ty -jour a2 -ogasawara，toru au -pieles，oliver au -höring，Marcus au -Adel，Sadiyeh au -Reichert，Torsten E. Au -Liebisch，Gerhard Au -Morsczeck，Mororsczeck，Christian PY -2022 DA -2022 DA -2022 DA -2022 DA -2022/07/16/16/16/16Ti-能量代谢和脂肪组在牙齿卵泡细胞的成骨分化过程中受到高度调节-3674931 VL -2022 AB -2022 AB-牙齿卵泡细胞（DFC）是牙周的茎/祖细胞，并引起肺泡的骨质成骨细胞。然而，对基于细胞疗法所需的成骨分化的分子机制的理解是界定的。这项研究旨在分析DFC的成骨分化过程中的能量代谢。培养人类DFC，并通过地塞米松或骨形态发生蛋白2（BMP2）诱导成骨分化。将先前的微阵列数据重新分析以检查成骨诱导后受调节的途径。通过蛋白质印迹分析和特定测定评估了代谢标记的表达和活性，通过实时定量聚合酶链反应，通过谷胱甘肽测定法确定细胞的氧化态，线粒体DNA的相对量测量，并通过细胞的脂质组确定。通过质谱法（MS）分析。此外，在5-（四氧基）-2-氟酸或C75抑制脂肪酸合成后，分析了成骨标记。微阵列数据的途径富集分析表明，DFC中成骨诱导后，碳代谢是最受监管的途径之一。 Further analysis showed that enzymes involved in glycolysis, citric acid cycle, mitochondrial activity, and lipid metabolism are differentially expressed during differentiation, with most markers upregulated and more markedly after induction with dexamethasone compared to BMP2. Moreover, the cellular state was more oxidized, and mitochondrial DNA was distinctly upregulated during the second half of differentiation. Besides, MS of the lipidome revealed higher lipid concentrations after osteogenic induction, with a preference for species with lower numbers of C-atoms and double bonds, which indicates a de novo synthesis of lipids. Concordantly, inhibition of fatty acid synthesis impeded the osteogenic differentiation of DFCs. This study demonstrates that energy metabolism is highly regulated during osteogenic differentiation of DFCs including changes in the lipidome suggesting enhanced de novo synthesis of lipids, which are required for the differentiation process. SN - 1687-966X UR - https://doi.org/10.1155/2022/3674931 DO - 10.1155/2022/3674931 JF - Stem Cells International PB - Hindawi KW - ER -