TY -的A2 Rodr guez Lozano, Francisco j . AU -翟,启明创投盟——咚,益盟——张Xuesi盟——他,Xiaoning盟——范东东AU -金,燕盟-李,贝盟金,方PY - 2021 DA - 2021/01/11 TI -间充质干细胞提高疗效,防止胃肠道不良反应的甲氨蝶呤治疗胶原诱导关节炎SP - 8850820六世- 2021 AB类风湿性关节炎(RA)是一种系统性自身免疫性疾病,其特征是关节破坏和功能丧失。氨甲叶酸(简称MTX)为甲酰在RA治疗有效。然而,MTX诱发一些不良事件和20% - -30%的病人不响应MTX。因此,迫切需要提高治疗效果,减少MTX的副作用。最近的研究表明,间充质干细胞(msc)是参与抗炎、免疫调节、组织再生。然而,msc的结合应用和MTX是否促进了治疗效果和减少MTX的副作用还没有被研究过。在这项研究中,我们使用牛II型胶原诱导类风湿关节炎小鼠(胶原诱导关节炎,中情局)。然后,CIA小鼠受到MTX或MSC治疗,或两者兼而有之。不同治疗方法的疗效和不良事件在RA与ct机进行评估,他体内,免疫组织化学染色。MODE-K细胞的凋亡和增殖后测定MTX或/和cocultured UCs对待。 To test M2 polarization, Raw264.7 macrophages were stimulated by MTX with different concentrations or cocultured with UCs. We found that the combined application of MSCs and MTX increased the therapeutic effects on RA, as evidenced by decreased arthritis score, inflammatory responses, and mortality. Moreover, in this combination remedy, MTX prefers to suppress inflammation by facilitating macrophage polarization to M2 type while UCs prefer to eliminate gastrointestinal side effects of MTX via mitigating the apoptosis of intestinal epithelial cells. Thus, a combination of MTX and UCs is a promising strategy for RA treatment. SN - 1687-966X UR - https://doi.org/10.1155/2021/8850820 DO - 10.1155/2021/8850820 JF - Stem Cells International PB - Hindawi KW - ER -