TY -的A2 Nalapareddy Kodandaramireddy AU -Neckel, Peter Helmut AU - Mohr, Roland AU - Zhang, Ying AU - Hirt, Bernhard AU - Just, Lothar PY - 2016 DA - 2015/11/30 TI - Comparative Microarray Analysis of Proliferating and Differentiating Murine ENS Progenitor Cells SP - 9695827 VL - 2016 AB - Postnatal neural progenitor cells of the enteric nervous system are a potential source for future cell replacement therapies of developmental dysplasia like Hirschsprung’s disease. However, little is known about the molecular mechanisms driving the homeostasis and differentiation of this cell pool. In this work, we conducted Affymetrix GeneChip experiments to identify differences in gene regulation between proliferation and early differentiation of enteric neural progenitors from neonatal mice. We detected a total of 1333 regulated genes that were linked to different groups of cellular mechanisms involved in cell cycle, apoptosis, neural proliferation, and differentiation. As expected, we found an augmented inhibition in the gene expression of cell cycle progression as well as an enhanced mRNA expression of neuronal and glial differentiation markers. We further found a marked inactivation of the canonical Wnt pathway after the induction of cellular differentiation. Taken together, these data demonstrate the various molecular mechanisms taking place during the proliferation and early differentiation of enteric neural progenitor cells. SN - 1687-966X UR - https://doi.org/10.1155/2016/9695827 DO - 10.1155/2016/9695827 JF - Stem Cells International PB - Hindawi Publishing Corporation KW - ER -