y - JOUR AU - Nguyen, Van Thi AU - Tessaro, Irene AU - Marmotti, Antonio AU - Sirtori, Camilla AU - Peretti, Giuseppe M. AU - Mangiavini, Laura PY - 2019 DA - 2019/05/02 TI -收获部位是否影响间充质干细胞成骨潜能?全髋关节置换术(THA)是骨科领域最常见的手术程序之一。种植体与天然骨的骨融合是获得最佳结果的必要条件;因此，植体周围的患者骨质量(即骨柄)是至关重要的。然而，在某些情况下，骨存量不足，需要采用富含多能细胞的自体移植物(即从髂骨、股骨头或从髋臼获取的软骨下骨)或同种异体冷冻骨来改善。目前尚不清楚收集部位是否会影响这些细胞的成骨潜能。因此，我们的目的是描述和比较收集自骨髓、髋臼软骨下骨和股骨头小梁骨的多能细胞，重点关注成骨分化。三个来源的细胞具有成纤维细胞样表型，表面抗原CD73、CD90和CD105表达，CD11b、CD34和CD45阴性。虽然这些细胞都能被诱导分化为成骨细胞、软骨细胞和脂肪细胞，但它们表现出不同的分化潜能。在成骨分化条件下，髋臼细胞的钙沉积量最低，而骨髓和股骨细胞的钙沉积量明显增加。 These findings were confirmed by quantitative polymerase chain reaction (qPCR). In chondrogenic and adipogenic conditions, bone marrow cells possessed a predominant differential capacity compared with the others, illustrated by high collagen type II expression together with a cartilage-like lacuna structure and the presence of fat-specific markers, respectively. To our knowledge, this is the first study comparing and demonstrating that the progenitor cells obtained from diverse surgical sites in hip replacement procedure share common characteristics of MSC but differ about plasticity and may provide rational for clinical application in cell therapy and bone grafting. The project number L1033 is registered with ClinicalTrials.gov NCT03369457．SN - 1687-966X UR - https://doi.org/10.1155/2019/9178436 DO - 10.1155/2019/9178436 JF - Stem Cells International PB - Hindawi KW - ER -