TY - JOUR A2 - Johnston, S. L. AU - Chidekel, Aaron AU - Zhu, Yan AU - Wang, Jordan AU - Mosko, John J. AU - Rodriguez, Elena AU - Shaffer, Thomas H. PY - 2012 DA - 2012/09/03 TI - The Effects of Gas Humidification with High-Flow Nasal Cannula on Cultured Human Airway Epithelial Cells SP - 380686 VL - 2012 AB - Humidification of inspired gas is important for patients receiving respiratory support. High-flow nasal cannula (HFNC) effectively provides temperature and humidity-controlled gas to the airway. We hypothesized that various levels of gas humidification would have differential effects on airway epithelial monolayers. Calu-3 monolayers were placed in environmental chambers at 37°C with relative humidity (RH) < 20% (dry), 69% (noninterventional comparator), and >90% (HFNC) for 4 and 8 hours with 10 L/min of room air. At 4 and 8 hours, cell viability and transepithelial resistance measurements were performed, apical surface fluid was collected and assayed for indices of cell inflammation and function, and cells were harvested for histology ( N = 6. /健康）状况）。随着时间的推移降低了Transepithelial抗性和细胞活力（ P. < 0.001 ）HFNC和干组（ P. < 0.001 ）。在干组中，总蛋白质分泌在8小时内增加（ P. < 0.001 ）。干燥组中白细胞素（IL）-6和IL-8的分泌大于其他组8小时（ P. < 0.001 ）。组织学分析显示干组织随时间造成的损伤。这些数据表明，暴露于低湿度导致降低的上皮细胞功能和增加的炎症。SN - 2090-1836 UR - https://doi.org/10.1155/2012/380686 Do - 10.1155 / 2012/380686 JF - 肺医药PB - Hindawi Publishing CorporationKW - ER -