TY - JOUR A2 - Ortega-Pacheco, Antonio AU - Aksono, Eduardus Bimo AU - Riwu, Katty Hendriana Priscilla AU - Estoepangestie, A. T. Soelih AU - Pertiwi, Herinda PY - 2020 DA - 2020/03/01 TI - Phylogenetic Analysis and Antibiotics Resistance of李斯特菌污染鸡肉在印尼泗水SP - 9761812 VL - 2020 AB - 本研究的目的是确定的系统发育分析和耐药性
李斯特菌污染在泗水鸡肉。60个鸡肉样本来自超市,流动摊贩,并在泗水传统市场收集。选择性培养基用于分离和鉴定
李斯特菌通过切碎将25克的鸡肉,并把它放入灭菌锥形瓶中。被用于识别程序,如生物化学和形态学测试,抗生素抗性测试,PCR和测序的一些方法;还一个系统发育分析通过使用GENETIX的Mac版本8.0的靶向hlyA基因的邻接分析进行
李斯特菌记录在GenBank中,如谱系I(KC808543),天堂II(AY229462,AY229346,AY229499,AY229404和),谱系III(KJ504139,HQ686043,KJ504116,和DQ988349)和谱系IV(EU840690,EF030606)。结果表明,患病率
单增李斯特菌在泗水污染从超市鸡肉的样品为10%(2/20),从移动供应商为0/20(0%),以及从传统市场为5%(1/20)。It was seen from the band at 456 bp fragment. Furthermore, three isolates found in Surabaya were included in the new lineages which were resistant to old-generation antibiotics such as sulfamethonazole-trimetophrim (SXT) and amoxyllin sulbactam (MAS), but they were still sensitive to new-generation antibiotics such as cefotaxime (CTX) and meropenem (MEM). SN - 2090-8113 UR - https://doi.org/10.1155/2020/9761812 DO - 10.1155/2020/9761812 JF - Veterinary Medicine International PB - Hindawi KW - ER -