TY - JOUR A2 - Najimi，穆斯塔法AU - BA，瑞凯AU - 香港，梁盟 - 吴，国丰AU - 刘士余AU - 董燕AU - 李，北盟 - 赵，益民PY - 2020 DA - 2020 /05/26 TI - 在血小板富集血浆增加骨髓基质细胞/软骨细胞砖余额中Sox9基因的表达促进稳定的3-d软骨微环境对骨髓基质干细胞的SP的构建 - 5492059 VL - 2020 AB - Sox9的是与本征转录因子判定和骨髓的软骨细胞系的维持间充质干细胞（BMSC）。在最近的研究中，我们已经证明了软骨碎片聚集体（细胞砖）可促进体内骨髓基质干细胞的软骨。但是，它仍是未知的BMSCs /软骨细胞砖的比率是否对3-d软骨再生和相关分子机制影响显著。To address this issue, the current study subcutaneously injected three groups of cell complex with different rabbit BMSCs/chondrocyte bricks’ ratios (1 : 2, 1 : 1, and 2 : 1) into nude mice. Gross morphology observation, histological and immunohistochemical assays, biochemical analysis, gene expression analysis, and western blot were used to compare the influence of different BMSCs/chondrocyte bricks’ ratios on the properties of tissue-engineered cartilage and explore the related molecular mechanism. The constructs of 1 : 1 BMSCs/chondrocyte bricks, (B1CB1) group resulted in persistent chondrogenesis with appropriate morphology and adequate central nutritional perfusion without ossification. The related mechanism is that increased expression of Sox9 in the B1C1 group promoted chondrogenesis and inhibited the osteogenesis of BMSCs through upregulating Col-II as well as downregulating RUNX2 and downstream of Col-X and Col-I by upregulating Nkx3.2. This study demonstrated that BMSCs/chondrocyte bricks 1:1 should be a suitable ratio and the Sox9-Nkx3.2-RUNX2 pathway was a related mechanism which played an important role in the niche for stable chondrogenesis of BMSCs constructed by chondrocyte bricks and PRP. SN - 1687-966X UR - https://doi.org/10.1155/2020/5492059 DO - 10.1155/2020/5492059 JF - Stem Cells International PB - Hindawi KW - ER -