反应，降低了诊断的准确性，而免疫印迹试验可以最好地证实这一点。另一方面，抗原有不同的组分;例如，抗原B有8、16、24和38kd，在疾病诊断中具有不同的敏感性[据a href="#B13">13据/a>那据a href="#B14">14据/a>］.此外，这些组分在每种动物中也有不同的敏感性;因此，找到一种对人或其他动物血清具有高敏感性和特异性的抗原，在开发一种可靠和廉价的HC诊断方法方面是一个相当大的进展。据/p>
<p>为了达到这一目标，首先要从不同来源(人或动物)制备一种能被大多数人和动物血清识别的适当的寄生虫粗抗原。下一步是对高质量、高质量的抗原进行纯化和鉴定。然而，在某些情况下，适当的粗抗原可能表明在血清学实验中制备某些抗原的可接受的效率。哪种抗原对人类抗体的反应更强烈、更有效?哪部分更具有免疫性?哪一种免疫球蛋白类或亚类反应最好?本研究的目的是根据适用于局部抗原条件的有效观察结果，对这些问题给出一些更清晰的答案(图)据a href="//www.newsama.com/journals/jpr/2012/947948/fig1/" target="_blank">1据/a>）.据/p>
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<span class="caption-text">图1据/span>
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SDS PAGE显示人类和动物HCFs的不同组分。兆瓦:分子量;H:人类;B: B抗原;AgK:抗原包虫病试剂盒;承宪:羊;M:小鼠;C:牛。据/table>
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<h4 id="material-and-methods">2.材料和方法据/h4>
<p>这是一项分析性病例对照研究，使用人和动物粗HCF作为抗原来源，进行ELISA、Western blotting和小鼠免疫。收集样本血清用于现在的工作从近期经历了包虫手术的病人在医院的德黑兰哈马丹,Ilam城市作为人类病例组与一些人类或动物血清没有包虫病的历史- HC用ELISA和IFAT作为对照组。用含羊HC原头节液感染30只balb/c小鼠，用Eozin法测定其活力。从自然感染的人、羊、牛以及小鼠包虫液囊肿中提取并制备所需抗原。据/p>
<h5 id="sec2.1">2.1。统计分析据/h5>
<p>用使用计算样本大小据svg height="10.9375" id="M2" style="vertical-align:-0.20064pt;width:51.362499px;" version="1.1" viewbox="0 0 51.362499 10.9375" width="51.362499" xmlns="http://www.w3.org/2000/svg">
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<tspan style="font-size: 12.50px; " x="22.555412" y="0">
5.据/tspan>
<tspan style="font-size: 12.50px; " x="28.806911" y="0">
％据/tspan>
<tspan style="font-size: 12.50px; " x="38.146652" y="0">
那据/tspan>
<tspan style="font-size: 12.50px; " x="47.523903" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="57.97641" y="0">
=据/tspan>
<tspan style="font-size: 12.50px; " x="70.0168" y="0">
1据/tspan>
<tspan style="font-size: 12.50px; " x="76.268303" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="82.519798" y="0">
％据/tspan>
</text>
</g>
</g>
</svg>,据svg height="11.1" id="M6" style="vertical-align:-0.17555pt;width:35.799999px;" version="1.1" viewbox="0 0 35.799999 11.1" width="35.799999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.1)">
<g transform="translate(72,-63.12)">
<text transform="matrix(1,0,0,-1,-71.95,63.35)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.239957" y="0">
=据/tspan>
<tspan style="font-size: 12.50px; " x="22.280346" y="0">
5.据/tspan>
</text>
</g>
</g>
</svg>团体，是15.4），据svg height="20.1875" id="M7" style="vertical-align:-3.80836pt;width:151.4375px;" version="1.1" viewbox="0 0 151.4375 20.1875" width="151.4375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,20.1875)">
<g transform="translate(72,-55.85)">
<text transform="matrix(1,0,0,-1,-71.95,59.7)">
<tspan style="font-size: 12.50px; " x="28.700001" y="0.029999999">
∑据/tspan>
<tspan style="font-size: 12.50px; " x="0" y="0">
Δ据/tspan>
<tspan style="font-size: 12.50px; " x="12.490497" y="0">
=据/tspan>
<tspan style="font-size: 12.50px; " x="24.530886" y="0">
（据/tspan>
</text>
<text transform="matrix(1,0,0,-1,-31.82,65.93)">
<tspan style="font-size: 8.75px; " x="0" y="0">
5.据/tspan>
<tspan style="font-size: 8.75px; " x="0" y="9.9399996">
据/tspan>
<tspan style="font-size: 8.75px; " x="2.7218721" y="9.9399996">
=据/tspan>
<tspan style="font-size: 8.75px; " x="8.7169924" y="9.9399996">
1据/tspan>
</text>
<text transform="matrix(1,0,0,-1,-16.15,59.7)">
<tspan style="font-size: 12.50px; " x="0" y="0">
（据/tspan>
<tspan style="font-size: 12.50px; " x="4.1634989" y="0">
据/tspan>
</text>
<text transform="matrix(1,0,0,-1,-4.74,56.57)">
<tspan style="font-size: 8.75px; " x="0" y="0">
据/tspan>
</text>
<text transform="matrix(1,0,0,-1,1.26,59.7)">
<tspan style="font-size: 12.50px; " x="0" y="0">
−据/tspan>
</text>
<g>
<path d="m 12.6,67.94 7.61,0" style="fill:none;stroke:#000000;stroke-width:0.82499999;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:10;stroke-opacity:1;stroke-dasharray:none"></path>
</g>
<text transform="matrix(1,0,0,-1,12.6,59.7)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="7.6018238" y="0">
）据/tspan>
</text>
<text transform="matrix(1,0,0,-1,24.38,65.89)">
<tspan style="font-size: 8.75px; " x="0" y="0">
2据/tspan>
</text>
<text transform="matrix(1,0,0,-1,29.25,59.7)">
<tspan style="font-size: 12.50px; " x="0" y="0">
）据/tspan>
<tspan style="font-size: 12.50px; " x="4.1634989" y="0">
/据/tspan>
<tspan style="font-size: 12.50px; " x="7.6393328" y="0">
据/tspan>
</text>
<text transform="matrix(1,0,0,-1,44.23,64.7)">
<tspan style="font-size: 8.75px; " x="0" y="0">
2据/tspan>
</text>
</g>
</g>
</svg>,据svg height="10.8375" id="M8" style="vertical-align:-3.2316pt;width:13.2px;" version="1.1" viewbox="0 0 13.2 10.8375" width="13.2" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.8375)">
<g transform="translate(72,-63.33)">
<text transform="matrix(1,0,0,-1,-71.95,66.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
</text>
<text transform="matrix(1,0,0,-1,-64.71,63.47)">
<tspan style="font-size: 8.75px; " x="0" y="0">
据/tspan>
</text>
</g>
</g>
</svg>为五组平均OD值。采用方差分析检验各组OD值的差异均数。用Levens统计量进行方差齐性检验。事后分析采用Tukey检验。据svg height="10.325" id="M9" style="vertical-align:-0.0pt;width:11.75px;" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
</text>
</g>
</g>
</svg>-值小于5%被认为具有统计学意义。据/p>
<h4 id="results">3.结果据/h4>
<h5 id="sec3.1">3．1.原头节感染小鼠HC形成的实验结果据/h5>
<p>实验感染小鼠在接受羊源原头节6 ~ 9个月后均出现棘球蚴囊肿。在小鼠中，HC的形成量和形成率分别在肝脏和腹部最高。30只balb/c小鼠中有10只在6-9个月后感染，30只ICR小鼠中有30只在6-9个月后出现HC。据/p>
