TY - JOUR A2 - 王，Heuy晴赫蒂AU - 扬，荆AU - 王，王尽美AU - 谷，平AU - 你，十乔安AU - 克拉森，亨利PY - 2012 DA - 2012/04/23 TI -猫的神经祖细胞I：下定义的培养条件SP长期扩展 - 108340 VL - 2012 AB - 神经祖细胞猫起源（cNPCs）的（筹备）已经证明，在移植实验工具，但难以在培养中生长的超越1个月时间帧。在这里，我们使用一个富集的，无血清基础培养基（Ultraculture）并报告这些细胞的成功的长期传播。Primary cultures were derived from fetal brain tissue and passaged in DMEM/F12-based or Ultraculture-based proliferation media, both in the presence of EGF + bFGF. Cells in standard DMEM/F12-based medium ceased to proliferate by 1-month, whereas the cells in the Ultraculture-based medium continued to grow for at least 5 months (end of study) with no evidence of senescence. The Ultraculture-based cultures expressed lower levels of progenitor and lineage-associated markers under proliferation conditions but retained multipotency as evidenced by the ability to differentiate into neurons and glia following growth factor removal in the presence of FBS. Importantly, later passage cNPCs did not develop chromosomal aberrations. SN - 1687-966X UR - https://doi.org/10.1155/2012/108340 DO - 10.1155/2012/108340 JF - Stem Cells International PB - Hindawi Publishing Corporation KW - ER -