TY - JOUR A2 - 彼得连科，尤里AU - 钟浩AU - 沉亚美AU - 赵，丹辉AU - 燕国强AU - 吴，Chengai AU - 黄，王光林AU - 刘治中AU - 斋，Jianpo AU -韩，秦PY - 2021 DA - 2021年6月7日TI - 细胞种植脱细胞动脉在犬模型SP龙尿道缺损的修复 - 8854479 VL - 2021 AB - 尿道狭窄的管理仍处于诊所主要治疗难题。在此，我们探索了在犬模型的细胞接种无细胞动脉重建尿道的相对长段的可行性。从供体犬切除颈动脉获得的脱细胞基质的动脉。从6只雄性犬自体脂肪来源干细胞（ADSC中）中生长，并接种到预制无细胞动脉基质。A 3 cm long segment of the urethra was resected in 12 male dogs. Urethroplasty was performed with the acellular arterial matrix seeded with ADSCs in 6 animals and without cells in 6. Serial urethrography was performed at 1 and 3 months postoperatively. Wide urethral calibers without any signs of strictures were confirmed in all 6 animals in the experimental group. In contrast, urethral stricture was demonstrated in 3 animals in the control group. The graft was highly epithelialized and smooth in the experimental group, while graft contracture and scar formation were showed in the control group. Histologic analysis of the cell-seeded arterial matrix at 1 month confirmed the presence of multilayered urothelium and muscle. The levels of tissue formation developed over time with a progressive increase in muscle content. In contrast, extensive fibrosis and sparse smooth muscle were seen in animals treated with matrix without ADSCs. This study provides preclinical evidence that the ADSC-seeded arterial matrix can be used as a tubularized scaffold in the reconstruction of 3 cm long urethral defect in a male canine model. The ADSC-seeded arterial matrix remodels and regenerates normal-appearing urethral tissue layers over time. SN - 1687-966X UR - https://doi.org/10.1155/2021/8854479 DO - 10.1155/2021/8854479 JF - Stem Cells International PB - Hindawi KW - ER -