<h5 id="sec3.2">３．２．ELISA结果使用人血清据/h5>
<p>对病例组和对照组的30份人血清，采用ELISA法测定抗人、小鼠、绵羊和牛源HCFs抗原的总IgG和IgG亚类抗体(IgG2、IgG3和IgG4)及抗原B。分别计算每个抗体的临界值，分别计算每个抗原的敏感性和特异性(表)据a href="//www.newsama.com/journals/jpr/2012/947948/tab1/" target="_blank">1据/a>那据a href="//www.newsama.com/journals/jpr/2012/947948/tab2/" target="_blank">2据/a>那据a href="//www.newsama.com/journals/jpr/2012/947948/tab3/" target="_blank">3.据/a>那据a href="//www.newsama.com/journals/jpr/2012/947948/tab4/" target="_blank">4.据/a>那据a href="//www.newsama.com/journals/jpr/2012/947948/tab5/" target="_blank">5.据/a>,据a href="//www.newsama.com/journals/jpr/2012/947948/tab6/" target="_blank">6.据/a>）.据/p>
<div class="floats-partial-section partial-other-wrapper" data-section="tab1">
<div class="floats-partial-content fig-content-tab1">
<table class="table-group" id="tab1">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr class="thead">
<td align="left">OD据/td>
<td align="center">意思据/td>
<td align="center">Std.偏差据/td>
<td align="center">最低据/td>
<td align="center">最大据/td>
<td align="center">数量据/td>
<td align="center">范围据/td>
<td align="center">截止据/td>
</tr>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr>
<td align="left">免疫球蛋白据/td>
<td align="center">０．５９据/td>
<td align="center">０．０２据/td>
<td align="center">0.51据/td>
<td align="center">0.68据/td>
<td align="center">30.据/td>
<td align="center">0.08据/td>
<td align="center">0.08据/td>
</tr>
<tr>
<td align="left">IgG2据/td>
<td align="center">0.34据/td>
<td align="center">0.04据/td>
<td align="center">0.22据/td>
<td align="center">0.41据/td>
<td align="center">30.据/td>
<td align="center">0.19据/td>
<td align="center">0.12据/td>
</tr>
<tr>
<td align="left">IgG3据/td>
<td align="center">０．１６据/td>
<td align="center">0.04据/td>
<td align="center">0.12据/td>
<td align="center">0.38据/td>
<td align="center">30.据/td>
<td align="center">0.26据/td>
<td align="center">0.11据/td>
</tr>
<tr>
<td align="left">IgG4据/td>
<td align="center">1.58据/td>
<td align="center">0.18据/td>
<td align="center">1.31据/td>
<td align="center">1.99据/td>
<td align="center">30.据/td>
<td align="center">0.68据/td>
<td align="center">0．15据/td>
</tr>
<tr>
<td align="left">IgE据/td>
<td align="center">0.49据/td>
<td align="center">0．17据/td>
<td align="center">0．15据/td>
<td align="center">0.76据/td>
<td align="center">30.据/td>
<td align="center">0.62据/td>
<td align="center">0.06据/td>
</tr>
<tr class="table-tr">
<td colspan="8">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
<tr class="table-fn">
<td>
<svg height="13.6375" id="M10" style="vertical-align:-2.21957pt" version="1.1" viewbox="0 0 150.3 13.6375" width="150.3" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,13.6375)">
<g transform="translate(72,-61.09)">
<text transform="matrix(1,0,0,-1,-71.95,63.35)">
<tspan style="font-size: 12.50px; " x="0" y="0">
（据/tspan>
<tspan style="font-size: 12.50px; " x="4.1634989" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="15.053612" y="0">
=据/tspan>
<tspan style="font-size: 12.50px; " x="27.094002" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="33.345501" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="36.471252" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="42.722752" y="0">
1据/tspan>
<tspan style="font-size: 12.50px; " x="48.974251" y="0">
那据/tspan>
<tspan style="font-size: 12.50px; " x="58.351501" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="69.554192" y="0">
=据/tspan>
<tspan style="font-size: 12.50px; " x="81.594582" y="0">
7.据/tspan>
<tspan style="font-size: 12.50px; " x="87.846077" y="0">
2据/tspan>
<tspan style="font-size: 12.50px; " x="94.09758" y="0">
5.据/tspan>
<tspan style="font-size: 12.50px; " x="100.34908" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="103.47483" y="0">
3.据/tspan>
<tspan style="font-size: 12.50px; " x="109.72633" y="0">
8.据/tspan>
<tspan style="font-size: 12.50px; " x="115.97783" y="0">
）据/tspan>
</text>
</g>
</g>
</svg>。据br></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表1据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab1/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
30例自然感染的hyadid患者对人囊性粗液抗原的IgG类和亚类反应OD值的平均值据/div>
</div>
</div>
<div class="floats-partial-section partial-other-wrapper" data-section="tab2">
<div class="floats-partial-content fig-content-tab2">
<table class="table-group" id="tab2">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr class="thead">
<td align="left">OD据/td>
<td align="center">意思据/td>
<td align="center">Std.偏差据/td>
<td align="center">最低据/td>
<td align="center">最大据/td>
<td align="center">数量据/td>
<td align="center">范围据/td>
<td align="center">截止据/td>
</tr>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr>
<td align="left">免疫球蛋白据/td>
<td align="center">0.04据/td>
<td align="center">０．０２据/td>
<td align="center">0.01据/td>
<td align="center">0.11据/td>
<td align="center">30.据/td>
<td align="center">0．1据/td>
<td align="center">0.08据/td>
</tr>
<tr>
<td align="left">IgG2据/td>
<td align="center">0.04据/td>
<td align="center">0.04据/td>
<td align="center">0.据/td>
<td align="center">０．１３据/td>
<td align="center">30.据/td>
<td align="center">０．１３据/td>
<td align="center">0.12据/td>
</tr>
<tr>
<td align="left">IgG3据/td>
<td align="center">0.05据/td>
<td align="center">0.03据/td>
<td align="center">0.01据/td>
<td align="center">0.12据/td>
