TY - JOUR AU - Moncada-Saucedo, Nidia K. AU - Marino-Martínez, Iván A. AU - Lara-Arias, Jorge AU - Romero-Díaz, Víktor J. AU - Camacho, Alberto AU - Valdés-Franco, J. A. AU - Pérez-Silos, Vanessa AU - García-Ruiz, Alejandro AU - Lin, Hang AU - Tuan, Rocky S. AU - Ramos-Payán, Rosalío AU - Lara-Banda, M. AU - Ortiz-Lopez, Rocio AU - Rojas-Martinez, Augusto AU - Fuentes-Mera, Lizeth PY - 2019 DA - 2019/04/18 TI - A Bioactive Cartilage Graft of IGF1-Transduced Adipose Mesenchymal Stem Cells Embedded in an Alginate/Bovine Cartilage Matrix Tridimensional Scaffold SP - 9792369 VL - 2019 AB - Articular cartilage injuries remain as a therapeutic challenge due to the limited regeneration potential of this tissue. Cartilage engineering grafts combining chondrogenic cells, scaffold materials, and microenvironmental factors are emerging as promissory alternatives. The design of an adequate scaffold resembling the physicochemical features of natural cartilage and able to support chondrogenesis in the implants is a crucial topic to solve. This study reports the development of an implant constructed with IGF1-transduced adipose-derived mesenchymal stem cells (immunophenotypes: CD105⁺，CD90⁺，CD73⁺，CD14^-，和cd34^-）嵌入一个由培养的藻酸盐/碾磨牛脱细胞的膝盖材料组成的支架中组成 体外28天（3CI）。组织学分析证明了由DE Novo致密细胞外基质包围的软骨细胞基因组分布，其具有均衡比例的胶原蛋白II和I和大量的硫酸化蛋白转基因，这也证明了足够的细胞增殖和分化。该移植物也在类似于天然膝关节软骨的机械性能。修改后的伯尔尼/ o'driscoll标度表明，3CI植入物的得分明显高于缺乏使用IGF1转导的细胞的2CI植入物（16/18与14/18），代表适合的优质工程软骨 体内测试。本研究表明，这种移植物类似于典型的透明软骨的若干特征，并将是软骨再生的临床前研究的原因。SN - 1687-966X UR - https://doi.org/10.1155/2019/9792369 Do - 10.1155 / 2019/9792369 JF - 干细胞国际PB - Hindawi Kw - ER -