TY -的A2 Zimmerlin卢多维奇盟,岸本聪盟——井上,中国云南盟——Sohma,君非盟-丰田,茂盟——佐Masashi盟——井上Teruo盟,吉田Ken-ichiro PY - 2020 DA - 2020/05/18 TI -手术损伤和缺血'脂肪间质血管分数和增加血管生成能力在鼠标肢体缺血模型SP - 7219149六世- 2020 AB -脂肪基质血管分数(SVF)是一种有效的自体移植细胞来源。然而，SVFs的质量和数量取决于患者的年龄、并发症和其他因素。在这项研究中，我们开发了一种通过外科手术来重复增加脂肪源性SVFs细胞数量和提高其质量的方法，我们称之为“伤口修复启动”。来自BALB/c小鼠腹股沟区的皮下脂肪被外科处理(启动)，通过切碎脂肪实质(损伤)和结扎皮下脂肪供给动脉(缺血)。在启动后的第0、1、3、5、7天分离SVFs。通过微阵列和通路分析(对差异表达的基因进行分析)测定引物SVFs的基因表达水平。流式细胞仪检测引物SVFs细胞组成变化。将SVFs移植到同基因缺血后肢，观察其血管生成和再生潜力。采用激光多普勒血流灌注成像仪测量后肢血流，用CD31染色对缺血组织进行毛细管密度量化。分别通过荧光免疫染色和Western blotting检测SVFs中HIF-1和VEGF-A合成的稳定性。 As a result, the number of SVFs per fat weight was increased significantly on day 7 after priming. Among the differentially expressed genes were innate immunity-related signals on both days 1 and 3 after priming. In primed SVFs, the CD45-positive blood mononuclear cell fraction decreased, and the CD31-CD45-double negative mesenchymal cell fraction increased on day 7. The F4/80-positive macrophage fraction was increased on days 1 and 7 after priming. There was a serial decrease in the mesenchymal-gated CD34-positive adipose progenitor fraction and mesenchymal-gated CD140A-positive/CD9-positive preadipocyte fraction on days 1 and 3. Transplantation of primed SVFs resulted in increased capillary density and augmented blood flow, improving regeneration of the ischemic limbs. HIF-1 alpha was stabilized in the primed cutaneous fat 原位，而引物SVFs的VEGF-A合成在引物后5天达到高峰。创面修复启动导致SVFs数量增加，血管生成潜能增强。SN - 1687-966X UR - https://doi.org/10.1155/2020/7219149 DO - 10.1155/2020/7219149 JF -国际干细胞组织PB - Hindawi KW - ER -