<td align="center">30.据/td>
<td align="center">0.12据/td>
<td align="center">0.11据/td>
</tr>
<tr>
<td align="left">IgG4据/td>
<td align="center">0.09据/td>
<td align="center">0.04据/td>
<td align="center">0.据/td>
<td align="center">0．15据/td>
<td align="center">30.据/td>
<td align="center">0．15据/td>
<td align="center">0．15据/td>
</tr>
<tr>
<td align="left">IgE据/td>
<td align="center">０．０２据/td>
<td align="center">0.04据/td>
<td align="center">0.据/td>
<td align="center">06据/td>
<td align="center">30.据/td>
<td align="center">06据/td>
<td align="center">06据/td>
</tr>
<tr class="table-tr">
<td colspan="8">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
<tr class="table-fn">
<td>
<svg height="10.9125" id="M11" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 56.150002 10.9125" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.9125)">
<g transform="translate(72,-63.27)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
=据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>;据i>F据/i>= 15.98。据br></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表2据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab2/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
30例健康人对人囊性粗液抗原IgG类和亚类反应的OD值平均值。据/div>
</div>
</div>
<div class="floats-partial-section partial-other-wrapper" data-section="tab3">
<div class="floats-partial-content fig-content-tab3">
<table class="table-group" id="tab3">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr class="thead">
<td align="left">OD据/td>
<td align="center">意思据/td>
<td align="center">Std.偏差据/td>
<td align="center">最低据/td>
<td align="center">最大据/td>
<td align="center">数量据/td>
<td align="center">范围据/td>
<td align="center">截止据/td>
</tr>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr>
<td align="left">免疫球蛋白据/td>
<td align="center">0.34据/td>
<td align="center">0.06据/td>
<td align="center">０．３据/td>
<td align="center">0.38据/td>
<td align="center">30.据/td>
<td align="center">0.26据/td>
<td align="center">0.12据/td>
</tr>
<tr>
<td align="left">IgG2据/td>
<td align="center">0.21据/td>
<td align="center">0．1据/td>
<td align="center">0.08据/td>
<td align="center">0.42据/td>
<td align="center">30.据/td>
<td align="center">０．３３据/td>
<td align="center">0.08据/td>
</tr>
<tr>
<td align="left">IgG3据/td>
<td align="center">0．25据/td>
<td align="center">０．１６据/td>
<td align="center">0.03据/td>
<td align="center">0.71据/td>
<td align="center">30.据/td>
<td align="center">0.68据/td>
<td align="center">0.11据/td>
</tr>
<tr>
<td align="left">IgG4据/td>
<td align="center">0．7据/td>
<td align="center">0.12据/td>
<td align="center">0.51据/td>
<td align="center">0.87据/td>
<td align="center">30.据/td>
<td align="center">0.36据/td>
<td align="center">0．14据/td>
</tr>
<tr>
<td align="left">IgE据/td>
<td align="center">0．31据/td>
<td align="center">0.08据/td>
<td align="center">0.22据/td>
<td align="center">0.48据/td>
<td align="center">30.据/td>
<td align="center">0.26据/td>
<td align="center">0.12据/td>
</tr>
<tr class="table-tr">
<td colspan="8">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表3据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab3/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
30例自然感染hyadid患者对绵羊囊性粗液抗原的IgG类和亚类反应OD值的平均值。据/div>
</div>
</div>
<div class="floats-partial-section partial-other-wrapper" data-section="tab4">
<div class="floats-partial-content fig-content-tab4">
<table class="table-group" id="tab4">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr class="thead">
<td align="left">OD据/td>
<td align="center">意思据/td>
<td align="center">Std.Deviation据/td>
<td align="center">最低据/td>
<td align="center">最大据/td>
<td align="center">数量据/td>
<td align="center">范围据/td>
<td align="center">截止据/td>
</tr>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr>
<td align="left">免疫球蛋白据/td>
<td align="center">0.35据/td>
<td align="center">0．14据/td>
<td align="center">0.27据/td>
<td align="center">0.43据/td>
<td align="center">30.据/td>
<td align="center">0．5据/td>
<td align="center">0.06据/td>
</tr>
<tr>
<td align="left">IgG2据/td>
<td align="center">0．14据/td>
<td align="center">０．０２据/td>
<td align="center">0.12据/td>
<td align="center">0．17据/td>
<td align="center">30.据/td>
<td align="center">0.05据/td>
<td align="center">0.12据/td>
</tr>
<tr>
<td align="left">IgG3据/td>
<td align="center">0.12据/td>
<td align="center">0.004据/td>
<td align="center">0.11据/td>
<td align="center">0.12据/td>
<td align="center">30.据/td>
<td align="center">0.01据/td>
<td align="center">０．１６据/td>
</tr>
<tr>
<td align="left">IgG4据/td>
<td align="center">0.29据/td>
<td align="center">0.36据/td>
<td align="center">0.07据/td>
<td align="center">0.97据/td>
<td align="center">30.据/td>
<td align="center">0．9据/td>
<td align="center">0.04据/td>
</tr>
<tr>
<td align="left">IgE据/td>
<td align="center">0.12据/td>
<td align="center">0.01据/td>
<td align="center">0．1据/td>
<td align="center">0．14据/td>
<td align="center">30.据/td>
<td align="center">0.04据/td>
<td align="center">0.11据/td>
</tr>
<tr class="table-tr">
<td colspan="8">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表4据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab4/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
30例自然感染hyadid患者对小鼠囊性粗液抗原的IgG类和亚类反应OD值的平均值。据/div>
</div>
</div>
<div class="floats-partial-section partial-other-wrapper" data-section="tab5">
<div class="floats-partial-content fig-content-tab5">
