TY - JOUR A2 Najimi, Mustapha AU - Kim, Miyeon AU -权，Ji Hye AU - Bae, Yun Kyung AU - Kim, gehye AU - Um, Soyoun AU - Ha, Jueun AU - Choi, Soo Jin AU - Oh, Wonil AU - Jin，惠金PY - 2020 DA - 2020/01/23 TI -可溶性PTX3人类脐带血液的间充质干细胞变弱氧肺损伤通过激活巨噬细胞极化在新生大鼠模型SP - 1802976六世- 2020 AB -治疗治疗各种炎症相关的疾病使用间充质干细胞(msc)近年有所增加,因为这些细胞的旁分泌作用,而是显示了一些局限性。首先，基于msc的疗法表现出不同的疗效;因此，应该确定生物标志物来确定哪些人可能从这些候选治疗药物中获益。其次，对其治疗作用的机制了解甚少。为了评价人脐血源性骨髓间充质干细胞(UCB-MSCs)对巨噬细胞的影响，我们将脂多糖(LPS)诱导的巨噬细胞NR8383细胞株在脐血源性骨髓间充质干细胞上共培养。我们发现UCB-MSCs介导了M1巨噬细胞向M2极化的改变。为了确定抗炎作用下的旁分泌作用，我们使用基于生物素标签的507抗体阵列检测暴露于脂多糖刺激的NR8383细胞中的uc - mscs的分泌情况。在分泌的蛋白中，我们选择了pentraxin相关蛋白PTX3/tumor necrosis factor-inducible gene 14蛋白(PTX3)，研究其与UCB-MSCs在巨噬细胞极化中的关系。我们发现炎症条件下UCB-MSCs分泌人PTX3，并通过Dectin-1受体激活NR8383细胞中MSK1/2磷酸化信号增强M2巨噬细胞标志物。 Accordingly, knockdown of PTX3 in UCB-MSCs significantly attenuated their therapeutic effects in a neonatal hyperoxic lung injury resulting in reduced survival, lung alveolarization, M2 marker expression, Dectin-1 levels, anti-inflammatory cytokines, and improved M1 marker expression and inflammatory cytokines compared to control MSC-injected rats. UCB-MSCs show therapeutic potential by controlling macrophage polarization. Interestingly, higher PTX3 levels in UCB-MSCs induced greater improvement in the therapeutic effects than lower PTX3 levels. Collectively, PTX3 is a potential marker with critical paracrine effects for predicting the therapeutic potential of MSC therapy in inflammatory diseases; quality control assessments using PTX3 may be useful for improving the therapeutic effects of UCB-MSCs. SN - 1687-966X UR - https://doi.org/10.1155/2020/1802976 DO - 10.1155/2020/1802976 JF - Stem Cells International PB - Hindawi KW - ER -