Ty - Jour A2 - Ballini，Andrea Au - Tan，Chengbo Au - Shichinohe，Hideo Au - Wang，Zifeng Au - Hamauchi，Shuji Au - Abumiya，Takeo Au - Nakayama，Nakayama，Naoki Au - Kazumata，Ken Au - Ito，Tsuneo Au -Kudo，Kohsuke Au - Takamoto，Shigeru Au - Houkin，Kiyohiro Py - 2016 DA - 2016/10/20 - 2016/10/20 TI - 人骨髓基质细胞培养物与异种血小板裂解物 - 细胞疗法对中风SP的补充 - 6104780 VL- 2016年AB - 目前，对人骨髓基质细胞（HBMSCs）的兴趣越来越令人兴趣，作为对脑卒中的再生治疗。本研究的目的是评价HBMSC培养物与同种异体血小板裂解物（PLS）的可行性和有效性。血小板浓缩物（PC）从健康的志愿者收获并制成单一供体衍生的PL（SPL）。PL混合物（MPL）由三种不同的SPL制成。通过酶联免疫吸附测定（ELISA）检测一些生长因子和血小板细胞表面抗原。分析用10％PL培养的HBMSCS用于其增殖性潜在，表面标志物和核型。将细胞与超顺磁性氧化铁（SPIO）试剂一起温育并注入猪脑中。进行MRI和组织学分析。 Consequently, nine lots of sPL and three mPL were prepared. ELISA analysis showed that PL contained adequate growth factors and a particle of platelet surface antigens. Cell proliferation capacity of PLs was equivalent to or higher than that of fetal calf serum (FCS). No contradiction in cell surface markers and no chromosomal aberrations were found. The MRI detected the distribution of SPIO-labeled hBMSCs in the pig brain. In summary, the hBMSCs cultured with allogeneic PL are suitable for cell therapy against stroke. SN - 1687-966X UR - https://doi.org/10.1155/2016/6104780 DO - 10.1155/2016/6104780 JF - Stem Cells International PB - Hindawi Publishing Corporation KW - ER -