<table class="table-group" id="tab5">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr class="thead">
<td align="left">OD据/td>
<td align="center">意思据/td>
<td align="center">Std.偏差据/td>
<td align="center">最低据/td>
<td align="center">最大据/td>
<td align="center">数量据/td>
<td align="center">范围据/td>
<td align="center">截止据/td>
</tr>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr>
<td align="left">免疫球蛋白据/td>
<td align="center">0.93据/td>
<td align="center">0．17据/td>
<td align="center">0.81据/td>
<td align="center">1.04据/td>
<td align="center">30.据/td>
<td align="center">0.47据/td>
<td align="center">0．31据/td>
</tr>
<tr>
<td align="left">IgG2据/td>
<td align="center">1.03据/td>
<td align="center">0．17据/td>
<td align="center">0.86据/td>
<td align="center">1.47据/td>
<td align="center">30.据/td>
<td align="center">0.61据/td>
<td align="center">0.29据/td>
</tr>
<tr>
<td align="left">IgG3据/td>
<td align="center">0.77据/td>
<td align="center">0.07据/td>
<td align="center">0.71据/td>
<td align="center">0.94据/td>
<td align="center">30.据/td>
<td align="center">0．23据/td>
<td align="center">0.28据/td>
</tr>
<tr>
<td align="left">IgG4据/td>
<td align="center">1.08据/td>
<td align="center">0.03据/td>
<td align="center">1.04据/td>
<td align="center">1.14据/td>
<td align="center">30.据/td>
<td align="center">0．1据/td>
<td align="center">0.12据/td>
</tr>
<tr>
<td align="left">IgE据/td>
<td align="center">０．３据/td>
<td align="center">0.03据/td>
<td align="center">0.26据/td>
<td align="center">0.37据/td>
<td align="center">30.据/td>
<td align="center">0.11据/td>
<td align="center">0.08据/td>
</tr>
<tr class="table-tr">
<td colspan="8">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表5据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab5/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
IGG类和30天然感染的静脉患者对B抗原的平均OD值和亚类反应。据/div>
</div>
</div>
<div class="floats-partial-section partial-other-wrapper" data-section="tab6">
<div class="floats-partial-content fig-content-tab6">
<table class="table-group" id="tab6">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr class="thead">
<td align="left">OD据/td>
<td align="center">意思据/td>
<td align="center">Std.偏差据/td>
<td align="center">最低据/td>
<td align="center">最大据/td>
<td align="center">数量据/td>
<td align="center">范围据/td>
<td align="center">截止据/td>
</tr>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr>
<td align="left">免疫球蛋白据/td>
<td align="center">0.32据/td>
<td align="center">0.08据/td>
<td align="center">0.26据/td>
<td align="center">0.37据/td>
<td align="center">30.据/td>
<td align="center">０．３３据/td>
<td align="center">0.06据/td>
</tr>
<tr>
<td align="left">IgG2据/td>
<td align="center">0.26据/td>
<td align="center">0.04据/td>
<td align="center">0.21据/td>
<td align="center">0.35据/td>
<td align="center">30.据/td>
<td align="center">0．14据/td>
<td align="center">０．１３据/td>
</tr>
<tr>
<td align="left">IgG3据/td>
<td align="center">0．24据/td>
<td align="center">0．15据/td>
<td align="center">0．15据/td>
<td align="center">0.55据/td>
<td align="center">30.据/td>
<td align="center">0．4据/td>
<td align="center">0.07据/td>
</tr>
<tr>
<td align="left">IgG4据/td>
<td align="center">0．82据/td>
<td align="center">0.08据/td>
<td align="center">0.72据/td>
<td align="center">0.94据/td>
<td align="center">30.据/td>
<td align="center">0.22据/td>
<td align="center">0.08据/td>
</tr>
<tr>
<td align="left">IgE据/td>
<td align="center">０．１６据/td>
<td align="center">0.06据/td>
<td align="center">0.03据/td>
<td align="center">０．３据/td>
<td align="center">30.据/td>
<td align="center">0.28据/td>
<td align="center">0．25据/td>
</tr>
<tr class="table-tr">
<td colspan="8">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表6据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab6/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
30例自然感染hyadid患者对牛囊性粗液抗原的IgG类和亚类反应OD值的平均值。据/div>
</div>
</div>
<p>采用四种不同宿主来源(牛、鼠、羊、人)的HCFs进行ELISA，结果与所有30种人血清相似，其中IgG4对大多数抗原(小鼠HCF除外)的平均值最高，而IgG总量排在第二位。人血清对所有这些抗原均有交叉反应，对人HCF和抗原B的平均光密度(OD)值最高(见表)据a href="//www.newsama.com/journals/jpr/2012/947948/tab1/" target="_blank">1据/a>-据a href="//www.newsama.com/journals/jpr/2012/947948/tab6/" target="_blank">6.据/a>）.当对照组中对IgG类和对照的IgG类和亚类施用分析时，IgG4具有最高的平均OD值。统计上，所有抗体对这些抗原的OD值之间的差异是显着的，但所有抗原的所有抗体的OD值小于切断的OD值：因此，结果证明了阴性，并且不需要进一步分析（表格据a href="//www.newsama.com/journals/jpr/2012/947948/tab2/" target="_blank">2据/a>）.另一方面，采用ELISA法对人、小鼠、羊HCF抗原及病例组B抗原进行IgG类和亚类分析时，每个抗原的结果都与其他抗原不同。IgG3对人HCF抗原的平均OD值最低。所有人血清样本对人抗原反应的抗体OD值均值均高于临界值，因此认为所有人血清样本均为阳性，说明人抗体对动物和人HCFs的交叉反应。方差分析显示，不同来源的人抗包虫虫粗抗原抗体的平均OD值差异有统计学意义(表1)据a href="//www.newsama.com/journals/jpr/2012/947948/tab8/" target="_blank">8.据/a>） （据svg height="11.0625" id="M12" style="vertical-align:-0.30096pt;width:56.150002px;" version="1.1" viewbox="0 0 56.150002 11.0625" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
据据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>）.换句话说，人类对不同棘球蚴粗抗原的体液免疫反应是完全不同的。IgG4在其他IgG亚类中反应最高。同时，将各抗原的平均OD值除以其临界值，计算出“OD比”。虽然通过OD值比观察到对所有抗原的人IgG反应的交叉反应，但这种反应的强度是不同的，使用方差分析这种差异具有统计学意义(据svg height="11.0625" id="M13" style="vertical-align:-0.30096pt;width:56.150002px;" version="1.1" viewbox="0 0 56.150002 11.0625" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
据据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>）.ELISA检测人IgG类抗人粗HC抗原的敏感性为100%，特异性为95.8%。据/p>
<h5 id="sec3.3">３．３．人HCF ELISA结果据/h5>
