TY -的A2 Vanella卢卡AU - Bianchessi,玛尔塔非盟-陈,宇文非盟- Durgam Sushmitha盟——Pondenis冬青AU -斯图尔特,马修PY - 2016 DA - 2015/12/29 TI -纤维母细胞生长因子2对马滑液Chondroprogenitor扩张和软骨形成SP - 9364974六世- 2016 AB -间充质干细胞已确定滑液的几个物种。本研究进行了描述chondroprogenitor (CP)马滑液细胞(SF)和决定的影响纤维母细胞生长因子2 (FGF-2) SF-CP单层核扩散和随后的软骨形成。我们假设FGF-2刺激SF-CP核扩散和postexpansion软骨形成。科幻吸入收集从成年马关节。克隆形成单位(CFU)分析中主要的文化。起初,SF-cells被播种在低密度,有或没有FGF-2。单层扩张和串行immunophenotyping后,细胞被转移到chondrogenic小球的文化。分析了颗粒chondrogenic mRNA表达和软骨基质分泌。有一个平均的59.2 CFU /毫升的科幻小说。FGF-2增加人口倍增的数量在两个单层段落和一半人口翻倍。 FGF-2 did not alter the immunophenotype of SF-CPs during monolayer expansion, nor did FGF-2 compromise chondrogenesis. Hypertrophic phenotypic markers were not expressed in control or FGF-2 groups. FGF-2 did prevent the development of a “fibroblastic” cell layer around pellet periphery. FGF-2 significantly accelerates in vitro SF-CP expansion, the major hurdle to clinical application of this cell population, without detrimentally affecting subsequent chondrogenic capacity. SN - 1687-966X UR - https://doi.org/10.1155/2016/9364974 DO - 10.1155/2016/9364974 JF - Stem Cells International PB - Hindawi Publishing Corporation KW - ER -