TY - JOUR A2 - 黄，永灿AU - 他，永滨AU - 刘晟耀AU - 邓，宋允AU - 旷，李鹏AU - 徐，邵勇AU - 李喆AU -徐雷AU - 刘，魏盟 - 镍，郭鑫PY - 2019 DA - 2019年7月7日TI - 机械牵张促进骨髓间充质干促红细胞生成素诱导SP细胞的成骨分化 - 1839627 VL - 2019 AB - 介绍。促红细胞生成素的（EPO）对骨髓的行为的影响间充质干细胞进行机械拉伸（骨髓干细胞）仍不清楚。因此，本研究的目的是建立EPO刺激对大鼠BMSCs剂量反应效果和调查机械拉伸与EPO上的增殖和骨髓基质细胞的成骨分化组合的影响。 材料与方法。增殖和大鼠BMSCs的成骨细胞分化进行了检查，并使用EPO与不同浓度的比较。Thereafter, BMSCs were subjected to 10% elongation using a Flexcell strain unit, combined with 20 IU/ml EPO. The proliferation of BMSCs was detected by Cell Counting Kit-8, colony formation assay, and cell cycle assay; meanwhile, the mRNA expression levels of Ets-1, C-myc, Ccnd1, and C-fos were detected by reverse transcription and real-time quantitative PCR (qPCR). The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. The role of the extracellular signal-regulated kinases 1/2 (ERK1/2) in the osteogenesis of BMSCs stimulated by mechanical stretch combined with 20 IU/ml EPO was examined by Western blot. 结果。Our results showed that effects of EPO on BMSCs included a dose-response relationship, with the 20 IU/ml EPO yielding the largest. Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. The increase in ALP, mineral deposition, and osteoblastic genes induced by the mechanical stretch–EPO combination was inhibited by U0126, an ERK1/2 inhibitor. 结论。EPO能促进骨髓干细胞的增殖和成骨细胞分化，并且当与机械拉伸相结合得到增强这些效果。潜在的机制可能与ERK1 / 2的信号传导途径的活化。SN - 1687-966X UR - https://doi.org/10.1155/2019/1839627 DO - 10.1155 /一百八十三万九千六百二十七分之二千○一十九JF - 干细胞国际PB - Hindawi出版KW - ER -