TY - JOUR A2 - 彭宁斯，纱丽AU - 费雷拉，安德烈·达丰塞卡·AU - 达库尼亚群岛，Pricila达席尔瓦AU - Carregal，弗吉尼亚·门德斯AU - 席尔瓦，Priscila德槐达盟 - 米兰达，马塞洛科蒂尼奥德AU - Kunrath - 利马，玛丽安娜AU - 德梅洛，玛丽安伊莎贝拉阿布雷乌AU - Faraco，卡米拉克里斯蒂娜·弗拉加AU - 巴博萨，琼娜·洛巴托AU - Frezard，弗雷德里克AU - 雷森迪，维维安AU - 罗德里格斯，米歇尔区文诗 - 云，阿尔弗雷多·米兰达去AU - 戈麦斯，Dawidson阿西斯PY - 2017 DA - 2017年11月5日TI - 胞外囊泡从脂肪间充质干/基质细胞加速迁移和激活Akt途径在人角质形成细胞和成纤维细胞的miR独立-205活性的SP - 9841035 VL - 2017 AB - 间充质干/基质细胞（MSCs）是有希望在细胞治疗工具。他们分泌携带不同类别，可以促进皮肤修复的分子细胞外囊泡（电动汽车），但其机理知之甚少。皮肤伤口愈合是一个复杂的过程，需要的几个信号传导途径和细胞类型，包括角质形成细胞和成纤维细胞的活性。在这项研究中，我们探讨脂肪组织MSC衍生的电动汽车能否加速迁移和角质形成细胞和成纤维细胞增殖，激活AKT通路，促进伤口愈合 体内。此外，我们评估如果EV影响的miR-205依赖性。We found that MSC EVs had an average diameter of 135 nm. Keratinocytes and fibroblasts exposed to EVs exhibited higher levels of proliferation, migration, and AKT activation. Topical administration of EVs accelerated skin wound closure. Knockdown of miR-205 decreased AKT phosphorylation in fibroblasts and keratinocytes, whereas migration was decreased only in keratinocytes. Moreover, knockdown of miR-205 failed to inhibit AKT phosphorylation in fibroblasts and keratinocytes exposed to EVs. About the mechanism of EV effects, we found that incubation with EVs prevented inhibition of AKT activation by miR-205 knockdown, suggesting that EVs activate AKT independently of miR-205. In conclusion, we demonstrated that EVs are a promising tool for wound healing. SN - 1687-966X UR - https://doi.org/10.1155/2017/9841035 DO - 10.1155/2017/9841035 JF - Stem Cells International PB - Hindawi KW - ER -