TY -的A2 Tylzanowski Przemko AU - Gęgotek,阿格涅斯卡非盟- domingue,佩德罗盟——Skrzydlewska Elżbieta PY - 2020 DA - 2020/11/05 TI -天然植物外源抗氧化剂防御人类皮肤成纤维细胞蛋白质组的变化被UVA辐射SP - 3216415六世- 2020 AB -日常接触皮肤的UVA辐射导致氧化修饰细胞组件和生物分子。这些包括蛋白质参与成纤维细胞的新陈代谢和cytoprotection,和他们的修改会引起细胞功能的中断和皮肤疾病的发展。因此,仍然是一个需要高度活跃cytoprotective化合物具有抗氧化特性。本研究的目的是探讨抗坏血酸对芦丁的活动的影响对UVA-induced人类成纤维细胞的蛋白质组的变化。所有分析都进行成纤维细胞培养在一个三维系统暴露在UVA辐射和孵化芦丁和抗坏血酸。他们使用nano-HPLC蛋白质组剖面进行了分析,显示150之间的蛋白质的表达显著改变治疗条件。UVA辐射导致82蛋白的表达变化。然而,这些改变被减轻分别芦丁和抗坏血酸(分别为23和25蛋白质)和芦丁和抗坏血酸(23)的蛋白质。UVA辐射导致蛋白质的upregulation参与基因表达,蛋白质的差别催化过程和抗氧化途径,对这些绑定活动。然而,芦丁和抗坏血酸单独或一起反驳这些不同程度的变化。 Moreover, rutin and ascorbic acid stimulated fibroblasts irradiated by UVA to increase the expression of the signalling molecules responsible for the opening of the transmembrane channels. In the context of the results obtained, the observed cytoprotective effect of the cooperation of rutin and ascorbic acid results not only from the overlapping properties of the compounds. The effect of rutin alone is probably inhibited by its limited bioavailability. Therefore, its interaction with ascorbic acid increases membrane penetration and improves the cytoprotective effect on skin fibroblasts. SN - 1942-0900 UR - https://doi.org/10.1155/2020/3216415 DO - 10.1155/2020/3216415 JF - Oxidative Medicine and Cellular Longevity PB - Hindawi KW - ER -