<p>针对人包虫病粗液抗原，人IgG4的OD值最高(0.59)，人IgG3的OD值最低(0.16)据a href="//www.newsama.com/journals/jpr/2012/947948/tab1/" target="_blank">1据/a>）.这是抗原B后第二最高人体IgG反应（表据a href="//www.newsama.com/journals/jpr/2012/947948/tab7/" target="_blank">7.据/a>）.关于对不同HCF抗原的IgE反应，对人HCF和最低对小鼠HCF抗原观察到最高的OD值（表据a href="//www.newsama.com/journals/jpr/2012/947948/tab10/" target="_blank">10据/a>）.一般来说，人类样本血清对人源性HCF的抗体应答以IgG4、IgG和IgE最高。此外，在人抗体应答中，IgG4排在第一位，OD比为10.5,IgE排在第二位，IgG排在第三位，OD比分别为8.2和7.4(表1)据a href="//www.newsama.com/journals/jpr/2012/947948/tab11/" target="_blank">11据/a>）.据/p>
<div class="floats-partial-section partial-other-wrapper" data-section="tab7">
<div class="floats-partial-content fig-content-tab7">
<table class="table-group" id="tab7">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="8">
<hr></td>
</tr>
<tr>
<td align="left" rowspan="2">抗原来源据/td>
<td align="center" rowspan="2">OD值平均值据/td>
<td align="center" rowspan="2">Std.偏差据/td>
<td align="center" rowspan="2">Std.错误据/td>
<td align="center" colspan="2">95%的信心据/td>
<td align="center" rowspan="2">最低据/td>
<td align="center" rowspan="2">最大据/td>
</tr>
<tr>
<td align="center" colspan="2">区间的意思据/td>
</tr>
<tr>
<td align="left"></td>
<td align="center"></td>
<td align="center"></td>
<td align="center"></td>
<td align="center">下界据/td>
<td align="center">上界据/td>
<td align="center"></td>
<td align="center"></td>
</tr>
<tr>
<td align="left" colspan="8">
<hr></td>
</tr>
<tr>
<td align="left">人类据/td>
<td align="center">.5990据/td>
<td align="center">.52304据/td>
<td align="center">.04299据/td>
<td align="center">.5140据/td>
<td align="center">.6839据/td>
<td align="center">．12据/td>
<td align="center">1.99据/td>
</tr>
<tr>
<td align="left">羊据/td>
<td align="center">.3402据/td>
<td align="center">.19857据/td>
<td align="center">.01860据/td>
<td align="center">.3033据/td>
<td align="center">.3770据/td>
<td align="center">03据/td>
<td align="center">.87点据/td>
</tr>
<tr>
<td align="left">老鼠据/td>
<td align="center">.3489据/td>
<td align="center">.31243据/td>
<td align="center">.04033据/td>
<td align="center">.2682据/td>
<td align="center">.4296据/td>
<td align="center">07据/td>
<td align="center">.99据/td>
</tr>
<tr>
<td align="left">Ag) B据/td>
<td align="center">.9268据/td>
<td align="center">.40625据/td>
<td align="center">.05745据/td>
<td align="center">.8113据/td>
<td align="center">1.0422据/td>
<td align="center">点据/td>
<td align="center">1.68据/td>
</tr>
<tr>
<td align="left">牛据/td>
<td align="center">.3175据/td>
<td align="center">.22946据/td>
<td align="center">.02762据/td>
<td align="center">.2624据/td>
<td align="center">.3726据/td>
<td align="center">03据/td>
<td align="center">.94据/td>
</tr>
<tr>
<td align="left" colspan="8">
<hr></td>
</tr>
<tr>
<td align="left">全部的据/td>
<td align="center">.4912据/td>
<td align="center">.42426据/td>
<td align="center">.02020据/td>
<td align="center">.4515据/td>
<td align="center">.5309据/td>
<td align="center">03据/td>
<td align="center">1.99据/td>
</tr>
<tr class="table-tr">
<td colspan="8">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表7据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab7/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
30种自然感染人类血清对不同抗原的IgG总反应的平均值、标准差、置信区间和范围。据/div>
</div>
</div>
<div class="floats-partial-section partial-other-wrapper" data-section="tab8">
<div class="floats-partial-content fig-content-tab8">
<table class="table-group" id="tab8">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="6">
<hr></td>
</tr>
<tr class="thead">
<td align="left">OD据/td>
<td align="center">平方和据/td>
<td align="center">DF.据/td>
<td align="center">均方据/td>
<td align="center"><i>F据/i></td>
<td align="center">团体。据/td>
</tr>
<tr>
<td class="thead-hr" colspan="6">
<hr></td>
</tr>
<tr>
<td align="left">团体之间据/td>
<td align="center">17.102据/td>
<td align="center">4.据/td>
<td align="center">4.275据/td>
<td align="center">30.019据/td>
<td align="center">．01据/td>
</tr>
<tr>
<td align="left">群体内部据/td>
<td align="center">62.097据/td>
<td align="center">436据/td>
<td align="center">.142据/td>
<td align="center"></td>
<td align="center"></td>
</tr>
<tr>
<td align="left" colspan="6">
<hr></td>
</tr>
<tr>
<td align="left">全部的据/td>
<td align="center">79.199据/td>
<td align="center">440据/td>
<td align="center"></td>
<td align="center"></td>
<td align="center"></td>
</tr>
<tr class="table-tr">
<td colspan="6">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表8据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab8/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
用方差分析比较不同HCF抗原对人IgG反应的相关性。据/div>
</div>
</div>
<h5 id="sec3.4">3．4．绵羊HCF的ELISA结果据/h5>
<p>IgG4对绵羊HCF抗原的平均均值最高，而IgG2最低（表据a href="//www.newsama.com/journals/jpr/2012/947948/tab10/" target="_blank">10据/a>）.人IgG类和亚类对绵羊HCF抗原反应性高，所有抗体的OD值均在临界值以上。人抗体对绵羊HCF的反应模式与人HCF相似，因此与人抗体对这两种抗原源的交叉反应相似。对人HCF的抗体反应明显强于绵羊HCF。为了分析抗体的反应强度，计算了所有抗体的OD值。IgG4占总IgG的比例居首位，IgG总IgG占总IgG的比例居第二位据a href="//www.newsama.com/journals/jpr/2012/947948/tab11/" target="_blank">11据/a>）.方差分析显示，抗体对该抗原反应的平均OD值有显著差异(据svg height="11.0625" id="M14" style="vertical-align:-0.30096pt;width:56.150002px;" version="1.1" viewbox="0 0 56.150002 11.0625" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
据据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>）.据/p>
<h5 id="sec3.5">3．5．小鼠HCF的ELISA结果据/h5>
