TY -的A2 Lloret安娜盟——汉Guo-Jie AU - Min, Xiang-Zhen AU - Ma, Shuang-Shuang AU -叮,栓AU -王,Xiu-Qin PY - 2022 DA - 2022/12/07 TI - Xuesaitong结合Dexmedetomidine改善脑缺血再灌注损伤大鼠通过激活Keap1 / Nrf2信号和Mitophagy海马组织中SP - 5126042六世- 2022 AB -缺血性中风是最常见的脑血管疾病死亡率高,预后不良,和脑缺血再灌注(CI / R)损伤是主要杀手。在这里,我们试图探索Xuesaitong的效果和机制(XST)结合dexmedetomidine(敏捷)CI / R损伤的老鼠。首先,CI / R损伤鼠模型构造通过大脑中动脉阻塞(MCAO)方法和治疗XST和敏捷单独或组合。然后,在第五和第十天的治疗,神经损伤评估使用修改后的神经严重程度评分(mns) 8-arm径向迷宫测试(8 armt),小说对象识别测试(NORT),和恐惧条件反射试验(FCT)。)进行染色观察海马的病理变化。ELISA和相关工具被用来评估在海马单胺神经递质和抗氧化剂酶活动。ATP,线粒体膜电位水平,存在线粒体功能相关基因的测定来评估在海马线粒体功能和免疫印迹确定Keap1 / Nrf2信号通路和mitophagy-related蛋白质表达。结果表明,XST结合敏捷显著降低mns,改善空间记忆和学习赤字,和增强的恐惧记忆和认知记忆能力在CI / R老鼠,这是优于单药治疗。此外,XST结合敏捷治疗改善海马组织病理学损伤;显著增加的水平单胺神经递质,神经营养因子,ATP,线粒体膜电位; and upregulated the activities of antioxidant enzymes and the expression of mitophagy-related proteins in the hippocampus of CI/R rats. XST combined with Dex treatment also activated the Keap1/Nrf2 signaling and upregulated the protein expression of downstream antioxidant enzymes HO-1 and NQ. Altogether, this study showed that a combination of XST and Dex could activate the Keap1/Nrf2 signaling and mitophagy to protect rats from CI/R-related neurological impairment. SN - 1942-0900 UR - https://doi.org/10.1155/2022/5126042 DO - 10.1155/2022/5126042 JF - Oxidative Medicine and Cellular Longevity PB - Hindawi KW - ER -