TY -的A2 - De Francesco Francesco盟——周Shukui AU -王,应非盟-张,恺乐盟——曹Nailong AU -杨,Ranxing盟——黄Jianwen盟——赵Weixin AU -拉赫曼Mahbubur盟——廖,香港非盟- Fu,羌族PY - 2020 DA - 2020/02/10 TI -一个潜在的生物材料的制备和评价干细胞表技术对未来再生医学SP - 9567362六世- 2020 AB -迄今为止,脱细胞支架作为再生医学的生物支架已得到广泛的研究。然而，来源于天然组织器官的脱细胞基质存在很多缺陷，包括自体组织数量有限，以及手术并发症，如失血、伤口感染、疼痛、休克、供体部分功能损害等风险。在本研究中，我们使用脂肪来源干细胞(ADSC)薄片制备脱细胞基质，并评估细胞相容性和免疫反应性。制作ADSC薄片，随后使用反复冻融、Triton X-100和SDS脱细胞。将口腔粘膜上皮细胞接种到去细胞化的ADSC薄片上，观察细胞再植能力，以丝素蛋白作为对照。脱细胞基质植入皮下组织1周或3周;采用H&E染色和免疫组化方法分析CD68的表达，采用实时荧光定量PCR (qPCR)方法评价其免疫原性和生物相容性。ADSC平板衍生ECM支架通过Triton X-100脱细胞方案保留了ECM的三维结构并保留了细胞因子。口腔黏膜上皮细胞在脱细胞的ADSC薄片上存活较好，表皮细胞层完整、连续。 Compared with porcine small intestinal submucosa (SIS) in vivo, the homogeneous decellularized ADSC sheets had less monocyte-macrophage infiltrating in vivo implantation. During 3 weeks after transplantation, the mRNA expression of cytokines, such as IL-4/IL-10, was obviously higher in decellularized ADSC sheets than that of porcine SIS. A Triton X-100 method can achieve effective cell removal, retain major ECM components, and preserve the ultrastructure of ADSC sheets. The decellularized ADSC sheets possess good recellularization capacity and excellent biocompatibility. This study demonstrated the potential suitability of utilizing acellular matrix from ADSC sheets for soft tissue regeneration and repair. SN - 1687-966X UR - https://doi.org/10.1155/2020/9567362 DO - 10.1155/2020/9567362 JF - Stem Cells International PB - Hindawi KW - ER -