<p>对于这种抗原，人IgG的平均OD值更高，而人IgG4的OD值更高(表)据a href="//www.newsama.com/journals/jpr/2012/947948/tab10/" target="_blank">10据/a>-据a href="//www.newsama.com/journals/jpr/2012/947948/tab11/" target="_blank">11据/a>）.换句话说，在ELISA中，人IgG4和IgG分别是小鼠HCF反应最强烈的抗体，其模式与人和羊HCF相似。据/p>
<h5 id="sec3.6">3.6。牛HCF的ELISA结果据/h5>
<p>人抗牛HCF抗体的OD值较高，IgG4抗体和IgG抗体的OD值(OD比)也较强。据/p>
<h5 id="sec3.7">3.7。B抗原ELISA结果据/h5>
<p>人抗体对B抗原的反应最强的是IgG4和IgG2，其他抗体的反应也较高。值得说的是,模式类似于上面提到的抗原显示人类的血清可以与人类和动物的HCF抗原反应在类似的方式对一些动物即使有更强的反应抗原牢记本研究的假说,对不同的抗原交叉反应的抗体。据/p>
<h5 id="sec3.8">3.8。十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)结果据/h5>
<p>在本研究中，几乎所有的抗原在SDS - PAGE上都有相似的抗原片段。这种相似性在人类、老鼠和牛身上更为显著。据/p>
<h5 id="sec3.9">3.9。不同的HCFs对人IgG类的反应据/h5>
<p>对人HCF抗原与其他动物(小鼠、绵羊、牛和抗原B)的抗原进行方差分析和后期分析，发现对人IgG类反应的平均OD值有显著差异(据svg height="11.0625" id="M15" style="vertical-align:-0.30096pt;width:56.150002px;" version="1.1" viewbox="0 0 56.150002 11.0625" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
据据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>)(表据a href="//www.newsama.com/journals/jpr/2012/947948/tab9/" target="_blank">9.据/a>）.对羊的HCFs和其他HCFs也进行了相同的分析(表)据a href="//www.newsama.com/journals/jpr/2012/947948/tab10/" target="_blank">10据/a>)，导致绵羊HCF与人HCF的平均OD值有显著差异(据svg height="11.0625" id="M16" style="vertical-align:-0.30096pt;width:56.150002px;" version="1.1" viewbox="0 0 56.150002 11.0625" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
据据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>)，而绵羊抗原与牛抗原在小鼠体内的平均OD值差异无统计学意义。据/p>
<div class="floats-partial-section partial-other-wrapper" data-section="tab9">
<div class="floats-partial-content fig-content-tab9">
<table class="table-group" id="tab9">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="7">
<hr></td>
</tr>
<tr>
<td align="left">（i）ag据/td>
<td align="center">（j）ag据/td>
<td align="center">平均差(i j)据/td>
<td align="center">Std.错误据/td>
<td align="center">团体。据/td>
<td align="center" colspan="2">95％置信区间据/td>
</tr>
<tr>
<td align="left"></td>
<td align="center"></td>
<td align="center"></td>
<td align="center"></td>
<td align="center"></td>
<td align="center">下界据/td>
<td align="center">上界据/td>
</tr>
<tr>
<td align="left" colspan="7">
<hr></td>
</tr>
<tr>
<td align="left" rowspan="4">人类据/td>
<td align="center">羊据/td>
<td align="center">.25878 *据/td>
<td align="center">.04703据/td>
<td align="center">组织据/td>
<td align="center">.1663据/td>
<td align="center">.3512据/td>
</tr>
<tr>
<td align="center">老鼠据/td>
<td align="center">.25007 *据/td>
<td align="center">.05776据/td>
<td align="center">组织据/td>
<td align="center">.1365据/td>
<td align="center">.3636据/td>
</tr>
<tr>
<td align="center">B.据/td>
<td align="center">-.32781 *据/td>
<td align="center">.06173据/td>
<td align="center">组织据/td>
<td align="center">−.4491据/td>
<td align="center">−.2065据/td>
</tr>
<tr>
<td align="center">牛据/td>
<td align="center">.28147 *据/td>
<td align="center">.05501据/td>
<td align="center">组织据/td>
<td align="center">.1734据/td>
<td align="center">.3896据/td>
</tr>
<tr>
<td align="left" colspan="7">
<hr></td>
</tr>
<tr>
<td align="left" rowspan="4">羊据/td>
<td align="center">人类据/td>
<td align="center">−.25878 *据/td>
<td align="center">.04703据/td>
<td align="center">组织据/td>
<td align="center">-.3512据/td>
<td align="center">-.1663据/td>
</tr>
<tr>
<td align="center">老鼠据/td>
<td align="center">−.00871据/td>
<td align="center">.06019据/td>
<td align="center">.885据/td>
<td align="center">−.1270据/td>
<td align="center">.1096据/td>
</tr>
<tr>
<td align="center">B.据/td>
<td align="center">−.58658 *据/td>
<td align="center">.06401据/td>
<td align="center">组织据/td>
<td align="center">−.7124据/td>
<td align="center">-.4608据/td>
</tr>
<tr>
<td align="center">牛据/td>
<td align="center">.02270据/td>
<td align="center">.05756据/td>
<td align="center">.694据/td>
<td align="center">−.0904据/td>
<td align="center">.1358据/td>
</tr>
<tr>
<td align="left" colspan="7">
<hr></td>
</tr>
<tr>
<td align="left" rowspan="4">老鼠据/td>
<td align="center">人类据/td>
<td align="center">−.25007 *据/td>
<td align="center">.05776据/td>
<td align="center">组织据/td>
<td align="center">-.3636据/td>
<td align="center">−.1365据/td>
</tr>
<tr>
<td align="center">羊据/td>
<td align="center">.00871据/td>
<td align="center">.06019据/td>
<td align="center">.885据/td>
<td align="center">−.1096据/td>
<td align="center">.1270据/td>
</tr>
<tr>
<td align="center">B.据/td>
<td align="center">−.57788 *据/td>
<td align="center">.07227据/td>
<td align="center">组织据/td>
<td align="center">-.7199据/td>
<td align="center">−.4358据/td>
</tr>
<tr>
<td align="center">牛据/td>
<td align="center">.03141据/td>
<td align="center">.06662据/td>
<td align="center">.638据/td>
<td align="center">−.0995据/td>
<td align="center">.1623据/td>
</tr>
<tr>
<td align="left" colspan="7">
<hr></td>
</tr>
<tr>
<td align="left" rowspan="4">B.据/td>
<td align="center">人类据/td>
<td align="center">.32781 *据/td>
<td align="center">.06173据/td>
<td align="center">组织据/td>
<td align="center">.2065据/td>
<td align="center">.4491据/td>
</tr>
<tr>
<td align="center">羊据/td>
<td align="center">.58658 *据/td>
<td align="center">.06401据/td>
<td align="center">组织据/td>
<td align="center">.4608据/td>
<td align="center">.7124据/td>
</tr>
<tr>
<td align="center">老鼠据/td>
<td align="center">.57788 *据/td>
<td align="center">.07227据/td>
<td align="center">组织据/td>
<td align="center">.4358据/td>
<td align="center">.7199据/td>
</tr>
<tr>
<td align="center">牛据/td>
<td align="center">.60928 *据/td>
<td align="center">.07009据/td>
<td align="center">组织据/td>
