TY -的A2 Gan Yibo盟——曾永胜AU - Du,成成非盟-肖,Pengcheng AU - Lei,日前盟——赵,朴盟——朱Zhenglin AU -高,Shengqiang盟——陈,鲍文AU - Cheng Shengwen盟——黄、陈魏盟——赵、PY - 2021 DA - 2021/11/05 TI - Sox9-Increased mir - 322 - 5 - p促进BMP2-Induced Chondrogenic针对Smad7分化的间充质干细胞SP - 9778207六世- 2021 AB -骨形成蛋白2 (BMP2)诱导软骨形成有效促进间充质干细胞(msc)的Sox9表达式。然而,BMP2也诱导软骨细胞肥大和软骨内成骨移植Smad7表达,导致软骨形成的破坏。此外,可以抑制Smad7 Sox9。因此,这种现象的潜在机制仍不清楚。目前,越来越多的研究表明,小分子核糖核酸chondrogenic和病理生理过程中发挥关键作用的软骨。本研究的目的是确定哪些Smad7 microRNA是增加了Sox9,目标,从而协助BMP2维持稳定的软骨形成。我们发现mir - 322 - 5 - p满足要求通过下一代测序(上天)和生物信息学分析。针对关系mir - 322 - 5 - p和Smad7 dual-luciferase记者化验,证实了qPCR,免疫印迹(WB)。体外研究表明,过度的mir - 322 - 5 - p明显抑制Smad7表达式,从而导致增加chondrogenic分化和减少肥厚性分化,而沉默mir - 322 - 5 - p导致相反的结果。流式细胞仪(FCM)分析表明,过度的mir - 322 - 5 - p明显降低早期凋亡率BMP2-stimulated msc、虽然沉默mir - 322 - 5 - p率增加。 A mouse limb explant assay revealed that the expression of miR-322-5p was negatively correlated with the length of the BMP2-stimulated hypertrophic zone of the growth plate. An in vivo study also confirmed that miR-322-5p assisted BMP2 in chondrogenic differentiation. Taken together, our results suggested that Sox9-increased miR-322-5p expression can promote BMP2-induced chondrogenesis by targeting Smad7, which can be exploited for effective tissue engineering of cartilage. SN - 1687-966X UR - https://doi.org/10.1155/2021/9778207 DO - 10.1155/2021/9778207 JF - Stem Cells International PB - Hindawi KW - ER -