TY - JOUR A2 - 帕尔，Rajarshi AU - 赫弗南，科里AU - 苏美尔，玉山AU - Malaver - 奥尔特加，路易斯F. AU - 维尔马，保罗J. PY - 2012 DA - 2012/04/26 TI - cMyc的时间要求蛋白重编程小鼠成纤维细胞SP - 541014 VL - 2012 AB - OCT4，SOX2，KLF4的外源表达，和cMyc力哺乳动物体细胞通过胚胎干细胞的分子和表型特征，与谱系相关基因所需要的抑制开始（e.g., THY1在鼠标）。虽然从重新编程鸡尾酒最小化省略cMyc的风险不受控制的增殖，其排斥导致在重编程效率倍减量。因此，具有取代的cMyc转基因的可行性（非整合型）重组的“PTAT-mcMyc”蛋白质递送进行评价，而不损害重编程效率或该多能性表型。纯化和semisoluble /颗粒PTAT-mcMyc的输送保持的Oct4-GFP⁺集落形成（即，重编程效率），而通过各种标准支持多能性。Differential repression of Thy1 by pTAT-mcMyc ± Oct4, Sox2, and Klf4 (OSK) suggested differential (and non-additive) mechanisms of repression. Extending these findings, attempts to enhance reprogramming efficiency through a staggered approach (prerepression of Thy1) failed to improve reprogramming efficiency. We consider protein delivery a useful tool to decipher temporal/molecular events characterizing somatic cell reprogramming. SN - 1687-966X UR - https://doi.org/10.1155/2012/541014 DO - 10.1155/2012/541014 JF - Stem Cells International PB - Hindawi Publishing Corporation KW - ER -