<td align="center">.4715据/td>
<td align="center">.7470据/td>
</tr>
<tr>
<td align="left" colspan="7">
<hr></td>
</tr>
<tr>
<td align="left" rowspan="4">牛据/td>
<td align="center">人类据/td>
<td align="center">−.28147 *据/td>
<td align="center">.05501据/td>
<td align="center">组织据/td>
<td align="center">-.3896据/td>
<td align="center">−.1734据/td>
</tr>
<tr>
<td align="center">羊据/td>
<td align="center">−.02270据/td>
<td align="center">.05756据/td>
<td align="center">.694据/td>
<td align="center">−.1358据/td>
<td align="center">.0904据/td>
</tr>
<tr>
<td align="center">老鼠据/td>
<td align="center">-.03141.据/td>
<td align="center">.06662据/td>
<td align="center">.638据/td>
<td align="center">−.1623据/td>
<td align="center">.0995据/td>
</tr>
<tr>
<td align="center">B.据/td>
<td align="center">−.60928 *据/td>
<td align="center">.07009据/td>
<td align="center">组织据/td>
<td align="center">−.7470据/td>
<td align="center">-.4715据/td>
</tr>
<tr class="table-tr">
<td colspan="7">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
<tr class="table-fn">
<td>*平均差异在0.05水平上显著。据br></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表9据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab9/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
基于人总IgG反应的平均OD值采用事后分析对不同HCF抗原进行多变量分析。据/div>
</div>
</div>
<div class="floats-partial-section partial-other-wrapper" data-section="tab10">
<div class="floats-partial-content fig-content-tab10">
<table class="table-group" id="tab10">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="11">
<hr></td>
</tr>
<tr class="thead">
<td align="left"></td>
<td align="center">牛的意思是OD据/td>
<td align="center">截止据/td>
<td align="center">Ag B平均OD值据/td>
<td align="center">截止据/td>
<td align="center">老鼠的意思是OD据/td>
<td align="center">截止据/td>
<td align="center">绵羊意味着杂志据/td>
<td align="center">截止据/td>
<td align="center">人类的意思od.据/td>
<td align="center">截止据/td>
</tr>
<tr>
<td class="thead-hr" colspan="11">
<hr></td>
</tr>
<tr>
<td align="left">免疫球蛋白据/td>
<td align="center">0.32据/td>
<td align="center">0.06据/td>
<td align="center">0.93据/td>
<td align="center">0．31据/td>
<td align="center">0.35据/td>
<td align="center">0.06据/td>
<td align="center">0.34据/td>
<td align="center">0.12据/td>
<td align="center">０．５９据/td>
<td align="center">0.08据/td>
</tr>
<tr>
<td align="left">IgG2据/td>
<td align="center">0.26据/td>
<td align="center">０．１３据/td>
<td align="center">1.03据/td>
<td align="center">0.29据/td>
<td align="center">0．14据/td>
<td align="center">0.12据/td>
<td align="center">0.21据/td>
<td align="center">0.08据/td>
<td align="center">0.34据/td>
<td align="center">0.12据/td>
</tr>
<tr>
<td align="left">IgG3据/td>
<td align="center">0．24据/td>
<td align="center">0.07据/td>
<td align="center">0.77据/td>
<td align="center">0.28据/td>
<td align="center">0.12据/td>
<td align="center">０．１６据/td>
<td align="center">0．25据/td>
<td align="center">0.11据/td>
<td align="center">０．１６据/td>
<td align="center">0.11据/td>
</tr>
<tr>
<td align="left">IgG4据/td>
<td align="center">0．82据/td>
<td align="center">0.08据/td>
<td align="center">1.08据/td>
<td align="center">0.12据/td>
<td align="center">0.29据/td>
<td align="center">0.04据/td>
<td align="center">0.70据/td>
<td align="center">0．14据/td>
<td align="center">1.58据/td>
<td align="center">0．15据/td>
</tr>
<tr>
<td align="left">IgE据/td>
<td align="center">０．１６据/td>
<td align="center">0．25据/td>
<td align="center">０．３据/td>
<td align="center">0.08据/td>
<td align="center">0.12据/td>
<td align="center">0.11据/td>
<td align="center">0．31据/td>
<td align="center">0.12据/td>
<td align="center">0.49据/td>
<td align="center">0.06据/td>
</tr>
<tr class="table-tr">
<td colspan="11">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
<tr class="table-fn">
<td>
<svg height="11.0625" id="M17" style="vertical-align:-0.30096pt" version="1.1" viewbox="0 0 56.150002 11.0625" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
据据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>。据br></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表10据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab10/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
平均OD值IgG类，IgG亚类和30个人样品血清的IgE，牛，Ag，小鼠，绵羊和人的粗HCF抗原。据/div>
</div>
</div>
<div class="floats-partial-section partial-other-wrapper" data-section="tab11">
<div class="floats-partial-content fig-content-tab11">
<table class="table-group" id="tab11">
<tbody>
<tr>
<td>
<table class="table">
<tbody>
<tr>
<td class="thead-hr" colspan="6">
<hr></td>
</tr>
<tr class="thead">
<td align="left">IG / AG起源据/td>
<td align="center">牛据/td>
<td align="center">Ag) B据/td>
<td align="center">老鼠据/td>
<td align="center">羊据/td>
<td align="center">人类据/td>
</tr>
<tr>
<td class="thead-hr" colspan="6">
<hr></td>
</tr>
<tr>
<td align="left">免疫球蛋白据/td>
<td align="center">5．3据/td>
<td align="center">3.据/td>
<td align="center">5．8据/td>
<td align="center">2.8.据/td>
<td align="center">7.4据/td>
</tr>
<tr>
<td align="left">IgG2据/td>
<td align="center">2据/td>
<td align="center">4．5据/td>
<td align="center">1．2据/td>
<td align="center">2.6.据/td>
<td align="center">2.8.据/td>
</tr>
<tr>
<td align="left">IgG3据/td>
<td align="center">3.4.据/td>
<td align="center">2.75据/td>
<td align="center">０．７５据/td>
<td align="center">2.3.据/td>
<td align="center">1．4据/td>
</tr>
<tr>
<td align="left">IgG4据/td>
<td align="center">10.2据/td>
<td align="center">9.据/td>
