TY - JOUR A2 - Bunnell, Bruce A. AU - Vanikar, A. V. AU - Dave, S. D. AU - Thakkar, U. G. AU - Trivedi, H. L. PY - 2010 DA - 2010/12/20 TI - Cotransplantation of Adipose Tissue-Derived Insulin-Secreting Mesenchymal Stem Cells and Hematopoietic Stem Cells: A Novel Therapy for Insulin-Dependent Diabetes Mellitus SP - 582382 VL - 2010 AB - 目标。胰岛素依赖型糖尿病（IDDM）被认为是一种自身免疫性疾病与干扰葡萄糖/胰岛素代谢，需要终身胰岛素替代疗法（IRT），30％的患者发展为终器官衰竭。我们提出我们的来自脂肪组织的胰岛素分泌的间充质干细胞的共移植的经验（IS-AD-MSC）和培养骨髓（CBM），其IRT为这些患者。 方法。这是一个前瞻性的开放标记的临床试验，以IS-AD-MSC + CBM联合移植治疗IDDM，在11个批准的机构审查委员会知情同意后的测试有效性和安全性（男性：女性：7：4）患者with 1–24-year disease duration, in age group: 13–43 years, on mean values of exogenous insulin requirement of 1.14 units/kg BW/day, glycosylated hemoglobin (Hb1Ac): 8.47%, and c-peptide levels: 0.1 ng/mL. Intraportal infusion of xenogeneic-free IS-AD-MSC from living donors, subjected to defined culture conditions and phenotypically differentiated to insulin-secreting cells, with mean quantum: 1.5 mL, expressing Pax-6, Isl-1, and pdx-1, cell counts: 2.1 × 10 3 / μL，CD45^-/ 90⁺/ 73⁺:40/30.1%, C-Peptide level:1.8 ng/mL, and insulin level: 339.3  IU/mL with CBM mean quantum: 96.3 mL and cell counts: 28.1 × 10 3 / μL，CD45^-/ 34⁺：0.62％，进行了。 结果。所有成功被移植，没有任何不良反应。Over mean followup of 23 months, they had a decreased mean exogenous insulin requirement to 0.63 units/kgBW/day, Hb1Ac to 7.39%, raised serum c-peptide levels to 0.38 ng/mL, and became free of diabetic ketoacidosis events with mean 2.5 Kg weight gain on normal vegetarian diet and physical activities. 结论。这与胰岛素分泌-AD-MSC + CBM具有相对简单的技术治疗IDDM安全和有效地的第一份报告。SN - 1687-966X UR - https://doi.org/10.4061/2010/582382 DO - 10.4061 /五十八万二千三百八十二分之二千○一十JF - 干细胞国际PB - SAGE-Hindawi出版访问研究KW - 呃 -