TY - JOUR A2 - 雅科夫列维奇，弗拉基米尔AU - 邵婧盟 - 柳，宜坳 - 王欢AU - 洛，云AU - 陈，雷PY - 2020 DA - 2020年5月27日TI - 一个集成的粪便微生物组和在2型糖尿病大鼠代谢组学揭示从乙酸乙酯提取物的宿主 - 微生物代谢轴降糖效果苦参SP - 1805418 VL - 2020 AB - 2型糖尿病（T2DM）是一种慢性代谢性疾病。 苦参（ 苦参），又称苦参，是已被用于预防和无论是在民间医药和医疗机构治疗2型糖尿病中国著名的中草药。然而，其作用机制尚不清楚。在这项研究中，药效学作用 苦参EtOAc萃取物（SFE）上高脂肪饮食和低剂量STZ诱导T2DM大鼠进行了研究。粪便代谢组学分析和16S rRNA基因测序用于确定T2DM和SFE治疗对肠道菌群和代谢的影响。Based on the consistency of the results of metabolic pathways in metabolomics analysis and phylogenetic investigation of communities by reconstruction of unobserved state (PICRUSt) analysis of 16S rRNA gene sequencing, the level of metabolites and the operational taxonomic units of gut bacteria were combined, and Spearman’s analysis was implemented. Our data showed that SFE significantly decreased fasted blood glucose levels and improved lipid profile, glycosylated serum protein, glycosylated hemoglobin index, and pancreas damage. Metabolomics and 16S rRNA gene sequencing analysis indicated gut bacteria disorder, disturbed lipid metabolism, carbohydrate metabolism, and especially amino acid metabolism in T2DM and that SFE can regulated these metabolic pathways through the influence on gut bacteria. Spearman’s analysis indicated that the amino acid metabolism that included tryptophan, branched chain amino acid, aromatic amino acid, beta-alanine, and glycine, serine and threonine metabolism, lipid metabolism, including lysophosphatidylcholines and lysophosphatidylethanolamines, primary bile acid and linoleic acid metabolism, carbohydrate metabolism, and nucleotide metabolism positively correlated with Faecalibacterium， Flexispira， Phascolarctobacterium， 普雷沃， 罗斯氏和 [普雷沃]。此外，精氨酸和脯氨酸代谢，类固醇激素，类固醇的生物合成，和鞘脂代谢的正相关 乳酸杆菌， Oscillospira， Parabacteroides， 瘤胃球菌属和 链球菌。综上所述，我们推测SFE可能通过介导宿主 - 微生物代谢轴对2型糖尿病的效果。SFE用于治疗2型糖尿病的multiomics的探索有望为临床治疗提供参考。SN - 1942-0900 UR - https://doi.org/10.1155/2020/1805418 DO - 10.1155 /一百八万五千四百十八分之二千〇二十〇JF - 氧化医学和细胞寿命PB - Hindawi出版KW - ER -