<td align="center">7.25据/td>
<td align="center">5.据/td>
<td align="center">10.5据/td>
</tr>
<tr>
<td align="left">IgE据/td>
<td align="center">0.64据/td>
<td align="center">3．75据/td>
<td align="center">1．1据/td>
<td align="center">2.6.据/td>
<td align="center">8.2据/td>
</tr>
<tr class="table-tr">
<td colspan="6">
<hr class="tbody-hr"></td>
</tr>
</tbody>
</table></td>
</tr>
<tr class="table-fn">
<td>OD比值计算为每个抗原的平均OD值除以该抗原的切点。据br></td>
</tr>
</tbody>
</table>
</div>
<div class="floats-partial-footer">
<div class="floats-partial-caption">
<span class="caption-text">表11据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/tab11/" title="" target="_blank"></a>
</div>
<div class="floats-partial-caption-text">
30份人血清IgG类、IgG亚类和IgE对牛、Ag B、小鼠、绵羊和人HCF粗抗原的OD值比。据/div>
</div>
</div>
<p>最后，对小鼠HCF抗原与其他资源的差异均值OD进行分析时，绵羊和牛的小鼠HCF差异不显著，而与人的小鼠HCF差异显著(据svg height="11.0625" id="M18" style="vertical-align:-0.30096pt;width:56.150002px;" version="1.1" viewbox="0 0 56.150002 11.0625" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
据据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>）.据/p>
<h4 id="discussion-and-conclusion">4.讨论与结论据/h4>
<p>IgG4子类在其他人HCF中的其他人IGG子类中的最高平均值值与Siracusano等人进行的研究结果一致。[据a href="#B19">15据/a>]，Khabiri等。[据a href="#B20">16据/a>[文和克雷格[据a href="#B21">17据/a>]，Dreweck等。[据a href="#B22">18据/a>，和Grimm等[据a href="#B23">19据/a>］.人IgG4对绵羊和小鼠HCF的平均OD值也高于其他IgG亚类。这表明IgG类和IgG4亚类对HCF抗原的反应最高。我们的研究表明，该IgG亚类对牛HCF抗原和抗原B的平均OD值也最高，这与Siracusano等人的研究结果一致[据a href="#B19">15据/a>文和克雷格[据a href="#B21">17据/a>］.如前所述，当前研究的目的是找到对所有抗原都有反应的最佳抗体，同时也是对人血清抗体反应性最强的抗原。换句话说，在人、牛、鼠、羊中寻找对人抗体有最佳交叉反应的抗原是本研究的目标。从获得的平均OD值来看，人类HCF和抗原B似乎在HCF抗原的顶端。为了更好的分析，我们计算每个抗原的平均OD值与临界值的比值，因为每个抗原的阳性通常都是基于这个比值。这一比例主要是针对人IgG4对抗每种抗原而获得的。人IgG4与人HCF的比值为10.5。也就是说，人IgG4对人HCF的平均OD值是其临界值的10.5倍。其后依次为牛(10.2)、B抗原(9)、小鼠(7.25)。IgG对不同抗原的反应比率有显著差异(据svg height="11.0625" id="M19" style="vertical-align:-0.30096pt;width:56.150002px;" version="1.1" viewbox="0 0 56.150002 11.0625" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
据据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>）.作为结论，可以说，人HCF，抗原B和牛HCF通过使用人IgG4作为最佳IgG亚类响应，具有最佳的交叉反应。从人IgG类和IgG亚类分析的结果中也可以看出，HCF抗原之间的差异有统计学意义（据svg height="11.0625" id="M20" style="vertical-align:-0.30096pt;width:56.150002px;" version="1.1" viewbox="0 0 56.150002 11.0625" width="56.150002" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0625)">
<g transform="translate(72,-63.15)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">
据/tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">
据据/tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="29.182001" y="0">
。据/tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">
0.据/tspan>
<tspan style="font-size: 12.50px; " x="38.559254" y="0">
1据/tspan>
</text>
</g>
</g>
</svg>使用方差分析)。这些数据表明，某些抗原在提高人类血清反应方面强于其他抗原，某些IgG亚类在诊断不同来源的HCF抗原方面也优于其他IgG亚类，这已被Sbihi等人证实[据a href="#B24">20.据/a>和Grimm等[据a href="#B23">19据/a>]。虽然IgG反应的最高比例在人类HCF中较高，但在小鼠HCF中也有相当大的比例。小鼠HCF检测简便，可有效用于包虫病的诊断。据/p>
<p>一般来说，采用人IgG类进行ELISA时，抗B抗原的平均OD值最高，其次是人HCF(图)据a href="//www.newsama.com/journals/jpr/2012/947948/fig2/" target="_blank">2据/a>）.人源HCF抗原与动物源HCF抗原之间存在强烈的交叉反应，其中人源HCF抗原和小鼠源HCF抗原的交叉反应最为显著。据/p>
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<span class="caption-text">图2据/span>
<a aria-label="full view" class="caption-partial-url" href="//www.newsama.com/journals/jpr/2012/947948/fig2/" title="" target="_blank"></a>
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<div class="floats-partial-caption-text">
<table>
对小鼠，牛，人和绵羊HCF与抗原B对小鼠，牛，人和绵羊HCF的总体IgG反应的平均异常值。据/table>
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<h4 id="limitations">5.限制据/h4>
<p>在临床实践中检测特异性血清抗体的试验在CE的诊断中特别重要，而循环抗原的检测较低。即使使用高敏感的测试，例如IgG-ELISA，抗体可能在一定比例的呼吸功能亢进患者（假阴性结果）中可能无法检测到。脑或眼睛和钙化囊肿中的囊肿通常诱导低或没有抗体滴度。抗体反应也可能低于某些人口群体和幼儿。还可能发生假阳性结果，尤其是患有其他蠕虫疾病的患者[据a href="#B25">21据/a>］.据/p>
<h4 id="acknowledgment">承认据/h4>
<p>本文作者均同意本文的内容，并对本研究作出贡献。伊拉姆医科大学和伊拉姆大学为实验室研究和解释提供了部分支助。据/p>
</div>
<div class="xml-content">
<h4 class="references" id="references">参考据/h4>
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</ol>
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<h4 class="copyright" id="copyright">版权据/h4>
<p>版权所有©2012 Afra Khosravi等人。这是一篇发布在据a rel="license" href="http://creativecommons.org/licenses/by/3.0/">创意公共归因许可证据/a>，允许在任何媒介上不受限制地使用、传播和复制，但必须正确引用原作。据/p>
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<a class="sc-htpNat bUhGXt link sc-jtRfpW dIjoCg sc-exkUMo hymroZ pdf_desktop button__group" href="https://downloads.hindawi.com/journals/jpr/2012/947948.pdf" aria-label="" target="_blank" id="toolbar__dcNavSmall"><span><img alt="" class="sc-EHOje jOLhQl sc-kTUwUJ eujois" title="" role="presentation" src="https://static-01.hindawi.com/next_assets/Po4bqLHwKXT_IKpRPJlxK/_next/static/node_modules/hindawi-ui/src/icon/svg/pdf.svg" height="24"><span class="text">PDF据/span></span><span class="sc-htoDjs bqeMOy sc-dqBHgY cQgXqt fileArrow icon-colored _3P4iUn1NDM5G77nWCamJ4l icon-themeable" title="" font-size="20"></span></a>
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的观点据span>1858据/span>
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下载据span>830.据/span>
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<span class="__dimensions_badge_embed__" data-doi="10.1155/2012/947948" data-style="small_circle"></span>引用据/div>
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