TY -的A2 Sokovi滨AU -哈西姆,Heba r . PY - 2020 DA - 2020/11/23 TI -再生和抗氧化性能的进程和自体的血浆疗法可以储备衰老大鼠角膜的模型SP - 4127959六世- 2020 AB -老化是一种自然进步的细胞的生物学功能下降。角膜与年龄相关的变化会影响其折射光或自我修复的能力。富含血小板血浆(PRP)一个有前途的角色在再生医学和证明它的效力在多个领域,但在角膜老化尚未阐明。目前的工作是评估再生PRP在角膜衰老大鼠的抗氧化作用。老鼠被分为两个主要的组:(GI)成人群体和(GII)岁组。成人群体分为GIa(成年鼠),直布罗陀海峡(adult-saline治疗)和新加坡政府投资公司(adult-PRP治疗)。老年人群体分为GIIa(年龄大鼠)和GIIb(年龄,PRP治疗)。PRP是由一个单一的结膜下注射。10天后、组织学、超微结构、免疫组织化学和morphometrical调查。岁组的检查角膜部分显示角膜上皮变薄,表面脱落的上皮细胞失去desmosomal结,和不规则性肾小球膜。 Disorganized widely spaced collagen bundles and neovascularization were detected in corneal stroma associated with thickening in Descemet’s membrane. Ultrastructural examination revealed shrunken hyperchromatic nuclei, swollen mitochondria, and scanty cytoplasm with a strong nuclear reaction for caspase-3 immunostaining. Moreover, antioxidant/free radicals’ imbalance was detected by the increase of malondialdehyde (MDA) level with a decrease of glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels. In contrast, GIIb (aged, PRP treated) section examination revealed a restoration of the thickness of the corneal epithelial layer and Descemet’s membrane with an amendment of collagen fiber regularity that is associated with weak nuclear reaction to caspase-3 and recovery of the balance in the redox state. These findings proved the effectiveness of PRP as a promising regenerative treatment for the age-associated changes in the cornea. SN - 1942-0900 UR - https://doi.org/10.1155/2020/4127959 DO - 10.1155/2020/4127959 JF - Oxidative Medicine and Cellular Longevity PB - Hindawi KW - ER -