TY - JOUR A2 - Volarevic，弗拉季AU - 埃瓦里斯托 - 门多萨，法比亚纳AU - Sardella - 席尔瓦，加布里埃拉AU - 葛西不伦瑞克，泰丝花枝AU - 坎波斯，拉奎尔·玛丽亚·佩雷拉AU - Domizi，巴勃罗AU - 圣地亚哥，马塞洛Felippe AU -德梅洛李嘉欣，里卡多·奥古斯托AU - 门德斯 - 奥特罗，罗萨莉娅AU - 里贝罗，雷森迪，维克多图略AU - 皮门特尔 - 科埃略，佩德罗莫雷诺PY - 2019 DA - 2019年8月19日TI - 大鼠骨髓间充质基质的预处理7692973 VL - - 2019 AB - 与Toll样受体激动剂SP细胞骨髓来源的间充质基质细胞（BM-MSC的）是可感知环境，适应它们的调节功能，以不同的条件的动态细胞。因此，BM-MSC的治疗潜力可通过预处理旨在修改它们的旁分泌作用的策略来调制。虽然大鼠的BM-MSC（RBM-MSCS）已在临床前研究得到了广泛的测试，大多数预处理研究已经使用人类和小鼠的BM-MSC。在此，我们研究了代rBM-MSC的是否修改其表型和功能的旁分泌响应Toll样受体（TLR）激动剂。数据显示，代rBM-MSC表达TLR3，TLR4，和MDA5 mRNA和能够内在化聚肌胞苷酸（聚（I：C）），一个TLR3 / MDA5激动剂。rBM-MSCs were then stimulated with Poly(I:C) or with lipopolysaccharide (LPS, a TLR4 agonist) for 1 h and were grown under normal culture conditions. LPS or Poly(I:C) stimulation did not affect the viability or the morphology of rBM-MSCs and did not modify the expression pattern of key cell surface markers. Poly(I:C) did not induce statistically significant changes in the release of several inflammatory mediators and VEGF by rBM-MSCs, although it tended to increase IL-6 and MCP-1 secretion, whereas LPS increased the release of IL-6, MCP-1, and VEGF, three factors that were constitutively secreted by unstimulated cells. The neurotrophic activity of the conditioned medium from unstimulated and LPS-preconditioned rBM-MSCs was investigated using dorsal root ganglion explants, showing that soluble factors produced by unstimulated and LPS-preconditioned rBM-MSCs can stimulate neurite outgrowth similarly, in a VEGF-dependent manner. LPS-preconditioned cells, however, were slightly more efficient in increasing the number of regrowing axons in a model of sciatic nerve transection in rats. In conclusion, LPS preconditioning boosted the production of constitutively secreted factors by rBM-MSCs, without changing their mesenchymal identity, an effect that requires further investigation in exploratory preclinical studies. SN - 1687-966X UR - https://doi.org/10.1155/2019/7692973 DO - 10.1155/2019/7692973 JF - Stem Cells International PB - Hindawi KW - ER -